* The infamous scene featuring two guys, a diner, and [[spoiler:something resembling a zombie hobo out back]].
** Also a little meta-HarsherInHindsight. Remember when the one guy says "I hope I never see that face outside of a dream"? Flash forward 5 years to InlandEmpire and, well...we get to find out exactly how he was feeling.
* Sierra Boniokayta. This is when the film takes a much darker turn, as Betty and Rita investigate the apartment of Diane Selwyn, looking for answers regarding Rita's true identity. Do they find anything? Yes: [[spoiler:Diane's decaying corpse in a bed.]] The neighbor's knocking on the door somehow makes it worse.
* Much of the film's cinematography style and soundtrack.
* The old couple with the ghastly grins.
* ''[[https://www.youtube.com/watch?v=xrC3Bf-CvHU Llorando]]''. What starts as an ominous stage performance gradually shifts into a TearJerker, and somehow becomes more terrifying the longer it goes on. The film goes straight into MindScrew territory just after.