!!!Main series:
* Doakes' face when he [[http://www.nuzlocke.com/pokemonhardmode.php?p=22 kept spamming Magnitude.]]
* Ruby, a man of priorities, gives us this exchange as Gulliver the Pelipper is flying him over Mossdeep while they're chasing Team Magma:
-->'''Ruby:''' Hey is that a town?
-->'''Gulliver:''' But what about Team Mag-
* [[http://www.nuzlocke.com/pokemonhardmode.php?p=9 "Who's there? Show yourself!"]]
* '''''Goddamnit Ralph!'''''
* Sawyer's self esteem HARSHLY FELL.
* "I don't understand... how could ''water'' be ''good''?"
* [[http://www.nuzlocke.com/pokemonhardmode.php?p=30 The Warden getting his teeth back.]]
* [[http://www.nuzlocke.com/pokemonhardmode.php?p=18 Pretty much this entire comic.]]
* [[http://www.nuzlocke.com/pokemonhardmode.php?p=42 This exchange]]:
-->'''Announcer:''' Let's see what you folks down in the stands think about our upstar little challenger...\\
'''Mr. Fuji:''' I think he's cool. Much Poke-respect.\\
'''Erika:''' He's pretty chill. Y'know, for a square.\\
'''Bill:''' I think...\\
'''Lt. Surge:''' '''-I THINK HE SUCKS AND HE OWES ME A [[PrecisionFStrike FUCKING]] GYM!'''
* A good MoodWhiplash moment. During Ruby's final battle with Gary, after Bruce fails to finish off Blastoise with a Blast Burn and must recharge, Blastoise grabs Bruce, fires off a Hydro Pump point-blank, [[EpicFail and misses]].
* Some moments with Ruby's conversation with Giovanni in the finale such as showing him the Kabuto, and this exchange:
--> '''Giovanni:''' Fortunately you still have the Masterball so you can end all of thi-\\
''[Ruby breaks the Masterball]''\\
'''Giovanni:''' ''[{{Beat}}]'' WHAT THE [[PrecisionFStrike FUCK]]\\
'''Ruby:''' Sorry, Giovanni... but rules are rules. ''[Flies away on Bruce]'\\
'''Giovanni:''' [[CallBack WHAT RULES]]?!
* Giovanni and Maxie in prison. Fucking priceless.
-->'''Maxie:''' So... How do you feel about water?
* When Ruby finds [[spoiler: Hilbert's]] body in the closet his response of "Arceus Christ" is just hilarious.
* Ruby's reaction to being [[spoiler:forced to pick Oshawott]]. The expressions on [[spoiler:Oshawott]]'s face make it even better.
* Ghetsis has only just shown up and his speech is golden. "Allow me to reveal a simple truth. Pokemon fucking hate you. Seriously, you capture and pit them against each other their will. Would they fight back if they wanted to be caught?"
** And the cop telling them about orders to "liberate" the park. After a complaint about freedom of speech, "Oh yeah? Well I've got freedom of repel." ''[cue using the pepper spray to get them out]''
* [[http://www.nuzlocke.com/pokemonhardmode.php?p=58 Meeting N:]]
-->'''N:''' Your Pokémon... it's talking.\\
'''Ruby:''' No he's not.\\
'''Luke:''' [[BlatantLies No I'm not.]]
* Ruby sees [[HotScientist Professor Juniper]] for the first time:
-->''[[StatOVision MILF ALERT!! ENGAGE IN 3... 2... 1...]]''
-->'''Ruby:''' Wait a minute...
-->''[Ruby sees Bianca peeking out from behind Juniper]''
-->''[[StatOVision ABORT!! ABORT]]!!''
-->'''Ruby:''' ''[while running away]'' FUCK FUCK FUCK
* [[http://www.nuzlocke.com/pokemonhardmode.php?p=82 The first course]].
** Ruby's Pansear VS. Cilan's Pansage.
* Ruby stealing, Cheren and Bianca's gift, and his plans for [[ButtMonkey Senor Fluf]] once he finds what's inside.
--> '''Bianca:''' [[http://www.nuzlocke.com/pokemonhardmode.php?p=60 FUCKING MONKEY]]!!
** "Yeah, back in college me and Juniper did [[DoubleEntendre "experiments" together]] all the time..."
* Fennel's brief flashback of her time at Castelia University with Juniper after Bianca asks how she knows the detailed effects of Dream Mist. The strip makes the use of Dream Mist out like a bad LSD trip.
-->'''Fennel:''' ...I read it in a book. ''[Ruby knows she is lying]''
** What made it more amusing was Minccino's expression in the flashback with Fennel and Juniper can be seen as funny given the two were on a MushroomSamba.
-->'''Nuzlocke:''' Don't do drugs. You'll make Minccino sad.
** To add to that, the Plasma Grunts seeing Satan just chilling behind Ghetsis is pretty funny.
* Ruby and Cheren decide to team up. Cheren takes things to far and ends up ''ripping a heart out of a grunt's chest.'' Grunt's response?
--> '''Grunt:''' What the hell, man...
* This moment in the museum:
--> '''Burgh:''' Castelia...? ...That's MY turf!
--> '''Ruby:''' And who are you suppose to be?
--> '''Burgh:''' I'm a friggin' gym leader, who the hell are you?
--> '''Ruby:''' Wow. A gym leader not being in their gym and actually doing their goddamn job. There's a surprise.
* Ruby and a Petilil have a [[{{Kawaii}} kawaii showdown]].
** And the Plasma Grunt's subsequent reaction of pure horror.
** It's even funnier now that there is new merchadise on the site shop showing them in "Kawaii-Mode".
* Ruby's reaction to Bianca asking Iris to be her bodyguard despite being younger than she is, and seemingly implying that she chose her because she was [[ScaryBlackMan Black]]. His reaction is then echoed by Ghetsis in the following scene.
--> '''Ruby''': I can't tell if you're being racist or just retarded right now.
* Ghetsis and Bronius discussing people from Johto and their [[ScrappyMechanic calling habits]].
** "There is nothing wrong with people from Hoenn" in the same panel with Ruby about to break into a window on a vehicle.
* The entire arc where Ruby catches [[spoiler: Victini]]. First, when Ruby finds him, the [[spoiler: Victini]], in the dark, makes a BadassBoast about itself, only to be interrupted by them turning on the lights, revealing he lives in a modest apartment. Which soon Ruby mocks him for only being lvl. 15, while Pauly accidentally views [[spoiler: Victini's]] blog, hitting his BerserkButton. Which leads to Ruby pelting him with a mountain of Pokeballs before he gives up. Ruby also gets some nice revenge on Burgh shortly afterwards.
* Pretty much every thing about Episode 9.1. Here are some notable examples:
** The famous scene with Bianca and her father in Nimbasa City was TearJerker in the games; in this comic, it becomes a SoapOpera parody.
** Vincent's less than amused face when Ruby enters him into an elegant-themed musical. Bonus points when Ruby off-handedly mentions that he intended to enter Princess, but selected him instead. As for the play?
--> '''"It. Was. Elegant!" -Nimbasa City Times''

!!!Robotv7's Nuzlocke Challenge ([=FireRed=] and Sapphire):
* From Robot7's comic there's his attempt to make his Hitmonlee more unique.
--> Let's see what [=TMs=] we have... *finds Attract* oh god yes...
* Also from Robot:
-->'''Robot:''' Man, i should switch Pchooooo out... (Beat) Naw, you can do it Pchooooo!\\
'''Pchooooo:''' ''[sweat]''\\
'''Robot:''' We need a critical hit.\\
''Not a critical hit.''\\
'''Robot:''' ''[gasp]''\\
'''Pchooooo:''' ''[tears]''\\
''[[PsychoticSmirk Machop used..]] [[spoiler:[[EpicFail Focus Energy!]]]]''
* Robot altering Dewford's trendy phrase.
-->'''Kid:''' Are you sure...?\\
'''Robot:''' Positive.\\
'''Kid:''' OK! Hey everyone! The new trendy thing to say is [[spoiler:'''''ADULT TOYS!''''']] ''(Robot runs off laughing)''

!!!Freddy's Nuzlocke Adventure:
* Rosie's [[http://imgnook.com/hZrER reaction]] to her first real battle being against an Onix. That face is ''priceless''.
-->'''Rosie:''' [[WhatTheHellHero what the fuck man]]
* Freddy encounters his rival in Pokemon Tower, with some Nuzlocke-flavored Fridge Logic adding a touch of hilarity.
-->'''Gary:''' Your Pokemon aren't dead. Why're you here? Eh, no matter. I can at least make 'em FAINT.\\
'''Freddy:''' (pause as it hits him) '''''YOU SON OF A BITCH'''''
* Lance's first apperance:
-->'''Lance:''' Are you a helpless 10 year old child?\\
'''Freddy:''' Yes.\\
** Later, in Notepad's [[ExactlyWhatItSaysOnTheTin no-sleep run of Crystal]], despite answering no, Lance ''still'' says exactly the same thing.
* "[[http://imgnook.com/4jS2M And so]] Daisy drank... and drank... all the way to the Pokemon Center."
* The TrainingMontage before the Elite Four match in his Gold run. Also a CrowningMomentOfAwesome.
* Freddy battling Sabrina, [[spoiler: [[Series/SabrinaTheTeenageWitch the Teenage Witch.]]]]
* [[http://img818.imageshack.us/img818/3619/fredwhite8.png I DON'T GIVE A FUCK]]
* [[http://img40.imageshack.us/img40/5511/fred38.png I have two water pokemon!]]

!!!Petty's Nuzlocke Runs:
* Rick at one point learns Rollout. [[RickRoll Guess which song he sings for an entire battle later on?]]
** I believe it was verified somewhere that this was the entire point of his nickname.
** Of course, same battle also has a harsh case of MoodWhiplash when [[spoiler: Leroy the Flareon dies due to an Electrode's self-destruct]], with the music's lyrics playing in sync. Aside from that, the song makes it equal parts funny and awesome.
* [[http://pettynuzlockehg.smackjeeves.com/comics/1463764/part-6/ And then I'll use that money... To buy WEED!]]
** Which is an absolutely brilliant ShoutOut to the Creator/TeamStarKid musical ''Theatre/{{Starship}}''.
* When Locke sees a Nidoran in HG, a sailor moon ShoutOut (complete in a art-style parody) [[BigLippedAlligatorMoment occurs out of nowhere.]] What then follows is a FlatWhat after it's done.

!!!Nonparael's Nuzlocke Runs:
* Ara's Black run introduces quite possibly the best Alder ever: "Do you know who I am? I'm the goddamn Champion. '''FIGHT. NOW.'''"
* Ara discussing with N how to [[http://nonparael.deviantart.com/art/Black-Nuzlocke-26-203548133 "improve"]] the Team Plasma uniform... and [[HardGay what they come up with]].
** "Wait a minute. Something has gone horribly wrong." "Let's never talk about this again."
* [[EvenTheGuysWantHim Ara]] reassuring Cheren of his sexuality... by way of [[MarshmallowHell Bianca's boobs]]. HilarityEnsues.
** [[http://fc06.deviantart.net/fs71/f/2011/149/b/a/black_nuzlocke___41_by_nonparael-d3hjt2v.jpg Here's a link.]]
* [[http://www.deviantart.com/download/202141005/black_nuzlocke___23_by_nonparael-d3cckzx.jpg Eeeeeeeeeeeeeeeeeeeeeeeee]]
* From Ara's Pearl run, we have [[http://fav.me/d2zzqt2 Ara and Cyrus]] [[YouFightLikeACow insulting each other]] through their BossSubtitles.
-->'''Cyrus's Subtitle:''' Galactic Leader seeks [[FanNickname Space]] [[DoubleEntendre Penis]]\\
'''Ara's Subtitle:''' Speaking of which, [[GenderBender how's the space where YOUR penis used to be?]]
* His Soul Silver [[LuckBasedMission "Game Of Chance"]] Nuzlocke is chock full of these. Everything from Edge's behavior to scribbling out Silver's name and writing in "Sugartits" before giving it to the Police Officer.
* Ara learned the hard way that [[http://nonparael.deviantart.com/art/Black-Nuzlocke-38-209053123 hugging a Pawniard]] [[OverdrawnAtTheBloodBank is not the best idea]].
* While in the middle of raiding Team Galactic's Eterna Headquarters, Ara [[http://nonparael.deviantart.com/art/Pearl-Nuzlocke-06-178896654 accidentally stumbles on a group of grunts playing Strip Poker]].

!!! Earl Grey's Ginjika Nuzlocke:
* Diego "Cook" the Quilava [[http://earlgreyxx.deviantart.com/art/PGNC-7-Part-2-278182934 posting in his tumblr about how much he hates Azalea Town]], and then stumbling with a local ScaryBlackMan Ekans, whom he believes has angered about the trash-talking he did about the town. After fighting him, [[http://earlgreyxx.deviantart.com/art/PGNC-8-Part-1-279751108 the Ekans confesses he doesn't like the town either]].
* Cook decides that his team needs a water Pokemon, so when he sees a lone Wooper he sends Audrey the Hoothoot to hypnotize him until K.O., then he and Belmont (the aftermentioned Ekans) carry him away and convince him to join the team.
--->'''Belmont:''' Cook, this isn't going to look suspicious? I mean, [[UnfortunateImplications a black man and a Mexican carrying out a fancy looking white boy-]]
--->'''Cook:''' Nigga, [[LampshadeHanging this is Pokemon, shit like this happens all the time!]]
* Marcel the Wooper is initially silent upon waking (aside from punching out Cook for kidnapping him). When he finally speaks, it's with a [[BadassBaritone deep, accented voice]], justified by his smoking habit but still [[VocalDissonance distinctly unfitting for a fourteen-year-old]] (or a [[RidiculouslyCuteCritter Wooper]]).
--->'''Cook:''' *laughing* That gringo sounds like [[LooneyTunes Pepe Le Pew!]]
--->'''Marcel:''' [[DeadpanSnarker I am beginning to regret this decision already.]]
* [[http://earl-greyxx.deviantart.com/art/PGNC-11-Part-2-317159884 In the middle of this update,]] in between Diego training with and taking pride in his new teammates and a dramatic glimpse into his backstory, is a scenic view of his old home with a beautiful night sky... and [[MoodWhiplash the stars are spelling out "DICKS"]].

!!!Manic's Screenshot Runs:
* In Fatal Platinum, how does our protagonist keep Lucian out of his mind? By conjuring up mental images of the most grotesque, hilariously vile things he can think of. The only thing specifically noted is "Pichu/Miltank pairings," but... well. And then when Lucian tries to read his Medicham (who has been a serious character throughout the run):
-->'''Lucian:''' Brrrr...let's see what you have in YOUR mind instead. A fellow psychic would never-
-->'''Diell:''' Hippowdon lapdance.
-->'''Lucian:''' OH COME ON!
* Manic tearing down the Voltorb Flip mini-game in Spirit Electrum to get thousands and thousands of coins, and the team's proceeding reactions.
-->''*Manic advances to Lv. 7 of Voltorb Flip*''
-->'''Link:''' ''WHAT THE FUCK.''
-->'''Manic:''' What? It's easy.
-->'''Will:''' The old guy is staring at you like you've got a tree growing out of your head!
-->'''Gail:''' It's only a matter of time until you make a ''fatal'' mistake!
-->'''Link:''' Ha! Gail's right. I mean, sure, you got this far, very nice. But this is the ''maximum'' level. There's no way you can-
-->''*Manic clears Lv. 7 as well*''
-->'''Link:''' What ''are'' you?!
* During Blinding Light, Mccoy the Escavalier sweeps Marshal while doing a hoedown. Which is funny in itself, but this tidbit stands out.
--> '''Zerzan:''' I can't believe this is working.
--> '''X-4:''' He'd probably stop if Vile told him to. Vile?
--> '''Vile:''' Yee Haw.
--> '''X-4:'''Well, okay then.

!!!Kynim's Runs
* Ky-nim's Diamond run was full of funny moments, most of which involved either [[{{Keet}} Hai]][[CampGay si]] or Team [[LadyGaga Gaga]].
** "Behold... [[PhysicalGod DIALGAGA]]."
* [[http://ky-nim.deviantart.com/art/Nuzlocke-White-003-208803217 Seeing N in Accumula:]]
-->'''N:''' [[ComicBook/TheCreeper Hi...y-your Pokemon...did...did you know...he talked...and...you're pretty.]]
** [[http://ky-nim.deviantart.com/art/Nuzlocke-White-004-209404725 The fight that ensued after that meeting]]
* Any scene with [[CampGay Burgh]] and his [[{{EverythingsBetterWithRainbows}} Rainbow Aura]]
-->'''Burgh:''' Grab my scarf!
* [[http://ky-nim.deviantart.com/art/Nuzlocke-White-020-286825549 The N Ferriswheel scene]], which most Pokemon fans chalk up as romantic but the scene is anything but that.
* In the middle of Part 38 of her White Run, Alder is hosting a conference among the Gym Leaders. Good luck trying to take it seriously since [[CampGay a certain someone's]] [[BattleAura rainbow-colored aura]] [[http://www.deviantart.com/art/Nuzlocke-White-038-427936451 is hard to ignore]]

* From Dennis's Revenge: The Johto Takeover:
-->'''Falkner:''' Dennis, why do you seek to fix what Arceus ([[spoiler:who is masquerading as Silver]]) has done?\\
'''Dennis ([[spoiler:actually Genosect in human form]]):''' Look at this place! Legendaries all over, level 3 fully evolved Pokemon, even Pokemon that don't belong in their regions are here! And you still have your two derps!\\
'''Falkner:''' You did NOT just call my precious birds [[InherentlyFunnyWords DERPS]]! I'll take you down! Go, Pidgey!
* In Lenni's white run, [[http://fc00.deviantart.net/fs71/f/2011/099/6/d/a_really_stupid_comic_part_2_by_thewickedkid-d3dmtne.jpg when Nel's sewaddle evolves.]]
* Azer's run has at least one in every page... but a combination of the earlier image of Godzilla (a charmeleon) hugging Azer and the later statement "He likes hugs" takes the cake for this troper.
* In Aouli's run, Canterbury the Butterfree explains to the protagonist what "flashing" is. And no, not the HM move.
* Pencil's Leaf Green Nuzlocke [[http://yoroshii.deviantart.com/art/Pencil-Nuzlocke-pg6-204571692 Drunk Dallas makes everything better]]
* Azza's [=LeafGreen=] run at the beginning of [[http://www.majhost.com/gallery/thingi0ma0jig/Nuzlocke-LeafGreen/01azza08.jpg comic 8]], when Jasmine the Jigglypuff joins the party:
-->'''Jasmine:''' (using Sing) [[{{Kesha}} I wake up in the morning feeling like P-Diddy...]]\\
'''Azza: ''NO.''''' [[TakeThat Learn real music.]] (puts a pair of headphones on Jasmine)\\
'''Jasmine:''' What the... (music starts blasting, Jasmine becomes stunned)
* In Oh Gosh, Platinum Nuzlocke, naming a psyduck "Implying I."
-->Implying I used water gun. Implying I grew to level 18. Implying I can't learn more than four moves. Implying I forgot how to use tail whip. Implying I learned confusion!
-->'''Oh Gosh:''' That was amusing. *boxes Implying I*
* [[http://land-walker.deviantart.com/gallery/#/d4b3t14 Why its probably okay to have the No Dupes rule on, after getting 5 of the same damn thing, you are like a derp magnet.]] And yes, there is a facepalm.
** [[http://land-walker.deviantart.com/art/Yellow-Nuzlocke-part-9-262735383 The following comic]] provides possibly the ''best ever'' interpretation of a Diglett's body underneath the soil. It must be seen for itself, to describe it would be to spoil it.
* Urahara's Scorched Earth Nuzlocke:
-->(after nicknaming a Skiploom Deedeelion)\\
'''Verona the Mismagius:''' [[SarcasmMode You're the most creative person.]]\\
'''Jasmine:''' Shut up. Or do you want me to rename you oldladyhat?
* In Infecting the World: The Ultimate Bug Monolocke, ZP's opinion on Victory Road's boulder puzzles:
-->"It's like there are magical fuck-you gnomes that want to make your day miserable."
* Golden Bells has a very funny prologue. Starts off serious, with a text about the challenge, all while [[http://fc09.deviantart.net/fs70/f/2012/221/4/2/gb__prologue_by_inkypaws_productions-d5ai5k0.png the protagonist is being dragged by her starter.]]
* French's FireRed nuzlocke features [[http://i14.photobucket.com/albums/a317/french-teapot/Page14.jpg Wilton the Gloom. Talking his way through the Cerulean Gym]]
* [[http://cheezburger.com/6796993024 This guy]] makes the best of a bad situation.