One character has recently lost their virginity (or a pair of characters has recently lost their virginity together). This fact is immediately obvious to yet another character (usually, but certainly not always, an older woman), despite there being absolutely no outward sign or obvious reason to think that. The characters aren't acting cute together, there's no tell-tale MessyHair or [[SexDressed mussed clothing]], nor even the subtle aroma of "just had sex".
The other character merely looks upon the new non-virgin, as if using Virgin Vision as a superpower, and they know. (WomensMysteries, perhaps.)
Compare to DidYouJustHaveSex (where other people know because the characters are giving away clues) and VirginityFlag (where there is some obvious means of telling who is and is not a virgin).
[[folder: Anime & Manga ]]
* ''Manga/FutariEcchi'' has Rika have the ability to tell whether someone is a virgin or isn't, just by looking at them. She mentions that she knew that Shouko, [[OlderThanTheyLook who is 24 years old, despite looking half her age]], was not a virgin and flat-out points at [[ChristmasCake Kyoko]] that she had finally lost her virginity, much to Kyoko's surprise and embarrassment.
[[folder: Comics ]]
* As an inversion, in the [[FrancoBelgianComics French comic]] ''Le Chant d'Excalibur'', '''everybody''' can apparently detect that the heroine is still a pucelle, and will mention it at the first occasion.
[[folder: Fanfiction ]]
* Duncan [=McSmurf=] is able to tell if a fellow Smurf had "[[NocturnalEmission the Smurfette dream]]" just by smell alone in the ''Fanfic/EmpathTheLuckiestSmurf'' story "I Dream Of Smurfette".
[[folder: Film ]]
* In ''Film/ShakespeareInLove'', Queen Elizabeth can tell that Viola has been "[[DoubleEntendre plucked]] since [she] saw her last." The Queen adds that "it takes a woman to know it," though she doesn't reveal how, exactly.
* This happens to the main character in ''Film/RealWomenHaveCurves''. Her mom figures it while watching Ana stand in front of a full-length mirror.
* In the ''Film/WestSideStory'' movie, Anita doesn't say it out loud, but she gives Maria this look that says it all.
* The second ''Film/AceVentura'' film:
-->"She's not a virgin!"
-->"They can tell that?"
-->(Cue being ChasedByAngryNatives)
* In ''Film/SayAnything'', Lloyd Dobbler's friends can tell that he and Diane Court "did the deed", despite Lloyd's "I admit nothing" disclaimers.
* Inverted in ''Film/TheFortyYearOldVirgin'', where the 16 year old daughter of the main character's romantic interest can tell he's lying about having had sex and is still a virgin. When he asks how she can tell, all she says is that she goes to school with tons of boys and can somehow "just tell" who's had sex and who hasn't.
[[folder: Literature ]]
* In the ''Literature/MorganvilleVampires'', Claire fears this trope. Sure enough, Eve barely looks up and realizes what happened.
* In ''Literature/TheSoundOfWaves'' by Creator/YukioMishima, older women claim to be able to determine whether a girl is a virgin just by looking at her breasts.
* In ''Literature/TheDresdenFiles'' series, [[HornyDevils White Court vampires]] can sense virginity, which led to a CrowningMomentOfFunny when Lara Raith (leader of the White Court vamps) met Carlos Ramirez (a CasanovaWannabe).
** [[JustifiedTrope Justified;]] they feed on energy, [[HornyDevils most particularly sexual energy.]]
* The first chapter of Alina Adams figure skating mystery ''Death Drop'' starts with the researcher heroine listening to commentator insisting "You can always tell a virgin by the way she skates." She thinks the non-virgins skate better. She turns out to be right in her assessment of the skater they were watching.
* In ''Blue Smoke'' by Creator/NoraRoberts, Reena's mother is able to detect instantly that her daughter has lost her virginity when Reena arrives home after doing the deed. The scene is retained in the made-for-TV movie based on the book.
* In ''Literature/FiftyShadesOfGrey'', Kate could just ''tell'' that Ana slept with Christian.
* Not a virgin example in ''Literature/TheLostFleet: Dauntless'', but the first time Captain John Geary has sex after his 100-year stint as a HumanPopsicle, the titular ship's captain, a young woman, immediately knows it, even though he's doing his best to act normally. When asking his sexual partner, she replies that this is a "need-to-know" kind of information. He interprets it as WomensMysteries. To be fair, though, he does admit to himself that this is the first time he felt himself without "ice" inside him, so it's likely he ''was'' giving off subtle signals.
[[folder: Live Action TV ]]
* After Donna and Eric ''[[AllWomenArePrudes finally]]'' have sex in the third season of ''Series/That70sShow'', neither one admits anything to the other characters ([[AllMenArePerverts despite Eric really wanting to do so]]). When Donna goes to talk to Jackie, Jackie see her as having the words "I Had Sex" written on her forehead, and Jackie immediately rushes her away so they can talk about it. When Eric goes to talk to Bob, Bob sees Eric as having the word "Guilty" on his forehead, inidcating that Bob is suspicious, but Bob makes no sign of knowing what happened until he officially finds out a few episodes later.
* In the ''Series/BuffyTheVampireSlayer'' episode "Innocence," Buffy's mom can tell there's ''something'' different about her daughter, but not what. Buffy ''was'' acting as if she had something to hide, [[TheMasquerade but that's hardly unusual for her]].
* There's a very similar scene in ''Series/VeronicaMars''. When Veronica comes back from sleeping with Duncan, Keith looks closely at her and says that she seems different.
* On ''Series/TheFreshPrinceOfBelAir'' when Carlton loses his virginity, he discusses it with Will using code words because Ashley is in the room. When they leave and Hilary asks what's up, Ashley replies "Carlton lost his virginity."
* In ''Series/XenaWarriorPrincess'':
-->Guard, pulling Gabrielle aside: "Virgins only."
-->Gabrielle: "What, is it written on my forehead?!"
[[folder: Video Games ]]
* Oghren in ''VideoGame/DragonAgeOrigins'' claims he can "smell purity a mile away". Apparently it's not a very useful skill. "Be much better if I could smell cheese."
* In ''VideoGame/TheWitcher3WildHunt'', an old blind woman sniffs Ciri, declaring her a virgin and allowed to proceed to meet the [[WickedWitch Crones]].
[[folder: Web Comics ]]
* Combined with AuraVision, it ''is'' a superpower in the ''Webcomic/CiemWebcomicSeries''. Leaves the heroine feeling DefiledForever, though she does adjust to it eventually.