!! Film
* Dammit, Janet. Brad's devotion towards Janet is quite sweet...Too bad things take a downhill turn from there.
** The fact that their first instinct after getting engaged is to visit the professor who made them get together in the first place, presumably to tell him the good news and thank him.
* Another is when Brad stands up to Frank just before they are turned to stone, defending Janet even after all her betrayal. Many Janets performing live will react to this Heartwarming Moment even though no such reaction is onscreen.
** Dr. Scott, too, will stand up to Frank after Brad gets TakenForGranite, telling him not to hurt Janet. It...stops nothing, but it's the thought that counts.
* Rocky crying after Frank dies.[[note]][[AudienceParticipation You broke my transvestite!]][[/note]] He'd been treated like nothing more than a sex object for the entirety of his eight-hour life, but Frank was still the closest thing he ever had to a father figure.
* The combination of "I'm Going Home"/"Don't Dream It" has become an anthem both for LGBTQ+ fans and all the misfits out there who long for acceptance, who interpret the songs as a call to embrace their true selves and find community.

!! Stage Show
* Unlike in the movie, where Rocky is portrayed as childlike and naive, he and Janet actually have a talk about all the torture and assault they went through. This segues into Janet asking him to touch her.
* Columbia dies from jumping into the first laser shot to protect Frank, even after the crap he had done to her.
** Frank's reaction to it is pretty heartwarming since he is genuinely shocked that she'd sacrifice herself for him. Sure, his reaction afterwards is pretty {{Jerkass}}, but you can tell part of him didn't want her to die.
-->'''Frank''': (After Columbia dies) Stupid bitch.
* Depending on how sympathetic you are to Magenta and Riff Raff after they kill Frank, hearing Magenta nostalgically reminisce about their home planet, knowing that they'll soon be bac], especially when the movie shows earlier clips of her and Riff dancing happily to "The Time Warp."

!! 2016 Remake
* When Brad proposes to Janet, she has trouble getting her gloves off due to being so excited. She has to pull it off with her teeth before accepting the ring.
* The standing ovation for Tim Curry from the theater.
* Brad and Janet hug each other in fright during "Time Warp" as everyone dances around them. Brad is less condescending towards Janet, admitting that he's as scared as she is but they need to find a phone or they can't leave.
* To Dr. F's credit, in this version she doesn't force either Brad or Janet to have sex with her. They figure out the BedTrick rather quickly after she strokes them, and they try to get away. Tim Curry's Dr. F. visibly pinned both parties down, but Laverne Cox's version is more verbally manipulative.
* During "Wise Up, Janet Weiss," Brad in this version has the sense to not bring along Dr. Scott to try and wheel him. Instead, he follows at breakneck speed to rescue Janet from Dr. Frankenfurter. The problem is that Dr. F. reveals they were LuredIntoATrap. Brad then shouts at Dr. Frankenfurter to not hurt Janet; she looks touched and horrified when Dr. F turns Brad and Dr. Scott into statues.
* After Brad, Janet, and Dr. Scott escape with their lives, they take a few minutes to sing about how they don't know how to deal with the aftermath. Brad and Janet then help Dr. Scott into his wheelchair, and they get away from the theater.
* Tim Curry's rendition of the finale song is more contemplative than depressing. He talks about how we are insects, and yet lives are not fleeting.

!! Other
* Despite having suffered a crippling stroke, Tim Curry still took part in the 2020 Live Read. While he noticeably had difficulties keeping up, Nell Campbell can frequently be seen filling in gaps between his lines and genuinely seeming very supportive.
* Columbia and Meat Loaf dancing together during his music number is pretty sweet, especially since it's the most happy Columbia is during the story. Too bad Frank has to ruin it.