%% This list of examples has been alphabetized. Please add your example in the proper place. Thanks!
* ''Fanfic/AStudyInRegret'': Watson and Mary.
* ''[[Fanfic/AceCombatEquestriaChronicles Ace Combat: The Equestrian War]]'', the following major characters are killed during the story:
** The Ponies: Blueberry, Raindrops, Carrot Top.
** The Griffins: Vulture, Razor, Scythe, Slip, Night Raven, Axe, Echo, [[BigBad Red Cyclone]].
** [[FridgeHorror That's not counting over two hundred nameless fighters and/or (in ponies' case) civilians that were killed during the war]].
* ''FanFic/AGrowingAffection'' has a pretty big death count:
** Good Guys: Gai, Sasuke, Jiraiya, Gamabunta, Kankuro, Itachi, Danzo.
** Bad Guys: Karin, Deidara, Kabuto, Orochimaru, Manda, Madara, Kisame, Zetsu, Homura.
* ''FanFic/AlternateDestination'': Gaia [[HeroicSacrifice/FanFics gave up on her position]] of a powerful, immortal spirit to resurrect the dead protagonist, which ended up killing her in the long run. At the end of the story, when it seems everything has been undone, we learn that she's NOT coming back.
* ''FanFic/AvatarTheLastAlicorn'': Littlewing and the illusionist both died, along with whatever was left of Sombra.
* ''Fanfic/CalvinAndHobbesTheSeries'': The fifth season premiere reveals that [[HardLight Holographic]] [[BigBad Retro]] didn't make the previous season's {{cliffhanger}}.
* ''Fanfic/{{HERZ}}'':
** In chapter 10 Gendo got shot by a SEELE member and died in Shinji's arms.
** All SEELE members in the last chapter, turned into LCL by Rei.
* ''FanFic/ClashOfTheElements'': In Part 1, All the Outer Guardians Minus Driad, the Genesis Samurais (Physically anyways, and Joe Dark
** In Movie 1, Tabuu
** In Part 2, All of Smithy's creations minus Axem Red, Smithy himself, Dimentio, Cackletta, Chaos Alex, and Alex Whiter
** In the What-Ifs, Not counting the millions of people that are erased in the cataclysmic events that unfold we got Alex Whiter and Joe Dark/Dark King in Demise Under the Stars and Alex Whiter, Tabuu and Cackletta in Return to a Blank Slate
* In ''FanFic/DespairsLastResort'', Kaito, Naomi, Shigeru, Shizuka, Miyako, and Saemi are confirmed to be dead forever due to the damage caused during their deaths in the simulation.
* Buffy in the [[Series/BuffyTheVampireSlayer BtVS]][=/=]ComicBook/{{X-Men}} fic, [[http://www.tthfanfic.org/Story-19569/Chilord+Echoes+of+the+Fallen.htm Echoes of the Fallen.]]
* ''Manga/{{Evangelion 303}}'': Jessika died when Unit-04 crashed. There are no doubts about it, since they found her head.
* In addition to many OriginalCharacters throughout, chapter 2-6 of ''[[FanFic/IAgainstIMeAgainstYou I Against I, Me Against You]]'' confirms the death of Derpy Hooves.
* Discord and, [[http://www.fanfiction.net/s/9026708/16/My-Brave-Pony-Starfleet-Magic-III as of Starfleet Magic III, Twilight Sparkle]] in ''Fanfic/MyLittleUnicorn''.
* Caliborn in Fanfic/JakeEnglishsMysteriousTheaterOfScientificRomanceFromTheYear3000.
* Jian Feng and Prince Adachi die at the end of the first arc of ''FanFic/KyoshiRising''.
* In ''FanFic/OriginStory'', Bullseye, the Radioactive Man, and Venom all die badly, because they hurt Louise, and no one hurts Alex's loved ones and walks away. [[ObstructiveBureaucrat Henry Peter Gyrich]] [[TooDumbToLive doesn't learn from their example]].
* In ''Fanfic/PerfectionIsOverrated'', the death toll includes all of the [[ParodySue SUEs]], Kyoko, Ishigami, Yukariko, Miyu, Nagi, The Usurper and The Obsidian Lord. Unlike in [[Anime/MaiHime canon]], anyone who dies here stays dead, as a result of Miyu's destruction late in the fic.
* In ''FanFic/QueenOfAllOni'', a JackieChanAdventures fanfic, Daolon Wong dies in prison, and his apprentice Lung is later killed in a classic HoistByHisOwnPetard moment: he captures Jade, trying to break her will and control her, but is killed when her bodyguards show up and [[CurbStompBattle MOW through his defenses]], also an example of LaserGuidedKarma, since he nearly KILLED Jade, as he underestimated how stubborn she was.
* The ''Fanfic/PonyPOVSeries'' has a relatively small death count:
** In the main timeline, Shining Armor's friends Sunset and Private Ranger are all killed in action while stopping a mega storm.
** In the [[BadFuture Dark World Arc]], it being a thousand years later, nearly everyone who wasn't immortal (or made immortal by Discord) is long dead. However, at the end Rarity's newly ascended Alicorn self, Queen Libra, is allowed to resurrect everyone who was killed by Discord or his forces, but due to the rules, some DO stay dead. Big Mac died of old age and Pinkie Pie and Fluttershy both died well after their natural life span, for example, so they ''do'' stay dead. Cadence was also killed in a LastStand and didn't return, but she's a goddess so she is simply helping from the afterlife. Queen Chrysalis was also absorbed by Cadence and is likewise dead for good.
* Viral, in ''FanFic/TengenToppaGurrenSolvernia''.
* ''FanFic/TheBully'': Iona [=MacEachern=] and her boyfriend Craig [=Sullivan=] were drowned by their swimming teacher in "Harry Smith's Swimming Lesson", Steven [=McMullen=] received a "[[TitleDrop poisonous government injection]]" in "Harry Smith's Poisonous Government Injection" and four characters were killed by Euan [=Milton=] in "The Destruction of Colham High School".
* From ''Fanfic/YoungJusticeDarknessFalls'': We have Vandal Savage, Glorious Godfrey, Volcanna, Doomsday, Klarion. A pretty high body count considering the canon show was a bit more conservative with its deaths. In additions, several heroes are either NotQuiteDead or come BackFromTheDead, meaning there's less of a net loss than this list might indicate.
** FanFic/YoungJusticeTitans'' adds to the pile Kid Devil, The Riddler and Vibe.
* From the ''Gensokyo20XX'' series:
** 20XX and 20XXI: Youmu, Cirno, Daiyousei, Remilia, Wriggle, Reisin, Shinki, Alice, Marisa, Sanae, Suwako, Flandre, and Eirin.
** 20XXII: Yuugi, Keine, and Ran's babies are revealed to have passed away and Aya dies some time during.
** 20XXIII: Meiling and Patchouli, along with Seija in a KarmicDeath
** 20XXIV: Sakuya, Reimu's mother, Ran's babies (save Yume Ni and An). As of chapter 50 in Gensokyo 20XXIV, some of those characters were brought back from the dead, through Yukari bending some of the borders of time to bring some of them to the future.
** 20XXV: Koishi, Satori, Yume Ni, An (being revealed to be DeadAllAlong), and, apparently, as of chapter 109, Ren. Along that line, it is implied that Yuuka has passed away and, by the 120th chapter, Kosuzu is mentioned to be "long gone", implying that she had died a long time prior.