* When The Doctor *ahem* "re-modulates his vocal cords" with Fluttershy to activate the Hervoken Charm Cube. Try to imagine Creator/DavidTennant speaking in Andrea Libman's "Fluttershy" voice without laughing.
* Pinkie Pie's ability to dumbfound the Doctor is both hilariously in-character and believable.
* The Doctor's reaction to his ponification:
-->"I've heard about regenerations going wrong before, but never one that ended in...pony!"
* Pinkie Pie ''"advertising"'' the summoning charm for the S'Muz, which caused all the ponies that heard it to completely ''crack up'':
-->"Warning: use of summoning charm can cause vertigo, nausea, and may unleash a malevolent cosmic monstrosity upon all reality; do not use under any circumstances," Pinkie Pie said in her best advertising voice.
* Rarity's reaction to the TARDIS.
-->'''Rarity''': It's so... ''tacky!''
-->'''The Doctor''': What?!
* Twilight's computer is programmed to show a virtual [[CloudCuckoolander Pinkie Pie]] whenever it comes up with something that doesn't make sense. Think about that.
-->'''Virtual Pinkie''': Nonsense is what sense sees when it looks in the mirror!
-->'''Twilight''': Why did I even get this app?