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* Misuzu in ''VisualNovel/{{Air}}'', in one of the most [[TearJerker heart-wrenching]] anime scenes ever devised (the word "goal" will never hold the same meaning anymore to anyone who has seen it). The fact that she ''might'' be reincarnated or freed from her curse does not diminish the fact that Misuzu herself is gone for good and leaves behind her immensely grieving adoptive mother, Haruko.
* ''Animation/BlackCatDetective'' is almost schizophrenic about this. On the one hand, this show has no problem killing characters for real. The evil mice [[CaptainObvious certainly die when they get killed]]. His second in command, well, if you watch this show, don't get to attached to him. But the elephant who gets shot in the middle of the head at point blank range is revived by an enormous syringe.
* Cibo and Sana-Kan from ''{{Blame}}''! cop it for real at the end of the series. This comes as quite a shock, as both characters had [[DeathIsCheap technically died several times before this]]. Admittedly, Sana-Kan's death ''is'' heavily {{lampshade|Hanging}}d during a conversation with her [[TheOmniscientCouncilOfVagueness apparent superior]].
* After years of EverybodyLives for the protagonists in ''Anime/{{Bleach}}'', this trope finally comes in the Thousand-Year Blood War Arc. Confirmed by WordOfGod that he is no longer pulling punches in killing off some of even fan favorite characters.
** List of Soul Society casualties:
*** [[spoiler: Lieutenant Chojiro Sakisabe]]
*** [[spoiler: Old Man Yamamoto]]
*** [[spoiler: Restu Unohana aka Yachiru Unohana]]
* This is an inviolable rule in the Creator/{{CLAMP}} universe. If a person dies, there is absolutely '''NO''' way to bring back him back to life '''EVER'''. In some mangas it is also hinted that not even ''gods'' have the power to bring the dead back to life. The plot of ''TsubasaReservoirChronicle'' comes from the problems that result from trying to circumvent this rule. The rule only applies to living beings who have a "natural existence". So, Chii is excluded from the rule. Even C!Shaoran and C!Sakura can be "brought back from the dead", because their "birth" is "unnatural".
* Played straight ''and'' subverted in ''Anime/CodeGeass''. One of the elements contributing to its BrokenBase status was the fact that sometimes a character would appear to be Killed Off for Real, explicitly or at least implicitly, when in fact it was a case of OnlyMostlyDead (Jeremiah, Mao, Guilford, and Cornelia) or NeverFoundTheBody (Nunnally, Suzaku). The characters who DO die for real include, among others, Clovis, Mao (the second time), [[http://www.youtube.com/watch?v=d-UV1sk8ZNo Euphemia]], Shirley, [[http://www.youtube.com/watch?v=0vrhIyYrJ4k Rolo]], Emperor [[http://www.youtube.com/watch?v=2sSAD4FFpPw Charles, Marianne]], Diethard and, finally (possibly), Lelouch.
** WordOfGod says Lelouch has indeed been killed off for real.
* ''Manga/DeathNote'' is set in the Real World. [[CaptainObvious When somebody dies, they stay dead]]. It is initially implied that, for Death Note users, when they die, instead of Heaven or Hell, they go to Nothingess (Mu). [[TheNothingAfterDeath Ouch.]] It is later revealed that this is true for EVERYONE.
** Not that this has stopped the fanfic writers.
** In the manga, Light uses deductive reasoning to come to the conclusion there is no heaven or hell.
* Leomon from ''Franchise/{{Digimon}}'' [[Anime/DigimonAdventure season one]] and [[Anime/DigimonTamers three]]. Also Oikawa, Black Wargreymon (could not be reborn due to being artificial), Arukenimon, and Mummymon from season two, as well as some family members of various characters that died before the seasons' start.
** We might not see him again, but Leomon (Season 1 only) was presumably reborn, as Whamon was. Digimon who are destroyed get reborn in Primary Village. Except the evil Digimon, cause they never come back to cause trouble after they are destroyed (presumably sent to the Dark Area where the Digimon Emperor briefly retrieved Devimon's soul from). Etemon's death was retconned and Myotismon was revived, but both are later killed for good. This only applies to Seasons 1 and 2; 3 is a different type of universe and it does not apply. Wizardmon, on the other hand, wasn't reborn (possibly because he died in the real world) and returned as a ghost to help them defeat the Digimon Emperor, and then disappeared.
*** ''DigimonTamers'' counts as a meta example,all deaths are final as opposed to every other series where everyone can be reborn. It just so happened that Leomon was the first time a main characters Digimon got killed off.
**** The only exception is Vajramon who comes back for no reason the first time but is destroyed for good the second time.
*** Wizardmon's death was, in fact, permanent. Because he died in the 'real world', there was no way for his data to be reborn.
*** Pumpkinmon and Gotsumon also got Killed Off for Real. That "dungeon" crap was just DubText; they clearly got killed in the real world just like Wizardmon courtesy of Myotismon's BadBoss tendencies.
*** In a sense, Devimon returns as a ghost, but he is destroyed for the second time along with Kimeramon.
** ''Anime/DigimonSavers'' also works sort of like the first two seasons until Kurata figured out how to permanently kill off Digimon, which he did to [[SacrificialLamb Frigimon]], [[NobleDemon Merukimon]], Eldradimon, [[AssholeVictim SaberLeomon]], and countless others.
* Anime/DragonBall, the franchise that consistently proves that DeathIsCheap, has succeeded in killing off several minor characters. Not focusing on the swaths of people that were killed that couldn't possibly have been brought back by the Dragon Balls, you also have several of the Kais. They were absorbed by Majin Buu, but due to being within him for millions of years, there's no way to remove them from his body. So when Buu dies, they officially die as well.
** Frieza, Cell, and Buu, the main antagonists of Dragonball Z, are notable for surviving several attacks that seemingly kill them. Frieza survives Goku's Spirit Bomb, and later survives an energy blast from Goku that he and King Kai think killed him, and then Namek's explosion afterward. He returns as a cyborg, but is destroyed for good by Trunks. Cell can regenerate lost limbs, most notable instances being when Goku destroys his top half and when he selfdestructs, but in the end Gohan destroys him. As if Frieza being reduced to dust and Cell's energy vanishing completely wasn't enough, both are later shown in HFIL (Hell) with halos over their heads, further proving that they are dead. And no matter how often Buu is blown to bits, he always comes back together, but is finally destroyed by Goku with a Spirit Bomb.
** Android #16 is the only good guy who is killed and never comes back to life.
* ''VisualNovel/FateStayNight'' has the protagonist, Shirou ([[MultipleEndings potentially]]) [[HeroicSacrifice killed off]] in the finale of Heaven's Feel. The TearJerker ending sticks to and expands on this, showing the epilogue through the eyes of Sakura as she ages and watches everything change around her, remaining alone until the end... The most depressing part of the game, hands down.
* The final deaths of all the homunculi except Pride in ''Manga/FullmetalAlchemist''. Hughes, Fu, Buccaneer, Kimblee and Hohenheim die as well, and the manga makes it very apparent [[AllDeathsFinal that they cannot possibly come back]].
* The ''GetterRobo'' franchise has Musashi Tomoe, who in nearly every continuity dies in some form of awesome HeroicSacrifice.
* The entire Franchise/{{Gundam}} franchise is absolutely ''notorious'' for this. With only a few exceptions, all of the series pull no punches when displaying the brutality of war. This is the series that gave rise to KillEmAll after all.
** The Universal Century timeline has this all over the place. Besides the millions upon millions who die in the background, major characters fall in some fantastically '''brutal''' ways. The most memorable of these comes during CharsCounterattack, where [[TheHero Amuro Ray]] and [[TheRival Char Aznable]] die at each other's hand in the climax.
** The Cosmic Era is hands down the [[BloodierAndGorier bloodiest]] and [[DarkerAndEdgier darkest]] of the [[AlternateUniverse Non-UC timelines]]. ''[[Anime/MobileSuitGundamSeed Gundam SEED]]'' in particular saw the death of more main characters than the most violent of the Universal Century series, ''[[Anime/MobileSuitVictoryGundam Victory Gundam]]''. Thanks to higher technological capabilities at the time of production, all of the carnage was visible in high definition.
* The original Lockon Stratos was Killed Off for Real near the end of the first season in ''[[Anime/MobileSuitGundam00 Gundam 00]]''. [[http://www.youtube.com/watch?v=DSraFV8AOvM It's arguably the most tragic moment in the whole series]].
** Just when they're getting developed, Gundam 00 also has the deaths of both Lichty and Christina via point-blank gunshot from a ''mobile suit''. It's made all the more tragic considering Lichty [[HeroicSacrifice used his body]] to shield Christina, but she got a large piece of debris jabbed into her back anyway. Just in case there was any thoughts they could survive, ''the cockpit also exploded''. This series is obviously fond of deaths by explosions.
*** And then there death of Ali Al-Sachez near the end of the second season. The original Lockon's brother, Lyle, tried to spare him but when Ali tried to use the opportunity to kill him, Lyle quickdrew his pistol and shot him in the head then casually shot him a few more times in the torso to make sure he was finished.
*** On the other hand, Tieria was shot similarly a while earlier but [[VirtualGhost survived]] and came back in TheMovie to kick some more ass... only to die ''again'' when his Gundam went into [[SuperMode Trans-Am]] [[ExplosiveOverclocking overload]] to avoid [[YouWillBeAssimilated being assimilated]]. He didn't stay dead this time either, unlike [[http://www.youtube.com/watch?v=gTREnsljqWk Graham]] and [[http://www.youtube.com/watch?v=80vHif4TldY Andrei]].
** And let's not forget Nena. She got her [[SoLastSeason antique]] mobile suit brutally dismembered into just the cockpit by Louise, who then stabbed the cockpit for good measure. Naturally, the last we see of Nena is of her cursing the hell out of her opponent with BloodFromTheMouth before her cockpit explodes. Conversely, her brothers suffered brutal fates as well, with Michael suffering a OneHitKill and Johann lamenting as his unit is being destroyed.
* ''Manga/InuYasha'': There are several methods for returning the dead back to life and several characters that die end up bring brought back to life (a couple of characters even die twice and are restored to life twice). When [[CameBackWrong Kikyou]] and [[BrokenBird Kagura]] end up dying in RedemptionEqualsDeath storylines, the in-universe methods that exist can't save them as both of them end up being a case of NoBodyLeftBehind. Subverted with [[DesignatedVictim Kohaku]]. He is set up to be this trope when it's confirmed the known methods for restoring the dead to life won't work on him. However, when it finally happens, a new one-off method turns up to restore his life, after all.
* The mangaka of ''Manga/JoJosBizarreAdventure'' is not afraid to kill off his characters. Supporting protagonists usually have a good chance of dying; William Zeppeli from Part 1, Caesar Zeppeli from Part 2, and several of the primary protagonists' TrueCompanions from Parts 3 and 5. Mostly averted in Part 4, which takes place in relatively tame circumstances compared to the other installments.
** Even main characters can get this treatment when you least expect it. Jonathon Joestar sacrifices himself at the end of Part 1, Jolyne Kujo (along with ''everyone else on the good guys' side'' save for their TagalongKid) are killed just before the conclusion of Part 6, and Gyro Zeppeli (who shares main character status with Johnny Joestar) dies in Part 7.
* ''{{Kekkaishi}}'''s Gen, much to the surprise of... everyone.
* ''KenichiTheMightiestDisciple'' has this happen to a few important characters throughout the story.
** One of these is Sho Kanou, Kenichi's YOMI rival in the D of D TournamentArc, who pulls a HeroicSacrifice by jumping in the way of gunfire meant for Miu; his death affects the characters so much that Kenichi keeps [[TragicKeepsake Sho's birdcage earring]] as a reminder of his promise to Sho [[IWantMyBelovedToBeHappy to always protect Miu]], and Sho's master Akira Hongo (the "God Fist" of the One Shadow Nine Fists) is revealed to be a FatherToHisMen and a JerkWithAHeartOfGold who didn't take his disciple's death very well and later expresses a wish that no more disciples should have to lose their lives.
** Another character who gets this treatment is Silcardo Junazard, the "Demon Fist" of the One Shadow Nine Fists. In this case it's a badly-needed case of KillHimAlready, he violates the unofficial rules which stipulate that master-class fighters must not interfere in fights between two other masters and (more seriously) must not meddle in fights between two disciples, and crosses the MoralEventHorizon by manipulating Akira Hongo into almost killing Kenichi--''almost'' because Hongo, who was fighting unconsciously at that time, realized what was about to happen and pulled back on his attack at the very last second--all so he can take advantage of Miu's resulting sense of mental chaos when she thinks Kenichi's been killed, kidnap her and brainwash her to become his newest student. In fact, the character is so bad in this regard that he ends up getting an utterly satisfying KarmicDeath at the hands of a vengeful Hongo during an EnemyMine with Ryozanpaku, possibly with sanction from the One Shadow ([[PapaWolf Miu's father]]) himself.
* Everyone who was dear to, or trusted by, the protagonist of ''LeChevalierDEon'' is dead by the end of the series -- [[DownerEnding leaving him completely alone and utterly disconsolate.]]
* In the ''[[SuperDimensionFortressMacross Macross]]/{{Robotech}}'' episode, "Pineapple Salad/Goodbye Big Brother," it should have been made obvious what was going to happen with Hikaru Ichijou/Rick Hunter's senpai/brother, Roy [[SpellMyNameWithAnS Focker/Fokker]], but the usual standard {{bowdlerization}} of 1980s television animation made assuming that the show would choke rather obvious. When the show actually followed through and killed him off, it showed this was no ordinary animated series on North American TV. MoralGuardians raised a fuss and complained that it was bad for children.
** No such ambiguity in the original Japanese version of the episode. Three ragged holes in his back and everything. And the reactions of the rest of the main characters seals the fact that he wasn't coming back. In fact, just to give Akira Kamiya more stick time as Focker, they had to bring him back in a prequel.
** After pulling off a couple fakeout deaths and at least one deliberate [[MythologyGag death joke]], ''MacrossFrontier'' may have gone overboard in making sure viewers understood that Michael Blanc was dead. He gets stabbed in the chest with an alien claw that's almost as big as he is, and is then sucked out into hard vacuum through a self-sealing hull breach. No real way of coming back from ''that'' one.
** You forgot the 'coughs up blood, makes a final 'I love you' speech, does the ReallyDeadMontage and has a memorial shot at the end with his glasses. You know, because [[Film/{{Aliens}} it's the only way to be sure.]]
* Non-minor characters from ''MagicalGirlLyricalNanoha'' that [[SlidingScaleOfIdealismVersusCynicism somehow managed to die]] instead of simply getting [[DefeatMeansFriendship befriended]]: The first Reinforce, Zest. Non-minor characters from ''MagicalGirlLyricalNanoha'' that seem to be dead: Precia. Reinforce, in particular, is shown to be doomed to die even in [[VideoGame/MagicalGirlLyricalNanohaAsPortable continuities where she survives past her original time of death]], even though other characters related to the Book of Darkness, such as the Wolkenritter and the Materials, would prove to be especially resilient to death, surviving several {{Near Death Experience}}s and [[BackFromTheDead death itself]], and Reinforce herself was the first character described as unkillable due to her Rejuvenation Program.
** Due is the only one of the twelve Numbers who was killed in the series, while the others went either a HeelFaceTurn or went into prison. Unfortunately, Due was the last Number to be introduced, had the least screen-time among the sisters, she was one of the two most evil Numbers, [[ArsonMurderAndJaywalking and she was the only one with a seiyuu who didn't have double roles in this series]].
* ''MartianSuccessorNadesico'' killed off Akito's friend and enthusiastic mecha pilot Guy Daigoji early on, and teased his return several times. The only time he ever appeared again was in a hallucination, and it wasn't Akito's. The character that ''did'' come back was a minor backgrounder. Another example of the show's recurring theme, "life is not like SuperRobot anime".
* While ''Manga/{{Naruto}}'' has several [[DisneyDeath Disney Deaths]], it also has several examples of this trope, including The Third Hokage, Asuma, Jiraiya, Itachi, Pain, and Danzo to name a few. Some characters like Asuma and Itachi are brought back to life to fight in the Fourth Great Shinobi War, but they are closer to zombies than how they were before they died.
** Shikaku, Inoichi & Ao - as well as everyone within the HQ of the Allied Shinobi Forces when it was obliterated by the Ten-Tails.
** Neji's death is certainly here as well. To further prove the death, his curse mark disappears, which only happens when a Hyuuga dies. So no subverting the DisneyDeath here.
* ''Anime/NeonGenesisEvangelion'': depending on which ending you choose, either Kaji and Kaworu, or [[KillEmAll the entire human race except for Shinji and Asuka]]. [[MindScrew Maybe]]. Depending on how you interpret the original ending, possibly the entire human race period.
* There's a common rule in ''OnePiece'': If you die in a flashback, you're Killed Off for Real. If you don't die in a flashback, chances are, you might pull off a DisneyDeath. Prime examples of the former are Sabo, Bellemere, Hiruluk, Tom, Kuina, Usopp's mother, Robin's friends and mother, and Brook's pirate crew. However, the latter rule saw itself subverted, with Portgas D. Ace having fallen straight into this, and Whitebeard following two chapters later.
** Following these, the rule seems to have expired: In Punk Hazard, two of the antagonists, Monet and Vergo, were killed.
** Sabo is an exception to the flash-back rule as chapter 731 reveals that he did survive and is now a revolutionary.
* Where do we EVEN begin with ''Manga/PandoraHearts''? First we have Break's entire family he served as a knight wiped out by chains, then the Flower Girl, Phillipe West's Dad, Human Alice, her soul is still alive, she just doesn't have a physical existence after Oz rejected her and sent her to the Abyss, Oswald (dismembered by Oz when he was B-rabbit,), Lacie, who was sacrificed to the Abyss and is DeaderThanDead, Arthur Barma, Isla Yura and his cult, Elliot Nightray and his entire family save for Gil and Vincent, Cheshire Cat (although he's alive still),Rytas and her master and Gruner, Reim he lived. His chain can fake death, but it still leaves the user with a bunch of injuries., Jack, who was killed by Glen 100 years ago He's alive, his soul is just shattered into bits after being forced to live through a GroundhogDayLoop of his body de-aging over and over., Revis., Oz is thought this way to people who don't know he got out of Abyss. Shot by Gil, but he lived. Damn, why do so many characters die in this series?
* In ''Pokémon'', the scientists who created Mewtwo in the first movie, Latios in the fifth movie, Lucario and Sir Aaron in the eighth movie, and J in [=DP151=].
** Also in the episode directly after J's death, what happens to Cyrus when he enters a portal to a dimension that is then destroyed by Dialga and Palkia?
* ''Anime/PuellaMagiMadokaMagica'' killed off Sayaka while the protagonist, Madoka, willingly erases her existence from both the physical world and the memories of (almost) everyone who knew her. Mami and Kyoko were seemingly Killed Off for Real only to be brought back in the final episode.
* In ''{{Rainbow|NishaRokubouNoShichinin}}'', Sakuragi dies about midway through the series. This is especially surprising since he was TheHero, BigGood and possibly the protagonist as well.
* Most of the cast of ''Manga/RoseOfVersailles'' are killed off at the end of the series (save for Rosalie, Bernard and Alain). Since it took place in TheFrenchRevolution, this is [[ForegoneConclusion a given]].
* ''{{Simoun}}'' has the HeroicSacrifice death of Mamina. And to add insult to injury, the Simile carrying her coffin is shot down. Other characters are much more ambiguous: We have Rimone and Dominura, who TimeTravel, then take off to parts unknown; Amuria, for whom they NeverFoundTheBody; Onasia, who, well, [[MindScrew who knows]]; Yun, who now exists in the sort of limbo the previous did; and Neviril and Aer who AscendToAHigherPlaneOfExistence. Angulas also manages to die as a suicide bomber, but still show up as a body in the cockpit of an enemy Simoun later. ''Great'' series, but very very strange.
* The character Cosmo is killed in the last episode of ''SonicX''. By a shot fired by Tails, using a cannon for which Sonic and Shadow were the ammo, no less. Though this is [[{{Macekre}} completely wiped out in the dub]].
** The same is true for the revolutionary, Molly, in the episode ''Molly's Dream''. 4Kids even went so far as to digitally edit out her gravemarker in the final scene.
** Emerl is also killed by Cream after she fails to bring him back to normal at the end of the Sonic Battle arc, since Gemerl doesn't exist in Sonic X he ends up as this.
* Everyone in ''SoukouNoStrain''.
* In ''Manga/SoulEater'' manga there Mifune, Tezca, Arachne and, Joe.
** Though one of these deaths was faked to get cover.
*** Not as of chapter 86...
*** Tezca is a bizarre case because he seems to be somehow immortal. After he dies, his soul is still alive, can still appear in mirrors, implies that he can just get a new body, and still counts himself as being one of the Death Scythes.
** Shinigami in ch109. Kid doesn't realise this until he returns from the final battle with Asura, and Excalibur 'helpfully' explains why it's all Kid's fault.
* Kamina's death in ''Anime/TengenToppaGurrenLagann''. And Kittan, Nia, Balinbou... AndTheRest. Though those were near the GrandFinale, so their deaths weren't quite as shocking as Kamina's, who died in the eighth episode and was featured as a really important character.
** ''Lagann-Hen'' averted this by keeping the rest of Team Dai-Gurren (save for Kittan) alive until the final battle.
* In a subversion of NeverSayDie, in the English dub of ''Yu-Gi-Oh!'', Cecelia Pegasus is killed by an illness (prompting Pegasus to begin Duelist Kingdom as an elaborate ruse thrown together at the last minute so that he can get his hands on Yugi's Millennium Puzzle and resurrect her), Gozaburo and the Big Five are all destroyed along with the virtual world they are trapped in as it is destroyed (Noah isn't listed here because he is speculated to have saved his mind on a backup file, whether or not he did, and therefore invokes NeverSayDie), and also Yami Marik, the Great Leviathan, Yami Bakura, and Zorc are all destroyed near the end of their arcs (although [[WhatMeasureIsANonHuman how far these four can be subverted to NeverDayDie is debatable]]. Ahmet is also eaten by a monster in a flashback during Season 5.
** Divine was truly killed in ''Anime/YuGiOh5Ds'', devoured by Earthbound God Ccarayhua (and strangely, the dub did very little to sugarcoat this). Divine clearly did not deserve to be spared; as far as [[AssholeVictim Asshole Victims]] went, he was one of the biggest.
*** Divine was actually not killed. Word of God has proven that he is in jail. His being "eaten" was akin to all the human souls that were absorbed by the Earthbound Gods/Immortals. Thus, they returned at the end of the arc. The real "killed off for real" characters in that arc are Rex and Rudger/Roman. After that, it's every person aside from Zone in the apocalyptic future.
**** And then Zone himself.
** In the original version, Edo Phoenix's adoptive father DD perished in his final confrontation with Edo (although the dub version had him make a threat which made his fate ambiguous).