* Two Words: [[http://www.youtube.com/watch?v=R2p6dQFDaQU Disco Kid]]
** There's just something about that cheesy smile taunt he does during Title Defense that's just '''hilarious'''.
** Disco Kid's pre-fight intro in contender mode.
** Knocking him out causes him to spin around the ring, bouncing off the corners and lighting them up. It's hard not to laugh at just the absurdity of it.
** His between rounds animations in Title Defense mode. "Weeeeeee! I am Fab-u-lous!"
** Doc makes fun of Disco Kid's absurdity in Title Defense mode:
--> '''Doc:''' Hey Mac Baby... I didn't wanna spoil the surprise, but... I bought you a matching leotard for your birthday! Ha ha ha!
* During intermissions while you're fighting Great Tiger, he may [[http://youtu.be/T2gpWisvzi8?t=2m35s change places with Doc Louis]]. Doc's reactions are priceless.
* In the Wii version, you get to knock the ''croissants'' outta Glass Joe. Now say that out loud with the worst French accent you can.
* "[[MemeticMutation After this fight is over]], [[{{YTMND}} I'm gonna go for a ride]] [[HeroStoleMyBike on your bike]]..."
* Super Macho Man's Title Defense intro. He tries to move into the spotlight, which keeps moving away from him. "Why don't you love me?!"
* Go ahead, [[SchmuckBait knock the chocolate out of Doc Louis' hands...]]
--> '''Doc Louis:''' [[BerserkButton Now you done it!]] [[YouWouldntLikeMeWhenImAngry You won't like Doc when he's angry!]]
--> ''(tears off his shirt, [[{{Squick}} creeping out Little Mac]])''
* In the NES game, the only attempt at humor any of the characters make that actually is funny is when Bald Bull says, "My barber didn't know when to quit; do you?"
** From the same game there's also Soda Popinski's "I drink to prepare for a fight. Tonight I am very prepared!"
* At one point between rounds on the Wii game, instead of his usual advice, Doc tells Mac to remind him to pick up some milk after the fight. It just comes outta nowhere, making it hard not to at least chuckle at it.
** "Join the Nintendo Fun-- I mean, Club Nintendo today, Mac!"
** The original Nintendo Fun Club line was pretty funny and out of nowhere as well.
** "What's your favorite flower son? Mine's chocolate!"
** More of Doc's great advice: "There are many ways to eat a chocolate, but there is only one way to eat a chocolate bar!"
* Decking Narcis Prince in the face can be quite amusing.
* Super Macho Man tends to get a lot of funny moments, but the best is how [[AttentionWhore even though he constantly plays to the crowd]], they always boo him. Goes UpToEleven when he wins in Title Defense
-->'''Super Macho Man:''' *With the crowd still booing him* Oh, now you love me. Now you love Macho Man. Well, it's too late...Maybe not!
* Some of the pre-fight cutscenes are worth a good chuckle. [[http://www.youtube.com/watch?v=eiDsWK5a4Ck Title Defense Bald Bull]] will punch ''himself'' out for a few seconds, [[{{Beat}} only to pause briefly as if considering what he's doing]], then decide punch himself once more before getting up for the real fight.
* Don Flamenco's ridiculously over-the-top defeat animation in the Wii version. He holds up a rose similar to old cartoon characters when they pretended to "die."
** Also from the Wii version: Don's anger when you knock off his toupee. The fact that the match had to momentarily stop just so he could react to it is also hilarious.
* Mr. Sandman's Title Defense victory cutscene, which has the referee trying to raise his arm in victory, but can't even budge it, and Sandman then lifts his arm into the air with the referee still holding onto it.
* Aran Ryan is a walking funny moment in the Wii version. Special mention should go to his fight loading screen, in which he runs around like a lunatic and yells in Mac's ear...without Mac even flinching.
** "YA DON'T LOOK IRISH!"
** "YA PROB'LY GOT CHEESEBURGERS IN THOSE GLOVES, HAVE YA MAC?"
* Bear Hugger in the Wii version bringing in a ''squirrel'' to help him box. Every time Bear Hugger gets knocked down, the squirrel comes out and jumps on his stomach to try to get him to wake up.
* Doc can't help but fool around when Mac's fighting [[spoiler: DonkeyKong.]]
--> ''Doc: Listen up, son. If you're having trouble...maybe I should call [[SuperMarioBros my plumber!]] Ha ha ha!"
--> '''Doc: Ok Mac, here's the deal... You go and distract this gorilla! Then I'll go and steal his bananas and make myself a chocolate sundae! Ha ha ha!