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[[caption-width-right:350: The massive amount of blood makes it even more horrific.]]
* Travis' MadnessMantra in his speech during the very first boss. "Can't find the exit. Can't find the exit. Can't find the exit. Can't find the exit. Can't find the exit." If you notice it, it very ominously foreshadows that this isn't just a wacky action game.
* #7, Destroyman, is very subtly unhinged. The cutscene proceeding his fight is rather creepy, as every time the camera zooms in for a closeup on him, a deafening DroneOfDread plays and the camera shakes about. It conveys that despite his casual demeanor, Destroyman is not a sane individual, especially considering how you confront him in what looks like a snuff film set.
** Even Travis is unsettled upon entering the set and seeing the bodies of random people Destroyman killed.
* When trying to get the location of the #5 assassin from Sylvia, Travis loses the connection and is left on his own. So how do you find out where to go? Well, you could either look at the minimap like usual or follow the ''freaking trail of blood'' that suddenly appears out of nowhere.
** And then you get to the end of the blood trail. There's a murdered woman's body lying in front of the spot. [[NothingIsScarier You never find out who she is, who killed her, or why]]. She's just ''there.'' Even though you don't actually fight Letz Shake it makes [[MoodWhiplash the atmosphere a lot chillier than before.]]
* There's also the time that the #4 assassin gets his eye sliced off, cries because he's scared of the dark, and his "assistants" tie him to a crucifix and cut him in half with a giant circular saw blade. And everyone starts applauding, despite the fact that no one is in there.
* The cutscene and fight for the #2 assassin, Bad Girl. Of course, most of the game's schtick is switching between lighthearted parody and Nightmare Fuel moments.
** While he's not one to talk, it's pretty easy to see why Travis is creeped out by Bad Girl, who he calls a "perverted killing machine."
** Her back story in ''Travis Strikes Again'', adding a new layer of creepy to her. Imagine learning your child was kidnapped from you and forced through brutal electric tortures meant to make her believe she's going to die. Imagine someone doing that repeatedly to them until they completely snap and become, as Travis put it in the first game, a perverted killing machine. Then you start receiving videos of them brutally murdering people in fights to the death, filmed and sent at your child's request, until one day, you watch the moment they meet someone they couldn't beat and you can't do anything but watch them die. That might be one of the most fucked up backstories to any character in media period.
* The final fight between Travis and Jeane is full of these moments.
** Dark Star, in an attempt to manipulate Travis makes him remember the day of his parents' deaths at the hands of Jeane. Their corpses lie in a pool of blood. His mother has a missing arm (which Jeane ripped out), her entrails spilled on the floor and her neck seemingly broken. His father isn't much better, with a caved-in head (with spilled brain mush) and a stomped-on leg. Jeane is standing behind them, holding Travis' mother's arm, drenched in blood and almost naked except for her underwear. Then Jeane glances towards Travis with a PsychoticSmirk. It is no wonder he [[TraumaInducedAmnesia forgot who killed them]].
** Then everything goes to hell when Jeane introduces herself, by punching through Dark Star's groin and immediately killing him. This finally leads Travis to remember why he became an assassin: To kill Jeane and avenge his family.
-->'''Jeane:''' You're just as gullible as ever, Travis. Why would he be your father?
** Jeane actually tells her backstory when Travis' fast-forward is slowed-down. She ends up revealing their father was a horrendous piece of work that enslaved her and even raped her, horrifying Travis in the process.
-->'''Jeane:''' OK, I'll make it short and quick: You are my half-brother. You know that manga called "Manga/{{Miyuki}}"? The Japanese one? Well, it's like that. Your father abandoned ''my'' mother, to run off with ''your'' mother. It broke her heart beyond repair. She killed herself. Knowing I had nowhere to go, he took advantage of me. Ever since I can remember, he molested me. We lived in this ''rotten'' apartment, and I was his slave. Every day I cursed his soul. I swore that I would kill him one day, but cursing didn't change anything. That's when I decided to become a killer. With no money to pay for training, I paid with my body. But you, ha, I bet that son of a bitch looked like a hard-working family man. ''That'' was all an act. He didn't give two shits about cleaning up my life... so I decided to clean it up myself. [[FreudianExcuse That's why I do what I do]], and I got what I wanted: Killing him in front of your very eyes. That's my story.
** Jeane has a monstrous strength and endurance. She's so strong she doesn't need a beam katana to brutalize Travis, fighting only with her fists and legs.
** The way their fight ends. Travis doesn't finish the battle with a Death Blow like he did with the others, ''Jeane'' does, and she punches right through his chest. If [[BigDamnHeroes Shinobu]] hadn't cut off her arm, Jeane would've killed him.
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