->''"That boy has a really high-pitched voice."''
-->-- '''[[Creator/BenCroshaw Ben "Yahtzee" Croshaw]]''' during a crossover appearance on ''WebVideo/{{Unskippable}} - VideoGame/StarOceanTheLastHope''

An unfeminine female character looks like, or is mistaken for, a male. Sometimes this is to show that she's as tough as a guy, even OneOfTheBoys, sometimes to show that she's a ButchLesbian, but frequently just to show that she's unattractive. Often used for comedy, in which case she may be an AbhorrentAdmirer.

Supertrope of {{Bifauxnen}}, where a woman looks like an ''attractive'' young man, usually with fangirls (or fanboys) who take obvious notice. Not to be confused with SweetPollyOliver, who is a woman ''deliberately disguised'' as a man; SamusIsAGirl, where the story is deliberately misleading about the character's gender; or ViewerGenderConfusion, where there's only out-of-universe confusion. Compare DudeLooksLikeALady and AttractiveBentGender, for men who look like women, and TheLadette, for women who ''act'' like men. Sometimes involves a GenderReveal.


[[folder: Anime and Manga]]
* Nagi Ichinose from ''Manga/NyanKoi'' is an interesting case. She sounds like a gruff-voiced woman, but most assume her to just be a feminine-sounding man. So much so that every guy she attempted to flirt with turned her down, saying they didn't "swing that way." She eventually moved on to chasing girls, not because she turned lesbian, but because she wishes to "chase the beauty that [she] so wishes to have." She eventually falls in love with Junpei, after he tells her that he doesn't care that she looks like a boy, and she defaults to chasing after him.
* ''Anime/ProjectAko'' :
** ''Project Ako'' has a female OneGenderRace of HumanAliens. Many look like guys.
** And let's not forget Mari. Or as she is sometime known, "Mari of the [[Manga/FistOfTheNorthStar North Star]]".
* In ''Manga/CafeKichijojiDe'', Jun has a bunch of macho underlings who resemble gorillas. His co-workers were quite shocked when he tells them that they're all girls.
* ''Anime/CowboyBebop'':
** Ed is a girl, which the rest of the cast doesn't realize until the very end of the episode in which she's introduced. Given that she's young enough to be prepubescent and her personality is halfway between FeralChild and {{Cloudcuckoolander}} anyway, it doesn't actually affect her characterization much.
** VT the middle-aged space trucker.
* ''Anime/MobileSuitGundamSEED'': Cagalli is introduced this way, though she wasn't ''specifically'' disguising herself as male. People assume she is until it's revealed otherwise, save for Kira who easily figures it out.
* ''Manga/{{Hellsing}}'':
** Sir Integra, who dresses in a masculine fashion and is referred to as "sir" despite being female. It's explained that this is deliberate on her part, to make people more accepting of her taking the traditionally male role of the head of the Hellsing Organization. Nevertheless, this wasn't as apparent at the beginning of the manga, due the fact that she had an impressive bust, but started to shrink in later parts of the manga, and became small chested.
** Zorin Blitz is an even more convincing example, despite having the BoobsOfSteel.
** Heinekel Wolfe is too.
* ''Anime/MagicalGirlLyricalNanohaStrikers'': The supplementary manga revealed that Otto of the Numbers Cyborgs had such a boyish appearance that most of her own {{Cyborg}} family were unsure if she had a male or female body.
* ''Manga/OuranHighSchoolHostClub'':
** Very early on in ''Ouran High School Host Club'', where Haruhi is assumed to be male because of her very unflattering appearance. This continues through the rest of the series, though a proper clothing upgrade makes her into more of a {{bifauxnen}}.
** Under similar conditions, Benibara of Lobelia has a rather masculine face despite dressing more effeminately. ''WebVideo/OuranTheVaguelyAbridgedSeries'' parodied this by giving her a craggy man's voice.
* ''Anime/HeartcatchPrettyCure'': Both Tsubomi and Cobraja thought that Itsuki was a male until different circumstances revealed the truth (a servant of Itsuki's called her "Oujo-sama" in front of Tsubomi and Erika/Cure Marine told Cobraja flat out that Itsuki was a girl). Cobraja was shocked while Tsubomi ended up falling slightly ill from the revelation.
* ''Webcomic/AxisPowersHetalia'':
** [[ProperLady Liechtenstein]] [[ImportantHairCut cuts her hair short]] to mimic her adopted brother Switzerland. When the two go walking while wearing the same military uniform, an old lady compliments Switzerland on having an adorable younger brother. [[ACupAngst Cue Liechtenstein groping her chest in shock.]] This isn't as much of a problem when she wears dresses and [[HairDecorations a ribbon.]]
** Hungary thought she was a boy, and so did Prussia until he realised she had breasts. However, she looks a lot more female after puberty, when she has longer hair and is often seen wearing dresses.
* When Sechs from ''Manga/{{Gunnm}}'' [[RunningGag still had]] her ([[GenderBender female]]) TUNED body, it was easily masculine enough to be confused for male, especially considering the manga's artstyle. Compare and contract with the twins, who, despite having the same series TUNED bodies, are easily identified as females.
* ''Manga/WaratteSotomuraSan'': In a flashback from when the Sotomura siblings went to a store to buy yukatas, each one was given something for the other's gender.
* Honey's and Natsuko's classmates in the original ''Anime/CuteyHoney'' manga, who are all [[{{Gonk}} ridiculously ugly]].
* In ''Manga/GunsmithCats'' we have the psychotic Russian assassin Natasha Radinov she looks like a tall broad shouldered young man, she covers her rather large breasts with her trench coat most of the time.
* Both Tohru and Ilulu mistook Kobayashi for a man when they first met her in ''Manga/MissKobayashisDragonMaid''. This is helped by her wardrobe containing a grand total of two feminine outfits (a women's kimono and a maid outfit), neither of which she ever wears during the course of the anime (she does wear the maid outfit once in the manga, but she had to pretty much be forced into it). She even undergoes a GenderBender at one point, and absolutely nothing about her outwards appearance changes.
* Kuu from ''Anime/HaibaneRenmei'' is a young girl with short hair who wears unisex clothes. She's mistaken for a boy by the man she works for but she doesn't feel the need to correct him.

[[folder: Fan Works]]
* Charlie, the resident fox of Fanfic/PackStreet, has the physique of a guy, dresses like one, and she doesn't act exactly feminine. [[spoiler:None of that stops the ShipTease between her and Remmy.]]

[[folder: Film -- Animation]]
* In ''WesternAnimation/StrangeMagic'' one of the Bog Kings [[ThoseTwoGuys minions]] is revealed to be a female, much to the surprise of the other minion who had bumbled through the movie with her.
* [[MeaningfulName Madame Gasket]] in ''WesternAnimation/{{Robots}}'' is mistaken for a man, even down to the voice. TheReveal that she is in fact a woman has some pretty hilarious reactions (including the glass that helps protect the eyes shattering).
* Done in ''WesternAnimation/TheLorax''. The title character is about to open a can of fury on a relative of The Onceler's, who objects saying, "You wouldn't hit a woman, would you?" "That's a woman?!?!!?!?"
* ''WesternAnimation/KungFuPanda'' has Master Tigress who, unlike Master Viper, lacks TertiarySexualCharacteristics to indicate any femininity (even her voice actress was initially confused), in addition to a low voice. Taken UpToEleven in the animated series, where her being mistaken for a man (despite her name obviously indicating otherwise) is a RunningGag and she is often subject to FemininityFailure.
* ''WesternAnimation/{{Shrek}}'' has Doris, one of Cinderella's ugly stepsisters. Emphasis on ''ugly''. She can easily be mistaken by a man on a drag and has an amazingly deep voice. Fiona even once remarks "You go...! Girl...?"

[[folder: Film -- Live-Action]]
* In ''Film/SkyHigh2005'', Royal Pain is thought to be a guy because of the voice modulator. It is later revealed that she is a she when she finally puts her evil plan into action.
* Executed brilliantly in ''Film/TheDarkKnightRises'' whereby [[spoiler:flashbacks are shown of a child with a shaved head and modest dress. We're told this was the child of Ra's al Ghul and Bruce believes it is Bane, but it turns out to be Miranda Tate, birth name Talia]].
* One of the most bizarre recurring gags in the ''Film/{{Airplane}}''-style spoof film ''Film/HotShots'' concerns Kowalski, a pilot who is considered by all the other pilots (including the film's [[IdiotHero protagonist]]) and even her drill sergeant to be a man, even though she's obviously a woman, and a pretty one too. Her feminine appearance, her feminine voice, and the fact that she wears a bra during roll call while all her fellow pilots are barechested are still not enough to convince anyone due to RuleOfFunny; everyone assumes she's just a small man who has trouble sucking in his chest. (Of course, it doesn't help that throughout the movie she is [[LastNameBasis always referred to by her gender-neutral surname]].) Maybe this is supposed to be a mockery of PoliticalCorrectnessGoneMad, with Kowalski being made to feel not just equal, but completely indistinguishable from the men. If so, it's not really necessary, since, due to the nature of this film series, Kowalski is just as competent - which is to say, incompetent - as all the other characters.
* ''Film/RomeoMustDie'' has a scene that combines this trope with SuddenlyEthnicity. Han (Creator/JetLi) is attacked by a faceless biker who looks reasonably masculine in appearance and who is presumably black (Han's Chinese family has been fighting with the black gangs in Oakland). Once he's chased the biker down, the biker takes off "his" helmet and exposes "himself" as a Chinese woman. It signals a major turning point in the plot, since for the rest of the movie Han is battling a ''rival'' Chinese gang.
* One appears in ''Film/RatRace'':
-->'''Owen''': (''talks to a bartender who stands with her back to him'') Miss? Could I have another one of these, please?
-->'''Bartender''': (''turns around'')
-->'''Owen''': (''sees her maskuline face'') Oh I'm sorry, I thought you were a woman!
-->'''Bartender''': I ''am'' a woman!
* In ''Film/{{Bound 1996}}'', Caesar walks in on his girlfriend having an intimate conversation with a ButchLesbian, Corky. He's about to get angry when Corky turns around and reveals herself to be a woman. Caesar excuses himself and mutters about how dark the room is. Corky was probably written as being more butch then she ended up, as it's not particularly believable to mistake her for a man.
* One of the detectives' "solutions" to the mystery in ''Film/MurderByDeath'' has the supposed butler being a woman.
* The plot of ''Film/{{Tomboy}}'' is about a ten year old girl who moves to a town and decides to [[SweetPollyOliver try and pass as male]]. Her short hair, androgynous looks, and prepubescent physique means that she looks boyish all the time. [[spoiler:She's only found out when she gets into a fight with a boy and his parents talk to her parents]].

[[folder: Literature]]
* In ''Literature/DragonBones'', Tisala of Callis has short hair, and a scarred face. She also doesn't look just like any dude, but extremely her ''father'', who, unfortunately, is not a Bishonen, but rather homely, in a way that's tolerable for a man, but defies the beauty ideals for women. She is also as tall as her father, who is considered a tall man. When Ward notices the latter, he pities her, as, he notes, it can't be easy for a woman to be taller than most men. (It takes him some time to notice as he's a GentleGiant, and Tisala's father is just about the height of his shoulders) [[AmazonChaser Doesn't stop Ward from having a crush on her.]]
* Jaime Lannister gives Brienne of Tarth a lot of shit for this in ''Literature/ASongOfIceAndFire'', specifically during the third book. At one point he even suggest she might be a female-to-male transsexual.
* ''Literature/AmericanGods'': in the narrative of the first people to come to North America (the ''first'' first people, not Columbus), one of the characters is a scout, technically female, who dresses like a man, has a male occupation, and a wife. She is accepted in this role and has a senior place in the tribe. Neil Gaiman [[ShownTheirWork shows his work]] with this one: historically, many Native American cultures had a practice by which a male or female person could fully embrace the duties, roles and appearance of the opposite and from that point on be treated as if they were born and lived the other gender.
* In ''Literature/AlexAndTheIronicGentleman'', it is repeatedly addressed how Alex, a girl, is mistaken for a boy because of her nickname and her bowl haircut.
* ''Literature/AutobiographyOfRed'': In Argentina, Geryon briefly mistakes a tuxedo-clad female tango singer for a man.
* When Jesse from ''Literature/BridgeToTerabithia'' first meets Leslie he is unable to tell her gender due to her short hair, unisex clothes, and GenderBlenderName. Her tomboyishness is a point of contention amongst the others in town because she doesn't fit what's expected for a girl. The 2007 film adaptation gave her a GirlinessUpgrade and made her wear bright clothes due to [[SocietyMarchesOn what's unusual changing]] since the books release. The 1985 made-for-tv film however kept Leslie's tomboyish look (albeit with slightly longer hair).
* In ''[[SesquipedalianSmith Merona Grant]] [[CharacterNameAndTheNounPhrase and the Lost Tomb of Golgotha]]'', Merona was mistaken for a man in the past.
-->'''Merona''': You punched me in the face the first time we met.
-->'''[[HuskyRusskie Sasha]]''': It was dark! And I thought you were man who owe me money.
* [[TomboyishName George]] from ''Literature/TheFamousFive'' is described as having [[BoyishShortHair very short hair]], almost as short as a boy's, that she cut because she hates long hair. She wears androgynous clothes and is prepubescent. She often gets mistaken for a boy as a result.
* The regiment in Discworld/MonstrousRegiment, who spend most of the book being girls pretending to be boys, at one point get back into dresses to pretend to be washerwomen and are mistaken for [[HarmlessLadyDisguise boys pretending to be girls]], because of their short hair and how much they've fallen into acting like men.

[[folder: Live-Action TV]]
* ''Series/GameOfThrones'':
** Ser Jaime Lannister (who is both shorter and considered to be way prettier than her) thinks Brienne of Tarth is far too masculine-looking for a woman. She's very tall, wears her hair short, dresses in men's clothing and armor and even bows rather than curtsies.
** Arya Stark in season 1 behaves like a tomboy and was frequently being mistaken as a boy due to her appearance, which annoyed her. After the death of [[spoiler: her father Ned Stark]], she had to crossdress as a boy to hide her identity from her enemies.
* ''Series/{{Monk}}'': Mr. Monk gets stuck in traffic, where Monk runs into a truck driver whom he initially assumes to be a male.
* UpToEleven in ''Series/SisterSister'' where Rhonda, formerly an AlphaBitch towards Tia and Tamera, re-appears in the season 4 episode "You Are So Beautiful" looking and sounding rather masculine.
* In ''Series/{{Miranda}}'', this happens to the title character on a fair few occasions. It even got to the point where in one episode she was ''mistaken for a man dressed as a woman''.
-->'''Delivery Man:''' Here you go, sir.
-->''(cue audience laughter and an annoyed AsideGlance from Miranda)''
* In ''Series/MarriedWithChildren'', Marcy was mistaken for a male several times due to her flat chest and short hair. This was mostly due to RuleOfFunny, since she wasn't especially unfeminine in appearance, and at least some of it was Al deliberately making fun of her.
* ''Series/{{Glee}}''[='=]s Season 2 (and on) football Coach Shannon Beiste, and a good amount of her characterization is owed to the real life experiences of Creator/DotMarieJones who portrays her.
* ''Series/{{Farscape}}'' has the Zenetans, a species with pretty much ''no'' sexual dimorphism. By the end of "The Flax," D'Argo has had it up to here with Staanz, a former space pirate and "garbologist" - only to find out that, not only is the male-looking Staanz a woman, but has really fallen for D'Argo. [[AllThereInTheManual Supplementary materials]] discuss how Zenetans rely on pheromones to tell which gender is which.
* Because she's wearing trousers and has her long hair tucked into a cap, Polly is mistaken for a young boy throughout the ''Series/DoctorWho'' serial "[[Recap/DoctorWhoS4E1TheSmugglers The Smugglers]]." While Ben pokes fun at her for it, she never really bothers to correct the mistake.
* In ''Series/TheKilling'', Holder initially mistakes [[ButchLesbian Bullet]] for a boy, thanks to her short hair and unkempt baggy clothes, as well as her overall masculine demeanour.
* Because of her androgynous appearance, Eleven in ''Series/StrangerThings'' is briefly mistaken for a boy in the first episode. This later leads to her case getting mixed up with Will's during Hopper's investigation.

[[folder: Multi-Media]]
* ''Toys/{{Bionicle}}'''s Hahli in her Toa Inika form wore a mask with [[GirlsWithMoustaches breathing-tube facial hair]], an enormous chin and a large, shiny bald head. It was given to her by Karzahni when he imprisoned her group in [[TheUnderworld his realm]] and took their own masks away. She didn't mind the look, but the fans, [[TheSmurfettePrinciple who viewed every female toy as a gift]], sure did.

[[folder: Theatre]]
* This trope is {{Older Than Steam}}. In Shakespeare's ''As You Like It'', the protagonist Rosalind dresses up as a man both out of curiosity and to escape persecution. [[spoiler:However, this comes back to bite her in the rear when she finds herself in love with Orlando, who is actually male.]]

[[folder: Video Games]]
* ''VideoGame/{{Touhou}}'': Wriggle Nightbug is female, [[ImprobablyFemaleCast like 99.99% of the cast]], despite her androgynous appearance (to the point of looking like a cute boy sometimes) [[{{Shipping}} and all the fanwork]] [[{{Rule 63}} to the contrary.]]
* ''Franchise/{{Pokemon}}'':
** The Machop and Timburr lines have a 25% chance of being female, despite their ''very'' manly appearances- the latter line's final evolution, Conkeldurr even sports a visible goatee!
** In ''[[VideoGame/PokemonRubyAndSapphire Omega Ruby and Alpha Sapphire]]'', Pokémon Coordinator Chaz has a Machoke named [[PunnyName Macherie]], who is female. You wouldn't be able to tell by just looking at her, but he refers to her by female pronouns, and insists on not teaching her Tough moves for contests (preferring Cuteness and Beauty), as she's "too cute" for them.
** Even better example: the very masculine looking [[BullyBulldog Granbull]] actually has a ''75%'' chance of being female. Its pre-evolution being [[PinkMeansFeminine pink]] may have something to do with it.
* [[spoiler:Elh Melizée]] from ''VideoGame/{{Solatorobo}}'', at least according to the characters in-game. This even allows Red to help her sneak into an all-boys magic school, insisting to the Ritual Master that she "couldn't be any more of a boy" - which, of course, annoys her immensely, since by that point she was quite fond of him. [[ViewerGenderConfusion Viewers tend to get confused]] when first introduced to her: since she wears a SkirtOverSlacks and has an [[{{Bifauxnen}} androgynous face]], most players assumed she was female, yet other characters insisted on referring to her as a boy and she [[{{Bokukko}} uses masculine pronouns in the Japanese version]], so players assumed they must've been mistaken. Then it's revealed that she ''is'' a girl after all; that SkirtOverSlacks look is apparently ''boys' clothing'' in the Shepherd Republic, which is why the other characters thought she was a boy. She never bothered to correct anyone because [[YouNeverAsked they just never asked which she was]].
* ''VideoGame/FinalFantasyVIII'': Adel. The only clue that she is a she is that she is called a sorceress.
* Anna Hottenmeyer from ''VideoGame/MrDriller''.
* Jherii Gallo, the ''VideoGame/DeadRising3'' Psychopath representing {{Pride}}, is an overzealous and arrogant bodybuilder with a deep voice and overmuscular physique. She tries to kill Nick when he accidentally [[BerserkButton keeps referring to her as a "sir"]].
* Mute from ''VideoGame/ArTonelicoQogaKnellOfArCiel''. She's a musclebound brute and virtually devoid of any femininity outside of her voice (Which is normal in the JP dub, but very masculine in the English dub) and SailorFuku. Aoto gives her the unflattering nickname of "Butch" in the English dub, but instead literally just refers to her as "man-woman" in the JP dub. [[spoiler: You later find out that she can transform into a typical loli when she feels like it, though.]]
* ''VideoGame/SenranKagura'':
** Miyabi is apparently this to the point where she constantly gets mistaken for a man and [[EvenTheGirlsWantHer her squealing fangirls as well as childhood friend Imu won't let her catch a break]]. Her story mode has her trying her very hardest to make herself more feminine. It ends up backfiring. Of course, this is very much an InformedAttribute since she lives in a WorldOfBuxom and her chest makes it obvious that she's a girl. The InformedAttribute does get lampshaded in one of the games where she asks Yozakura if there is anything feminine about her, and all what Yozakura can answer is her breasts.
** Kanzaki is another example. Similar to Miyabi, some of her cards imply that she tries to make herself more feminine as well.
* If you play ''VideoGame/PersonaQShadowOfTheLabyrinth'' as the protagonist of ''VideoGame/{{Persona 3}}'', Naoto is called "Intelligent looking boy" before her name is revealed. Some characters don't get that she's a girl until much later in the game.
* In ''VideoGame/IcewindDale'' the swarthy dwarven warrior in the [[YouAllMeetInAnInn opening inn]] is called Hildur. She gets faintly annoyed at being confused for a man, and having to explain to a non-dwarven party member that the [[GirlsWithMoustaches three foot beard is entirely normal]].
* ''VideoGame/PapersPlease'' has some female entrants whose heads look like men's heads. You have to strip search them to find whether or not the passport's stated biological sex is true or not in order to determine whether to let them in or to arrest them.
* In ''VideoGame/BlazBlueChronophantasma'', [[CampGay Amane]] [[DudeLooksLikeALady Nishiki]] calls [[ACupAngst Noel Vermillion]] the best cross-dresser he's ever seen. Why, she'd be perfect for playing the roles of women in his acting troupe! This enrages the normally ShrinkingViolet Noel enough to cause [[ArtificialHuman her Murakumo Unit]] [[EmotionlessGirl personality to take over]].
-->'''Noel:''' [[foldercontrol]]

[[folder: ...Target deemed hostile. Commencing termination. ]]

* Dorothy Albright from ''VideoGame/ArcanaHeart'' has a small case of this, in that just by appearance alone she might get confused by a young boy. It even comes to play in one of her victory animations where she passes off as a boy to fool the police (though she may also dress as a schoolgirl instead). Some fans reffer to her as "Reverse [[VideoGame/GuiltyGear Bridget]]" because of this.
* ''VideoGame/XenobladeChronicles2'': Between the standard military uniform and the fact that [[NeverBareheaded she never takes her hat off]], Morag looks very masculine, to the point that in a sidequest it's revealed that Tora didn't even know she was a woman.

[[folder:Visual Novels]]
* The PlayerCharacter of ''VisualNovel/LadykillerInABind'' is a young woman who is rather flat-chested and has androgynous facial features. This of course helps her a bit in pulling off the TwinSwitch her scheming brother has cajoled her into doing with him.
* Sakura Oogami from ''VisualNovel/DanganRonpa'' is an extreme example, to the point that she basically looks like a male bodybuilder wearing a SailorFuku. When Aoi mentions spending the night in Sakura's room, Kiyotaka is outraged at a girl sleeping with a "boy", before Sakura points out that she's a girl. Even in the Prologue when first meeting her, Makoto internally remarks that he almost asked her if she was a man.

[[folder: Web Animation]]
* ''WebAnimation/HomestarRunner'': "Some people wears glasses. Beth looks like a dude."
* Mai from ''WebAnimation/PorkchopNFlatscreen'' takes this UpToEleven: she can go around ''topless'' in the men's locker room and not draw attention.
* Parodied in ''WebVideo/DragonBallZAbridged''. Dodoria looks and sounds like a man, but is actually female, much to everyone's surprise.

[[folder: Webcomics]]
* In ''Webcomic/{{Hemispheres}}'', Mineshaft, the dwarf, looks like a [[OurDwarvesAreAllTheSame generic fantasy dwarf]] male... but, of course, it's eventually revealed that dwarves in this setting all look and sound male to humans, beards and all.[[note]]The situation for dwarves in the Literature/{{Discworld}} books is similar.[[/note]]
* Zii of ''Webcomic/MenageA3'' doesn't suffer from this regularly, as she generally presents a convincing and attractive feminine style, but she does have a very flat chest (and she is very mildly [[ACupAngst self-conscious about it]]). Hence, she is very annoyed in one early strip when Jung asks "Who's the skinny dude?" -- understandably, given that she's flashing everyone at the time...
* ''Webcomic/{{Same New Woman}}'' follows Marita, an ordinary librarian who wakes up one morning with a hyper-muscular body and extraordinary strength and is subsequently a source of gender confusion. She tries to go on with her old life but finds her world increasingly out of control.
* ''Webcomic/TheNoordegraafFiles'' has Violet. She doesn't talk, and is very, VERY flat - chested, so Theo initially mistakes her for a boy. The only possible way of noticing her true gender is that her arms are very feminine in appearance, and she has a ponytail.
* In ''Webcomic/BeyondBloom'', Tatsuma has this problem both in story and out of story. It is [[http://beyondbloom.webcomic.ws/comics/51/ stated ]] by the author that this was never intentional, but the gag was incorporated into the story because of so many readers and non-readers mistaking Tatsuma for a male.

[[folder: Western Animation]]
* ''WesternAnimation/AvatarTheLastAirbender'': Smellerbee is frequently mistaken for a boy, a misconception which she is somewhat sensitive about and quick to correct.
* ''WesternAnimation/BatmanTheBraveAndTheBold'' has Mrs. Manface, the wife of Babyface. She looks exactly like a dude, complete with five o'clock shadow.
* ''WesternAnimation/FamilyGuy'':
** The Griffins go into witness protection in the Deep South. Chris befriends a kid named Sam who looks and sounds like a guy -- until he kisses Chris and it's revealed shortly after that he is a she.
** Another episode, "Mind Over Murder", the homely, burly 'man' Peter punches turns out to be an incredibly ugly and ''pregnant'' woman, leading to her delivering her child ahead of schedule and his being placed under house arrest.
** A recurring joke is that Meg is mistaken for a boy (despite having a clearly female chest).
* Laney Penn from ''WesternAnimation/{{Grojband}}'', at least to the titular band.
* In the episode of ''WesternAnimation/HeyArnold'' where Helga gives herself a makeover a man mistakes her for a boy.
* Liz from ''WesternAnimation/TheMagicSchoolBus'', a rare case for a feminine animal.
* One episode of ''WesternAnimation/TheMarvelousMisadventuresOfFlapjack'' had Flapjack encounter a woman who looked like a hairy middle aged man with a long beard, he assumed at first that she was [[VocalDissonance a man with the voice of a little girl]].
* In ''WesternAnimation/MikeTysonMysteries'' several people have mistaken Yung Hee for a boy. Even a woman thought she was boy.
-->'''Yung''': [[LampshadeHanging Why does everyone think I'm a boy?]]\\
'''Marquess''': It's your haircut and I've told you that.
* Moose, the little sister of the title character in ''WesternAnimation/PepperAnn,'' not only looks like a boy but also [[{{Tomboy}} acts]] and [[TomboyishVoice sounds]] like one.
* ''WesternAnimation/TheSimpsons'':
** The episode "The Otto Show": Otto has to take a driving test, and Patty is his tester.
-->'''Patty:''' When you do good, I use the green pen. When you do bad, I use the red pen. Any questions?\\
'''Otto:''' Yeah, one. Have you always been a chick? I mean I don't want to offend you, but you were born a man, weren't you? You can tell me; I'm open-minded.\\
'''Patty:''' ''(Dropping the green pen)'' We won't be needing this.
** The episode "Selma's Choice" has a moment where the priest is eulogizing the deceased Aunt Gladys at her funeral:
-->'''Priest''': He was a caring man, he was a kind man. He gave to his community and asked little in return. He-
-->[A man whispers something in his ear.]
-->That's a ''woman''? Oh, dear God!
* ''WesternAnimation/ToddWorld'', a series targeted at preschool audiences. Todd's dog, Benny, meets a dog named Sam and they play together in the mud, so he never really gets a good look at her. When they meet later, Benny is shocked she's a girl -- "Sam" is short for Samantha. He thinks that he can't play with a girl, but learns differently. "Benny has a new friend now. Girls make great friends and so do boys!"
* In ''WesternAnimation/TotalDrama'', the JerkJock Lightning thinks that BrawnHilda Jo is a guy, leading to an amusing MistakenForGay situation when, in a life-threatening situation, Jo laments that she's going to die without ever having kissed a guy. Eventually he finds out that Jo is a girl and is completely shocked. It should be noted that Lightning's surprise wasn't justified, since Jo had already tried to tell him several times that she was a girl - he just didn't believe her until her elimination.
* ''WesternAnimation/TheAmazingWorldOfGumball'': Nothing about Coach Russo looks even remotely feminine: she has a huge, blocky {{Cephalothorax}} for a body and a masculine voice. It's only at the end of her debut episode that she is revealed as a woman (as well as being the mother of a feared bully).
--> '''Coach:''' And I'm not a woman... [[spoiler: who can say she's never lied on her résumé either]].
* ''WesternAnimation/TheAdventuresofJimmyNeutronBoyGenius'': Carl's mother is the ugliest female on the show--with a hoarse ''masculine'' voice. Helps that like Carl and Mr. Weezer, she's voiced by Creator/RobPaulsen.

[[folder: Real Life ]]
* Mary Frith, a.k.a. Mad Moll Cutpurse, was a well-known pickpocket and pimp in the English Renaissance who dressed as a man, dueled other men, smoked a pipe, and swore. She was married to a man eventually, but it was largely believed to be a domestic farce. Thomas Middleton's play ''The Roaring Girl'' was based on her.
* Music/CarlySimon has often been compared with Music/MickJagger's looks.
* [[Music/ThePixies Kim]] and [[Music/TheBreeders Kelley Deal]]. Wearing lumberjack shirts and smoking like chimneys hasn't helped
* [[http://en.wikipedia.org/wiki/Anne_Bonney Anne Bonney]] and [[http://en.wikipedia.org/wiki/Mary_Read Mary Read]], female pirates in the early 1700's. Sources differ on if or how long they disguised themselves as men, though.
* Music/PattiSmith
* UsefulNotes/CalamityJane, who dressed up as a man and swore and drank like them too.
* Jeanne d'Arc (better known as UsefulNotes/JoanOfArc in English-speaking countries) cut her hair and dressed as a man during the Hundred Years War. Actually, when she was on trial by the English for heresy, this was one of the only things they could convict her of, and thus was one of the sole reasons she was burned at the stake.
* In Britain, during times when hairstyles either favored shorter hair for women or longer hair for men, it could be hard to tell the difference between a man and a woman who just wore trousers (or for pre-adolescents, a girl could easily be mistaken for a boy who hadn't hit puberty).
* [[https://en.wikipedia.org/wiki/Spotted_hyena Spotted Hyena]] females look almost exactly like the males, right down to having an enlarged clitoris that's essentially a pseudopenis, and fused labia that look like a scrotum (until after giving birth for the first time, when they are torn apart).
* Dairy cows often are understandably mistaken for being male. It isn't a well-known fact that cows and bulls are the same species.
* More a case of Lady ''acts'' Like a Dude, but [[https://en.wikipedia.org/wiki/European_robin European Robins]] (not to be confused with American robins), aside from the sexes looking the same, are exceptional among birds for their females being just as territorial, and just as aggressive (this bird species is one of the few who will fight each other to the death) as the males, and the females even ''sing'' to mark their territory, something only males do in other bird species.
* When Creator/FoxNewsChannel posted an editorial in favor of traditional gender roles, [[http://feministing.com/2013/02/09/woman-from-fox-news-accidental-same-sex-wedding-picture-speaks-out/ they illustrated it with a photo of a lesbian wedding]], which they had ironically mistaken for a depiction of a heterosexual couple due to the masculine appearance and attire of Stephanie Figarelle. Once this was pointed out, the photo was replaced with a more generic one.
* [[http://en.wikipedia.org/wiki/Catalina_de_Erauso Catalina de Erauso]], a straight up ButchLesbian who escaped the convent she had been secluded in at age ''four'' by joining the ''army''. She had several female lovers who never realized she wasn't a man and served in the same company as her brother, who didn't recognize her.
* Talk show host [[http://sandrarose.com/2014/07/wendy-williams-gets-why-people-say-she-was-born-a-man/ Wendy Williams]] has often been mistaken for a crossdressing man or {{transgender}} woman because of her height, relatively manly features, and over the top wigs and make up. However, despite all this, she is in fact a cis woman, and has a child and had many miscarriages.
* Actress/voice actress Charlyne Yi, [[https://www.youtube.com/watch?v=WFfKvP4vSL0 according to Sri Lanka]].