[[caption-width-right:320:His name stands for "[[RecursiveAcronym Beware of BOB]]", [[OhCrap by the way]].]]
!! Original run (Seasons 1 and 2, and FWWM)
* BOB/[[spoiler: Leland Palmer]]
** Everything is tolerable '''but''' his little Creator/FredAstaire dance with the golf club in the episode where [[spoiler:he's been driving around ALL DAY with Maddy's body in the trunk]]: there's something just creepingly, inexplicably nightmarish about the sheer randomness of it.
* The end of Episode 14: Whilst Cooper and others are gathered at the roadhouse, [[spoiler: the Giant appears, ominously stating: "It is happening again." The viewer then sees the Palmer household where Leland looks in the mirror and BOB is in the reflection, revealing him to be the killer (and rapist) of his daughter. The implications of this are horrific enough, but almost immediately, Leland/BOB proceeds to claim his next victim.]]
* And [[spoiler: Maddy's]] exceptionally violent (well, for network TV [[SeinfeldIsUnfunny at the time]] anyway) death.
** [[spoiler: As well as the fact that it takes place entirely within the Palmer's living room. Despite all the fantastical elements, the scene doubles as very realistic looking domestic violence.]]
** The [[spoiler: shifting perspectives during this scene whilst Leland is clutching Maddy, between Leland crying and BOB sickeningly kissing her.]]
-->[[spoiler:"Leland says... you're going back... to Missoula... MONTANA!"]]
* Maddy's screams from within the Black Lodge. Talk about NightmareFace.
* [[http://www.youtube.com/watch?v=0PWEqbwh2ow Ronette's Dream]] at the end of [=S02E01=] is a real whopper too.
* The ending of the episode "Coma".
* The words repeated by Waldo. The unpassionate tone only makes the words more disturbing.
* [[spoiler: Leo's awakening]] midway through Season 2.
** [[spoiler: "Bye, wife."]]
* Then of course there's the huge pile of MindScrew / SurrealHorror that essentially makes up the entire last half hour of the final episode.
-->[[http://www.youtube.com/watch?v=IEveQxYv57g&feature=related "How's Annie?"]] ('''Warning: HUGE spoilers!''')
* Mike Gerard's possession and recounting of the 'Fire Walk With Me' poem was particularly chilling. Hats off to Al Strobel for the shift from a kindly shoe salesman to an eerie ancient spirit.
* Harold Smith's [[spoiler:FreakOut when he finds out Donna is secretly trying to take Laura's diary from him]] is really disturbing considering [[spoiler:how he was so friendly before]].
* How about in the second-to-last episode, when Windom Earle returns to his hideout with [[NightmareFace a white face and black teeth]] after [[spoiler:finding the Black Lodge]]?
* Just about 90% of ''Fire Walk With Me'', but notably the Music/DavidBowie [[OneSceneWonder scene.]]
** The [[spoiler: rape scene where Laura finds out Bob possessed her father.]]
* That shadow on the curtain during Cooper's dream at the end of the second episode.
** Apparently, it's supposed to be an [[OminousOwl owl]]. Not that it makes it any less creepy.
* Maddy's [[http://www.youtube.com/watch?v=_B5pE1DEHyk vision]] of BOB.
** Along with Sarah Palmer's in the first season, as seen in the image above.
* [[spoiler: [[SpidersAreScary Leo's (probable) end]].]]
* Ronette Pulaski's first appearance, walking down the bridge in a daze, ropes dangling from her wrists.
* Something about Cooper and Harry looking at Laura Palmer's dead body while the electricity flickered in and out made an otherwise normal (at least, normal for murder dramas) scene absolutely horrifying.
* Ronette's arms slowly raising up as she sleeps alone in her hospital bed.
* Really want to scare yourself? Try playing the Mike and Bob section of Cooper's dream sequence in the German dub.
* Leland's [[spoiler: revelation about BOB's possession and the murder of his daughter [[AdultFear can be this from his perspective.]] ]]
!! The Return
* That ''thing'' in the glass box in the first episode of The Return. And what it does.
** The way the box gets darker and darker before this ghostly pale figure just ''appears'' in it is just extremely unsettling.
* There is a scene in the first episode of season 3 in which a man is crying in a jail cell; the camera pans past him, and there is another man with a beard covered completely in dark gray paint, but whose eyes are extremely white and also TURN SLOWLY TO FACE THE CAMERA.
* The initial discovery of Ruth (and then the complete reveal of her body, [[spoiler: made much worse when you find out the body doesn't even belong to Ruth. ]]) is completely disgusting and wrong looking.
* The pink room Cooper visits [[spoiler: after being thrown out of the Black Lodge.]] The woman within moves in a strange, unearthly fashion, [[EyeScream has no eyes, or even sockets,]] and the door in the back is constantly being angrily pounded upon by her [[NothingIsScarier unseen]] "mother."
* Scenes in the Red Room and the Lodge in general have gotten more intense and unsettling in the revival with the introduction of uncanny CG elements. Any crumb of classiness and subtlety has been striped away, revealing the ever changing labyrinth of nightmares and unforgiving absurdity in much grander detail than ever before.
* [[spoiler: BOB!Cooper is just as terrifying as you'd imagine, if not more so.]] Unlike with [[spoiler: Leland]], he's cold and calculating, yet still positively ruthless. [[spoiler: The scene where he brutally murders Daria, and it's build up which involves holding her down tightly and interrogating her, is chill-inducing.]]
** His introductory scene, in its entirety, is just incredibly eerie and unsettling. It starts with a shot of his car driving through a darkened forest while a bizarre song that consists solely of a menacing drumbeat and inhumanly distorted vocals blares on the soundtrack [[note]] Actually Muddy Magnolias' American Woman, slowed down [[/note]], creating an incredibly ominous tension. Then we meet "Mr. C" and his henchmen, a family of HillbillyHorrors straight out of ''Film/TheHillsHaveEyes'', and the show establishes right away what an evil, dominating presence this character is. It's quite an EstablishingCharacterMoment.
* The scene with the casino manager, just for the wonderfully creepy turn of phrase, "Why do you let him (presumably BOB) make you do these things?".
* The growing revelations from both the new series and the book that Gordon Cole has more power than we knew, and has ties to all sorts of very shady people. He goes from an eccentric father figure to much more of an unknown power, we don't know what he's capable of. Denise even calls him out for his habit of taking in young, wild, attractive agents and getting them involved in spooky stuff, and he replies by threatening her with blackmail; softly and nebulously but definitely a threat.
* [[spoiler:When Cooper's getting of the Black Lodge or at least traveling through another forms of realities, something happens to BOB!Cooper in the real world to the point where he begins to vomit up all of the Garmonbozia he had been collecting over the years.]]
* In 3.05, [[spoiler: the scene of BOB!Cooper looking in the mirror, and his face slowly morphing into BOB's. Subtle, and incredibly creepy.]]
* Doc Hayward recalls that one of the only things "Cooper" did before leaving Twin Peaks was visiting a comatose Audrey. Given that the same episode heavily implies [[spoiler: the Doppelganger raped Diane]] and there's a [[spoiler: new Horne running around who could definitely be 25]] the implications are very unpleasant...
** And if that is the case, it would certainly explain a lot of [[spoiler:Richard's behavior.]]
* Part 8. '''ALL OF IT.'''
-->'''The Woodsman:''' [[BrownNote This is the water and this is the well. Drink full and descend. The horse is the white of the eyes and dark within]].
* In Part 13, "Cooper" is forced into an arm wrestling match. While his victory is actually quite awesome, his taunts are rather unsettling, and makes his brutal murder of his opponent that much more disturbing.
--> '''Mr. C:''' See? Doesn't that hurt your arm when I go like that?
* [[spoiler:Sarah Palmer removing her face in Part 14, at first revealing a void and then a disturbing smile. This somehow causes the bar patron tormenting her to get his throat ripped out.]]