%% This list of examples has been alphabetized. Please add your example in the proper place. Thanks!

* Barnacle Jones and Max from ''Webcomic/OneOverZero''.
* Black Belt from ''Webcomic/EightBitTheater''. Mostly [[WriterRevolt done out of spite by Clevinger]] after fans inundated the message boards with theories on how and/or why BB could be revived. The comic solving the issue was titled [[http://www.nuklearpower.com/2005/11/10/episode-622-now-shut-up/ Now Shut Up.]]
* Kairi in the first season of ''Webcomic/AnsemRetort''. WordOfGod says if she ever comes back to life, he's officially run out of ideas.
* ''Webcomic/AwfulHospital'': [[spoiler:Jay has figured out how to invoke this]] in a setting where death is usually only slightly more permanent than a paper cut.
* ''Webcomic/BittersweetCandyBowl'' has killed off several of the fan characters. In particular, Carson is abandoned in the basement of an abandoned house after falling through the floor, his "friends" not caring enough to tell anyone what happened to him. Presumably, he starved to death. Kizuna's creator freaked out over the character's presence in the comic and asked the author to remove her, resulting in [[http://www.bittersweetcandybowl.com/c54.1/p1.html this.]]
* Ethan in ''Webcomic/CtrlAltDel'' by the hands of an explosion, after one more comic the strip began to change focus.
* In ''Webcomic/CommanderKitty'', despite a mild HopeSpot involving Mr. Socks getting his brain removed and eventually put back in, [[http://www.commanderkitty.com/2013/03/24/tough-talk/ this is eventually revealed to be the fate of Nin Wah's old crew at the hands of Zenith]].
* Lt. Col. Thomas Knowlton in ''Webcomic/TheDreamer''.
* In ''Webcomic/{{Earthsong}}'', "deaths" are usually a DisneyDeath--while it looks dire for Nanashi to stab someone in the chest, what ''really'' happens is that the Siderean element of the sword removes the person's soulstone and returns them to their home planet (although, in certain cases, they may go back to a death anyway). When Beluosus invades Haven, however, [[spoiler:Tengu is mortally wounded and ''asks'' to die rather than be sent back, a request Nanashi honors. Then Skogul kills Nanashi, and the author makes clear in TheRant that it is dead-dead. The Spinster is also killed. Other deaths include Morgan and Ulkruz, consumed by Beluosus, and the outlying members of Haven's Guard who had their soulstones ripped out of their chests.]]
* In ''Webcomic/ElGoonishShive'', despite the fact that they NeverFoundTheBody, Damien is [[http://www.egscomics.com/?date=2004-07-14 explicitly stated to never be coming back]] in the [[BonusMaterial Painted Black Special Features]] strips which constitute WordOfGod.
* ''{{Webcomic/Erfworld}}'' example: After Wanda gets the Arkenpliers and reveals its powers of 'decryption', Parson hopes that she can decrypt Bogroll, since he died in a HeroicSacrifice. This hope is quickly squashed when Wanda points out that his remains "were rather thoroughly obliterated."
* The character Bush was killed near the end of ''Exploitation Now!'' She started off as a minor character but after CerebusSyndrome set became one of the two protagonists.
* ''Webcomic/{{Homestuck}}'' appears to be fond of this, though because of how many ways there are to come back, it remains to be seen just who all is gone for good. The trolls used to die with alarming frequency, and both the kids' and trolls' guardians are all officially dead, with the exception of the post-scratch instance of Dad Egbert/Crocker.
** On the other hand, the fairly generous afterlife offered by the dream bubbles means that death is little more than a minor inconvenience. Though it still equates to putting them on a bus for all narrative purposes. It turns out, though, that dream bubbles [[DeaderThanDead can be destroyed]].
* Freddy from ''Webcomic/{{Horndog}}'', although he later [[BackFromTheDead came back]] [[RiseFromYourGrave as a zombie]].
* Juu from ''Inhuman''. Word of God even stated in the comments for the page "JUU IS DEAD. DEAD. HE IS DEAD. CORPSE. AND HE IS NOT COMING BACK SO DON'T THREATEN TO BEAT ME UP OVER IT."
* [[Webcomic/MegaTokyo Fred Gallagher]] [[WordOfGod confirmed]] the death of Tohya Miho in #1244, a DPD strip:
--> Yes, yes, Miho is no more, I know.
** [[LyingCreator He lied.]] [[HesJustHiding She was just hiding.]]
*** [[ThisIsUnforgivable Yurusenai.]]
* In ''Webcomic/TheOrderOfTheStick'', although as a general rule DeathIsCheap, this happens quite often.
** Lord Shojo was attempted to be resurrected, but apparently he refused to come back (in ''TabletopGame/DungeonsAndDragons'', resurrection spell only works if the subject is willing to come back).
** Miko is a special case: while there is no in-story proof, [[WordOfGod the author]] specifically said she won't come back.
** Therkla said she wouldn't come back just before she died.
** Kubota got killed DeaderThanDead when Vaarsuvius disintegrated him.
** Redcloak just had her own wights kill [[DarkChick Tsukiko]] and eat her body, and no body means no resurrection.
** [[SinisterMinister Malack]] and [[EvilTwin Nale]] are killed within a few panels of each other. The former was reduced to ash and the latter's corpse was disintegrated specifically to keep him from being resurrected, leaving little to no chance of either of them coming back.
** Crystal was resurrected once (or to be more precise, made into a sentient golem). Next time she was dumped into lava, making sure she won't come back ever.
* Angels and demons are told to not have any afterlife in ''Webcomic/SlightlyDamned''. WordOfGod says that the character in question was created completely from the dreamer's imagination, no BackFromTheDead involved.
* Faye [=MacIntire=] from ''Webcomic/SomethingPositive'' dies quietly in her sleep after skipping a day off work to spend with her loving husband, Fred.
* The trope itself became a huge component of the last book of ''Webcomic/{{Sonichu}}''. After the author changed a fan character into a woman (Simonla Rosechu) and included her in the comic, he was demanded to remove her multiple times. Eventually the author was extorted into believing that he would be sued if he did not remove her and by that time the demand had been upgraded to killing her off on-panel. Simonla is blown up by an exploding toilet.
* Dave Kelly was very fond of this trope in his comics. In ''Purple Pussy'' he killed off Shelly Squirrel, and all the characters expected she'd come back since this was just a comic. She didn't. And in his other comic, ''Living In Grey Town'', he had so many important characters killed off willy-nilly that he added in a counter for every time a character died. By the end there were only a handful left.