* Nearly any scene involving Major Victory ([[{{Tearjerker}} with a few definite exceptions]]).
** One particular favorite came in the very first episode. The child actress playing the lost girl the heroes are meant to be helping makes a very visible WTF Face as Major Victory insists upon carrying her, Superman style, to the nearby police station.
** [[LargeHam "WHO IS THIS GIRL'S MOTHER?"]]
** He runs past a bunch of birds milling about, and as he does, points to them and grins in an overly cheesy fashion, all without stopping.
** "Today, I am a wiener. Not a winner."
** "I gave away my secret identity! I have a new name...Major Dumbass!"
** After the CrowningMomentOfHeartwarming of him receiving a call from his estranged daughter, he looks up at Stan and jokingly asks if he can keep the phone that Stan provided for the call. Stan has a good laugh at that one.
** "I know Major Victory can't keep his costume on. But ''[[ChippendalesDancers Thong Man]]'', now we haven't been introduced to that particular superhero."
*** Major Victory tells Dark Enforcer that he's going down for this. Dark Enforcer responds by waving a dollar in front of the screen.
* Other sources of comedy in the first season:
** Monkey Woman. The name says it all, really.
** Ty'veculus, due to the [[ButtMonkey sheer amount of setbacks he keeps enduring.]]
** The Dark Enforcer, who has many a DeadpanSnarker moment.
* And in the second season:
** Ms. Limelight, who is TheDitz to hilarious levels.
** Mr. Mitzvah, during both challenges and eliminations.
** Hyperstrike, a young and energetic LargeHam with acrobatic skills.
** Parthenon, once he puts himself through {{Flanderization}} into CampGay.
** SmugSnake Dr. Dark and his creation: "Evil Stan Lee".
* HOOOOOOOOOOOOOOOOOOOOMELESSSSSSSSSSSS MAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNN!
** I HAVE THE POWER TO BELIEVE I AM FLYING!