* While most ''RedVsBlue'' characters are some level of UnsympatheticComedyProtagonist, there are still a few Nice Guys.
** Donut on the Red Team is so perky, friendly, and sweet that even ''Church'' (who otherwise hates everyone) likes him. He's essentially the closest thing ''[=RvB=]'' has to TheCutie.
** Caboose ''seems'' to be vaguely aware that half the people he knows are supposed to be his enemies, but that doesn't mean they can't be his friends.
** Sheila is remarkably personable and helpful for literally being a tank, and is a {{Cute Machine|s}}.
** The Freelancers have their own sort of PowerTrio of Nice Guys in the form of Agents New York, North Dakota, and Washington. York is incredibly snarky, but has a strong sense of fair play and right and wrong and his snark is usually intended as good-natured. Wash is the dorkiest one, worried about following the rules and everyone getting along. Lastly, North is the TeamMom, so nurturing and caring that he was specifically given the most child-like of the AIs as a result.
** Finally there's Doc, who comes off as almost a parody of a Nice Guy. He's an ActualPacifist and has an exaggerated GranolaBoy and SoapboxSadie personality, but he's also snarky in a biting way and sometimes shows an extremely morbid and almost NightmareFetishist sense of humor.
* ''WebAnimation/{{RWBY}}'':
** Jaune Arc is the first person that Ruby befriends at Beacon Academy, the fact that she called him "Vomit Boy" rolling off his back.
** Pyrrha Nikos is polite, supporting of the other students ([[UndyingLoyalty especially Jaune]]), forgiving, and generally pleasant [[TheStoic if occasionally somewhat detached and professional]].
* Quite a few characters in ''SurvivalOfTheFittest'', but one character who plays this to a tee is Reika Ishida, in contrast to her [[PsychoLesbian sister]]. One of her defining traits was that she was nice and friendly, always trying to help out. However, true to the stereotype, she literally gets killed within two posts once she gets to the island. Danya in the following announcement then called her "the less interesting of the Ishida sisters" for this reason.
* ''WebVideo/DoctorHorriblesSingAlongBlog'':
** Penny is extremely sweet-natured, a good friend to Billy, vegetarian, and volunteers to help the homeless.
** Billy is also on the surface a nice guy, but he becomes more of a DoggedNiceGuy around her, and arguably he has some ideas that [[IneffectualSympatheticVillain maybe]] [[MadScientist aren't]] [[WellIntentionedExtremist so]] [[BewareTheNiceOnes nice]].
* FeliciaDay also plays Codex on ''TheGuild'', who is a {{Deconstruction}} of this trope. While she's generally very tolerant and patient with the rest of the group and serves as TheHeart, the constant pressures of being the WhiteMagicianGirl (in-game and out) have fed into her crippling neurosis.
* Personified with the "[[http://knowyourmeme.com/memes/Good-Guy-Greg Good Guy Greg]]" ImageMacro, who is the "nice guy" counterpart to [[{{Jerkass}} Scumbag Steve]].
* Creator/DougWalker's niceness was lampshaded and parodied in his cameo for WebVideo/{{Phelous}}'s review of ''Film/HouseOfWax2005''. While the rest of the site cheered for Paris Hilton's character's death, he's disgusted by their behaviour even though she's fictional.
* Speaking of TGWTG, the late and much-missed [[WebVideo/YouCanPlayThis JewWario]] (even in character, which was close to his real life persona ''anyway'') was probably THE nicest person out of the entire cast/crew.
* Tacoma from WebVideo/DemoReel is an always-willing [[TheConfidant confidant]] and LivingEmotionalCrutch for [[RapeAsBackstory Rebecca]] and [[ParentalAbandonment Donnie]]. And speaking of those two, while they have plenty more issues, they're sweethearts as well when you get to know them better.
* Rack of the WhateleyUniverse. He's a dwarf on top of being a mutant, but he has several extremely pretty female friends because he treats them well. And he makes the time to explain to Chaka why she should watch out for the dreaded Sahar.
* In ''Literature/{{Worm}}'', the superhero Legend, leader of the [[SuperTeam Protectorate]], inspires an almost universal "he's so nice!" reaction in people who meet him, and is generally considered the nicest member of the [[PowerTrio Triumvirate]], with Alexandria being cold and distant and nobody liking Eidolon. [[spoiler: As it turns out, he's also the only member of the Triumvirate that isn't aware of the horrible crimes perpetrated by Cauldron.]]
** There's also a twisted inversion of this trope in the form of a villain actually ''named'' "Nice Guy." He pretty much renders everyone around him incapable of regarding him as a threat, and most people actually trust him and think of him as a friend, allowing him to calmly walk up to people and start murdering them where they stand, with their friends blithely standing by and unable to do anything about it because he's such a ''nice guy''.
* ''WebVideo/TheAutobiographyOfJaneEyre'': PlayedWith a lot with people Jane encounters when she starts her new job.
** Grace Poole is a CompositeCharacter, combining mild and kind Mrs Fairfax and scary, mysterious Grace Poole from the book. She's very intense and weird during her first appearance. Later she has moments when she's genuinely nice to Jane and very caring. Other times she seems nervous, impatient or downright nasty.
** Jane expected Mr Rochester, her employer, to be horrible, based on Grace's vague description and Adele's [[NightmareFuelColoringBook nightmarish drawings]] of him. She finds out he's not abusive, which she thought he would be, but he's teasing, weird and bossy. In episode 13, Jane says that he seems a nice enough man, though a little negative, moody, sharp, dismissive,... but in a good way. There are obviously things which haunt him.
* Deckard in ''WebAnimation/BeeAndPuppycat'' makes Bee a casserole after hearing she lost her job. He even leaves it for Bee after she accidentally {{Groin Attack}}s him with her umbrella.
* Some human/sentient SCP items at the ''SCPFoundation'' are surprisingly polite and friendly, such [[http://www.scp-wiki.net/scp-073 SCP-073]], [[http://www.scp-wiki.net/scp-208 SCP-208]] and [[http://www.scp-wiki.net/scp-105 SCP-105]].
** [[http://www.scp-wiki.net/scp-999 SCP-999]] despite being a BlobMonster maybe the nicest creature the foundation has, [[FriendToAllLivingThings it loves all living creatures]] and just wants to hug and tickle people.
** Most of the [[CreepyChild child SCP items]], such as [[http://www.scp-wiki.net/scp-053 SCP-053]], [[http://www.scp-wiki.net/scp-239 SCP-239]] and [[http://www.scp-wiki.net/scp-040 SCP-040]], are genuinely nice kids, probably because ChildrenAreInnocent.
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