[[quoteright:276:https://static.tvtropes.org/pmwiki/pub/images/detana_twinbee_funny_moment_6.png]]
[[caption-width-right:276:Whoops! I caught the mad scientist.]]

* The [[ItMakesSenseInContext out-of-context]] page picture of the main characters catching a mad scientist altogether by literally fishing him out. Certainly an instance of SurrealHumor.
* The first few notes of the intro of the [[https://www.youtube.com/watch?v=7gMKk_5rnxo NES]] or [[https://www.youtube.com/watch?v=AunXucs23DQ arcade]] game will have you thinking, [[Franchise/{{Superman}} "Is it a bird? Is it a plane?"]] as the [=TwinBee=] ships fly in, with the implied conclusion being, "No, it's [=TwinBee=]!"
* As for the melody used against boss fights for the [[https://www.youtube.com/watch?v=28s2Afg2Neo Famicom]] or [[https://www.youtube.com/watch?v=NrFAAqikqkQ arcade game]], it's humorously reminiscent of another familiar [[Film/{{Jaws}} movie's]] [[JawsAttackParody musical sting]].
* The mere idea of having to fight [[EverythingTryingToKillYou things]] like flying strawberries, turnips, snowflakes, cups, plates, silverware, eggplants, and crustacean like you're dealing with a shoot 'em up version of [[WesternAnimation/BeautyAndTheBeast "Be Our Guest"]] with ships that have [[FingerlessHands boxing-glove hands]] is hilarious in its own right.
* If you shoot and juggle the bell power-ups with enough bullets for a long enough period of time, they turn into [[WorldOfChaos outright bees]].
** However, HilarityEnsues when you try to fly towards them to absorb them, since they tend to [[CollisionDamage kill you]] upon contact like the regular enemies would.
** You have to shoot the bells-turned-bees like regular enemies to kill them and turn them into points that add to your score. Many a first-time player has been [[SchmuckBait victimized]] by these infamous [[BonusFeatureFailure bonus item bees]].
* In the MSX version of ''[=TwinBee=]'', you get a [[ColourCodedForYourConvenience black bell]] instead of the bee if you shoot the bell enough times. Avoid it, because that bell will also [[PoisonMushroom kill you]] when you attempt to [[SchmuckBait absorb]] it like the other bells. However, you can't shoot it to kill it like the bees.
* The [[SprintShoes blue bell]] is one of the most basic and useful items in the game and can speed you up to five levels. However, it's best you stop at three levels, or [[PowerUpLetdown else]] you'll end up TooFastToStop.
* This Website/{{Reddit}} [[https://i.redd.it/regjs0c3fou71.png meme]] about [[Webcomic/VirginVsChad "The Virgin TwinBee vs. The Chad Fantasy Zone"]].
* [[https://www.youtube.com/watch?v=cc6K6XUGeYw Here]] you'll see Madoka sing a "version" of the oft-remixed song "Gift of the Wind" by Creator/MarikoKouda as well. It's quite the song cover, isn't it? It sounds just like the original![[note]]Madoka's [[DoItYourselfThemeTune voice actress]] is Mariko Kouda.[[/note]]