* In the first ''Survivor'', you learn from a file that Sheena Island, the mass production plant for "Mr. X"-style Tyrants, has achieved this role by making use of a hormone found inside of teenagers' brains, and it's produced when they're frightened/stressed. Umbrella knows maximum fright/stress levels are caused by excruciating pain. Yes, Umbrella tortures kids to death in order to further their goals. Just let that sink in.
** Worse, they open their skulls while '''alive and fully conscious''' in order to get said hormones.
* Vincent Goldman himself is a creepy, creepy monster far worse than any of the zombies in the game. He's so bad that the people under him were trying to get him ousted from Umbrella for the sick stuff he was doing by reporting to corporate headquarters, and the game indicates [[CorruptCorporateExecutive Umbrella]] ''[[EvenEvilHasStandards would have done it]]'' (or at the very least, Vincent took the threat of that happening seriously). This is the same group of people who [[NightmareFuel/ResidentEvilCodeVeronica gave Alfred Ashford his own private stock of prisoners to torture and execute]], and [[NightmareFuel/ResidentEvil2 gave the position of Raccoon City Police Chief to a psychotic rapist]].
** His diary is even worse, as it reveals that ''he released the T-Virus on the island'' '''on purpose''' ''just as a "Screw You" to the people on the island who wanted him gone''. Together with the other entries, it makes him seem even more evil than he actually looks.
* The Trashsweepers. Utilizing UncannyValley to the extreme, these beings look like GasMaskMooks... until they start moving in ways no normal person should move ever. And the hooting...
** How about the fact that the Trashsweepers are explicitly described as being one of the lines of B.O.Ws that Umbrella has created? A species of genetically engineered {{Super Soldier}}s who have enough intelligence to use pack tactics, operate firearms, and obey orders instead of randomly attacking anything around them. This makes them potentially one of the most dangerous B.O.Ws that Umbrella has created.
* The Hypnos Tyrant. It's first form is uncannily human, and is introduced by killing Vincent/Andy/the UT Commander. Its second and third forms are the scariest, however: with an inhuman grimace, monkey-like sounds, and the fact that it's always angry make it a terror to fight. Oh, [[ImplacableMan and it took two missiles from a helicopter to destroy the thing]].
* The diary entries in this game are DarkerAndEdgier than other entries in the series but two stand out:
** The first is from a prisoner who was kidnapped by Umbrella, and his discovery of their plans and attempt to escape with other kids. This is after you learn that Vincent gunned down escapees from the prison, and the last entry in the diary is the night before that happened.
*** Even worse, you find a bunch of bodies with their throats slit. [[NothingIsScarier It's never explained what happened to them or what did it.]]
** The second diary entry is from a worker there, who is a firm case of EvenEvilHasStandards (he wanted them to have anesthetics) and by the end of his entry states this chilling line:
-->-- ''I'm sure I'm going to the worst kind of Hell when I die. Or perhaps I'm already there.''