%% There are examples filed under "Film - Live Action" at present that do not have a title of the work provided to properly identify the source of the described trope occurrence. If anyone can provide a title for the movie based on the present description, please add it.
%% Image selected per Image Pickin' thread: http://tvtropes.org/pmwiki/posts.php?discussion=1479602113000936400
%% Please do not replace or remove without starting a new thread.
[[quoteright:350:[[ComicBook/{{Hellboy}} http://static.tvtropes.org/pmwiki/pub/images/anal_probing.jpg]]]]
[[caption-width-right:350:Greetings, Hellboy, we have traveled millions of light years to explore new planets...and now, we seek to explore {{Uranus|IsShowing}}!]]

->'''Alien 1:''' Couldn't we, at least, abduct their political and religious leaders, instead of just any idiot in a pickup truck?\\
'''Alien 2:''' I'm sure the Great Leader has his reasons.\\
'''Alien 1:''' Well, ''I'm'' sure the Great Leader is just some sort of twisted ''ASS FREAK!''
-->-- ''Series/TheKidsInTheHall''

More often than not, humans taken on board a spaceship against their will are subjected to grueling tests and experiments by their [[LittleGreenMen Strange]] [[TheGreys Captors]] for intended purposes ranging from studying foreign biology, researching the prospects of [[HalfHumanHybrid interbreeding]], and monitoring for long periods of time via tracking chip implants.

Fortunately for the abductors, all three of those things can be achieved through a single method which involves [[AssShove inserting vaguely described alien technological instruments into the rectal cavity of the victim]]. If the audience learns ''anything'' about the probing tech itself, it's usually never much more than just what it looks like, which is typically either an insanely large torture device with pincers and needles and spinning blades mounted on a rotating head or a dildo.

Naturally, the trope can be used to great effect to either showcase the sheer horror of or, more frequently, use VulgarHumor to explore the total absurdity of the nature of {{Alien Abduction}}s; it can also be a novel way of fitting jokes about [[HoYay gay sex]] (abductees depicted as being subjected to this are much more frequently male) and [[BlackComedyRape rape]] into a story or work.

Anal Probing is a DeadUnicornTrope. This trope is rarely, if ever, taken seriously in fiction, and even when it's supposed to be seen as something truly horrible and undesirable, there's usually enough room to interpret the trope's use as ComedicSociopathy. RealLife UFO abduction communities, which would seem like the most likely place to hear straight-forward examples, don't even discuss probing as something that exclusively involves the rectal cavity (if they do at all).

The ScienceFiction equivalent to a cavity search.

Be on the lookout as well for {{pun}}s about space programs launching probes to [[UranusIsShowing Uranus]].

See Also: AssShove, VulgarHumor, DoubleStandardRapeMaleOnMale; HalfHumanHybrid and MarsNeedsWomen which are both popular motivations for one kind of alien probing or another. This trope is practically synonymous with TheGreys and is often evident whenever AliensAreBastards, especially if the probing doesn't appear to have any real significance apart from torturing the abductee.



* In one radio spot for Dos Equis beer, the narrator describing Advertising/TheMostInterestingManInTheWorld says about him, "Alien abductors ask him to probe ''them''."

* There's an old joke where a group of aliens is having a conference about Earth. The keynote speaker gets up and informs the gathered scientists that, "We have now been performing our rectal probe study on the inhabitants of Sol 3 for 60 of their years. So far, the only information we have reliably obtained is that one in six likes it."
* One of the ''Blog/ThingsMrWelchIsNoLongerAllowedToDoInAnRPG'' involves the ''Franchise/StarTrek'' Federation finding those "little grey butt-probe aliens to return the favor".

[[folder:Comic Books]]
* Pierre from the ''ComicBook/TwoThousandAD'' strip ''Bec & Kawl'' was abducted in the prologue to "Attack Of The Cones." Explaining his experience, "Whatever their ''[[GratuitousFrench desseines impenetrable]]'', the temple of my body was violated," showing one panel with two Greys producing increasingly larger probing apparatuses with diagrams instructing to insert them in Pierre's anus.
* [[LampshadeHanging Lampshaded]] in ''Maxwell Strangewell'', where the Moon Man complains to a Martian, "All you Martians care about is probing people up the bum and [[AliensStealCattle slaughtering cattle]]!" The Martian's response: "Hey, it's a living."
* The Gagwallers from the "Spacehack" strip in ''ComicStrip/KnightsOfTheDinnerTable'':
-->'''Captain Queaze:''' After all these experiments... over all these centuries, what have you learned?\\
'''Gagwaller 1:''' We have learned... that most of... them don't like the... probing.\\
'''Gagwaller 2:''' Well... ''most''... of them.
* In ''ComicBook/{{Squee}}'', when the wheelchair-bound aliens try to abduct Squee for a second time, one of the two aliens goes to his parents' room and subjects Mr. Casil to this.
* Almost happens to ComicBook/{{Hellboy}} the time he is abducted by aliens. He decides he doesn't like the idea and proceeds to kick all their asses.
* The trope is played horrifyingly straight in ''ComicBook/SaucerCountry''. As part of the abduction of the lead and her ex-husband, both are probed by aliens. Their reactions make it very clear that there was ''nothing'' funny about it.
* Aliens do this to Cherry during ''Series/TheXFiles'' parody in ''Cherry Comics''. Cherry, naturally, [[AnythingThatMoves enthusiastically enjoys it]].

[[folder:Comic Strips]]
* ''ComicStrip/KnightsOfTheDinnerTable'': The "Black Hands 2011 Special" ends with the Black Hands characters being anally probed by aliens after Weird Pete decides to combine his ''Cattlepunk'' campaign with ''Scream of Kachoolu''.

[[folder:Fan Works]]
* The following exchange takes place between Paul and the alien Varx in the Prologue of ''Fanfic/WithStringsAttached'' when Varx is trying to convince Paul to go on a "great adventure":
-->'''Paul:''' Is this one of those alien abductions where you're gonna probe me, then?\\
'''Varx:''' ''[snorting]'' Gods, you Earthians are masochists. Believe me, if we really wanted that info, which we don't, we could do a deep scan right from our own universe and you'd never know it happened.
* In ''Plan 7 of 9 from Outer Space'' by Odon, the Martian [=TuMok=] explains this was due to a biological misunderstanding. It was supposed to be a MindProbe but "we assumed from human behavior [[HumansAreMorons that your brains were located in your behinds]]."

[[folder:Films -- Animation]]
* In ''WesternAnimation/JourneyToSaturn'' the Danish astronauts (all of them male) have been captured by the Saturnians, who subjects them to this procedure. They all scream in pain as it happens, and anguish over their lost dignity and manhood afterwards, except for [[AnythingThatMoves Ole]] who genuinely enjoys the whole experience and acts as if he just got a massive itch scratched.
* ''WesternAnimation/Planet51'': Skiff gives Lem a cork to put it in certain place on his body in order to defend against the "alien's favorite form of research, the probe".
* Hilariously subverted in ''WesternAnimation/TitanAE'' when Cale is in the sick bay and he thinks Akima is gonna do this to him.
--> '''Akima:''' Hand me the probe.
--> '''Cale:''' The probe? Uh, where does the probe go? Ya'know, I'm really feeling much better. ''[Akima jams the probe into a wound on Cale's thigh]''

[[folder:Films -- Live-Action]]
* In the {{Mockumentary}} ''Film/WaitingForGuffman'', a supposed visit by a UFO is a significant part of the history of Blaine, Missouri. One abductee shares his story, in which he details being probed for "three to four hours" by "five or six" aliens--not all at once but individually. Every Sunday around the time of his abduction, the abductee finds that he has "no feeling in his buttocks."
* Happened in the beginning of the AlienInvasion [[SlidingScaleOfComedyAndHorror Horror Comedy]] ''Evil Aliens''. Including a close-up of the probe getting inserted into the anus.
* In ''Film/IndependenceDay'', Russel Casse attests to having been abducted by aliens. Those who don't believe him crack jokes and ask if he was ever sexually abused on the flying saucer, suggesting this trope.
* In ''Film/MenInBlackII'', Sci-Fi nerd Newton, upon finding out what Jay and Kay actually do, raises the question, "What's up with Anal Probing?"
-->'''Newton:''' I mean, do they really travel billions of lightyears just to...\\
'''Agent J:''' Boy, move.
* There's a bit in ''Film/EightLeggedFreaks'' where Harlan goes on a tirade about aliens and this very subject. "...I mean, what do they expect to find there? It's just wrong!"
* In ''Film/{{Paul}}'', the titular alien appears to have assimilated human culture after 60 years on the planet, and is your average slacker-nerd. However, Clive isn't so sure and asks Graeme, "What if we wake up and find him inserting a probe into our anus?" Paul is annoyed by the suggestion. Wondering why people assume aliens do that, he asks, "Am I harvesting farts? What can I learn from an ass?!" Paul does, however, threaten a pair of homophobic hillbillies with probing. On Twitter, Simon Pegg mentioned that they did consider doing a joke about anal probing and repressed memories (of sexual abuse), but they ditched it because they couldn't find a punchline and felt that it might actually be true and [[DudeNotFunny not funny]].
* In ''Recap/MysteryScienceTheater3000TheMovie'', Servo riffs on the practice a couple times.
-->''[Dr. Cal Meachem get pulled into a flying saucer by a beam of light.]''\\
'''Tom Servo:''' ''(as Dr. Meachem)'' Oh great. And I just know they're gonna probe my anus.
* Creator/ChristopherWalken's character reports this in the film adaptation of ''Film/{{Communion}}'' (see Literature).
* Anal Probing is {{Invoked|Trope}} by the {{Space Marine}}s on occasion in ''Recon 2020: The Carpini Massacre''. Anyone thought lost or gone missing is assumed to have been "either anal probed to death or turned into a cyborg."
* The 2014 sci-fi horror film ''Film/{{Extraterrestrial}}'' suggests rectal probing. The film's social media promo campaign even uses the hashtag "[=#GetProbed=]".
* ''Film/JusticeLeague''. A woman is shown on the news insisting that her husband has been kidnapped by aliens (which is actually true) and goes off on a [[SoundEffectBleep bleeped out]] rant regarding what she'll do to those probing aliens.
%% * A short film parody had ''Series/TheXFiles'' InTheStyleOf the silent film era ''Keystone Cops''. Our comedic heroes come across a 'moon man' in a park about to abuse a hapless human with his pants down. He even has [[MyCard a business card]] advertising his services: ''Rustics amazed, yokels abducted, derrieres probed''.

* In the ''Literature/{{Nightside}}'' series a Grey is seen lying in the gutter with a "Will probe for food" sign.
* ''Literature/{{Communion}}'' by WhitleyStrieber, the allegedly true story of the author's experience of a UFO abduction helped establish this trope and has inspired just about every joke about rectal probes, much to Strieber's dismay, ever since. It's probably the closest thing to a straight example of this trope one can find. Even the FilmOfTheBook, starring Creator/ChristopherWalken as Strieber, made light of the probing with Walken's character trying to reason, "Can we talk this over? It looks like you're going to sing 'White Christmas'..."
* Christopher Buckley's novel ''Literature/LittleGreenMen'' reveals alien abductions to be the work of a GovernmentConspiracy to manufacture evidence of alien activity. Rectal probing wasn't actually their doing but rather something that the abductees seemed to have ''demanded''.
* "Angel Down, Sussex", a ''Literature/DiogenesClub'' story by Creator/KimNewman, features alien visitors whose appearance and actions vary depending on the expectations of the people they encounter. That one of the characters gets his rectal cavity probed really says more about him than about the aliens.
* ''Literature/EarthTheBook'' mentions Anal Probing in the Foreword as one thing humans who believe aliens have already visited Earth think extra-terrestrials came here to perform. It then refers to the anus as "the orifice least likely to yield useful pedagogical results." Mykonos, Greece is described as the one place that would have been the most receptive to Anal Probing.
* A pastime of the titular ''Literature/TheAssGoblinsOfAuschwitz''. No reason is given why they do it; it's just apparently something that they do.
* Robert J. Sawyer’s ''Calculating God'' has a alien invoke this during first contact:
-->'''Hollus''': Of course, if you want, I could give you an anal probe... Sorry—just kidding. (...) Honestly, I will not hurt you—or your [[AliensStealCattle cattle]].
* Creator/PiersAnthony's ''Firefly'' involves an alien who practices OrificeInvasion on his victims. Once prospective victim muses during the attack that he's being "cornholed" by an alien and, worse, he was enjoying it.
* In the erotic comedy ''Rock 'n' Roll Babes From Outer Space'' by Linda Jaivin, the Nufonians put a TrackingDevice in Jake's behind that causes him to fart whenever it's activated.

[[folder:Live-Action TV]]
* ''Series/ThirdRockFromTheSun'':
** One episode began with the main characters watching an episode of ''Series/TheXFiles'' on television, groaning over how laughably inaccurate the show is. One scene, described by Tom as an alien inserting a probe through someone's ear, is met with the derisive comment from Dick, "''Everyone'' knows the proper place to insert an alien probe is the butt!"
** In another episode, Dick is captured by an insane alien hunter who accuses aliens like him of "probing the butt of the poor American farmer". Dick initially denies this, but after being pressed, he mentions a farmer near Akron "...but he was ASKING FOR IT!"
** Dr. Albright's brother Roy claims that this happened to him.
* ''Series/{{Supernatural}}'':
** In the episode "Tall Tales" in which the Trickster forges LOTS of strange happenings in a college town, one of them involves a guy who's been abducted by "aliens." The victim's experience involved [[BlackComedyRape being repeatedly probed]] (a total of eight times according to the dialogue). This ordeal apparently paled in comparison to being forced to slow-dance with one of the aliens to "Lady in Red."
** In the sixth season episode "Clap Your Hands If You Believe", when Dean informs Sam that he is fleeing from a pursuing UFO, Sam pronounces this a Close Encounter of the Third Kind and [[ComedicSociopathy suggests that Dean keep running as he has "heard the Fourth Kind is a butt thing".]] Dean reminds him that he's [[CameBackWrong supposed to be feigning empathy]].
* An episode of ''Series/{{House}}'' featured a young boy who believed he'd been abducted by aliens, and he had the bleeding anus to prove it. This obviously led the team to believe he'd been sexually abused, which (predictably for House) turned out not to be true.
* The ''Series/TheKidsInTheHall'' have a [[https://www.youtube.com/watch?v=6tZar4wRP40 sketch about aliens]] (quoted at the top of this page) that [[LampshadeHanging hangs a lampshade]] on this trope. After doing this for over fifty years, one alien argues that they haven't learned anything from probing, except that one out of every ten "doesn't seem to mind". He wonders if his Great Leader is just some kind of weirdo with a disturbing fetish.
* Referenced and [[PlayingWithATrope Played With]] often on ''Series/TheXFiles''. In "Jose Chung's 'From Outer Space,'" for instance, it's AgentScully's opinion that a young couple whom [[AgentMulder Mulder]] believes were abducted by aliens were only engaging in sexual activity before they're old enough to handle it. Mulder, not seeing how this could discredit the girl's interpretation of events as revealed under hypnosis (which resembles the typical AlienAbduction story), asks his partner, "So what if they had sex?" to which Scully responds, "So we know it wasn't an alien that probed her."
* When Alien Abductions were covered on ''Series/PennAndTellerBullshit'', they covered a sex toy with silver spray paint to show people who claim to have been abducted. Upon seeing the sex toy, many claimed they were probed with a similar device. They made a game out of this where they paused the tape before one female abductee disclosed where in her body the aliens had inserted a probe and encouraged viewers at home to ''guess'' what part of the body it was. The correct answer was [[spoiler: [[NoseShove the abductee's nose.]]]]
* {{Discussed|Trope}} in the seventh episode of Volume One on ''Series/{{Heroes}}'', when Lyle discovers Claire's [[HealingFactor ability to heal herself]] after stumbling upon a tape that she and her friend Zach made to demonstrate her ability, this forces Claire and Zach to chase after him to retrieve the tape. A freaked out Lyle questions if they're both actually aliens to which Zach responds, [[DeadpanSnarker "Yeah, and we're gonna anal probe you."]]
* A segment on ''Series/TheColbertReport'' about an "Alien Hunter," Derrel Simms, sees Mr. Simms recount a story about him being probed by aliens in his youth. After describing the alien probe in very phallic terms, Colbert interrupts him in a voice over just before Simms was about to say where the probe was "jammed very painfully," exclaiming, "Okay, that's enough; I think we ''all'' know where the probe goes." Simms then reveals that it went into [[spoiler: [[SubvertedTrope his nasal passage.]]]]
* Invoked in ''Series/TheFinder'', episode "Little Green Men". The lead character disappears for a couple of days while researching alleged alien activities, and one of the first questions when the Finder turns up back at his home is "Was there anal probing?"
* Referenced a couple of times in the ''{{Series/Psych}}'' episode "Not Even Close...Encounters". At one point, as they are fleeing in terror from a UFO, Shawn yells to Gus," I don't wanna get probed, Gus!"

* Anal Probes are mentioned in the Newton Faulkner song "U.F.O.".
* Music/TheGreatLukeSki mentions anal probes in a filk song about ''Film/MenInBlack''.
* Music/WeirdAlYankovic refers to alien butt probing in the song "Foil".
--> ''Wear a hat that's foil-lined,\\
in case an alien's inclined,\\
To probe you butt or read you mind.''

* The quasi-improv theater group Sound and Fury used this trope in "Cyranose," while Cyranose is trying to distract De Guiche by pretending to be a lunatic:
-->'''Cyranose:''' They probed me--\\
'''De Guiche:''' Eeew!\\
'''Cyranose:''' For information.\\
'''De Guiche:''' Oh.\\
'''Cyranose:''' Anally. A few times. ''[beat]'' It's an acquired taste.
* The second act of ''In the Next Room, or the Vibrator Play'' introduces the Chatanooga anal vibrator.

* This is Cartman's mode in Sega's ''Pinball/SouthPark'' pinball.

[[folder:Video Games]]
* ''VideoGame/Fallout3'' downloadable quest package "Mothership Zeta" sees [[PlayerCharacter the Lone Wanderer]] abducted on board a spaceship and probed.
* In the sequel games in ''Franchise/TheSims'' series--''VideoGame/TheSims2'', ''VideoGame/TheSims3'', ''VideoGame/TheSims4''--the (actually light green) Greys have pollination technicians, who abduct, probe, and impregnate Sim men.
* ''VideoGame/DestroyAllHumans''. In the first game, the anal probe is a ChargedAttack that can make the victim's head explode. It returns in the second game without the need for charging, though it now requires ammo. [[VideoGameCrueltyPotential Alternatively]], it could be employed in a less-than-lethal manner without charging it, which would simply cause the humans to [[PottyEmergency run about in a panic]] as [[BringMyBrownPants their bowels are violently and involuntarily evacuated]].
* ''VideoGame/TheSimpsonsGame'' features this exchange between two of Kang and Kodos's {{mook}}s during the AlienInvasion level:
-->'''Alien 1:''' This human has tiny pieces of paper which gives him big, big savings. We must study him!\\
'''Alien 2:''' Study him with Anal Probing?\\
'''Alien 1:''' Of course with Anal Probing! What else would we do?! Talk to him?!
* ''Franchise/MassEffect'':
** Referenced in ''VideoGame/MassEffect2'' - you can probe [[UranusIsShowing the planet Uranus]], and [[http://www.youtube.com/watch?v=d-CDNLYZ0zA EDI is not amused]] when you do. The planet is even considered 'depleted' (...of minerals, although there are a few small pockets of resources to mine) on the scanner to logically discourage you to scan it aside from [[PlayedForLaughs Just For Laughs]].
--->'''EDI:''' ''Really'', Commander?\\
'''EDI:''' Probing Uranus.
** In ''VideoGame/MassEffect3'', Joker says he suspects Mordin planted a probe in his elbow. Shepard tells him to be glad it's just his elbow that got probed.
* In ''VideoGame/DisgaeaHourOfDarkness'' human [[CovertPervert Jennifer]] asks [[Characters/DisgaeaHourOfDarkness Laharl and co.]] if the demons (who basically are aliens in the ''Franchise/{{Disgaea}}'' universe) anal probed them. It's a {{Subversion}} because Laharl and Etna are young demons and no demon in the series are interested in probing either them or Jennifer.
* In ''VideoGame/SuperScribblenauts'', creating an alien and a probe will result in the alien making a '?' bubble and going to pick up the probe. The probe doesn't do anything.
* ''VideoGame/SaintsRowIV'' features an alien anal probe as a weapon to be wielded by the player. The player can approach a target from behind and thrust the weapon between the victim's legs, before pulling a trigger which launches the victim into the air.
* In ''VideoGame/SouthParkTheStickOfTruth'', the New Kid gets abducted on the first night in South Park and winds up getting probed, but his ass muscles are so powerful that he winds up breaking all the probes they pull out. The last one sucks itself further up his ass and then becomes a satellite dish that you can use as a short-ranged teleporter to alien receivers all over the town.

[[folder:Myths & Religion]]
* This sort of story is not limited to aliens. Some residents of the Zanzibar Islands believe in [[http://istina.rin.ru/eng/ufo/text/294.html a sort of cryptid called the Popobawa]], a foul-smelling bat-like demon who sodomizes men in their sleep, and unlike most demonic attackers, demands he spread the word, or else it will be back. One unfortunate villager (who was known to be mentally ill) claimed to ''be'' this creature, and was hacked to death by someone who believed him.

[[folder:Web Comics]]
* In ''Webcomic/RasputinBarxotka'', during the Moscow alien invasion.
* ''Webcomic/SluggyFreelance'' has TheGreys in its "Oceans Unmoving" arc, apparently having arrived in Timeless Space from the game ''{{VideoGame/XCOM}}''. They make continual references to probing; later they explain that they have been genetically engineered to have an instinctual drive to shove things into holes.
* ''Webcomic/ThogInfinitron''. The title character is trampled by a herd of mammoths when an alien research robot attempts to administer an anal probe to an unsuspecting mammoth.
* ''Webcomic/{{Chainsawsuit}}'' [[http://chainsawsuit.com/2009/03/02/strip-172/ puts this into context]]. With one phrase...
--> '''Grey Alien:''' Our kind has traveled 10,000,000 light years to unlock the secrets... in your butt.
* A truly horrible probing is [[http://www.channelate.com/2009/09/14/can-i-abduct-you-pt2/ implied]] at the end of a two-part comic in ''Webcomic/ChannelAte''.
* Invoked in a ''Webcomic/{{Wulffmorgenthaler}}'' strip by a very lonely farmer who ''wants'' to be anal probed and [[http://wulffmorgenthaler.com/strip/2010/01/20/ jumps at the chance to be abducted.]]
* ''Webcomic/SchlockMercenary'' used it [[http://www.schlockmercenary.com/2005-11-27 as a metaphor.]]
* Unity of ''Webcomic/SkinHorse'' once [[InvertedTrope probed an alien]].
-->"If it [[LittleGreenMen looks like Kermit]], I'm gonna look for Brian Henson!"
* Done in ''Webcomic/TheKamics'' with the punchline being that the probe was intended to be inserted into the abductee's throat, [[http://www.theduckwebcomics.com/The_KAMics/4770715/ but the aliens mistook the wrong orifice for that.]]

[[folder:Web Original]]
* The 2006 Halloween event at ''Website/GaiaOnline'' centered around the themes of AlienAbduction and AliensStealCattle with probing devices becoming available items with which to decorate user avatars.
* The ''[[http://www.8legged.com/ Deep Fried Live]]'' episode [[http://www.8legged.com/DeepFriedLive/DFL01_07.html "Fleeb Cooks A Cow"]] features a [[LittleGreenMen Little Green Man]] who lists his occupation as "probe technician" who seeks Chef Tako's assistance in learning [[AliensStealCattle how to cook a cow]], although he seems much more interested in probes. The alien even reveals his probing device, which resembles a human forearm and hand with the index finger fully extended.
* WebVideo/YuGiOhTheAbridgedSeries has this bit of dialogue between [[CloudCuckoolander Tristan]] and [[AnythingThatMoves Duke]]:
-->'''Tristan:''' Dude, do you ''want'' the aliens to probe your anus?\\
'''Duke:''' ...Maybe.
* Website/DeviantArt has a smiley for this.
* Creator/FrancisEDec warned in his rants that Gangster Computer God's servants do this to people at night, when we are all "[[MindControl Frankenstein zombies]]".

[[folder:Western Animation]]
* ''WesternAnimation/SouthPark'':
** Eric Cartman was probed by TheGreys in the {{Pilot}} episode, appropriately titled [[ExactlyWhatItSaysOnTheTin "Cartman Gets An Anal Probe"]].
** It was later revealed in "Earth is Canceled" that Earth is actually an alien reality show, and that the device up Cartman's ass (and presumably others) is tasked with the TV transmission.
* ''WesternAnimation/TheSimpsons'':
** The HalloweenEpisode short "Citizen Kang" from "Treehouse Of Horror VII" saw Homer abducted by Kang and Kodos. Homer's reaction to this is to say, "I suppose you want to probe me. Well, might as well get it over with..." immediately dropping his pants and mooning his alien captors. Kang and Kodos respond to this with horror, begging, "Stop! We have reached the limits of what rectal probing can teach us!"
** In another episode, whilst searching an Air Force base for Sideshow Bob, an airman opens the door to Hangar 18 before quickly closing it again after seeing a Grey with a glowstick. The airman proclaims, "Watch out! He's got his probe!"
** When Homer and Flanders marry floozies in Vegas while drunk, Homer decides they should tell their wives they were abducted by aliens, who "gang-probed" Ned.
--->'''Ned:''' Do we really have to tell them I was "gang probed"?\\
'''Homer:''' Would you rather tell them the ''truth?''\\
'''Ned:''' ''[sigh]'' What did the aliens look like?\\
'''Homer:''' Well, ''I'' only saw them from the back 'cause they were so busy gang probing you!
** In "The Springfield Files", Homer tells a story how he met an alien on Friday night. He says they sat him on a cold, metal table and prodded him with humiliating probes, only to realise he was confusing his encounter with his physical at the hospital.
* ''WesternAnimation/HarveyBirdmanAttorneyAtLaw''. Phil Ken Sebben mistakenly believes WesternAnimation/TheJetsons are Greys--not time travelers--and that they're out to give him an anal probe. "Ha HA! [[Film/CloseEncountersOfTheThirdKind Third Kind]]."
* ''WesternAnimation/LifesAZoo''. When Ray believes he's been abducted by aliens he thought it was awesome, until he figured that: Alien Abduction = Anal Probe.
* In ''WesternAnimation/{{Futurama}}'', Fry is abducted by a flying saucer whose vanity plate reads "PROBE #1."
* ''WesternAnimation/RobotChicken'':
** Inverted. A group of flannel-wearing, toothless rednecks in a pickup truck [[https://www.youtube.com/watch?v=z-g4FWpxjYI abduct an alien on his home planet.]] Then later, in a simultaneous parody of this trope and ''Film/{{Deliverance}}'', the hicks gleefully surround the alien, bent over and tied to a tree stump, and [[https://www.youtube.com/watch?v=r3mE8RvQVOU bluntly announce]] how they're going to perform "scientific experiments deep inside {{Uranus|IsShowing}}".
--->"Now SQUEAL like a pig!"
** Another episode had the aliens [[https://www.youtube.com/watch?v=1-0-dQHAM2o wondering]] how the story of them doing that got started.
--->"This is gonna be a PR nightmare, you know that?"
* ''WesternAnimation/AquaTeenHungerForce'' indirectly offered an interesting theory as to why aliens anally probe. While dealing with a PuppeteerParasite alien that was controlling Shake, Frylock said he probably recycles his waste (he didn't). Therefore, one reason for anal probing may be that aliens lack anuses and are curious as to their purpose.
* {{Subverted|Trope}} in ''WesternAnimation/TheTick'': when the Tick is abducted, he is enthusiastic about being probed to expand interstellar knowledge, but it turns out the aliens actually want him to help defeat their enemies. The thought of probing him never crossed their minds. Eventually, they concede to probe him... by tapping him on the head with a stick. [[GettingCrapPastTheRadar Tick seems disappointed.]]
* In a ''WesternAnimation/FamilyGuy'' CutawayGag, Quagmire has been abducted by aliens and is inquiring about anal probing. They reply that they don't do it anymore. [[{{Beat}} After a short pause]], he asks if they still have the device.
* In ''WesternAnimation/AmericanDad'', Roger's alien race can acquire a person's memories through probing.
* In ''WesternAnimation/TheVentureBros'' episode "Tears of a Sea Cow", Hank and Dermott mistake the Monarch's cocoon for a UFO and his henchmen for aliens. Dermott shoves a lighter up his butt so that if the aliens try to probe him, they'll get a "face full of fire."
* In one episode of ''WesternAnimation/CodeMonkeys'' Todd and Dean get abducted by aliens. Probes are inserted and a meter shows how far up their butts the probes are.
* On ''WesternAnimation/TripTank'', [[https://www.youtube.com/watch?v=I9W816W40nE a sketch]] reveals that aliens' fascination with probing came when they abducted a drug trafficker and tried to implant a probing device inside his anus, only to find that there was already something up there--a bag of cocaine that he was smuggling. Every subsequent probing has been part of a quest for more cocaine.
->'''[[JustForFun/StatlerAndWaldorf Waldorf]]:''' You know, this isn't a bad trope these kids have here.\\
'''[[Series/TheMuppetShow Statler]]:''' Well, I think it's a real pain in the ass!\\
'''[[ThoseTwoGuys Both]]:''' Doh-ho-ho-ho-hoh!