* Optimus Prime asks Sari how humans make "these new, smaller organics" (i.e. babies). [[http://www.youtube.com/watch?v=zwOdo6uoeK4 His reaction to what he hears is priceless.]]
* "I am Wreck-Gar! I ''dare'' to be stupid!"
* Newly immortal Starscream's [[http://www.youtube.com/watch?v=Zo7-02kedBY death montage.]]
* Just about any scene with Blitzwing and Lugnut in it, but especially their first episode.
--> '''Blitzwing:''' "You stepped in something!" *faceswap* "But with a few dancing lessons, you'll soon be as light on your servos as me, cha, cha, cha!"
** There was a scene ''before'' that as well. [[http://www.youtube.com/watch?v=5dNJrP2gBSI Never has "The Itsy Bitsy Spider" been this funny.]]
** "I will pound you into sheet metal!" *faceswap* "Wait, we've already got some!"
** Don't forget Lugnut's unique devotion. It even annoys Megatron himself:
---> '''Lugnut:''' "I WILL NOT HIDE! I WILL STAND PROUDLY AND SHOUT MEGATRON'S NAME TO THE HEAVENS!"
---> '''Megatron:''' "[[YouHaveGotToBeKiddingMe Oh, for spark's sake]]..."
* "Ohhh, you're scared of me! BOOGABOOGA!" "AHH!" The best part is probably Jazz's terrified pose.
* "You interrupted my SPEEEEEEEEEEEEECH!"
* The entirety of "Return of the Headmaster"'s [[BreakTheHaughty Sentinel-schadenfreude]].
--->'''Sentinel''' [as Optimus fights the Headmaster, who has stolen Sentinel's body]: You're enjoying this, aren't you, Optimus?
--->'''Optimus''': [smirks while continuing to pound the Headmaster, which Sentinel can feel]
** In the ''Allspark Almanac'' databook, a self-serving diary entry from Sentinel Prime makes this even better. "Let me say that again. Optimus. [[PunctuatedForEmphasis Ripped. My. Arm. Off.]]"
** From earlier in that episode, Optimus's reaction to seeing Sentinel Prime as just a head is gold. Hearing [[NotSoStoic Optimus Prime of all bots]] laugh is just a great moment.
*** From the episode:
--> '''Sentinel''': Uhhh, Optimus. Tell the organic Fanzone that he can send his police force home. [[BlatantLies It's a false alarm.]]
--> '''Optimus''': How do you know?
--> '''Sentinel''': ''I just know, okay?!'' Now I need you to come to my position. ''[[PunctuatedForEmphasis ALONE.]]'' And promise me one more thing...
--> '''Optimus''': What?
--> '''Sentinel''': That you ''won't laugh!''
--> ''*Optimus looks around the rubble for Sentinel*''
--> '''Optimus''': Sentinel? Where ''are'' you?
--> '''Sentinel''' (quietly): Look down...
--> *Optimus looks down to see Sentinel's head severed from his body, a [[{{Main/Sting}} comedic cue plays]] as the camera pans down to the body-less Sentinel. Optimus then lets out [[https://www.youtube.com/watch?v=er5E2zZO_MU a hearty laugh]]*
--> '''Sentinel''': ''You promised you wouldn't laugh!''
** Also, "You kicked me! On ''purpose''!"
* At the end of the Season 2 finale, [[spoiler: Megatron in stasis cuffs and Starscream's detached head are stuck somewhere in space, unable to do anything but bicker:]]
--->[[spoiler: '''Megatron''': Stop staring at me.]]
--->[[spoiler: '''Starscream''': No. You stop staring at me!]]
--->[[spoiler: '''Megatron''': Do you think I actually want to spend the rest of eternity gazing at your unsightly visage?]]
--->[[spoiler: '''Starscream''': Trust me: your visage is no picnic either. And your face is ugly too!]]
--->[[spoiler: '''Megatron''': Oh, someone just take me offline now...]]
--->[[spoiler: '''Starscream''': What do you think I've been trying to do for the last four million stellar cycles?!?! AHHAHAHAHAHAHAHAHA! It's funny, really, when you think about it. Tragic, but funny! AHAHAHAHAHA!]]
--->[[spoiler: '''Megatron''': ...oh, shut up. ]]
* This:
-->'''Starscream''': [[TemptingFate This day couldn't possibly get worse]]!
-->[Megatron and Blitzwing appear before him]
-->'''Starscream''': I stand corrected.
-->'''Megatron''': [[SayMyName Starscream!]]
-->'''Starscream''': Sorry, you must have me confused with some other Harrier jet!
** And then a little later from the same episode (especially considering that Optimus and Co have been spending several episodes [[CassandraTruth trying to convince the Elite Guard that there are Decepticons on Earth]]):
--->'''Sentinel:''' *''Sees a crashed Starscream''* What is that thing?!
--->'''Optimus:''' Huh, that's right, you've never seen one up close. It's called a "''Decepticon''".
* Megatron and Starscream's battle of wills continues in the Season 3 premiere "[=TransWarped=]", where Starscream gets so fed up that he starts ranting about how Megatron got them into this mess: "Are you seeing a pattern here? THIS! IS! ALL! YOUR! FAUUUULLLLTTTTT!"
* The 2008 Botcon script reading "Bee in the City" is the funniest thing this troper has ever seen/heard, full of shout-outs and fourth wall breaks aplenty. [[http://www.youtube.com/watch?v=Z4rUveSq7vg Watch]] [[http://www.youtube.com/watch?v=lRLwZZV1YJ4 it]] [[http://www.youtube.com/watch?v=sww0sue3xTs here]].
-->'''Bumblebee''': [[ActorAllusion Maybe we could call Grimlock, or con Lugnut into helping us out!]]
-->'''Joe ([[spoiler:[[WesternAnimation/BeastWars Megatron]]]])''': Please...who do I look like, Creator/ScottMcNeil?
** The entire segment where Shockwave explains what happens when they face a cosmic-powered supervillain, including what the secondary characters do and Optimus Prime's reaction to it.
--->'''Optimus Prime:''' Nnnnnno, we won't be doing that.
*** [[WebVideo/InternetPersonalityVangelus Vangelus']] entire performance as the "morally ambiguous" Transtech Shockwave is brilliant.
* Megatron giving Lugnut orders in "Garbage In, Garbage Out". Seeing how this is Lugnut....
--> '''Lugnut:''' STASIS LOCK ITSELF COULD NOT DETER ME FROM YOUR GRAND, AND ''GLORIOUS'' PLAN, OH WISE AND NOBLE MEGATRON!
--> '''Megatron:''' [[TwitchyEye Just...go....]]
** And earlier, Sari trying to teach Ratchet some manners:
--->'''Ratchet:''' Will you get in already!?
--->'''Sari:''' Ah, ah, ah, what's the magic word...
--->'''Ratchet:''' ''NOW!''
** During the final confrontation with Wreck-Gar:
--->'''Ratchet:''' You glitch-head! You're gonna destroy the whole city! You wouldn't dare do something ''that'' stupid!\\
'''Wreck-Gar:''' I am Wreck-Gar! [[ActorAllusion I dare to be stupid!]] I will destroy the whole city!\\
'''Ratchet:''' All right, you've forced me to make the ultimate sacrifice; to do the one thing that requires more courage than anything I've ever had to do in my entire Autobot career! I... apologize.\\
'''Wreck-Gar:''' Come again?
* After Ratchet saves Bumblebee.
--> '''Prowl:''' I'll stay with Bumblebee.
--> '''Ratchet:''' Try to keep him quiet.
--> '''Prowl:''' You do realize this is Bumblebee.
--> '''Ratchet:''' Oh yeah.
* [[http://www.youtube.com/watch?v=VJIb7uJ0fuY Optimus Prime, behind you!]]
* Prowl's attempt at FakeStatic at the start of "A Fistful of Energon." Creator/JeffBennett just nails it in making it so awkwardly out-of-place for [[StraightMan Prowl]], it's {{adorkable}}.
--> '''Ratchet''': Personally, I've always found Bumblebee's fake static to be a lot more convincing.
* In the first episode, Prowl's reaction to Optimus' 'cog-in-the-Autobot-machine' speech.
-->'''Prowl:''' *''FacePalm''* Not ''this'' speech again...
* The rivalry between [[TheBrute Lugnut]] and [[EvilGenius Shockwave]] during "Endgame". Special mention goes to near the start of Part 1, when after some jibing from Starscream, Lugnut moves in front of Megatron ''punting Shockwave offscreen in the progess''.
-->'''Starscream:''' Y'see, now [Megatron] even likes ''[[MoodSwinger Blitzwing]]'' better than you. Hmm... *''Shockwave meanwhile has walked back onscreen''*
-->'''Lugnut:''' *''Slaps Shockwave back offscreen''* ''NO!'' I am the most faithful!
* In "Megatron Rising Pt. 2," Blitzwing is facing off the Autobots and has the upper hand. Prowl is GenreSavvy enough to have Bumblebee employ his greatest weapon ([[MotorMouth his obnoxious personality]]) and continually [[IShallTauntYou talk]] [[YouFightLikeACow smack]] to Blitzwing, who is strafing the Autobots in his jet fighter mode. This upsets Blitzwing, and causes him to switch to his [[FunnySchizophrenia Angry]] 'Hothead' persona and attack them with his even ''more'' heavily armed tank mode. Blitzwing has PersonalityPowers, but is also an InvoluntaryShapeshifter, and his transformations happen about as quickly as his personality changes. It's only when he [[OhCrap realizes that he hadn't landed first]] that he learns GravityIsAHarshMistress.
* Ratchet's mouth hangng open when Sari repairs Prowl in the second episode.
* ''All'' of Blitzwing in "Human Error". Even a horrible injury wont faze him for long.
-->'''Blitzwing:''' Oh, look! I have a hole in my rocket!"