* The fact that the sixteen ninjas' [[TrueCompanions friendship]] transcended lifetimes, and that most of them were friends already.
* [[spoiler:Tsunade]] considers Chopper her son, and won't leave Drum Island without him.
* Doubles as TearJerker. [[spoiler:Sai's last request to his granddaughter is that should she ever meet the reincarnations of the sixteen ninjas, who were his first and closest friends until their deaths, that she'd tell them about how he lived his life]].