* [[ActionGirl Tex]] in ''Machinims/RedVsBlue'' gets this moment when Donut lobs a plasma grenade over an ''incredible'' distance, square into the cockpit of the tank she was driving.
** Everyone is one the receiving end of this at least once, although their reaction is less "oh crap" and more "son of a bitch!"
** In ''Recreation'' Episode 12, Donut is cleaning up Blue Base when he's approached by what he thinks is a new Blue soldier (it's actually the previous season's BigBad, [[TheJuggernaut Meta]].) Donut, being [[HorribleJudgeOfCharacter Donut]], tries to strike up a friendly conversation with it, evading its attempts to kill him by near misses. Simmons then arrives, Donut tells him about the new soldier, which leads to this:
---> '''Simmons''': "What? They sent another team member? Why would they do that? That doesn’t make any sense- ''(sees that it's Meta)'' '''OH, FUCK!''' ''(starts to run away)'' Welcometotheneighborhoodseeyoulater!
** ''Red vs. Blue: Revelation'', Chapter 3:
--> '''Washington''': Do I hear a...
--> ''(Grif drives a Warthog jeep [[DynamicEntry through the wall]])''
--> '''Washington''': CAR?!?
** ''Revelations'' Episode 10 is filled with a ton of them from Grif, Sarge, Simmons and Tucker while they're all in the middle of being on the receiving end of a brutal CurbStompBattle at the hands of Tex.
** When The Meta attaches the memory unit containing the recently captured Tex to his armor.
** The Meta is the living personification of this trope. He even has his own [[http://www.youtube.com/watch?v=tw2VDWB-u2Y Theme music]] which makes the Imperial March, Jaws theme and PsychoStrings look tame in comparison.
** And now try [[http://www.youtube.com/watch?v=Lke056oO3cU this.]] If you thought the last one was pants-wettingly terrifying, then this one makes the previous link sound like a frolic in a happy meadow.
** The OhCrap factor doubles down in subsequent seasons as we see the process that took him from "just" an exceptional supersoldier to an unstoppable force of evil.
* TheDementedCartoonMovie: An underwater diver recites the trope by name upon realizing that the "Bubble Fish" he has been tracking is actually [[ActionBomb Zeeky H. Bomb]].
* ''WebAnimation/HappyTreeFriends'' is a total definition of this trope. Nearly every episode has it's Oh Crap moments (save for just a few). In the fanimation "All Work and No Belay", before Lumpy [[spoiler:falls and rolls into a big snowball]], [[GravityIsAHarshMistress he notices what will happen]] and holds up a sign saying "HELP?".
* In the first episode of ''WebAnimation/DSBTInsaniT'', Koden and Asia have a moment when they realize Lisa and Sand Snake are going to crash Koden's party.
** Happens to Koden again in 'The Camping Webisode' when he finds out Killer Monster's name.
* WebAnimation/ReverseJurassicPark: Used word-by-word by Mr. Robustus in the fivequel when he realizes that [[spoiler:the giant aquarium he is standing in front of just cracked]]
* ''Machinima/TeamServiceAnnouncement''
** The RED Team in ''[[http://www.youtube.com/watch?v=U9LdJyuWS9g Objectives]]'' when [[spoiler:they hear the Administrator announce that the payload is nearly at the final checkpoint. And they're on the other side of the map.]]
** The Engineer in ''[[http://www.youtube.com/watch?v=n049JQaXAPE Sentry Knockback]]'' when the Medic blocks his Sentry's gunfire. [[HeroicBSOD He doesn't snap out of it]].
** The RED Engineers in ''[[http://www.youtube.com/watch?v=JHOZNRhKBrI Building Placement]]'' when the Demoman destroys all their sentries... just as BLU Team's cart is about to reach the final checkpoint.
* After explaining the backstory, the various narrators of ''WebAnimation/LoreInAMinute'' send the focus game's protagonist off with a "Have fun!". Their reaction is a very upset ThisIsGonnaSuck-type face.
* The reaction of the [[Creator/FourKidsEntertainment 4Kids CEO]] when he realizes that [[WesternAnimation/SonicSATAM Julian Robotnik]] is about to blow him and his company to kingdom come in [[https://www.youtube.com/watch?v=2qDH1hG7tjE Sonic Shorts]].
--> '''Board Member:''' That is, without a doubt, the most ridiculous idea I've ever heard. NO ONE likes Sonic X! NO ONE! Are you PURPOSELY trying to piss people off?
--> '''CEO:''' Yeah, and what are these so-called people gonna do about it? Nah-ne-nah-ne-nah... (bombs appear outside through window) Oh, crud...
* ''WebAnimation/{{Dreamscape}}'': It happens pretty often, such as when Dylan runs into Ethan, but the biggest example is the groups reaction when Keela goes into her SuperpoweredEvilSide.
* In the [[http://tvtropes.org/pmwiki/pmwiki.php/WesternAnimation/MyLittlePonyFriendshipIsMagic My Little Pony: Friendship is Magic]] version of the song [[https://www.youtube.com/watch?v=X_TMtgjQuZI Don't Mine At Night]], a parody of the Katy Perry tune "Last Friday Night", the main character declares that zombies want to eat him and the character he's singing to. Then, because he's singing this while standing beside said zombies, he follows up with a "[[http://tvtropes.org/pmwiki/pmwiki.php/Main/DoubleTake WaitWHAT?!]]".
* ''WebAnimation/{{RWBY}}''
** [[http://vignette3.wikia.nocookie.net/rwby/images/f/f0/1108_Players_and_Pieces_05494.png/revision/latest?cb=20131205084957 Jaune and Weiss]] when they both realise that [[GravityIsAHarshMistress it's a long way down]].
** On a more serious note, Blake gets one when [[spoiler:she accidentally outs herself as a former terrorist in front of her team]].
** Neo, who is smugly smirking all the way through her battle with Yang, [[spoiler:is stopped short of finishing her by the sudden entrance of a mysterious samurai woman with a huge nodachi and a scary mask]]. The way her eyes narrow and her face droops suddenly is hilarious.
** Another comedic version is the look on Team JNPR's collective faces when Nora's slung pie hits Weiss, setting off their epic FoodFight.