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[[quoteright:350:[[ComicBook/UltimateSpiderMan2000 https://static.tvtropes.org/pmwiki/pub/images/Opposite_Sex_Spiders_5463.png]]]]

->''"I'M TOO YOUNG AND TOO MALE TO BE THE MOTHER OF A SEVENTEEN YEAR-OLD FEMALE ME!"''
-->-- '''Elliot''', ''Webcomic/ElGoonishShive''

While clones and most other sorts of sci-fi duplicates are identical to the original character and, by definition, the same sex, exceptions do exist. The idea dates at least from ''Nine Lives'' by Creator/UrsulaKLeGuin (1969), where recognized geniuses are routinely cloned in large mixed-sex groups and ''Literature/TimeEnoughForLove'' by Robert Heinlein (1973), where the [[ScrewYourself opposite sex clones become sexual partners]] of the original. Nowadays the trope is more often used as a quick way of producing a DistaffCounterpart rather than for titillation. Merely being of the opposite sex of his or her original greatly increases the chance of the clone surviving the denouement and showing up in a future story. This way writers can invoke the FirstLawOfGenderBending without sacrificing an original-sex character they've grown fond of.

Biologically, this is somewhat plausible for male-to-female cloning, because a male has an X-chromosome that can be doubled to produce a female clone, as in Garrett's poem and Heinlein's novel.[[note]]Strictly speaking, you don't even need the second X chromosome, though omitting it is a good way to kill 99% of your fetuses and give the surviving 1% [[https://en.wikipedia.org/wiki/Turner_syndrome Turner syndrome.]][[/note]] Alternatively, the SRY gene, which causes maleness, can be removed from the Y chromosome. For female-to-male cloning, the Y chromosome would have to come from another subject — most ideally the donor's father, as she would have inherited her father's Y chromosome had she been born male — but this would technically [[MixAndMatchMan not be a pure clone]]. A Y chromosome could be formed from one of the X-chromosomes, which would require absurd amounts of genetic engineering and amount to simple fiat anyway. Alternatively, the [[https://en.wikipedia.org/wiki/XX_male_syndrome SRY gene can be implanted in one of the X chromosomes]] to create a sterile clone that appears male. Somewhat fittingly when given the above, the majority of Opposite-Sex Clones in fiction are female.

If the Opposite-Sex Clone retains the memories and personality of the original this is effectively a GenderBender, at least from the perspective of the clone.

See CloneAngst for when said clone is upset about their nature. Subtrope of ModifiedClone and OurClonesAreDifferent. Compare DistaffCounterpart, HalfIdenticalTwins and ScrewYourself. See also TransAudienceInterpretation, as that is a common fan explanation as to why this trope occurs.

----
!!Examples:

[[foldercontrol]]

[[folder:Anime & Manga]]
* In ''Anime/MagicalGirlLyricalNanohaStrikers'', [[EvilGenius Uno]] of the [[QuirkyMinibossSquad Numbers]] [[HollywoodCyborg Cyborgs]] was revealed in [[AllThereInTheManual the Sound Stages]] to have been created by [[BigBad Jail]] through cloning, using his own genetic material.
* ''Manga/RanmaOneHalf'':
** After accidentally looking into an enchanted mirror, Ranma creates a copy of his female self. She is, of course, infatuated with him. In the end, he accidentally copies his male self and the two copies fall for each other.
** In an anime episode, [[DirtyOldMan Happosai]] attempts to split Ranma into male and female forms for his own selfish reasons. However, he misses the fact that, A: Ranma despises him and would never obey him no matter the form, and B: the incense he uses is called [[LiteralSplitPersonality Personality Splitter]]. The result is an EnemyWithout situation, as the Clone is a vampiric ghost-like entity that is the living embodiment of Ranma's evil.
* The title character of ''Manga/BattleAngelAlita: Last Order'' and several others are cyborg clones of the same woman. Sechs was original female, but decides to alter their body to be male.
* The female Nemu is the clone of the male Mayuri Kurotsuchi in ''Manga/{{Bleach}}''. It's more apparent when Mayuri doesn't have his make-up on.
* In the ''Anime/StarshipGirlYamamotoYohko'' TV series, Yohko's male clone, Yousuke appears. [[spoiler:He turns out to be female and in disguise, but then actually becomes male later.]]
* ''[[Manga/GetterRobo Getter Robo Armaggedon]]'': [[spoiler:the pilot Go]] is a male ArtificialHuman made from the DNA of [[spoiler:Dr. Saotome's deceased daughter Michiru]].
* In the {{hentai}} {{manga}} ''Alice's Adventures in Sexland'', obviously an erotic retelling of Creator/LewisCarroll's book, there is a furniture maker whose furniture is special because it will spring to life and play with the user. Her "masterpiece" is a mirror, which sprung a magical clone of Alice, because, as the creator says, ''Who knows best what you like and what you need, [[ScrewYourself if not yourself]]''. Only it wasn't [[{{Hermaphrodite}} an exact clone]]. She does state, however, that the mirror would have given a perfect clone if that was what Alice had wanted.
* The former trope picture is from the artist Pisipisi and is of a series of drawings where a perverted MadScientist college student clones the girl he's stalking -- but puts his own mind in the clone instead of her's. Of course, then "she" wakes up, realizes that "she" now has a horny pervert expecting "her" to be a personal sex toy for him and his friends, and is less than enthused about the idea. Then she discovers that his male mind has [[OhCrap no defense]] against [[ManIFeelLikeAWoman female erogenous zones]]... Unfortunately, Pisipisi banned all American visitors to his website years ago due to harassment, and (possibly due to rumors of "Pisipisi" being the pen name of professional artist Saiwase Okiba) [[ScheduleSlip frequently goes months or years without updates]].
* Keith Violet of ''Manga/ProjectARMS'', the one female amongst the many clones of Keith.
* ''Anime/YuGiOh'', it isn't a clone exactly, but the digital copy of Mokuba Kaiba that Seto Kaiba creates in a video game is ''Princess'' Adina. It makes a bit more sense in the Japanese version, where the Big 5 programmed the female Mokuba rather than Kaiba.
* The Innovators in ''Anime/MobileSuitGundam00''. They are all technically genderless but come in pairs; out of the four pairs only one has both members having adopted a persona of the same gender (both male), so each of the other six Innovators has their own opposite sex clone of sorts.
* One of Manga/{{Naruto}}'s techniques involves either transforming into and occasionally summoning several more, scantily clad female versions of himself. And by "scantily clad", we mean "[[CensorSteam clothed in convenient smoke]]".
* ''Anime/DarkerThanBlack'' Gemini of the Meteor reveals that [[spoiler:Suou is an opposite-sex clone that Shion created of himself to pass as his twin sister complete with FakeMemories. The real Suou died during an unrelated assassination attempt]].
* ''Manga/KnightsOfSidonia'': Norio Kunato's "sister" Mozuku is in fact a clone of himself, but altered to be female. She takes care of all of Norio's responsibilities as head of the Kunato family while he devotes his time to piloting, and is mostly just seen as an extension of Norio himself.
* ''Manga/AyakashiTriangle'': About half a year after Matsuri [[GenderBender was turned from a boy into a girl]], exposure to TheCorruption somehow resulted in both a male and female Matsuri, each with all of Matsuri's previous memories and personality. Because only the girl has the mark of the initial transformation, it's judged most likely [[BornOfMagic the spell was turned]] into an [[OurSpiritsAreDifferent ayakashi]] with a copy of Matsuri's mind, turning the original back into a boy. However, it's entirely possible the boy is a clone, and thus an inversion.
[[/folder]]

[[folder:Comic Books]]
* ''Franchise/TheDCU'':
** In ''ComicBook/DCComicsBombshells'', [[spoiler:Superman]] is an opposite-sex clone of [[spoiler:Supergirl]], created by [[spoiler:Hugo Strange]]. He also created a same-sex clone, who is that continuity's [[spoiler:Power Girl]].
** Used in a ''ComicBook/Supergirl1972'' comic in which a criminal tries to clone ComicBook/{{Supergirl}} in order to use her twin to commit crimes. The (male) twin ends up committing suicide due to an Ethical Conflict between what his criminal creator has told him to do and what his twin wants him to do.
** ''ComicBook/WonderWoman1987'': Kreel biology means that attempting to clone a man may result in a female infant, as is the case for [[spoiler:the Emperor]].
* One ''ComicBook/JudgeDredd'' story set during a flashback to Chaos Day portrays a group of cadets trying to survive. One of them is stated to be a Dredd clone. Falcone is the obvious, but wrong, candidate. It turns out that the clone is female [[spoiler:and pregnant]].
* ''Franchise/MarvelUniverse'':
** TheCollector (whose hobby with species is exactly what his name suggests) once captures both ComicBook/HowardTheDuck and [[ComicBook/GuardiansOfTheGalaxy Rocket Raccoon]], and proceeds to create female clones of both, named Linda and Shocket, respectively... for [[ScrewYourself a breeding program]]. Both manage to survive and become their own individuals. (Incidentally, this suggests that the Collector isn't as good at his job as he seems, since while there's only one of Howard's species in their universe, there ''is'' a female Halfworlder raccoon out there named Captain Sale.)
** In Marvel Graphic Novel #72, ''Fear Itself'', featuring ComicBook/SpiderMan and Silver Sable (released in 1992), Silver Sable is after a mysterious European personage named "The Baroness", a white woman with a large '80s blond hairdo. During the climactic confrontation, the Baroness reveals herself to be [[spoiler:the previous Baron Zemo, cloned by Arnim Zola into a female body, and the hairdo is a large blond wig. She then takes off the wig and puts on the mask Zemo's father wore during World War II]].
** In ''ComicBook/SpiderGwen'', the Falcon is the teenage, male clone of ComicBook/CaptainAmerica, who is a black woman named Samantha Wilson in this continuity.
** Spider-Woman (who later went by Black Widow) in ''ComicBook/UltimateSpiderMan2000''. Ultimate Spider-Woman is a clone of Peter Parker, with all of his memories and personality traits -- except she's considerably more abrasive, especially to Miles. (They were going to blank her memory and give her an entirely new made-up personality and set of memories, but didn't get around to it before wackiness ensued.)
** ''ComicBook/UncannyXForce'': After Fantomex died, he was going to get a cloned body to come back with. However, he has three different brains, and each brain received its own cloned body. The nicest brain got a female body called Cluster.
** ComicBook/{{X 23}} is an Opposite-Sex Clone of ComicBook/{{Wolverine}}, developed by Dr. Sarah Kinney as a solution to not being able to recreate a Y chromosome from a sample of Wolverine's DNA. So instead, she doubled the X chromosome.
** ''ComicBook/XCellent'' introduces Phatty, a female clone of Phat, one of the dead members of ''ComicBook/XStatix''.
[[/folder]]

[[folder:Fan Works]]
* ''Manga/{{Naruto}}'' fanfictions happen to do this a lot to the main character, due to his Opposite Sex Clone actually being real. This is strengthened in the fact that it's not really a clone, but a disguise that quite literally changes Naruto into a girl. He can do this to his clones making literal Opposite Sex Clones.
* ''[[Creator/DetsniyOffSkiword Kid Icarus Uprising 2: Hades Revenge]]'' has the main character, Cloud Angle, create one, named Azul. Her first scene involves [[ScrewYourself her and Cloud]] [[IKEAErotica as a plan]] to avoid becoming {{virgin sacrifice}}s.
* ''Fanfic/TheBridge'': As a visual gag. Changelings can only mimic ponies of the same sex, so when a female one tries to mimic the transformed kaiju-to-unicorn Godzilla Junior the result is a female version of Junior.
* ''Fanfic/HeroTheGuardianSmurf'': Wonder Smurfette is one of the series' main protagonist Hero Smurf, who was created with the magical Mirror of Opposition when lightning struck it, thus creating a second adult female Smurf in the village besides Smurfette.
* ''Fanfic/SunsplitSaga'': Basically the core idea of the saga, and a FirstEpisodeTwist, [[spoiler:Sunset Shimmer]] is [[spoiler:Sunburst]]'s magical construct clone, whom he made to take on a date.
[[/folder]]

[[folder:Film -- Animated]]
* ''WesternAnimation/TheNightmareBeforeChristmas'': Dr. Finklestein builds a female version of himself out of body parts around his lab. While not a genetic clone, he does give her a half of his brain.
-->'''Dr. Finklestein:''' We'll have conversations WORTH having.
[[/folder]]

[[folder:Film -- Live-Action]]
* ''Film/{{Logan}}'': Laura was created from samples of Wolverine's blood, doubling the X-chromosome.
[[/folder]]

[[folder:Literature]]
* ''TabletopGame/BattleTech'' [[Franchise/BattleTechExpandedUniverse Expanded Universe]]: The Clans are a race of supermen and women that are mass-produced in batches from the DNA of their greatest warriors. During their childhood, all the clones engage in casual sex with each other to build bonds. This sometimes continues into adulthood, such as the Pryde siblings from the novel ''Legend of the Jade Phoenix'' and the Hazen siblings from the ''Mechwarrior: The Dark Age'' novels; both from Clan Jade Falcon. Maeve Wolf, of the Wolf's Dragoons, is secretly a sex-swapped clone of Jaime Wolf.
* ''Literature/BitingTheSun'': The heroine goes male so as to be able to produce sperm which s/he stores, then uses (once she switches back to female) to impregnate herself. It doesn't end up that well...
* In "Literature/ACloneAtLast", a short story by Bill Pronzini and Barry N. Malzberg, a man commissions a female clone of himself because he has no luck with women.
* ''Franchise/{{Dune}}'':
** ''Literature/GodEmperorOfDune'': Leto is introduced to Hwi Noree, the new Ixian Ambassador, who turns out to be [[spoiler:an Opposite Sex Clone of the previous ambassador, genetically tweaked to be irresistible to the inhuman Leto]].
** ''Literature/DuneEncyclopedia'' provides a history of all of Leto II's Duncan Idaho gholas, it includes that the Tleilaxu once provided a female Duncan Idaho ghola in a similar ploy to what the Ixians attempted with Hwi.
* ''Literature/ForgesOfMars'': The scientist Linya Tychon is a female clone of her [[TrulySingleParent father]] Vitali Tychon. He’d intended her to be male like himself, a carbon copy existing solely to carry on his work when he died, but an error in the creation process altered her sex and inspired him to treat her like a daughter instead.
* ''Literature/{{Genome}}'': In ''Dances on the Snow'', a genius geneticist named Edward Garlitski manages to create a female clone of himself named Ada Snezhinskaya. She shares his ambition and a vision of a world where genetic engineering is the norm. However, they have different ideas on how to achieve this goal. He chooses to work within the system by perfecting genetic engineering (in another book he is hailed as the father of genetic engineering). She decides that the entire society needs to be remade through political means. She creates hundreds of clones of herself and Edward and sends them out throughout TheEmpire to be adopted by unsuspecting parents. She then plots a rebellion, which would result in her ruling with her clones. Her plan fails but Garlitski's succeeds.
* In ''Literature/HouseOfSuns'', by Creator/AlastairReynolds, the various posthuman clone houses, such as Gentian Line, consist of male and female clones of a single individual.
* ''Literature/{{Interworld}}'': Joseph Harker, after accidentally traveling between dimensions for the first time, realizes that he has been replaced in this dimension by Josephine Harker, an almost-exact female copy of himself.
* ''Literature/NineLives'' by Creator/UrsulaKLeGuin: From 1968, it's possibly the oldest example in modern SF. Earth is in a sorry state, most people suffer from inborn defects. To remedy the situation, the best people are cloned. Usually, the donors are male, since it allows to easily clone both sexes, and mixed-sex groups of clones are proven to function better.[[note]]Women are sterile, which means even the second X chromosome wasn't added. No other symptoms of Turner syndrome are mentioned. Or maybe they are present, [[FridgeBrilliance but don't stand out against the background of other sick people]].[[/note]] The story explores the reaction of normal humans who have to work with a "ten-clone" created from a genius who died young. [[spoiler:And then how the sole survivor reacts to the death of his 9 siblings.]] Among other things it's mentioned that clones routinely share sleeping bags and sex seems just as natural for them as breathing. [[spoiler:An observer wonders if it's sex or masturbation.]]
* ''Literature/{{Otherland}}'': The CorruptCorporateExecutive Felix Jongleur's daughter is a clone of himself. [[spoiler:She's actually a byproduct one of his early attempts to make himself immortal. The idea was to have a clone of himself implanted in a surrogate mother. Of course, the child would need a similar upbringing to his own to produce an effective copy. To this end, Jongleur wanted the surrogate mother to be like his own mother. Unfortunately, he couldn't bring himself to unearth her body... so instead, he made an Opposite-Sex Clone of himself. That's right: he intended to impregnate an opposite-sex version of himself... with himself. Very {{Squick}}. Fortunately, the project never went beyond the initial stages because a far more appealing (yet no less unethical) option presented itself, so Jongleur adopted the Opposite Sex Clone for his daughter.]]
* After being murdered several times, the protagonist of ''Literature/ThePhantomOfKansas'' eventually [[spoiler:hooks up with her murderer (an illegal clone of herself that has undergone a sex-change) and buys a spaceship to head out for the most remote settlement in the solar system]].
* In James Patrick Kelly's story "Literature/{{Solstice}}," one of the main characters has himself cloned as a woman. [[spoiler:Later he turns it into ScrewYourself. He eventually has a moral epiphany and is filled with guilt, both about the incest and his selfishness in cloning himself.]]
* The ''Literature/{{Stardoc}}'' series has for a main character Cherijo Grey Veil, who is introduced as the daughter of [[VillainWithGoodPublicity renowned geneticist]] Dr. Joseph Grey Veil. [[spoiler:Later in the series, we discover not only is she a female clone of the good doctor, she was [[ParentalIncest created as a wife for him]]. Basically, he was [[{{Ubermensch}} such an egomaniac]] that he felt only his DistaffCounterpart would be a worthy mate. And he was willing to get laws passed declaring her a non-person in order to make sure ''she'' had no say in the matter.]]
* The character of Lazarus Long in Creator/RobertAHeinlein's book ''Literature/TimeEnoughForLove'' is the world's oldest living human. At the beginning of the book, he is trying to kill himself by avoiding "rejuvenation therapy", and a squad of his descendants rescues him. He makes a deal with them: he will go through rejuvenation therapy if they can unearth one experience he could do that he hadn't done already. Two of his female descendants arrange for Opposite Sex Clones of him to be implanted in them; both are born, and he finds himself raising twin female versions of himself.
* Creator/LoisMcMasterBujold's ''Literature/VorkosiganSaga'':
** Not quite the same, but it's stated in various novels that opposite-sex clones are possible, and that the corrupt rich people who choose to have their brains transferred into a younger clone sometimes pick this option (with optional plastic surgery beforehand, to make sure it's a ''pretty'' clone).
** It's implied in ''Literature/MirrorDance'' that some people order clones of themselves chiefly for the purposes of sexual experimentation, although the opposite-sex option isn't brought up at that point. It's also stated outright that the mass-produced Durona Group are of both sexes, although there seem to be more females than males. (The original is a woman.)
** In ''Literature/ACivilCampaign'', Lady Donna Vorrutyer becomes Lord Dono thanks to a cloned set of male anatomy. The missing Y chromosome was supplied by her dead brother. [[spoiler:Ivan gets really squicked by all this because he had previously had an affair with Lady Donna.]]
[[/folder]]

[[folder:Live-Action TV]]
* ''Series/DoctorWho'': In [[Recap/DoctorWhoS30E6TheDoctorsDaughter "The Doctor's Daughter"]], a female TrulySingleParent-offspring is made of the Tenth Doctor. She's a genetic duplicate made of a tissue sample, entirely against his will. The relationship between them is presented as father/daughter throughout, as she was created from splitting his chromosomes then randomly recombining them — like sexual reproduction, but him being both parents. The Doctor calls her a "generated anomaly". Donna shortens that to "Jenny". [[note]]The [[Creator/GeorgiaMoffett actress]] that played Jenny is the daughter of the [[Creator/PeterDavison actor]] who played the Fifth Doctor, and later married the [[Creator/DavidTennant actor]] who played the Tenth Doctor. So she's now a Doctor's daughter who played the Doctor's daughter and then had the Doctor's daughter. Gallifreyan DNA is weird.[[/note]]
* ''Series/OrphanBlack'' focuses on several clone characters played by Creator/TatianaMaslany. One of them, introduced in Episode 8 of Season 2, is a trans man named Tony (formerly Antoinette). [[spoiler:In Season 3, the focus is on Project CASTOR, which involves a series of male clones that are genetic siblings to the female clones. In fact, both lines come from a single donor, who happens to be a genetic chimera, having absorbed her male twin brother in the womb and thus has two sets of DNA.]]
* ''Series/RedDwarf'':
** "[[Recap/RedDwarfSeasonIIParallelUniverse Parallel Universe]]" has opposite-sex versions of the whole crew, where Rimmer's female double (AlternateUniverse rather than cloned) comes on to him in a spectacularly unsubtle way, and where Lister proceeds to ''[[MisterSeahorse sleep with and get pregnant by his female counterpart]]''. This episode also features an obvious joke:
--->'''Arlene Rimmer:''' ''[to Lister]'' What could possibly have made you contemplate... making love to yourself?\\
'''Arnold Rimmer:''' Well, why break a habit of a lifetime?
** "[[Recap/RedDwarfSeasonVIRimmerworld Rimmerworld]]" has Rimmer try to go the pervy route with this when he winds up stranded on an unoccupied planet. He screws up the cloning, though... several times. Even when he gets it "right", the clone still has his (male) face, but by that time, he's a prisoner of his own male clones and thus has bigger problems...
--->'''Rimmer:''' Technically, she would be my sister, and therefore unable to take me as her lover. After much soul searching, I reluctantly decided, "What the hell", I just wouldn't tell her.
* ''Series/{{Sliders}}'':
** An episode has Quinn meet Logan, his DistaffCounterpart. Turns she actually ''is'' him--in this universe Quinn was born a girl. [[spoiler:She's also an EvilTwin.]]
** In another episode Quinn is on a talk show and mentions meeting Logan, and of course the host immediately asks if [[ScrewYourself he slept with her]]. They did kiss, though for his part Quinn was unaware of the connection at the time.
** It's interesting that Logan found out that Quinn is her double via a "thermal scan", basically, an advanced biometric system that maps each person's unique "heat signature". The FridgeLogic comes from a man and a woman having the same heat signature, even if they do share the same parents.
* ''Series/StarTrekTheNextGeneration'': When a non-corporeal entity impregnates Troi in "[[Recap/StarTrekTheNextGenerationS2E1TheChild The Child]]", the baby is half-Betazoid and half-human, just like its mother in every way, except male.
[[/folder]]

[[folder:Music]]
* ''The Clone Song:'' words by Creator/IsaacAsimov, sung to the tune of ''Home on the Range''. Read the full version [[http://members.tripod.com/~bardic_circle/aclone.htm here]].
-->Clone, clone of my own,\\
With its Y chromosome changed to X.\\
And when I'm alone\\
With my own little clone\\
We will both think of nothing but sex.
* Cartoon band Music/ThePlasticEGulls have Tina the bass player, a clone of Ian the frontman, made to satisfy the record company's [[ExecutiveMeddling mandatory bass player clause]]. Ian would happily label her as a DistaffCounterpart if her {{Cloudcuckoolander}} persona wasn't in the way.
-->'''Ian:''' ''([[http://fav.me/dczlstx sourced]] from CharacterBlog)'' Tina says hi. She also says that she sniffed her bedsheets and [[TooMuchInformation they smell like her]]...Ugh, that is ''so'' un-me.
[[/folder]]

[[folder:Mythology and Religion]]
* In many Mesoamerican and Southwestern Native American cultures, the gods often come in male-female pairs. Whether they're intended to be different aspects of the same god, brother and sister, husband and wife, or this trope is not always clear.
* In Literature/TheBible, it is said that Eve was created out of Adam's rib, making her for all intents and purposes one of these.
[[/folder]]

[[folder:Tabletop Games]]
* ''TabletopGame/BattleTech'' General Maeve Wolf of [[PrivateMilitaryContractors Wolf's Dragoons]] is a female clone of founder Jaime Wolf. Rumors had it she was his illegitimate daughter even though public records claimed her as one of the war orphans the company took in while their own records had her as one of their [[DesignerBabies Trueborn]] warrior of such mixed heritage that she refused a proper Bloodname.
* ''{{TabletopGame/Traveller}}'': Archduke Norris Aella Aledon never married, but needed an heir, so he had a daughter (Seldrian Aledon) created. Cloning to have children happens so often, there is a term specifically for clone offspring (especially ones created to provide an heir for nobles): "true-son" or "true-daughter".
[[/folder]]

[[folder:Video Games]]
* In ''VideoGame/BioShockInfinite'', [[spoiler:it turns out that Robert Lutece is not Rosalind's twin brother, but rather [[AlternateSelf an opposite-sex version of her from an alternate universe]]]].
* The ''VideoGame/CrusaderKingsII'' mod ''VideoGame/CrisisOfTheConfederation'', being a space opera mod, includes cloning as a possibility. Males cloning themselves can choose whether the clone will be male or female (females can only make female clones, though).
* The [[PurelyAestheticGender female version]] of Alex in ''VideoGame/DeusExInvisibleWar'' is a clone of Paul Denton.
* In ''VideoGame/Drakengard3'', each of the Intoners has their own Disciple to serve them as a companion, [[BodyguardingABadass bodyguard]], and [[ExtremeLibido to sate their ramped-up sexual needs.]] Instead of a normal Disciple, One somehow spontaneously created a male clone of herself, who she keeps hidden from the other Intoners as a secret weapon against Zero. He only appears in the main story at the very end of Branch A, as a DiabolusExMachina who {{backstab}}s Zero out of nowhere, but he co-stars in One's DLC missions, turns out to be the founder of the Cult of the Watchers that features so prominently in ''VideoGame/{{Drakengard}}'', and is the main character of the ''Drakengard 3'' spinoff manga. And yes, One used her brother to meet her sexual needs as well - she at least felt guilty and conflicted about it, he didn't see what the problem was. And no, he isn't given a name beyond One's Brother, and in Route A's epilogue is able to [[DudeLooksLikeALady pass himself off as One to take her place.]]
* A very important and hidden sidequest in ''VideoGame/EldenRing'' reveals that [[spoiler:Radagon is secretly Queen Marika's 'male half', with his own will and distinct personality but intrinsically connected to the God-Queen. Though Radagon's exact origins are unknown, it's implied that he physically split off from Marika at some point in the past, and that just before or right after the Shattering he fused back with Marika, as they presently share the same body, which shifts depending on who is in control. The two are actually remarkably similiar physically, with Radagon simply having a more obviously masculine build and red hair, while Marika has a more feminine (though still imposing) build and blond hair.]]
* In ''VideoGame/TheElderScrollsIIIMorrowind'', Divayth Fyr (a very powerful, [[ReallySevenHundredYearsOld very old]] wizard), made four female clones of himself: [[ThemeNaming Alfe, Beyte, Delte, and Uupse]]. They're variously described as [[ScrewYourself wives]] or daughters. Surprisingly this isn't particularly played for {{Squick}}... at least within his tower.
* ''VideoGame/EndlessSpace'' has the Horatio faction, an array of clones of ultra-rich Horatio the First. Some of them are female, because Horatio got bored with just his male clones.
* ''VideoGame/TheKingOfFighters'' series has Kula Diamond, one of the many clones of Kyo Kusanagi. In addition to the sex flip, Kyo's powers over [[PlayingWithFire fire]] became Kula's powers over ''[[AnIcePerson ice]]''.
** The series can be inconsistent about "clones," sometimes using the term to describe [[CloneByConversion unrelated people who have just been altered to be like someone else]], and not clones in the normal sense. This makes Kula's status as a clone unclear.
*** Kula did say in her storyline in ''KOF 2001'' that she was "grown from a petri dish" (at least in the English version, anyway), which would make her fall squarely into this trope.
*** [[FlipFlopOfGod However]], Kula's bio in ''Maximum Impact 2'' hints that she's a clone [[MixAndMatchMan in the "genetic manipulation" sense]] (i.e. a normal girl surgically implanted with Kusanagi DNA, much like K' and Nameless). While the ''MI'' series ''is'' an AlternateContinuity, most of the characters' backstories seem to be unchanged, so...
* In ''VideoGame/KingdomHearts358DaysOver2'', [[spoiler:Xion is a clone of [[TheHero Sora]] literally made out of memories.]] It's played with a bit, as what you see while looking at them varies from person to person (such as Xigbar seeing her as [[VideoGame/KingdomHeartsBirthBySleep Ventus]]), but the ShapeshifterDefaultForm is [[spoiler:Kairi with black hair.]]
* In ''VideoGame/MassEffect2'', Miranda Lawson is genetically engineered using her biological "father's" modified genome to be [[CursedWithAwesome perfect]]. Unsurprisingly she has some pretty severe daddy issues resulting from what she perceives as her father's egotistical arrogance. Ultimately by the end of ''2'' and in ''3'', they turn out to be two ''VERY'' different people, with Henry Lawson turning out to be even worse than Miranda described and Miranda showing herself to be a JerkWithAHeartOfGold, with the Jerk part eventually being shed.
* By the time of ''VideoGame/MegaManZero'', the four Guardians were created from VideoGame/MegaManX's DNA. One of these Guardians is the obviously female Fairy Leviathan.
* ''VideoGame/NintendoWars'': Caulder/Stolos from ''Advance Wars: Days of Ruin'' has his 'daughters', Opposite Sex Clones of himself. They don't look that much like each other, so he appears to have fussed about with their genetics a little (which could explain their different eye colors). He does have a male clone as well, Cyrus, but he plays little part in the story.
* In ''VideoGame/TheSims2'', a cheat can be used to impregnate a Sim by him/herself. Doing this will produce offspring that is a clone of the parent, but it will always be of the opposite sex. Notably, the child Brandi Broke is pregnant with when first played is such a clone, and isn't genetically related to his supposed father at all.
* In the ''Franchise/StreetFighter'' series, Cammy is a female clone of M. Bison (Vega in Japan, or simply "Dictator" in international discussions), meant to serve as one of numerous backup vessels for him due to the inevitable Psycho Power-induced burnout of his body. Also a case of [[MixAndMatchMan mix and match girl]], given how she's basically an identical clone of the kidnapped Russian girl named Decapre, with some Bison thrown in for maximum Psycho Power containing capability.
* ''VideoGame/{{Starfield}}:'' [[spoiler:Hadrian Sanon]] is the clone daughter of an executed war criminal, and is worried that her father's misdeeds will be used against her. In the conversation where we learn all of this you can reassure her that she is not her father; and when asked how an opposite sex clone is even possible, she will reply "A lot of gene editing."
* ''VideoGame/SuperRobotWars'':
** Ingram Prisken and Viletta Vadim were the male and female options for the protagonist of ''Super Hero Sakusen''. The ''Alpha'' and ''Original Generation'' games re-envision them as this trope, with Viletta being a female clone of Ingram who wouldn't be subjected to the same mind control that he was under.
** [[VideoGame/SuperRobotWarsW Kazuma Ardygun]] has a female clone named Aria Advance. Unlike most examples, she's not a ''genetic'' clone, but rather a digital being based off Kazuma's data.
* In ''VideoGame/{{Xenosaga}}'' URTV #668, named Citrine is the opposite sex clone of Dimitri Yuriev, as were all of the female variant [=URTVs=].
[[/folder]]

[[folder:Visual Novels]]
* ''Franchise/FateSeries'': A weird one. In real life Arthurian legend, Mordred is the illegitimate son of King Arthur, made through the intervention of Morgan le Fey (exactly what she did varies depending on the legend). In ''VisualNovel/FateStayNight'', one of the first major reveals is that [[spoiler:King Arthur [[HistoricalGenderFlip is actually female]]]]. Many fans assumed that Mordred would therefore be an Opposite-Sex Clone. In ''Literature/FateApocrypha'', we find out that Mordred is indeed an Opposite-Sex Clone... except [[spoiler:she's still female. Specifically, Artoria was [[GenderBender temporarily transformed into a pseudo-male]] by Merlin, and Artoria's sister Morgan le Fey used the opportunity to get a DNA sample. She then used that to grow a [[OurHomunculiAreDifferent homunculus]] of the opposite gender, meaning Mordred came out female]]. WordOfGod explained some of this before ''Apocrypha'' came out.
* Arcueid of ''VisualNovel/{{Tsukihime}}'' is more or less a female clone of Crimson Moon Brunestud, the UltimateLifeForm of the Moon and the original template of [[OurVampiresAreDifferent all Nasuverse vampires]]. She wasn't intentionally created as such, but as the most powerful True Ancestor to have ever existed, she is, per Nasuverse logic (that copies are always inferior to their originals), the closest it gets to a being a carbon copy of the original vampire, Crimson Moon (who was a guy).
* ''VisualNovel/MajikoiLoveMeSeriously'' has [[http://en.wikipedia.org/wiki/Minamoto_no_Yoshitsune Yoshitsune]] and [[http://en.wikipedia.org/wiki/Benkei Benkei,]] as per their namesakes, are the female clones of the famous historical figures. Seiso is likely this as well, though it's not yet revealed who she's a clone of.
[[/folder]]

[[folder:Webcomics]]
* ''Webcomic/ElGoonishShive'' had protagonist Elliot using an ancient magical artifact to try to undo a {{Magitek}} GenderBender, inadvertently creating Ellen in the process. She was "born" with a perfect copy of Elliot's memories and personality (though she says the memories feel more like stuff she learned, rather than experiences), but her initial CloneAngst and subsequent (successful) [[FreakOut attempts]] to establish her own identity led to DivergentCharacterEvolution, so she isn't a simple DistaffCounterpart. People calling her a clone (before getting to know her) is a ''huge'' BerserkButton. More than a few scenes make it clear that the classic sex partner idea seriously {{squick}}s both of them, since they ''genuinely'' care about each other like any two siblings would. Elliot in particular has gone on record saying that his ''first thought'' when he saw Ellen mid-[[FreakOut freakout]] was "She's family, help her.". Furthermore, since Ellen was split off by the [[GreenRocks Dewitchery Diamond]], Elliot can be considered Ellen's father and (since this is ''El Goonish Shive'' and a GenderBender was inevitably involved) her ''mother'' as well. Unfortunately, all of this (plus Elliot's ChronicHeroSyndrome and Ellen's initial CloneAngst) causes him to treat her like a ''[[BigBrotherInstinct little]]'' sister, which [[http://www.egscomics.com/?date=2007-06-02 tends to annoy her no end.]]
** This potentially may get even more complicated, now that [[spoiler:Magus (a AlternateUniverse version of Ellen who had used GenderBender magic in his home world[[note]] while he excuses this on some rather sketchy logic about physical strength, the evidence seems to point to this Ellen being UsefulNotes/{{Transgender}}, hence the switch[[/note]] before getting trapped in the EGS universe in a ghost-like state) is now walking around in a a ''male'' Dewitchery Diamond duplicate of Elliot's body. For now, Magus is avoiding the other two, but it is likely that he will re-appear eventually.]]
* The community-driven interactive fiction ''Webcomic/MSPaintAdventures'': ''Webcomic/ProblemSleuth'' introduces a trio of detectives: Problem Sleuth, Ace Dick, and Pickle Inspector ("pickle" in this context is meant to connote "problem"), who, through spirit quests, are psychically linked with female counterparts of themselves, whom they eventually meet in person and are instantly attracted to. Problem Sleuth gets Hysterical Dame, Pickle Inspector gets Nervous Broad, and Ace Dick, lacking the imagination to think of a female counterpart, simply becomes linked to another male version of himself (albeit one who owns a blonde wig).
* In ''Webcomic/DragonTails'', when Norman the squirrel orders a clone of himself, he marked the sex box with an F (for "Fine the way it is") and got a female clone. The clone is also a child, since this was done with genetics.
** They were also really drunk while designing her genetic pattern, so they made her glow-in-the-dark as well.
* ''Webcomic/TheInexplicableAdventuresOfBob:'' While trying to manipulate Galatea, Riboflavin shapeshifts into his idea of what a male of her species would look like. Unfortunately, she doesn't actually ''have'' a species, and her reaction is, "Oh my G...! A sex-swapped version of my own ''face?'' I'll have nightmares for a week!" He then disguises himself as a handsome human male, and gets a much more favorable reaction from her.
* Not an example, but in ''Webcomic/DumbingOfAge,'' Sarah refers to Joyce's nearly-identical older brother [[spoiler:actually sister]] Joshua as this.
* In ''Webcomic/IDontWantThisKindOfHero'', Tracy is this to Baek Morae, created by a group of scientists in an attempt to duplicate the latter's power of purification. Baek Morae, on his part, considers her existence disgraceful and so tries to kill her when they meet in the present.
* ''Webcomic/HolyBibble'' portrays [[Literature/TheBible Eve]] as this to Adam, since she is created from his rib. Due to the comic's simplistic art style, this is primarily shown by her having a similar curly forelock to him, as well as the same skin and hair colors.
* Subverted in ''Webcomic/SkinHorse''- when the characters travel to an alternate dimension, Tip discovers that this universe's version of him is a woman- but eventually they realize that although they were born at the same time from the same parents, they're no more genetically related than a different brother and sister.
[[/folder]]

[[folder:Web Original]]
* In ''WebVideo/TheGuild'' Bladezz' little sister looks even more like him than you'd expect (the androgynous clothes probably help). This isn't to put her down, if anything it makes Bladezz retro-actively prettier.
* As a corollary to {{Rule 34}}, {{Rule 63}} (it would have been Rule 36, but it's reversed, [[DontExplainTheJoke get it?]]) says for every male character, someone has made a female version of that character, and vice versa.
** Quoth many an anonymous users: "It's not sex; it's masturbation!"
* The web-toon series ''WebAnimation/BonusStage'' featured a female clone of main character Phil Argus as a [[OneSceneWonder gag character]], once as a "[[RelationshipSue perfect girlfriend]]", the next as a "[[ShooOutTheNewGuy cheap ploy for additional viewership]]" (applified by [[PlayboyBunny bunny ears]] and [[UsefulNotes/FurryFandom fox ears]] (presumably to appeal to a wider spectrum of viewers).
* A variant from the Literature/WhateleyUniverse: In "The Second Book of Jobe", kleptomaniac devisor Belphegor ends up downloading a copy of his own personality into a cloned female drow body based on Jobe's "drow formula" and DNA. Hilarity quite naturally ensues, though the overall awkwardness of the situation (and not just because campus security shows up right the next moment) isn't neglected -- for one, both Belphegor and Jobe find themselves promptly declared "Belphoebe"'s ''parents'' of record, and there's every reason to believe that she's here to stay because there's in-universe evidence that she's managed to dodge the CloneDegeneration bullet...
[[/folder]]

[[folder:Western Animation]]
* ''WesternAnimation/BionicSix'': Dr. Scarab's attempt to create a female companion resulted in this. Later, his female clone created opposite sex clones of his henchmen.
* Danielle "Dani" Phantom in ''WesternAnimation/DannyPhantom''. She and Danny continue to call each other "cousins" even after she reveals the truth about what she is.
* ''WesternAnimation/DuckTales2017'': The finale shows [[spoiler:Webby]] is some kind of artificial daughter to [[spoiler:Scrooge [=McDuck=]]], which Bradford made because retrieving an ArtifactOfDoom required a direct descendant of the latter. She's not quite called a "clone", or identical-looking, but no mention is made of adding any other person's DNA. [[spoiler:May and June are also genetically Scrooge's daughters, although they were actually made by cloning Webby via magic. Scrooge, for his part, after his initial shock at being called Webby's dad, is elated to find out they're biologically related.]]
* In a Valentines episode of ''WesternAnimation/HouseOfMouse'', Ludwig Von Drake deduces that the perfect partner for someone would be an Opposite Sex Clone. The two can't stand each other, and she breaks up with himself.
* In ''WesternAnimation/InfinityTrain'', it's revealed that [[spoiler:Hazel, a six-year-old girl]] is a failed clone of [[spoiler:Alrick, Amelia's deceased husband]].
* ''WesternAnimation/JohnnyTest'' has at least three female doppelgangers of Johnny. Princess Maribel is by far the most exact and doesn't get an upgrade in hairstyle or even a clothing change aside from a little lipstick. Sissy is the second and Joni West is the last.
* ''WesternAnimation/ThePowerpuffGirls1998'': The Rowdyruff Boys, in a twisted-EvilCounterpart way. They aren't so much clones as [[EvilKnockoff bargain-basement knockoffs]], made when Mojo Jojo imitated the Powerpuffs' creation using more "manly" ingredients. Despite the resemblance to their counterparts, no one treats them as genetically related. The girls defeat the boys [[GirlsHaveCooties by exploiting their disgust for romantic interest]] without any sort of incestuous implications.
* ''WesternAnimation/StarWarsTheBadBatch'' has introduced a single female clone of the very masculine Jango Fett: [[spoiler:Omega]], who is not under the control of Order 66 and has joined the Bad Batch. The season two finale introduced another female clone in [[spoiler:Dr. Emerie Karr]] who works for the Empire.
* ''WesternAnimation/TransformersAnimated'':
** Each of Starscream's clones [[LiteralSplitPersonality represents]] some [[DirtyCoward facet]] [[SmallNameBigEgo of]] [[SycophanticServant his]] [[BadLiar personality]], which includes a female one (retroactively named Slipstream)--who [[YouDoNotWantToKnow refuses to explain what part of his personality she represents]]. [[EpilepticTrees Fan theories]] range from his ambition, to his feminine side, to his opportunism, to his [[TheStarscream treachery]], to his... ah, ''[[HoYay feelings]]'' for Megatron. WordOfGod has deemed it a RiddleForTheAges, and won't even say if Slipstream herself knows.
** [[spoiler:Sari Sumdac]] seems to be a partial example: She is an {{organic|technology}} [[MechanicalLifeforms Cybertroninan]], but looks like the daughter of [[spoiler:her human "father" Isaac Sumdac]]. Even besides sexual characteristics, the resemblance is far from identical. [[spoiler:They share skintone, but not hair or eye color]]. It's not clear if this was because her protoform was designated to be female, or it just randomly became such.
* X-23, a female clone of Wolverine, and an eventual CanonImmigrant from ''WesternAnimation/XMenEvolution'' to the comics. See the entry under ComicBooks for more detail.
[[/folder]]

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