* In ''WebVideo/EchoChamber'', [[PsychoExGirlfriend Shannon's]] entrances are marked by PsychoStrings.
* ''[[EvangelionReDeath It's Gendo]]!''
* Whenever Sting showed up in ''{{Dune}}'' wearing a speedo, WebVideo/TheNostalgiaChick would play "Don't Stand So Close To Me".
* Any appearance by Tex in ''[[Machinima/RedVsBlue Red Vs. Blue]]'' is usually accompanied by the opening bars of [[http://www.youtube.com/watch?v=xIg1vZPKFyU her theme.]]
** With the exception of Season 8, though her new theme, [[http://www.youtube.com/watch?v=W_PB0XguvE0 Agent Tex]] contains a nod to her original leitmotif.
** [[BigBad The Meta]] has one in the ''Recollections'' seasons: [[http://www.youtube.com/watch?v=tw2VDWB-u2Y "(When) Your Middle Name Is Danger"]].
** Carolina doesn't officially have her own, but when she's fighting, expect some variant of [[http://www.youtube.com/watch?v=5Lhz7V1mQQY Extraction (starting at about 2:08)]]. The tune has been played differently numerous times depending on the setting, including [[http://www.youtube.com/watch?v=fsMWvraCUVI with guitars near the end of Spiral]], [[http://www.youtube.com/watch?v=mk8USjPqiFU twice in Mental Meta Metal]], and [[http://www.youtube.com/watch?v=ZDz0woffIsI with a particularly heart-wrenching piano in Ballet Breakup]].
* In ''[[WebVideo/YuGiOhTheAbridgedSeries YuGiOh Abridged]]'', the opening for ''[[JustinTimberlake Sexy Back]]'' loops whenever Duke Devlin speaks. Also, the "Double Fine" riff of Judas Priest's ''Hellion'' plays at the beginning of any of [[ElmuhFuddSyndwome Dartz's]] scenes.
* The LetsPlay/{{Yogscast}} has "I'm a dwarf and I'm digging a hole! DIGGY DIGGY HOLE!" and variations of such. It comes from ''ShadowOfIsraphel'', when LetsPlay/SimonLane is digging for [[spoiler:K. Peculier's mining post]].
* In ''DrHorriblesSingAlongBlog'', the eponymous character has his own theme which plays during the act openings and as part of two songs. In one song, when he switches from philosophizing to EvilGloating halfway through, the background music also changes to his theme repeating until the song's end. In another song, a slowed-down version with heavy downbeats plays during a SuitUpOfDestiny sequence.
* In EverymanHYBRID, ''[[AxeCrazy HABIT]]'' has "Who Could Win a Rabbit" by AnimalCollective. And it's just as scary as he is.
* The Hitchhiker/[[spoiler:[[BigBad Malachite]]]] from ''SuburbanKnights'' has a menacing, drum-based one that plays whenever he appears.
* In early episodes of ''WebVideo/AtopTheFourthWall'', the arrival of '90s Kid would be marked with "[[Music/WeirdAlYankovic Smells Like Nirvana]]" and it would often keep playing until he was cut away from. Later, it was swapped out for [[{{Nirvana}} the genuine article]] and is usually not used at all if he's appearing in a dramatic scene.
** The entity [[spoiler: Missingno.]] has "evil" or alternatively "satanic" from the RenAndStimpy soundtrack (though that bit was unintentional.)
* [[WebVideo/TheCinemaSnob Kung-Tai Ted]] and The Ninja-Style Dancer (From WebVideo/AtopTheFourthWall, above) seem to share "Jet-Set" by Alphaville.
* The utterly deranged version of [[WesternAnimation/MyLittlePonyFriendshipIsMagic Fluttershy]] depicted in ''FriendshipIsWitchcraft'' seems to be associated with "[[Disney/TheHunchbackOfNotreDame Hellfire]]".
* Frequent ''Radio/TrueCapitalist Radio'' caller 213 (the Ghetto Capitalist) is often accompanied by the sound of a crying baby[[note]]It's fake, don't worry[[/note]] as he trolls the the host Ghost. This often leads to Ghost telling him to "stop choking that damn kid".
* The title character of ''WebVideo/EightiesDan'' has "Take on Me".
* When Nash of WebVideo/WhatTheFuckIsWrongWithYou encounters rampant douchebaggery on a massive scale (summarily titled 'Douchequakes') he plays Gunther's ''Ding-Dong Song'' over clips of stuff collapsing, falling over, et cetera.
** One episode of WTFIWWY Live! was ''[[UpToEleven so bad]]'', he played the song over the live feed of the webcast.
* The tune that plays most over WebVideo/TheNostalgiaCritic's depressed moments is [[http://www.youtube.com/watch?v=aFZ7bBaYpcw this]] crushing piece of [[LonelyPianoMusic Lonely Violin Music]].
** As of his comeback, the character himself seems to have an instrumental version of "the Review Must go On" as his theme.
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