* [[http://www.youtube.com/watch?v=Vev301Brlfg "Here Sakamoto, Have Some Gum."]]
* [[http://www.mangafox.com/manga/nichijou/v01/c009/2.html Manga chapter 9]], a dialogueless attempt by Mio and Yuuko to build a house of cards that predictably ends in disaster. Mio weeps as if someone died.
** Anime episode 17 adds orchestral music and Nano threatening to push over the cards with sleepy nose bubble.
* In episode 1, Nano accidentally runs into a boy while running. This would easily fall under MeetCute... had it not been for the spectacular explosion that resulted from it. Bonus points for the slow-motion shots of Nano's sandal falling to the ground and a petal landing on her head just before the explosion.
* The entire deer vs principal fighting scene. Never before has the fight of Man vs. Deer looked this epic.
* Nano's dilemma with the cockroach under the bowl. The way she processes the situation in her thoughts is so much like your average action anime scene, with all the drama of having to disarm a bomb. It only gets funnier once Sakamoto shows up.
-->'''Sakamoto:''' How am I supposed to get it if you don't lift the bowl?
-->'''Nano:''' Lift it yourself!
-->'''Sakamoto:''' How am I supposed to do that?!
* The camping trip. ALL OF IT. It's basically a conga line of bad stuff happening to the girls, made even worse for them by Mai's [[TheGadfly pranks]].
** For that matter, any time Mai pranks Yuuko is seriously hilarious. Episode 4 is particularly ''epic'' in this respect.
* Sakamoto's first words, and the events leading up to them.
* [[http://www.youtube.com/watch?v=attYgMjCXGc&feature=related This little scene in episode 5]]
* Episode 5's Helvectica Standard: ProjectileToast that launches with enough speed and force to [[spoiler:punch a hole in the roof and kill an unsuspecting mouse.]]
--> '''Mouse:''' "[[GivingUpTheGhost Chuu~]]"
* The death of [[DeathByFallingOver Dolph]].
* Any of Nakamura's failed attempts to kidnap Nano.
* The popcorn scene in Episode 8.
* ''[[TemptingFate Invincible aliens are immune to any and all attacks!]]''
* Any time Yuuko thinks she's doing something right, only for it to backfire immediately, and her off-the-wall reactions to it. Happens OnceAnEpisode at least!
* Episode 9: After surviving a test together, two friends cry tears of joy, another student tells everyone they are getting a free bag of rice, and two students announce they are getting married.
* Episode 10: Annaka goes to a ''[[https://en.wikipedia.org/wiki/Nagashi_somen nagashi somen]]'' event, only to find that it's [[spoiler:a cheap imitation of the real thing.]] Endless "EEEEEEEEEEEEEEEEEEEEEHHHHHHHHHH??" ensues.
* On episode 12, Yukko's face after discovering she doesn't have money in her wallet is just hilarious.
** Also episode 12, Sakurai and the clerk at the coffee shop have a complete communication breakdown. [[ApologizesALot Awkwardness ensues.]]
* Episode 15: The whole sequence where Takasaki-sensei is completely confused and breaks down over Sakurai-sensei's and a student's [[NotWhatItLooksLike relationship.]] [[spoiler: [[RelativeError He was actually her brother]]]]
* Episode 16: Yuuko meets the Professor. Doubles as a CrowningMomentOfHeartwarming because of how cute they and their interactions are.
** Mio goes berserk when her CovertPervert drawings get exposed and in the process of trying to collect them all attacks a cop, Yuuko, Oh Gentleman, and Sasahara's goat.
*** The build-up to that moment is also funny: When Mio realizes that she coincidentally has the same kind of bag as a criminal the cop is after, she gets nervous and keeps acting more and more suspicious. Yuuko's over-the-top reactions are hilarious. At one point, Mio even puts her hands forward as if she was turning herself in, and Yuuko tries to cover for her by saying that she's just preparing in case a stray volleyball comes her way.
* Episode 17: Nakamura-sensei's PitTrap gets Nakanojo instead of the desired Nano. What does she do? Pull out an electric trimmer and shave off his mohawk.
** Mai's trolling gets so out of hand, Yuuko unleashes a SkywardScream so loud that most of the population hears it. This includes the school gym, Sakamoto back at the lab, a mugger holding his victim at gunpoint, said victim, and some dude ''living in the sewers.''
* Episode 18: Yuuko is playing one of those crane games. She has the toy firmly grasped in the claw and proceeds to bring it up... only for the claw to suddenly break off mid-ascension. Her face right afterwards is priceless.
** Nakamura-sensei pours some paralytic powder into a cup of coffee, notices it's gonna overflow, ''[[EpicFail promptly sips the excess coffee]].''
** Yuuko is riding the train to a neighboring town to get to a Daiku Burger restaurant where she was supposed to meet with Mai. However, she keeps embarrassing herself, causing a little girl (A certain {{Tsundere}}'s little sister, to be exact) to keep laughing at her. She eventually takes Yuuko to the DB, where she meets up with Mai, ''dressed in a full medieval armor''. Believing it was Halloween, Yuuko grabs a pair of funny glasses and fake nose from her bag, puts them on, and says "[[GratuitousEnglish Trick or treat]]". Misato then remarks how Halloween ended a week ago, while her sister is laughing so hard she's banging on the table.
** In a random and surprisingly beautiful segment from "Helvetica Standard", a young woman is trying to get to an old library to find a book that she had read in her childhood. She eventually finds that book, and sits down to read it. She opens it... ''[[spoiler: and finds a gun inside]]''.
* Episode 19: Mio attempting to jump over a pole. After a long InnerMonologue you're expecting a SugarWiki/CrowningMomentOfAwesome...instead, she jumps and nails Yuuko in the stomach with a headbutt. Yuuko tackles her as well so she lands on the mat.
** While waiting out a storm in a shrine, Yuuko decides to offer a donation. [[ForWantOfANail Her coin bounces off of Mai's book and is wedged into the ground.]] [[EpicFail She rings the bell and it breaks and hits her head, the floor under her collapses and the bell falls on her head again...]] [[DisasterDominoes then bounces off of Mio, making her slide off of the stairs and then bounces towards Mai who dodges it...and then a shoji lands on her.]] [[DissonantSerenity All while they have the same blank expression on their face.]]
** Here you go : http://www.youtube.com/watch?v=L4GN2Ffix6E
* Episode 20: Yukko's helping Mio with her manga, and grabs a mechanical pencil. She tries to click it... from the wrong end. Her scream explodes through the house, out into space and blasts through all of the planets ([[PlutoIsExpendable And Pluto]]).
* Episode 22: What Nano wants as Christmas presents.
** Yukko and Mio get bitten by Mai's two dogs, causing them to scream so loud that they fire {{Wave Motion Gun}}s [[BreathWeapon from their mouths]] that pierce the heavens along with several buildings.
* Episode 23: Sakurai-sensei finds her brother's porn magazine. HilarityEnsues.
** [[TheGadfly Yoshino]] makes her sister Mio taste some jam she just made. It's ''absolutely nasty'', to say the least, so much so that [[MyLifeFlashedBeforeMyEyes her life flashed before her eyes]] !
* The ''[[http://www.youtube.com/watch?v=MDJ8iWUTKK8 Helvetica Standard]]'' from Episode 24: A girl convinces her friend to tell her the name of the boy she likes, promising one million yen if she reveals the secret. ''Immediately'' after being told, the girl tells the entire class and then plops down a stack of bills on her friend's desk.
* Episode 25: Nakamura-sensei discovers the Shinonome Lab. Her paranoia promptly runs amok.
* On the fan-made side of things, [[http://www.youtube.com/watch?v=NFUjaGotkK0 this video]] features the second opening of the anime set to the theme for ''Series/GameOfThrones.'' In the poster's own words: "This shouldn't work."
* [[http://www.youtube.com/watch?v=zIGCK8rYCVg Mai pretending to be in love with Yuuko]].
* [[http://www.youtube.com/watch?v=r-3ieAwzecI This scene]], where Yuuko and Mio try to break a pumpkin open. The catch is that this pumpkin seems to be made of titanium or something, as it just won't even get a scratch, no matter how hard the girls hit it.