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[[quoteright:300:[[VisualNovel/TokimekiMemorial http://static.tvtropes.org/pmwiki/pub/images/tokimemo2_shirayuki_twins2a_9195.png]]]]
[[caption-width-right:300:The Shirayuki sisters: a sweet [[TheIngenue Ingenue]] on the left, a [[GenkiGirl mischievous]] blunt girl on the right. They also disagree on [[GirlWithPsychoWeapon the way of keeping]] their TwinSwitch game secret.]]

->'''Skeeter''': He's real brainy.\\
'''Scooter''': She's athletic.\\
'''Skeeter''': We're so different...\\
'''Scooter''': It's pathetic.\\
She does stunts and\\
I assist her\\
'''Skeeter''': He's my brother!\\
'''Scooter''': And they tell me she's my sister!
-->-- ''WesternAnimation/MuppetBabies''

A SubTrope of the OddCouple wherein the odd couple are twins, usually identical brothers or sisters. A prominent exception is actually in the former {{Trope Namer|s}}, ''Series/ThePattyDukeShow''. It featured identical ''cousins'', who were described as "One pair of matching bookends, different as night and day" in the ExpositoryThemeTune.

Basically, SiblingYinYang for ([[AlwaysIdenticalTwins usually identical]]) twins. They'll usually embrace using {{Identical Twin ID Tag}}s to help the differentiation.

Sometimes overlaps with CainAndAbel, in which case you might expect FearfulSymmetry. Also frequently overlaps with FoolishSiblingResponsibleSibling and RedOniBlueOni.

Compare EvilTwin. Extreme opposite is SingleMindedTwins or CreepyTwins.

[[folder:Anime and Manga]]
%%* ''{{Manga/ARAGO}}'': Arago and Ewan fit this trope to a T.
* Tsubasa and Arisa from ''Manga/{{Arisa}}''. [[TheAce Arisa]] is loved by everyone at her school, has good grades and is the ClassRepresentative. [[{{Delinquent}} Tsubasa]] is nicknamed "the Demon Princess" and is constantly getting into fights and has lousy grades. [[spoiler:As it turns out, Arisa is very much a BrokenAce]].
* In ''Manga/{{Aruosumente}}'', the twins Lante and Dante. Lante is cheerful, outgoing, chatty, frilly and can't fight to safe his life. Dante, on the other hand, barely talks at all, keeps to himself, cares only about Lante and is the best fighter in the kingdom, to the point of being called a beast. Interestingly, it's Lante who may be a schemer and capable liar, while Dante is very straighforward.
* ''WebComic/AxisPowersHetalia'': the BoisterousBruiser America and the ShrinkingViolet Canada are only implied to be twins, but they fit in fairly well. The show also has sweet, softhearted [[LovableCoward Italy]] Veneziano and his brother, the temperamental, hotheaded [[{{Tsundere}} Italy]] Romano.
* ''LightNovel/BakaAndTestSummonTheBeasts'': the NiceGuy Hideyoshi and the {{Jerkass}} Yuuko. Further differences include that Hideyoshi is not smart enough to qualify past Class F (the bottom of the barrel), while Yuuko is in Class A (the best), and Hideyoshi is a beautiful singer but Yuuko is completely tone-deaf.
* ''Manga/{{Bleach}}'': The sweet HouseWife-in-training[[note]]She's still a kid, after all.[[/note]] Yuzu and the rough {{Tomboy}} Karin.
* ''Manga/BlueExorcist'' is essentially about a pair of these, as both Okumura brothers are very different: [[BigLittleBrother Yukio]] is a [[TheStoic stoic]] BadassBookworm TeenGenius who subverts HalfHumanHybrid due to being sickly when young, while [[AntiAntiChrist Rin]] is a HotBlooded BookDumb AlmightyJanitor who is the inheritor of {{Satan}}'s power. They fell into this when younger as well, with [[IllGirl Yukio]] being a ShrinkingViolet CuteBookworm (and still has shades of both still), while [[BigBrotherInstinct Rin]] was a PintSizedPowerhouse {{loner|sAreFreaks}}.
%%* In ''Manga/ChronoCrusade'' we have [[spoiler:Chrono and his EvilTwin Aion]].
* ''Anime/DigimonFrontier'': Kouji and Koichi. Kouji is arrogant and kind of a jerk at first, [[IWorkAlone working by himself]] because he thinks the others will only drag him down. As time goes on, he shows a much kinder side. His Digimon forms are wolf-based and his element is light. Meanwhile, his twin is similar in appearance but far more soft-spoken and willing to be in the group [[spoiler:once he's de-brainwashed]]. And, his Digimon forms are lion-based and his element is darkness (DarkIsNotEvil).
* ''Manga/DNAngel'': Riku and Risa even come out and say that their personalities are completely different. Risa is very girly and rather shallow ([[HiddenDepths most of the time]]), while Riku is tomboy-ish and more down to earth.
* ''{{LightNovel/Durarara}}'': Mairu and Kururi Orihara actually [[InvokedTrope invoked]] this. They actually ''flipped a coin'' to decide which girl got which trait. Strangely enough, their roles (Mairu as the GenkiGirl and Kururi as TheQuietOne) switch when they're on the internet. Even weirder is the fact that they don't consider themselves [[TheDividual separate people]].
* ''Manga/{{Eyeshield 21}}'': The Kongo twins Unsui and Agon. Unsui has a shaved head like a monk (it's sort of a theme at their school) is intensely hard working, diligent, cautious, a very good athlete and basically a decent guy. But Agon (with a distinctive dreadlock hairstyle) is the series' {{Jerkass}}, a JerkJock to the extreme (including the womanizing, which apparently started in middle school) who gets away with being a sociopathic thug, slacker and general all-around asshole because he is [[TheGift a talent seen only once in a hundred years.]] It emphasized by the fact that 'A' and 'Un' represent "beginning and end" aka birth and death.
* ''Manga/FushigiYuugi'' gives us easygoing, kind, polite Amiboshi contrasted to HotBlooded {{Jerkass}} Suboshi. Heck, even their weapons are different: Amiboshi has a flute which he can use for MindControl on his opponents (for good or evil purposes), and Suboshi prefers to just smash through everything with a KillerYoYo. Even their clothes reflect just how opposite they are: Amiboshi wears a blue tunic, and Suboshi wears an orange one. (At least in the series; in promo artwork, they are very frequently dressed ''exactly'' alike.)
* Takuma and Kazuma from ''Manga/GakuenBabysitters''. The former is perpetually cheerful, and the latter is perpetually teary-eyed.
* Although the later being conceived in a [[LiteralSplitPersonality less]]-[[EnemyWithout than]]-[[EvilTwin natural]] fashion, [[spoiler:Amata Sora and Kagura Demuri]] from ''Anime/AquarionEvol'' fit this to a T. [[spoiler:Kagura]] is a [[FieryRedhead hotblooded]] [[BloodKnight warrior]] from the enemy land raised to be a savage [[AttackAttackAttack killing machine]], while [[spoiler:Amata]] is an [[ButtMonkey insecure]], [[FriendlessBackground shy]] and [[NiceGuy gentle guy]] with issues [[FreudianExcuse regarding his mother's apparent abandonment when he was a kid]]. [[CockFight Them being opposites of each other is actually a plot point.]]
* ''Anime/GlassFleet'''s [[spoiler:Vetti and Cleo]] exemplify this trope. Though, their personalities are passed off as them having been raised separately, with the younger one subjected to [[spoiler:mental and sexual abuse]], giving him his screwed up personality.
* ''Manga/HekikaiNoAion'': The twin sisters Shizuki and Yuzuki. Shizuki is a {{Manipulative B|astard}}itch and an IllGirl, in the other hand, Yuzuki is a [[TheDitz Ditz]] and a DumbMuscle.
* ''Anime/HighSchoolStarMusical'' has two pairs - Nayuki's younger sisters, Tsumugi and Yuki, are more typical and aren't major characters. But separated twins [[spoiler:Tsubasa and Itsuki]] - who aren't even known to be related - have a large part of their conflict revolve around how they've developed into this since being separated.
* ''Anime/ImGonnaBeAnAngel'': Noelle (the light) and Silky (the darkness) [[spoiler:as described by Mikael. Mikael and Raphael might also count.]]
* The Fubuki brothers in ''VideoGame/InazumaEleven''. Shirou is a gentle, calm and timid defender, while Atsuya is a HotBlooded and [[{{Jerkass}} rude]] forward. Coincidentally, [[RedOniBlueOni Shirou's hair is bluish-gray and Atsuya' s hair is pink]].
* ''Kiss x Sis'': We have Emotional Red Oni Ako and Soft-Spoken Blue Oni Riko.
* ''Anime/LuckyStar'': Kagami and Tsukasa. The former is a somber {{Tsundere}} while the other is a [[TheDitz ditzy]] {{Cloudcuckoolander}}. When asked about this by Konata, they point out that they are fraternal twins.
* Fuka and Fumika Narutaki of ''Manga/MahouSenseiNegima''. The former's the trouble-making class pankster while the latter's constantly worried about how the former's pranks will get them in trouble as she gets dragged along.
* In ''Manga/MiracleGirls'', Tomomi is an outgoing and athletic tomboy while Mikage is feminine, studious, intellectual, and bad at sports.
* ''[[Anime/MobileSuitGundamSEED Gundam SEED]]'' has [[spoiler:Kira and Cagalli]]. The former is quiet, polite, and kind to a fault. The latter is brash, flippant, and has quite a temper. Also one's a boy and one's a girl. [[spoiler:And one is genetically engineered to be physically "perfect", while the other...has superior physical abilities without any genetic alteration.]]
* ''{{Manga/Monster}}'': Anna and Johan Liebert. Anna's a nice, sweet girl who practices aikido and delivers pizza as a part-time job. Johan is the [[ExactlyWhatItSaysOnTheTin title character]].
* Yuma and Yuna Kashiwagi from ''Anime/PaniPoniDash''. Yuma is an extreme extrovert, Yuna is an extreme introvert. Yuma goes so far as to actively sabotage Yuna's attempts at being more accepted just to keep eyes on her.
* ''Manga/PandoraHearts'' has Alice and the Will of the Abyss. Alice is boisterous, tomboyish, ill-mannered and she likes to wear dark dresses as opposed to her sister, who is lady-like in every way and always wears light-colored dresses. [[spoiler:Their opposite personalities, tastes and clothing are what makes obvious which twin was in control of Alice's body in the flashbacks.]]
* ''Pokemon Omega Ruby Crimson Passion & Pokemon Alpha Sapphire Indigo Wisdom'' has twins Alpha and Omega. Omega is a calm boy with blue TsurimeEyes while his older brother Alpha is a hot-headed goofball with red TaremeEyes.
* ''Manga/RanmaOneHalf'': Towards the end of the manga, there is Pink and Link, identical twin girls from a village of herbalists that neighbours Shampoo's village of "Chinese Amazons" and who practice different branches of an unnamed [[MartialArtsAndCrafts "Martial Arts Herbalism"]], a fighting style that revolves around plants and herbalistic concoctions. Pink, who is usually smiling, is a wicked and vicious girl who loves to poison people. Link, who is usually frowning, is a kind and gentle girl who considers it her duty to heal those her sister hurts. Despite these opposite natures, they care for each other deeply and are capable of uniting with singular purpose when given motive... which means that, in their actual appearance in Nerima, they function more like SingleMindedTwins.
* Suiseiseki and Souseiseki from ''Manga/RozenMaiden'' are very much this, both including a TomboyAndGirlyGirl relationship, but with several more differing characteristic traits. Where Souseiseki is {{stoic}}, Suiseiseki is [[TheMcCoy emotional]]. Souseiseki is serious and collected, while Suiseiseki is [[ScrewySquirrel mischievous]]. Souseiseki believes it is the duty for every doll to participate in the Alice Game regardless of personal attachments, while Suiseiseki strictly opposes the Alice Game due to fear of losing her sisters.
* ''Anime/SpiritedAway'': The identical twin sisters Yubaba and Zeniba. Yubaba is selfish and materialistic; Zeniba, while vengeful at first, turns out to be kindly and humble.
** It's worth noting, though, that the sisters aren't ''really'' polar opposites--Yubaba has an introspective side (musing about the nature of humanity and its greed), treats her workers relatively well, so long as they work hard, and genuinely loves her son Boh to the point of spoiling him. Similarly, Zeniba can be just as nasty as her sister--one of her first lines to Chihiro is a threat to rip the girl's tongue out if she says anything about her presence. Miyazaki seems to be [[DeconstructedTrope deconstructing]] this trope with the characters, pointing out that unlike fairy tale villains, most adults are both bad and good, with positive and negative qualities alike (a fact suggested by Chihiro eventually calling ''both'' sisters "Granny").
* ''Manga/PrivateActress'' has the twins Shuuto and Yuuto, Shiho's clients in one chapter. Older twin Shuuto is a MrFanservice and a famous model, while younger twin Yuuto is just as good looking but a ''very'' awkward FragileFlower. Shuuto, then, hires Shiho to pose as Yuuto's suitor, in an attempt to help him. [[spoiler:Massively subverted: Shiho eventually finds out that Shuuto and Yuuto are ''the same person'' and a case of ''SplitPersonality''.]]
* ''Manga/SaintSeiya'' has the twin brothers Saga (thoughtful, wise, just [[spoiler:until his SuperpoweredEvilSide kicks in]]) and Kanon (selfish, scheming, arrogant [[spoiler:until he has a HeelFaceTurn.]]) For major irony, Saga is the ''Gemini'' Saint, [[spoiler:with Kanon inheriting his position once Saga dies]].
** From the villainous side, the twins gods Hypnos and Thanatos. Sure, they are CreepyTwins on their own, and aside from acting similarly , they also have similar creepy eyes. However, most of the times, they are more of this trope because of their personality, as they are textbook examples of RedOniBlueOni with Thanatos as the red oni and Hypnos as the blue oni. Thanatos have much shorter fuse than Hypnos ever had, does show more emotion that Hypnos ever does, will jump into the fight if challenged, and underestimated humans in general. Hypnos however, hates fighting, and will seek ways to avoid fighting, and calmly analyzes his enemies. This is even more prominent in ''Manga/SaintSeiyaTheLostCanvas'', where they still share similar personality with their classical counterpart, where when they are defeated and sealed, Thanatos continue cursed Pope Sage pettily and furiously , when Hypnos [[GracefulLoser accepted his loss calmly and congratulates Hakurei instead. ]]
* ''Manga/SaintSeiyaTheLostCanvas'' continues this tradition with [[ItsAllAboutMe arrogant, narcissist, megalomaniac, self-centered]], scheming, sleek and dainty Aspros, with the gritty and [[LoveMartyr self-sacrificial]] Defteros. And yes, they are the Gemini Saints of their era, [[spoiler:where Defteros had to kill Aspros because he showed his true colors, wanting to kill Defteros so that he could be the Pope. After Aspros'death, Defteros takes the Gemini Cloth with him and becomes the new Gemini Saint]]
* In ''Anime/SaintSeiyaOmega'' we have Gemini Paradox who has a crazy split-personality and good, loving, kind Gemini Integra. [[spoiler:Same thing happens. Paradox is beaten by Ryuuho, and Integra picks up the Gemini Cloth afterwards during the Pallas War.]]
* ''Manga/SamuraiHighSchool'': Sakakido Kou and his sister Sakakido Tsukiko are MasculineGirlFeminineBoy.
* Creator/RumikoTakahashi's one-shot ''The Samurai'' has identical twin ninja sisters, one of whom is [[LovableSexManiac a total nymphomaniac]], while the other DoesNotLikeMen and HatesBeingTouched. Woe be unto the person who confuses the latter for the former.
* ''Manga/ThereBeyondTheBeyond'': gives us twin boys both named Virid Visette Viridian, who are summed up quite nicely by their titles: [[AxeCrazy "The Mad Prince"]] and [[ManipulativeBastard "The Sane Prince"]].
* ''Manga/ToLoveRu'' features Momo and Nana Deviluke, which also personify this trope very well. Momo is a {{Yandere}} {{Chessmaster}} sex maniac and can talk with plants. Nana is a B-Type {{Tsundere}} [[TheIngenue Ingenue]] prude and can talk with animals.
* ''Manga/{{Trigun}}'': Vash and Knives. One is a genocidal maniac and the other is a pacifist. [[CaptainObvious That's pretty different]]. Vash is also all about how other people feel and love and peace, and Knives doesn't even do empathy for his twin with whom he has an ''effing psychic link''. Vash acts like a fool whenever he can and Knives is all superiority and icy control except when his temper gets away from him. They do have a bit in common still, when you get past the good and evil divide. For instance, scary if you get them mad. Stubborn as hell. Charisma. Way of speaking, when serious, is even a little similar. Pretty good, when you consider they've been separated over a hundred times as long as they were together. When they were kids in the anime, one was unusually mature and one was goofy. No prizes for which. In the manga, they were much more similar, but Vash was more outgoing and [[UsedToBeASweetKid Knives was more sensitive]].
* Hamel and Sizer from ''Manga/ViolinistOfHameln''. Hamel is loud, obnoxious and tends to spaz out while his sister Sizer is calm, sensible and serious. He's arrogant and loves to boast while she's a bit shy and doesn't like a lot of attention. Them being opposites is also emphasized by the fact Hamel is half demon while Sizer is half angel, and she starts as a villain while Hamel is the hero.
* ''Franchise/YuGiOh'':
** Rua and Ruka (Leo and Luna, respectively, in the dub) from ''Anime/YuGiOh5Ds''. Rua is a rowdy, cocky duelist who overestimates himself. Ruka is quiet, introverted and is usually the one to notify Rua when he goes too far.
** The original series' Yugi and Yami Yugi can be counted as this if you consider them twins (of the split personality/alternate guy living in your head kind), with a dash of SiblingYinYang.
** the Tenma twins from the spin-off from the original series, Yu-Gi-Oh R. Gekkou is mature, gentle,reasonable and already independent from Pegasus, meanwhile Yakou is very dependent on Pegasus, that he flips his shit upon Pegasus's disappearance and takes over Kaiba Corporation to resurrect Pegasus, effectively becoming the BigBad, spends his time on screen showing his insanity,[[UnsportsmanlikeGloating gloating off his victory]] and telling the protagonists he is unstoppable, or ranting about the fact that Gekkou used to be AlwaysSomeoneBetter to him. In their flashback too, they are this. Gekkou is cheerful and always in the spotlight, meanwhile Yakou is gloomy and forgotten or only seen as the inferior version of Gekkou. [[ThemeTwinNaming Their names reflect this too.]] Gekkou means "Moon Verse" when Yakou means "Night Verse". Lampshaded by Yakou himself in his match against Yugi.
* Michele and Sara Crispino from ''Anime/YuriOnIce''. Michele is short-tempered and uptight, while Sara is friendly and more down-to-earth.

[[folder:Comic Books]]
* Jezebel and Dinah in ''ComicBook/{{Blacksad}}'': Dinah is a black bear (= dark brown) with a white patch on her chest, Jezebel a polar bear (= white) with a dark patch on her chest. Otherwise, they share face structure, and body and hair type. Personality-wise, Dinah is softer and Jezebel harder, although both have it in them to do what they need, if it's for righteous revenge.
* Ember and Suntop in ''ComicBook/ElfQuest''. She is a [[FieryRedhead fiesty, red-headed]] partially wolf-blooded mortal Wolfrider who is destined to become the next Chief of the Wolfriders. He is the complacant, magical, blonde, immortal grandson of the mystic leader of the Sun Village and the apprentice of the equally mystic Mother of Memory. WordOfGod is that they actually chose these different destinies while in the womb.
* ''ComicBook/TheFlash'':
** [[AttentionDeficitOohShiny Hyperactive]], FunPersonified speedster Bart Allen (aka (Impulse) and his collected, scheming, evil clone Thaddeus Thawne (Inertia). Bart even mentions ''The Patty Duke Show'' the first time they meet, suggesting they [[TwinSwitch switch places to befuddle their friends]]. Thad responds by [[KickTheDog punching Bart in the nose]].
** The Tornado Twins, Don and Dawn Allen, are barely shown enough to have characterization other than as SingleMindedTwins, but their individual personalities are AllThereInTheManual: Don is studious, levelheaded, and prefers to carefully think of a plan before taking action, while Dawn is extroverted, impulsive and will follow her gut nine times out of ten.
* Gregory Stark and Tony Stark in [[ComicBook/UltimateMarvel Ultimate Avengers]]. [[spoiler:Becomes a case of CainAndAbel.]]
* In ''ComicBook/XMen Noir'', [[spoiler:Thomas and Robert Halloway]] are, respectively, a know-it-all afflicted with ChronicHeroSyndrome and a crude, violent sadist. It's probably important to note that they were adopted by different families after their father died.
* ''ComicBook/YoungAvengers'': Caring geek Wiccan and hyperactive badboy Speed.
* ''[[ComicBook/{{Swordquest}} Swordquest: Earthworld]]'' has Gemini, who is composed of an evil white shade and a helpful black shadow.
* In ''Comicbook/SilverSurfer'': Dawn Greenwood's EstablishingCharacterMoment is when she and her sister Eve see a shooting star (actually the Surfer), Eve wishes to go everywhere and see everything, and Dawn wishes that the star could keep going so everyone could get a wish. Eve thinks Dawn's wasted her wish since she didn't really ask for anything, and Dawn doesn't understand why anyone would want to leave their home town. As an adult Eve's been all over the world, and Dawn still doesn't see why anyone would leave. (Too bad [[TheCallKnowsWhereYouLive The Call Knows Where She Lives]].)
* ''ComicBook/TheAvengers'' Scarlet Witch and Quicksilver are polar opposites most of the time--Pietro is stern and stubborn and hot-tempered, his sister is gentle and patient and demure. This is especially true of their earliest appearances, where it was easier to define Wanda's personality as "Pietro's opposite". Over the years, however, Wanda has gained a stubborn temper of her own, making her more similar to her brother.

[[folder:Fan Fiction]]
* In ''FanFic/BoyScoutsOneHalf'', the Porter twins. Kirstin is a gentle, sweet homemaker. Nicole is a greedy, ruthless money seeker.
* In ''Fanfic/EmpathTheLuckiestSmurf'', Hefty and Handy are the BrainsAndBrawn twins, but are not identical.
* ''Fanfic/MoonSoldier'' features ditzy and emotional Usagi and her twin brother, the calmer and more responsible Akira.
* In Fanfic/TheRabbitAndThePuppy Kagami and Tsukasa from ''Manga/LuckyStar''. Kagami is a {{Tsundere}} and Tsukasa is {{TheDitz}}.
* ''Fanfic/MegsFamilySeries'' has Chris and Jillian's twins, C.J. and Tilly. The former basically inheriting their parents' (Lack of) brains, and the latter being a super-genius.
* ''[[http://archiveofourown.org/works/8491420/chapters/19459867 We are the Chatroom Gems]]'' has Yella and Azura Diamond, this story's versions of a human Yellow and Blue Diamond. Yella is cold, yells at a student (Lapis) for wearing a "Say hi if you're Bi" shirt, and is heavily implied to have undiagnosed IED, while Azura is emotional, has no problem with student's sexualities, and says that her depression is "apparently" obvious.

* ''Film/{{Eurotrip}}'': The twins Jenny and Jamie are pretty different. Throughout the movie the phrase "worst twins ever" is repeated.
* ''Film/HellboyIITheGoldenArmy'' has Prince Nuada and Princess Nuala; while they are HalfIdenticalTwins in appearance, WordOfGod says they were intended to be as different as night and day in all other respects.
* ''Film/ShesTheMan'': Sebastian and Viola. He's artsy and musical, and she's athletic and loves soccer. It's even lampshaded near the end of the movie.
* TheReveal in ''Film/ShockTreatment'' is the fact that this is the case of [[spoiler:Brad Majors and [[BigBad Farley Flavors]]]], who were seperated as infants when their parents died.
* ''Franchise/StarWars'': Luke is a blonde, blue-eyed, calm, mild-mannered, naive, mystical, religious Jedi Knight. Leia is a brunette, brown-eyed, quick-tempered, sharp-tongued, worldly-wise, shrewd, practical politician. Interestingly, [[GenerationXerox they parallel their parents' personalities from the Prequel Trilogy, with Luke taking after their mother Padme, while Leia takes after their father Anakin]].
** ''Franchise/StarWarsLegends'' shows Luke having trouble teaching Leia to be a Jedi. What worked for him (he got a lot of Jedi qualities) won't work for her. Hot temper is not something a Jedi knight ought to have. Especially since she's pregnant with Han's baby while trying to learn.
* ''Film/TheParentTrap'': In the 1998 remake, the twin girls, Annie James and Hallie Parker (Creator/LindsayLohan), are completely different. Annie is calm, reserved, and rather uptight while Hallie is relaxed, loud, outgoing, and a BigEater. (Compare Annie with Cody and Hallie with Zack of [[Series/TheSuiteLifeOfZackAndCody The Suite Life]].)
** Susan and Sharon in the original, to a lesser extent.
* Markus and William Corvinus, from ''Film/UnderworldEvolution'', were twin brothers who respectively became the first vampire and first werewolf. One is scheming and pompous, the other is near-mindless and savage. Unusual in that we never learn if they'd been genetically identical or not, as William is already locked permanently in his wolf-man form at the time the film is set, [[AlwaysIdenticalTwins but they probably were]].
* Rock star wannabe Roxy and [[BlondeRepublicanSexKitten Young Republican]] Jane in ''Film/NewYorkMinute''.
* Charlie and Donald Kaufman in ''Film/{{Adaptation}}'' have completely opposite approaches to screenwriting and to life.
* In ''Film/DoubleTrouble'', David is an honest cop with slobby habits, and his brother Peter is an unrepentant cat burglar with a taste for culture.
* The whole concept of ''Film/{{Twins}}'' is that Arnold Schwarzenegger and Danny Devito play genetically-engineered twins, with Arnold being the product of attempting to make the perfect man and Devito being all the "genetic garbage" they were weeding out of Arnold.
* In ''Film/ThePrettyOne'', Laurel and Audrey are identical twins. Audrey is self-assured, confident, and popular. Laurel is reclusive and is convinced that she is the inferior of the two.
* ''{{Film/Constantine}}'' has the aggressive cop Angela, and her twin sister Isabelle who is TheOphelia.
* ''WesternAnimation/QuestForCamelot'' has the serious and snooty Devon and the boisterous, fun-loving Cornwall. Unfortunately, they're not only Polar-Opposite Twins, they're also ConjoinedTwins, and they're unable to use their shared body to fly or breathe fire until they learn to get along near the end of the movie.

* Polgara and her twin Beldaran in ''Literature/TheBelgariad'' series. The former is dark-haired, has a grim disposition, and grew up to be an immortal sorceress. The latter was blonde, had a sunny disposition, and married young to become the queen of an island kingdom and bear the royal line which would deliver the child of prophecy, before dying young of pneumonia. As Polgara's biography explains, the two were originally much more similar, but their mother tampered with them in the womb to prepare them for their destinies.
* Occurs in Creator/GordonRDickson's ''Literature/ChildeCycle'' series of novels and short stories, in the characters of Ian and Kensie Graeme. Both are Dorsai ({{Proud Warrior Race Guy}}s). Ian is the epitome of the Warrior - seemingly aloof and intimidating by his sheer presence. Kensie is his polar opposite - warm, caring, social - everything that Ian is not. In the stories it's implied that together the two make up one individual. Ironically, it's Ian who's irresistible to women - ''[[ChickMagnet without realizing it]]''.
* ''Double Trouble'': Randi likes red, soccer, and is outgoing; Sandi likes pink and purple, schoolwork, and is rather quiet. They do agree that their two-year-old brother is a pill.
* The Brothers Majere; easy going, heroic BoisterousBruiser Caramon and intense, morally ambiguous SquishyWizard Raistlin in ''Literature/{{Dragonlance}}''.
* Lampshaded in ''Literature/TheLegendsongSaga''. Glynn and Ember are twins who are both physical and personality opposites. Their only physical similarity is the same colour eyes (yellow); personality contrasts include Ember�s musical talents and Glynn being tone-deaf.
* In ''Literature/DragonBones'', Erdrick is the shy, sensible twin, Beckram is a ladies' man and troublemaker. They look so alike that Beckram often persuades Erdrick to take his place at boring social occasions.
* Shaun and Shem from ''Literature/FinnegansWake'' probably count, but it's hard to figure out which is which, due to the book changing their names constantly. All you need to know is one is boring and dull, and the other is really creative and artistic.
** Specifically, Shaun is dull and conformist but successful in society, while Shem is a slovenly outcast but also an artistic genius.
* Owen and Porter from ''First Person Plural'' by Andrew Beierle. Owen is a book-reading drama nerd. Porter is a cheerleader-dating football player. Owen is gay, and Porter is straight. They are also conjoined twins sharing one body with two heads. This is incredibly awkward considering that Porter married a homophobic woman.
* Nicky and Alex in the ''Series/FullHouse'' book series grew up to be different, one tough, one shy, although in one episode of the show their father was concerned that their personalities would turn out the other way around.
* The Eiran twins Eamonn and Grainne from ''[[Literature/KushielsLegacy Kushiel's Dart]]'' by Jacqueline Carey.
* The Marlow twins Nicola and Lawrie from [[Literature/TheMarlowSeries the Marlow series]] by Antonia Forest.They are so identical in looks that they are easily mistaken one for the other by people who don't know either of them well, but so different in mannerisms, behaviour, and speech, that anyone who does know them well can tell them apart immediately. Lawrie, a skilled actor, can more easily pass for Nicola when she wants to than Nicola can for Lawrie - a plot point in one of the books.
* In the original Story ''Literature/LottieAndLisa'' by Erich Kästner (aka "Das doppelte Lottchen"), the titular twins had extremely different personalities, which made their [[spoiler:place change]] all the more hilarious, and later difficult. Lisa was a cheerful, bratty tomboy while Lottie was a sensible, serious, prim and proper girl who behaved almost like a grown-up. Most film versions omit this aspect.
* Eve and Alexandra in ''Master of the Game'' are a CainAndAbel crossover version -- Alexandra is sweet, honest, and innocent, with no ambitions towards running Kruger-Brent, Ltd. Eve is consumed by ambition and the desire to be the center of everyone's attention, and starting from the age of ''five'' she's determined to kill and/or ruin Alexandra, who's in the way. (Incidentally, author Creator/SidneySheldon co-created the much gentler TropeNamer years before.)
* Abby and her twin sister Anna of ''Literature/TheBabySittersClub''. Anna is musical, bookish, and introspective; Abby is athletic, noisy, and enjoys babysitting. About the only things they have in common are that they both have scoliosis and poor eyesight.
* Dawn and Eve of Creator/PiersAnthony's ''Literature/{{Xanth}}'' are literally as different as day and night, from the fact that Dawn is red haired and green eyed, and is like the morning, while Eve is black haired and eyed and is like the night.
* Belle and Samuel from ''Literature/{{Rolitania}}'' are very different, indeed. Belle is popular, sociable, and has thousands of Instagram followers. Samuel, on the other hand, is the ButtMonkey of the class, a former NEET, and can hardly speak for himself when confronted.
* Both the books and TV version of ''Literature/SweetValleyHigh'', with the Wakefield twins Jessica and Elizabeth.
* The ''Literature/TortallUniverse'' gives us:
** In ''Literature/SongOfTheLioness'', Alanna the down-to-earth ActionGirl who dislikes magic, academic pursuits and social games, and Thom the fame-hungry career mage and scholar who thinks he's AboveGoodAndEvil.
** A deliberate subversion of this trope in the next generation with Alanna's [[UntoUsASonAndDaughterAreBorn daughter and son]] Alianne and Alan in ''Literature/DaughterOfTheLioness'', who are neither this nor SingleMindedTwins. WordOfGod says this was to point out that there's not always some mystical weirdness about twins.
* In Pierce's Literature/CircleOfMagic universe, Nia the shy, retiring one (with woodworking magic) and Jory the hyperactive, overeager one (with cooking magic).
* Literature/{{Tatterhood}} and her [[NoNameGiven unnamed twin sister]] from the Norwegian fairytale are a perfect example of this: Tatterhood is an ugly, but spirited princess who rides on a goat and singlehandedly beats up hordes of trolls and witches, while her sister is a beautiful, but helpless DamselInDistress. Despite this, the twins are inseparable and get along famously.
* Amoret and Belphoebe in ''Literature/TheFaerieQueene'': The former was adopted by Venus, the Goddess of Love and Beauty, and became a ProperLady. The latter was adopted by Diana, the Goddess of Maidenhood and the Hunt, and became an ActionGirl.
* Robert L. Stevenson's ''Literature/TheStrangeCaseOfDrJekyllAndMrHyde'': Dr. Jekyll and Mr. Hyde may be considered a variation of this trope.
* Emmeline and Adeline March from ''Literature/TheThirteenthTale'', who are totally passive and excessively violent respectively.
* The twins in ''Literature/FlowersInTheAttic'' Cory is quiet, polite and gentle. Carrie is loud, adamant and forceful.
* WebSerialNovel ''Literature/CorrespondenceFromTheGoddess'' has Lydia Devin, the main character, a snarky aimless twentysomething who slowly gains divinity-level powers over the course of the story, and Elana Devin, her twin sister, who is perfect as perfect can be but doesn't seem to have any powers at all.
* Billie and Joe from ''Literature/TheCallOfTheWild'' are said to be as different as day and night. Billie is a sweet but timid dog, but his brother Joe is spiteful and aggressive, even keeping evil Spitz at bay.
* Creator/JacquelineWilson 's ''Double Act'' has [[TomboyandGirlyGirl Tomboy Ruby and Girly Girl Garnet]]. This becomes more apparent when [[spoiler:Ruby cuts off her braids to distinguish herself further from her sister]].
* Squirrelflight and Leafpool from ''Literature/WarriorCats'', especially when they're apprentices. Squirrelflight is sharp-tongued and energetic, and becomes a warrior, while Leafpool is calm and more reasonable, and becomes a medicine cat. They're even compared directly to fire and water once.
* [[Literature/TheMillenniumTrilogy The Millenium Quadrology]] has Lisbeth and Camilla Salander. Lisbeth is a brilliant hacker with NoSocialSkills who stands up for abused women and children, and has a strong moral code. Camilla is highly manipulative, does not care about those she considers "weak", and has very little in terms of morals [[spoiler:being the leader of a Russian crime syndicate]].
* Cilla and Tina from ''Literature/IMissYouIMissYou''. Cilla is down to earth and is interested in directing theater while Tina is mainly interested in boys and can be quite petulant.
* Creator/JackieCollins ' novel ''Chances'' has Lucky and her brother Dario. Lucky has dark hair and a vigorous personality, and she's extremely ambitious. Dario is blonde and a slacker, more interested in his Porsche than the family business.
* Tobi and Toklo from ''Literature/SeekerBears''. Tobi is a timid, quiet IllBoy while Toklo is adventurous and more than a little grumpy.

[[folder:Live Action TV]]
* Madison and Vida Rocca from ''Series/PowerRangersMysticForce''. Madison is calm and thoughtful, V is on a permanent sugar high and goes rushing into things with a mad grin.
* Theo and Luen Martin from ''Series/PowerRangersJungleFury''. Theo is calm, focused and studious, while Luen is adventurous and outgoing.
* ''Series/SisterSister'', with Tia and Tamera Mowry. SeparatedAtBirth, Tia being the mature, smart and responsible one while Tamera was irresponsible and BookDumb. Their characters in the Disney film version of ''Film/TWitches'' are basically the same.
* ''Series/TwoOfAKind'' and ''Series/SoLittleTime'', both with Mary-Kate and Ashley Olsen. The twins played this up in most of their TV-movies as well. Ashley nearly always played the responsible one with Mary-Kate as the wild one. This finally got reversed in ''Winning London'' where Mary-Kate was bookworm and straight-A-student Chloe while Ashley was relaxed {{Ladette}} Riley.
* A few Creator/DisneyChannel shows:
** ''Series/TheSuiteLifeOfZackAndCody'': Sprouse twins. Dylan as Zack the jock and Cole as nerdy Cody.
*** Only the Tipton Hotel's Suite Life made them stereotypes (as ''On Deck'' [[{{Flanderization}} made them more similar and realistic]]).
*** It has been said that each Sprouse twin is a better fit to the ''opposite'' Martin twin.
*** {{Lampshaded}} in "Grounded on the 23rd Floor":
-->'''Zack:''' I swear, we're not related.
** Also used in ''Series/LivAndMaddie'' and ''Series/IDidntDoIt''. Creator/PattyDuke herself would appear on ''Liv and Maddie'' [[ActorAllusion as the twins' grandmother and her own twin sister]].
* ''Series/{{Friends}}'' gives us twin sisters Phoebe and [[Series/MadAboutYou Ursula]], the first being a (mostly) light-hearted and cheery {{Cloudcuckoolander}}, with the second being mean-spirited, thoughtless, and, in Phoebe's words, a "real high-powered, driven career-type" (she's a waitress).
* The '80s sitcom ''Series/DoubleTrouble''. Identical twins Jean and Liz Sagal (younger sisters of Katey Sagal of ''Series/MarriedWithChildren'') play identical twins Kate and Allison. Allison is serious and bookish; Kate is happy-go-lucky.
* [[spoiler:BigGood Jacob and BigBad The Man in Black, Jacob's fraternal twin]] from ''Series/{{Lost}}''.
* ''Scoundrels'', Cal and Logan West, an incompetent burglar/criminal, and an intelligent defense lawyer, respectively. The characters are so different, you can barely tell they're played by the same actor.
** This is a remake of a New Zealand show, ''Outrageous Fortune'', with Van and Jethro West in the same roles (also played by the same actor).
* The good and evil brothers in ''Series/TheWanderer''. Dispite both being played by Bryan Brown it isn't at 'all' difficult to tell Adam from Zachary, the evil cackle gives it away every time.
* ''Series/{{iCarly}}'': Sam and Melanie Puckett. The former is almost permanently grouchy, borderline sociopathic, and sarcastic, while the latter is upbeat, outgoing, and perky. Notably, Carly says she can't believe that they're sisters. They agree.
* Lee and Leah, the SeparatedAtBirth twins from the British teen show The Gemini Factor, a rebellious boy and a teacher's pet girl, with a sprinkling of TwinTelepathy. They also tended to wear black and white, respectively.
* Katie and Emily Fitch from ''Series/{{Skins}}''. In the beginning Katie is portrayed as the shallow, popular, boy-obsessed AlphaBitch, and Emily as the sweet, socially-awkward ShrinkingViolet (and closet lesbian) who does everything her bossy sister says, until she comes out of the closet and begins dating her girlfriend Naomi. But when both girls suffer a series of [[BreakTheCutie severe personal setbacks]] they handle it very differently, with Katie gaining a lot more depth and maturity, having to hold the family together virtually on her own, and Emily becoming more and more angry and destructive.
* Cathy and Patty Lane from ''Series/ThePattyDukeShow''. "But they're cousins, Identical cousins all the way. One pair of matching bookends, Different as night and day."
** ''Series/{{SCTV}}'' parodied the show, running promos for "Identical Bellhops", "Identical Cheese Hostesses" and "Identical OPEC Oil Ministers", all shoehorning their titles into the familiar theme song.
* Sutton and Emma on ''Series/TheLyingGame''. Sutton is a BookDumb RichBitch IceQueen AlphaBitch while Emma is sweet, caring and intelligent. The only things they have in common are a love for tennis and [[SiblingTriangle for Ethan]].
* ''Series/TheBigBangTheory'' has Sheldon and Missy Cooper. Missy is an athletically built, charismatic, SouthernBelle whom their mother once described as being "dumb as soup." Sheldon is the page image for InsufferableGenius, a HollywoodNerd, with NoSocialSkills to the point he does not understand typical human interaction.
* Patricia and Piper Williamson from ''Series/HouseOfAnubis''. Patricia is the bold, [[TomBoy Tomboyish]], defiant and rude one; Piper is highly talented in piano, very sweet and polite, and much less inclined to make big moves out of fear of failure than her [[DeterminAtor Determinator]] sister is. It's pretty obvious when you compare their crushes- Patricia likes JerkWithAHeartOfGold Eddie, while Piper likes the NiceGuy ClassClown Alfie.
* Max and Phoebe of ''Series/TheThundermans'' share only one trait: their superpowers.
* ''Series/{{Bewitched}}'' had identical cousins Samantha and Serena. Sam was (mostly) happy maintaining [[Main/{{Masquerade}} the Masquerade]] as a suburban housewife, while Serena was a wild party-girl who regularly abused her magic.
* Jeannie and her sister on ''Series/IDreamOfJeannie''. Jeannie is a blonde who wears pink, and while she can be selfish and impulsive, is generally sweet and wants the best for Major Nelson. Her sister is a brunette who wears green and is a lot more malicious, self-centered, and sexually aggressive (constantly going after Tony, who's not interested), and causes a lot of problems whenever she shows up.
* Turns out that every Spellman family member is born with a Polar Opposite Twin in ''Series/SabrinaTheTeenageWitch''. The most glaring example is Zelda and Jessebelta. Zelda is an intellectual who values knowledge and culture. Her twin on the other hand speaks like a SouthernBelle and describes herself as a "bad, bad girl".

* In The Venetian twins from Carlo Goldoni the new spin on the theme of the accidental [[TwinSwitch twin switch]] is that they are completely different in their way of acting and talking and one is considered really well mannered and intelligent while the other is seen as tactless and [[TooDumbtolive stupid]]

[[folder:Newspaper Comics]]
* In the ComicStrip/{{Scamp}} ([[SpinOffspring Son of]] Disney/LadyAndTheTramp) comics (Specifically the newspaper continuity), Scamp and his siblings are two sets of these. Twin Sisters Fluffy and Ruffy are dignified and tomboyish, respectively, while non-identical brothers Scamp and Scooter are adventurous and timid, respectively.

[[folder:Religion and Mythology]]
* OlderThanFeudalism: Myth/ClassicalMythology - Artemis, a moon goddess and twin sister of Apollo, a sun god, though originally the Greeks did not identify them with celestial bodies.
* Myth/HebrewMythology has Shachar and Shalim, the twin gods of dawn and dusk.
* In Literature/TheBible, we have Jacob and Esau. Esau is the older one, and he displays a CarpetOfVirility and is [[ARealManIsAKiller a skilled hunter]]. Jacob is younger, and isn't so hairy and prefers to use his [[GuileHero brains over brawn]]. [[spoiler:Which enables him to receive Esau's birthright.]] They were at odds with one another ''before they were even born''--God explains to their mother that her difficult pregnancy came from them fighting in the womb. [[spoiler:Though they manage to make peace... several years later.]]
** According to Jewish tradition, Jacob's [[KissingCousins cousins/wives]], Rachel and Leah, were also twins and had similarly disparate personalities, the former being a quiet introvert, the latter more gregarious and emotional.
* Nissien and Efnissien from the Mabinogion. Their names literally mean "peace" and "not-peace" which, funnily enough, highlights the main theme of the story. Not much is said of Nissien. His sociopath brother, on the other hand...

[[folder:Tabletop Games]]
* The elves of ''TabletopGame/{{Warhammer}}'' fantasy really seem to be prone to this trope.
** High Elf Heroes Tyrion and Teclis are identical twins with radically opposing personalities and skills. Tyrion is proud, fair, noble, athletic and well-spoken, while Teclis is dark, feeble, scholarly, caustic-tongued and bitter. The one is the greatest warrior and commander of his race but has no talent for sorcery, the other is a poor fighter but [[TheArchmage the most powerful living wizard in this age of the world]].
** Another example can be found in the 5th edition Tears of Isha campaign pack, which focuses on the war between Calaidan and Kaldor, twin scions of the High Elf house Coraith, one of whom defects to Naggaroth and becomes an evil Dark Elf while the other inherits the family estates and continues their proud High Elven traditions.
** The Wood Elves have Naestra and Arahan, polar opposite personalities in identical bodies produced when Naestrahan was split into two women due to an unknown event deep in the woods of Athel Loren. They ride a dragon.
* ''TabletopGame/{{Ravenloft}}'s'' Laurie and Gennifer Weathermay-Foxgrove are identical twin monster-hunters and proteges of Rudolph van Richten. Laurie is an exuberant tomboy, fencer and pistoleer who dresses like a man, while Gennifer is a demure, sober-minded wizard with closets full of conservative dresses. [[spoiler:Also, Gennifer may be an unwitting lycanthrope.]]
* A number of the gods in pre-Fourth Edition ''TabletopGame/ForgottenRealms'' were opposed siblings, though they usually looked noticeably different--Shar and Selune (the moon and the dark), Tymora and Beshaba (good and bad luck), Vhaeraun and Eilistraee (drow thieves, often male, and redeemed drow, often female).

* Not ''technically'' twins, but Toys/MonsterHigh has Jackson Jekyll and Holt Hyde, complete opposites who share a body. While Jackson is quiet and nerdy, Holt is outgoing and upbeat. They even used to have a "night and day" system of who was the one in control, although that has since changed.

[[folder:Video Games]]
* The ''VideoGame/DevilMayCry'' series [[RedOniBlueOni Hothead Dante and calculating Vergil]].
* ''VideoGame/FinalFantasyIV'': Palom and Porom. Palom is the bouncy, mischievous user of black magic, and Porom is the serious-minded user of white magic.
** ''VideoGame/FinalFantasyDimensions'' contained gender-swapped [[{{Expy}} Expies]] of these characters in soft-spoken, serious white mage (at game start) Dusk and his snarky, energetic black-magic-using (again, at game start) twin sister Alba.
* The ''Manga/GetBackers'' game ''Jagan Fuuin'' The Mikado twins: Daisuke is very compassionate and sweet and easily befriends the main cast, whereas his identical twin Kyosuke is cold, emotionless, rarely speaks, and seems robotic. It's eventually revealed [[spoiler:that the twins are the same person, namely Kyosuke, who, due to fear of their immense spiritual power, were split into two entities, the "body" and "soul". Daisuke was the soul, and once they were merged back into Kyosuke, he acted much like his "brother" did]].
* ''VideoGame/MOTHER3'': Lucas is [[ShrinkingViolet shy and withdrawn]], while Claus is [[{{Hot Blooded}} rash and reckless.]]
* ''Franchise/StreetFighter'': Hyperactive Yun and brooding Yang of the Lee Brothers.
* ''VideoGame/VampireTheMasqueradeBloodlines'': The Voerman sisters, stoic businesswoman Therese and emotional party-girl Jeanette, from You eventually discover [[spoiler:Therese is a Malkavian, a member of a clan of vampires known for insanity, and her "sister" Jeanette is actually her SplitPersonality. With [[TalkingTheMonsterToDeath high enough Persuasion skill]], you can convince the "sisters" to cooperate instead of one "killing" the other.]]
* The ''VideoGame/WarCraft'' universe. Malfurion and Illidan Stormrage, the night elf twin brothers. The former leads his people to druidism as an alternative to the dangerous magic that brought the [[LegionsOfHell Burning Legion]] to Azeroth. The latter insists on tapping both that magic and the power of the demons themselves, is imprisoned for centuries and eventually becomes an EvilOverlord.
* ''VideoGame/TheSims2: University'':
** The Davis twins are described as "different as night and day" within the game, yet they actually have identical personalities and aspirations. The only real difference between them is their appearances and what they like to talk about. Similarly, the Pleasant twins in Pleasantview are supposedly very different, but have very similar personalities.
** In Strangetown there's Lola and Chloe, in the Singles household, although [[ContinuitySnarl whether or not they are actually twins is somewhat debatable]]. Lola is MarriedToTheJob, while Chloe is a HardDrinkingPartyGirl.
* ''VideoGame/FireEmblem'':
** Adean and Briggid from ''VideoGame/FireEmblemJugdral''. Adean is a sweet WhiteMagicianGirl who didn't train as an ActionGirl because it simply didn't fit her kind nature, while Briggid was a stubborn {{Ladette}} that was first kidnapped and then raised among pirates.
** ''VideoGame/FireEmblemElibe'' introduces another pair in Lugh and Ray. Lugh is a [[CheerfulChild cheerful]] mage (specializes in elemental magic), while Ray is a cold and calculating shaman (specializes in dark magic).
** ''VideoGame/FireEmblemFates'' features ''two'' sets, though they're actually non-identical in both cases:
*** Nohr natives [[NinjaMaid Felicia and Flora]] are more adept at combat and housework, respectively.
*** From Hoshido, Kaze is fairly calm and collected, while Saizo is more HotBlooded and blunt. Fittingly, [[RedOniBlueOni Saizo's hair is red and Kaze's is green]].
* The Bruiser Brothers in ''Super VideoGame/PunchOut''. Rick Bruiser is outgoing and hot-blooded, while Nick Bruiser is quiet and cold.
* The ''Franchise/{{Neptunia}}'' series has the extrovert Ram and the shy Rom. These twins are also a huge source of [[AnnoyingYoungerSibling irritation]] for their big sister, Blanc.
* Greg and Sidney Kasal from ''VideoGame/TraumaCenter''. Sidney is serious and straight-laced, while Greg is far more relaxed.
* The Ryō twins from ''VideoGame/SenranKagura''. Ryōbi is a {{sadist}} who hates [[ACupAngst her flat chest]], where as Ryōna is a masochist who loves [[BigBreastPride her large rack]].
* Saku and Bo from ''[[VideoGame/DotHackGU .hack//G.U.]]'' are twins taking turns playing one character, Sakubo. You can tell whose is logged on by the character's eyes (Saku has TsurimeEyes, Bo has TaremeEyes), hat (Saku has the "sun" side pointing up, Bo has the "moon" side), and [[RedOniBlueOni their general attitude towards you]].
* ''VideoGame/TheElderScrollsVSkyrim'' has fraternal twin brothers Vilkas and Farkas, in the Companions guild, who are a combination of this trope and RedOniBlueOni. Vilkas is very intelligent, but abrasive, hot-blooded, and not especially friendly to new arrivals. Farkas is generally regarded as being dumb muscle, but much more laid back, difficult to anger, and sweet-natured.
* The titular ''Franchise/SuperMarioBros''. Mario is brave, outgoing and hot-blooded, Luigi is cowardly, timid and shy.
* The Yorigami sisters from ''VideoGame/{{Touhou}}''; Jo'on is prideful and domineering while Shion is gloomy and pessimistic.
* The titular VideoGame/{{Cuphead}} is known for being the hot-blooded, courageous, and sometimes devious older twin brother. [[http://kingfeatures.com/licensing/licensed-brands/cuphead/ "A shoot-first-and-ask-questions-later type of guy."]] Mugman is known for being the good-humored, industrious, and rascally younger twin brother. "Calculated in battle and a tricky adversary." While both of them have a rebellious streak, Mugman does try to stop Cuphead when the latter gets too overconfident when betting against the Devil.

[[folder:Visual Novels]]
* Dahlia Hawthorne and Iris from ''VisualNovel/PhoenixWrightAceAttorneyTrialsAndTribulations''. Dahlia is [[spoiler:a ruthless sociopath who has been using her cute and innocent image to get away with murder, literally, for years]] and Iris is [[spoiler:sweet, gentle, and compassionate, and honestly fell for the lead character while posing as her sister because she needed him for a master plan but didn't want to be bothered with actually seducing him]].
* ''VisualNovel/{{Clannad}}'': Kyou and Ryou. Long-haired {{tsundere}} and short-haired easily-embarrassed sweetheart.
* ''VisualNovel/HigurashiWhenTheyCry'': Mion and Shion Sonozaki. Mion is [[{{Tomboy}} assertive]] and [[LargeHam bombastic]], the leader of her TrueCompanions; Shion is sly and [[TheTease seductive]], preferring to [[{{Troll}} manipulate people subtly]] rather than openly command them. It's even more odd, when you realize that [[spoiler:they're acting like each other, thanks to a TwinSwitch they made when they were younger.]]
* ''VisualNovel/{{Tsukihime}}'': Kohaku and Hisui. One is carefree, energetic and always smiling, while the other is silent with little expression. It's also odd, when you consider that [[spoiler:they switched places from when they were children: Hisui was originally carefree and cheerful, while Kohaku was shy and untalkative. Then they got older and things got worse for them (specially [[RapeAsBackstory Kohaku]]), so Hisui buried her emotions to help Kohaku stay stable while Kohaku hid her very darkened TrueSelf behind a facade of happiness.]]
** Averted in ''Flower of Thanatos'' sidestory, where Kohaku and Hisui synchronize to point where they are no longer themselves and their personalities fuses into one. Becoming SingleMindedTwins instead.
* Ember and Daystar in the ''ComicBook/ElfQuest'' visual novels. Ember is active, bold and like her name in looks and attitude. Brother Daystar is mystical and calm, and blond with pale skin.
* ''VisualNovel/LittleBusters'' has [[spoiler:Haruka and Kanata]], who form an AllWorkVsAllPlay duo. In reality, though, these roles were mainly either in response to or outright enforced by [[spoiler:their abusive extended family and in the end when they are able to move on from their past and accept one another as friends their personalities become a lot less extreme. Not entirely, though.]]
* ''VisualNovel/EfAFairyTaleOfTheTwo'': The Shindou sisters. Chihiro is an [[TheIngenue ingenue]], Kei is a {{Shorttank}}.
* The Kazato twins from ''VisualNovel/IfMyHeartHadWings''. Asa is the timid one, and Yoru is the blunt one. [[ThemeTwinNaming Their differences in personality are literally night and day.]]
* Pictured above: ''VisualNovel/TokimekiMemorial 2'' has the twin sisters Miho and Maho Shirayuki. Miho is a sweet, daydreaming girl who believes in fairies, loves [[FortuneTeller fortune-telling]] and plushies, and dreams of becoming a scenario writer of stories for children. Maho is a playful, blunt and mischevious street-wise girl, who enjoys [[TheFashionista fashion]] and window-shopping, and dreams of becoming a model.


* ''Webcomic/ItsWalky'': Fraternal twins Beef and Walky. Justified as Beef was actually switched at birth with Walky's real twin Sal as part of Mrs. Walkerton's SeparatedAtBirth gambit, Sal and Walky later, after the truth was uncovered, and used to set up some NotSoDifferent moments between the two of them.
** Sal and Walky again in ''Webcomic/DumbingOfAge'' despite not being raised apart this time around.
* ''Webcomic/LnM'': L is energetic and childish, M is laid back and precocious.
* Molly and her clone/twin Galatea in ''Webcomic/TheInexplicableAdventuresOfBob'' were raised separately. Both are brilliant, naive, and devoted to Bob, Jean, and each other. But while Molly is upbeat, friendly, and gentle, Galatea is paranoid and megalomaniacal.
* ''Webcomic/TheOrderOfTheStick'' has Elan and Nale, who look the same except for [[BeardOfEvil a goatee]] on Nale, but their personalities are complete opposite. Elan is good, naive and GenreSavvy, while Nale is an evil and scheming. [[http://www.giantitp.com/comics/oots0725.html It turns out]] that Elan got his goodness from his mother and his genre savvyness from his father, while Nale got his evilness from his father and his affinity for schemes from his mother. Their opposite is also indicated by [[SdrawkcabName their names]].
* Gogo and Didi from ''Webcomic/{{Bomango}}''. Gogo is a BoisterousBruiser with a [[HairTriggerTemper short fuse]], a heterosexual {{Ladette}}, and a JerkWithAHeartOfGold who acts as TheId in their FreudianTrio (Andy is TheEgo). Didi is a polite perky scientist and a LipstickLesbian who acts as TheSuperEgo. They're a weird example though, in that Didi slowly budded off Gogo which would make her some kind of clone/daughter, but they see each other as twins and have a more sisterly relationship.
* The Hong twins in ''Webcomic/SharpZero''. Elliot is dorky, unorganized, and believes others see him as [[TheUnfavorite the evile twin]] and a screw up. Marcus is the put together Mom Friend, was a straight A student, and never gets in trouble.
* ''Webcomic/WelcomeToRoom305'' has the HotBlooded [[TheLadette ladette]] Yoona as the older twin to Yoon Sung, a ([[NotSoStoic normally]]) [[TheStoic stoic]] young man. As young children Yoona was a tomboy while Yoon Sung was a sensitive crybaby. Both twins have tempers when they want to though.
* Sikue and Tatsuma in ''Webcomic/BeyondBloom'', are not identical, but were "born" together. They are polar opposites in many ways, one being loud and aggressive, while the other is quiet and gentle. This is especially emphasized in their magic. Tatsuma has offensive abilities while Sikue's are defensive.

* In Machinima/RedVsBlue, North is a relatively NiceGuy who is more than willing to put himself in harm's way to save others, while South tends toward ChronicBackstabbingDisorder, [[spoiler:with tragic results for her brother]].
* [[http://akaichounokoe.deviantart.com/art/Doki-in-Victorian-style-elegance-272962375?q=boost%3Apopular%20((doki)%20AND%20(by%3Aakaichounokoe))&qo=17 Doki]] is much different than her twin sister [[http://akaichounokoe.deviantart.com/gallery/?q=lolita#/d4ivzfz Toki.]] Both dress somewhat alike but wear different colors: black or purple for Toki, reflecting her enigmatic, yet domineering but secretly kindhearted personality, and pink and white for Doki, reflecting her kind, gentle, and angelic personality. It isn't just that, according to Amoridere, Doki is more morally grounded than Toki is (as in, she wouldn't stoop to schemes or anything illegal to meet her demands, whereas the latter probably and more often than not would), along with being more levelheaded, and, Toki, well, she is described as being one who does what she wants, whenever she so wants to do it, consequences be damned. It was also noted that, when the two were children, Doki was always the shy one, while Toki was more assertive.
* [[http://akaichounokoe.deviantart.com/art/Punk-Brownie-461301053?q=sort%3Atime%20((brownie)%20AND%20(by%3AAkaiChounokoe))&qo=0 Brownie]] is very much different than her sister, [[http://akaichounokoe.deviantart.com/art/Everyone-is-Beautiful-regardless-279145781?q=sort%3Atime%20((brownie%20and%20brittani)%20AND%20(by%3AAkaiChounokoe))&qo=2 Brittani,]] in that Brittani is described as being prim and proper, while Brownie is described as tomboyish. Apparently, them looking different than one another is supposed to reflect that.
* [[http://killerbunnies.wikidot.com/wiki:kamu-and-kanran Kamu and her twin sister Kanran]] are somewhat similar in some personality traits, however, Kamu can be said to be more levelheaded and is typically polite whenever needed, whereas Kanran is typically gluttonous, rude, and usually acts before thinking, making her rather foolish. Likewise, [[http://akaichounokoe.deviantart.com/art/Killerbunny-LVII-Miren-and-Citrine-Thomas-508368268 Miren and Citrine,]] with the former being described as being "LeanAndMean" and the latter being "bright and bubbly".
* Vex and Vax from ''WebVideo/CriticalRole'' play RedOniBlueOni to each other. [[Creator/LauraBailey Vex]] is [[GenkiGirl excitable]], [[TheCharmer flirtatious]], and [[HotBlooded emotional]]. While [[Creator/LiamOBrien Vax]] can be all these things in the right contexts, he's more prone to being [[TheStoic composed]], [[DeadpanSnarker sarcastic]], and [[PragmaticHero pragmatic]]. The twins love each other dearly, though.
** [[spoiler:They also become champions of gods that are practically night and day -- Vax under the god of death, the Raven Queen, and Vex under the god of the sun, Pelor.]]

[[folder:Western Animation]]
* ''WesternAnimation/AmericanDragonJakeLong'': Oracle twins Kara and Sara. The gothic Kara foresees good events in the future and is always gloomy because everything good about to happen gets spoiled for her while blonde and preppy Sara forsees the bad events and is always so happy because any time the horrible things she sees ''aren't'' happening is absolutely fantastic in comparison.
* ''WesternAnimation/TheAngryBeavers'': Dagget and Norbert, twin beavers, the latter of whom is sensible and collected and the former of whom is essentially insane.
* In ''WesternAnimation/BoJackHorseman'''s ShowWithinAShow, ''Mr. Peanutbutter's House'', the titular character adopts two human twins Zoe and Zelda, the first of whom is outgoing and cheerful, while the latter is sullen and brooding. It became an InUniverse [[MemeticMutation meme]] in the show's fanbase to classify people as either "Zoes" or "Zeldas" depending on their outlook and personality.
%%* ''WesternAnimation/TheCrampTwins'': The entire premise.
* ''WesternAnimaton/CatDog'': Cat and Dog, [[ConjoinedTwins conjoined twins]] who are essentially the opposite of each other. Cat is sophisticated, risk-avoidant, and short-tempered. Dog is uncultured, reckless, and chipper.
* ''Day and Night'': The [[WesternAnimation/PixarShorts Pixar animated short]] actually took this literally, featuring an encounter with two traditionally-animated figures, through which views of a CGI world during different times of the day can be seen.
* ''WesternAnimation/DragonTales'': Zak and Wheezie, who are even very different colors despite being conjoined. Zak is green and uptight, while Wheezie is purple and a GenkiGirl.
* ''WesternAnimation/ElfQuest'': Suntop and Ember actually brought the {{Media Watchdog}}s down on them when their creators tried to get an AnimatedAdaptation going. Turns out Saturday Morning Cartoons is not the time to reverse genders roles and have a spunky little girl and a mild, intuitive little boy. Or, for that matter, twins with different skin colors.
* ''WesternAnimation/TheFairlyOddParents'': Timmy's more serious and responsible fairy godparent Wanda has a twin sister named Blonda who's a fun-loving movie star.
* ''WesternAnimation/FreakyStories'' features a story about a pair of twins who take advantage of their wildly different interests in talents by using TwinSwitch to appear to be one perfect person.
* ''WesternAnimation/KimPossible'': Gemini identifies himself as being opposite his twin sister Dr. Director in every possible way, which is mostly accurate. They don't even look much like siblings, let alone twins.
* ''WesternAnimation/{{Kaeloo}}'': Rabbit sisters Pretty and Eugly. Eugly is a kind, friendly GonkyFemme and Pretty is a beautiful yet selfish and manipulative AlphaBitch who is hated by the rest of the cast.
* ''WesternAnimation/MyLittlePonyFriendshipIsMagic'':
** Pinkie Pie and her fraternal twin sister, Marble Pie. Pinkie Pie is bright pink, exuberant, and outgoing, while Marble Pie is a dull gray, reserved, and very shy.
** According to the [[ComicBook/MyLittlePonyFriendshipIsMagicIDW IDW Comics]], the mild-mannered schoolteacher Cheerilee has an identical twin sister named Cherry Blossom who's a professional wrestler.
%%* ''WesternAnimation/MuppetBabies'': Scooter and Skeeter. They sang a song dedicated to this in the episode about opera.
* ''WesternAnimation/TheVentureBrothers'': Thanks to DivergentCharacterEvolution - Dean has slowly become a nervous, cowardly NonActionGuy while Hank has evolved into a SmallNameBigEgo who [[TooDumbToLive leaps into danger]] at the slightest provocation.
** Also bitter, unsuccessful, frail Rusty and the much more skilled and charismatic JJ. It is implied that JJ got all the best DNA from their father, despite having spent decades as Rusty's [[BodyHorror conjoined foetal twin]].
* ''WesternAnimation/GravityFalls'':
** The series has [[AdorablyPrecociousChild smart, serious Dipper]] and [[GenkiGirl ditzy, energetic Mabel]].
** It's later revealed that [[spoiler:Grunkle Stan was also a twin. [[GenerationXerox His brother, the real Stanford Pines, was a genius, while Stanley was definitely not.]] [[TearJerker Their different interests were what eventually separated them, and Mabel ends up worried her relationship with Dipper might crumble like theirs]].]]
* ''WesternAnimation/YinYangYo'' has [[BadassBookworm smart]] NeatFreak Yin and her [[BookDumb less intelligent]] [[BloodKnight violence loving]] brother, Yang.
* ''WesternAnimation/TheLoudHouse'' has identical twin sisters, [[{{Tomboy}} Lana]] and [[GirlyGirl Lola]] --
** Lana's probably the most masculine and tomboyish of her sisters after Lynn, is much nicer and more outgoing, and loves getting dirty.
** Lola, who's arguably the most feminine of the ten sisters, on the other hand, [[PrincessPhase dresses like a princess]], regularly competes in beauty pageants and is far more conceited than her twin is.

[[folder:Real Life]]
* ''Series/AmericasNextTopModel'': Amanda and Michelle Babin, who were contestants on Cycle 7. Michelle is tomboyish and more outgoing, whereas Amanda is more feminine and introverted. Michelle also came out of the closet during the course of the show, just to add another night and day difference to the mix.
* Nikki and Brie, the Wrestling/BellaTwins of ''Series/TotalDivas'' and WWE fame. Brie is a GranolaGirl, who loves being a hippie and CountryMouse - but also is a HardDrinkingPartyGirl. Nikki is TheFashionista and power lifter, who loves anything expensive - while preferring wine and desserts to excessive drinking. Their workout methods are different, with Brie opting for yoga and cardio while Nikki enjoys power lifting to maintain AmazonianBeauty.
* ''Music/TokioHotel'': Bill and Tom Kaulitz are identical twins, but dress and wear their hair differently, just to name one difference, to the point that some people don't believe they're identical. One of the more notable differences is Bill's focus on "true love" while (until recently) Tom was only looking for love of the one-night variety.
* The public perception of [[LondonGangster the Kray twins]] has traditionally had Ronnie, the younger twin, as pure AxCrazy psychopath, and Reggie as quieter and more AffablyEvil, even acting as something of a MoralityChain to his brother. There's not necessarily much evidence of this, but that just makes the fact that people ''want'' it to be the case an example of this trope in action.
* Twin musicians Chuck (bass) and John (drums) Panozzo of Music/{{Styx}}. Chuck is soft-spoken and more down to earth, while John was a wild man and more outgoing.
* Happens to twins in RealLife, as twins growing up together try to develop different personalities to distinguish themselves from their sibling. However this is usually subverted in identical twins - twins that came from the same zygote, not from two different zygotes in the womb - as they are practically genetically identical copies of each other and will share many similarities in personality and preferences even if they act differently. National Geographic has done an article on it.