* Shira [[spoiler:torturing two Itto-ryu fighters and a prostitute from the Yukimachi]].
* Hyakurin's [[spoiler:torture and rape at the hands of the Itto-ryu]].
* Burando's experiments with the prisoners.
** Burando's ultimate creation, Nue #1, is a primitive cyborg--metal plates fused into his skin across the back of his neck to make him almost immune to decapitation, a metal grill covering his face, large twin blades [[BladeBelowTheShoulder bolted to each forearm]], an insane HealingFactor almost on par with Manji's thanks to the bloodworms infused in his body and ''utterly, psychotically insane!''
* Shira [[spoiler: tearing apart three Shogunate officers was bad enough, but the fourth one [[ColdBloodedTorture gets it]] [[RapeIsASpecialKindOfEvil the worst]]...]]
* The nightmarishly horrific MutualKill of Itto-Ryu warrior Kunimitsu Amon and Rokki-dan warrior Murasaki Shozo. When Amon forces his way into close range against Shozo, the midget torturer (who fights with [[HollywoodAcid highly corrosive acid]]) uses a hand-held pump to inject a large amount of acid directly into Amon's subtantial gut, almost melting him in half. With his dying breath, Amon grabs Shozo and [[TakingYouWithMe forces him face-first into his melting belly]], suffocating and burning him in turn until [[BodyHorror Shozo's half-melted skull emerges from Amon's disintegrating]] ''[[BodyHorror back!]]''