* An early one is the utterly ''indignant'' reaction when a farmer mistakes the unicorn for a common horse.
-->'''Farmer:''' I'll curry you up, clean you off, you'll be the prettiest old mare anywhere!\\
'''Unicorn (to herself):''' ''Mare?'' '''''MARE?!''''' I, a ''horse?'' Is that what you take me for?!
* SCHMENDRICK THE MAGICIAN!!... the incompetent but sweet-natured magician.
** "My dear, you deserve the services of a great wizard, but I'm afraid you'll have to be glad of the aid of a second-rate pickpocket."
** "And be wary of wousing a wizard's wath! Rousing a rizard's... Be wary of making a magician angry!"
** "He had me juggling teacups all night long. Teacups! With tea in them!"
* The....... [[BigLippedAlligatorMoment boob tree.]]
-->'''Schmendrick:''' Oh God. I'm engaged to a Douglas fir!
** It's even funnier in the book, wherein Schmendrick very calmly explains to the now-sentient tree [[BlatantLies that he's been engaged to the fir since childhood]]. A very "sorry to break the news to you" explanation, hoping to dissuade the tree's love.
* Schmendrick's conversation with the Skull:
-->'''Skull:''' Say please!\\
'''Schmendrick:''' ''(sighs)'' Please.\\
'''Skull:''' ...No.
** And earlier.
---> '''Schmendrick''': Shut up you pretentious kneecap! How would you like a punch in the eye?
** The Skull in general is just plain funny, (well, up until he realizes that Amalthea is a unicorn....) the crown jewel being when Schmendrick tricks him into telling them what they want to know in exchange for an ''empty'' bottle of "wine". The Skull happily accepts this and chugs the non-existent contents, even somehow getting an alcohol flush despite, y'know, being a skeleton.
* "[[AnachronismStew Have a taco.]]"
* When Schmendrick first encounters Captain Cully and his band:
-->'''Schmendrick''': You're Captain Cully, boldest of the bold and freest of the free.\\
'''Cully''': ''That'' I am.\\
'''Molly''': He's guessing, Cully. Gut him, before he does you [[NoodleIncident the way the last one did]].\\
'''Cully''': Ah, that's only Molly Grue's way. She has a good heart. A ''good'' heart.\\
'''Molly''': Off with ya!\\
'''Schmendrick''': And this lady, don't tell me, she must be your faithful and beautiful companion.\\
'''Molly''', ''(leaning casually back against a tree)'': Maybe he does know.
* On a related note, in the book after the unicorn frees Schmendrick from the tree and they go off together, Molly (who had run off chasing after "Robin Hood" and his Merry Men) catches up with them. Her comment, when she appears out of nowhere from behind a tree?" "Surprise; it's Maid Marian."
* And in a final Molly moment, when she is begging Schmendrick to do something to save the unicorn from the Red Bull, she admits the truth (no matter how badly she'd wanted to believe it before): "You called up Robin Hood, and there ''is'' no Robin Hood!" It's the incredulous delivery that makes it.
** Following this, and Schmendrick turning the unicorn into a human woman, he's ''elated'' at wielding so much power once more. Molly is... less than pleased with the results.
--->'''Schmendrick''': The magic chose the shape, not I! I am a bearer! I am a dwelling! I am a messenger!\\
'''Molly''': You are an ''idiot''!
* Mabruk prepares to attack Haggard, and stops because he recognizes the unicorn, cackling hysterically that Haggard "has let his doom in the front door!" The funny part? Lir sighing and promising to write the old wizard a good reference for future job hunting.
* From the DVDCommentary, one of the commenters tells the story about a Canadian ''Last Unicorn'' fanfic he heard about where Haggard winds up in the ''Series/GameOfThrones'' universe after his castle collapses, ends up sitting on the Iron Throne and defeating all of his opponents, but [[ShaggyDogStory he still isn't happy]].