!!From the Series itself:
* "The Ticket Master":
-->'''Twilight:''' Girls! The second ticket is obviously for a date, and I am not gonna take one of you morons.\\
'''Applejack:''' Well, hang on. Y'all can't even get a date.\\
'''Rainbow Dash:''' ''[butting in front of AJ]'' Yeah! And that means that you can take one of us!\\
'''Applejack:''' ''[butting in front of Dash]'' And that means that you can take me!\\
'''Rainbow Dash:''' ''[butting in front of AJ]'' Please, notice me!
* In episode 4, Trixie argues with her audience, and she and Rarity end up going off on a tangent.
-->'''Trixie:''' What do you think ''you'' know about drama?\\
'''Rarity:''' Well, nothing much really, because I wasn't in the drama club when I was in high school. I was far, far, ''far'' too busy getting laid by cute athletic colts.\\
'''Trixie:''' Shut up! I got laid in high school!\\
'''Twilight:''' Well, I sure didn't...\\
'''Rarity:''' It doesn't count if he was in the drama club.\\
'''Trixie:''' I once dated a guy in the marching band.\\
'''Rarity:''' Well, okay, but was he cute?\\
'''Trixie:''' Well, I thought he was cute at the time.\\
'''Rarity:''' Well, fine. I guess that counts.\\
'''Trixie:''' The guy in the drama club was cute too.\\
'''Applejack:''' Gal, are you sure you wanna be on stage for this kind of discussion?\\
'''Trixie:''' Yeah, why?\\
'''Applejack:''' Don't y'all think that that's maybe just a bit personal?\\
'''Trixie:''' No.\\
'''Rainbow Dash:''' Hey! I think the show is still boring!
** Later:
-->'''Rarity:''' I've been thinking, and it sounds as though you've slept with at least two boys...\\
'''Trixie:''' Eh, in high school.\\
'''Rarity:''' And frankly, that's two more than Twilight's ever had, so I guess you win.
* Zecora: "The cure is nudity." Twilight: "But I'm already naked!"
* Rainbow Dash trying to "counter-curse" in Episode 7 and ''Haunting Nightmare'': '''"BLEEEEEEEEEEEEEEEEEEEEHHHHHHHHHH!"'''
** Poor Applebloom.
-->'''Twilight:''' Well, in ''that'' case... let's just run her out of town.
-->'''Applebloom:''' [[TheHeart Run her out of town? Ain't there a diplomatic solution?]]
-->'''Twilight:''' A diplomati---wh?! Whose kid is this?!
** Or the reveal that Fluttershy got mind powers, in one of the best exchanges in the series:
-->'''Twilight:''' I know your stupid name!
-->'''Fluttershy:''' No, you don't, all you thought was "Butterfly."
-->'''Twilight:''' What do you mean all I thou-- ''(beat)'' ... [[PsychicStatic RUBBER DUCKY, YOU'RE THE ONE! YOU MAKE BATHTIME LOTS OF FUN!]]
-->'''Applejack:''' ''([[FunnyBackgroundEvent As Twilight is singing]])'' Can someone please explain to me what just happened? [[MindScrew Because I think I missed a step.]]
-->'''Fluttershy:''' Twilight just figured out that I can read her mind and now she's thinking about a rubber duck.
-->'''Applejack:''' Wait, y'all can read minds? Since when?!
-->'''Fluttershy:''' Since I got cursed.
-->'''Applejack:''' Hang on, so I get tiny and you get special mind powers?
-->'''Rainbow Dash:''' [[SincerityMode Are we gonna form a superteam?]]
-->'''Applejack:''' Fluttershy's the only one with superpowers!
-->'''Rainbow Dash:''' Well, you're super tiny and my wings are super backwards. That's pretty super.
-->'''Fluttershy:''' No, it's not a power, it's a curse. I can't control it, and [[DirtyMindReading now I know that all the guys think really gross things about me!]]
-->'''Rarity:''' That sounds like a superpower to me.
-->'''Fluttershy:''' You were gonna [[WhatTheHellHero invite me to dinner, surprise me with dates, and then guilt trip me into a foursome!]]
-->'''Rainbow Dash:''' [[CovertPervert That's so cool, do me next.]]
-->'''Fluttershy:''' You're thinking of a rubber duck in a tophat.
-->'''Rainbow Dash:''' [[CloudCuckooLander And he's got a monocle. 'Cause he's rich!]]
* [[CallBack "Rubber Ducky, you're THE ONE!]] [[Series/SesameStreet You make bathtime LOTS OF FUN!"]]
* "Can I have a coloring book?"
** Commence the Rainbow Suicide Attack!
* Most of what Sweetie Belle/[[spoiler:Thrackerzod]] says in the final scene of episode 10 is hilarious, due to how badly the apparently normal pony... thing... blends in.
-->'''Sweetie Belle''': I AM BLENDING IN!
* [[CrossesTheLineTwice Nazi!Rarity]]. Heck, Rarity in general.
* Any time Twilight mentions [[HilariouslyAbusiveChildhood her childhood]].
* Twilight explaining herself to Celestia in Episode 8. Even funnier thanks to the return of [[Series/SesameStreet The Rubber Ducky Song]] from Episode 7.
-->'''Twilight:''' Noooo, noo, that was wrong! So wrong!
* Spike's "They threatened me with Tiny Hammers!"
* The shenanigans in Episode 9.
-->'''Twilight:''' But every pony in Ponyville has some kind of ridiculous name like Carwash Rugmuncher or something, and it always turns out that it has something to do with their special talent, like they're some kind of lesbian, carwashing expert.
-->'''Rainbow Dash:''' Hey, do you think you could get Celestia to legally change my name?
** Which is later followed up by...
-->'''Pinkie Pie:''' I left my MP3 Player alone with Gummy, and the last time I did that [[CoolAndUnusualPunishment he deleted all of my music and replaced it with bizarre pornography!]]
-->'''Twilight:''' Your... pet alligator did that?
-->'''Rainbow Dash:''' Naw, it was totally me.
-->'''Pinkie Pie:''' YOU! Oh, the things I did to Gummy are only half of what I will do to you!
-->'''Rainbow Dash:''' [[BadassBoast No-one can touch Lesbian Carwash!]]
* "I think a tiny, evil creature is living in my attic. It likes geometry. And Satan."
* "Call of the Cutie"
** Applebloom "persuading" Doctor Whooves to buy some apples.
-->'''Applebloom:''' How would ''you'' like to buy me some ice cream?[[labelnote:*]] Applejack promised her ice cream in exchange for helping sell apples.[[/labelnote]]
-->'''Doctor:''' (backing away) Sounds illegal...
-->'''Applebloom:''' Are you sure you don't wanna buy me any ice cream? Because I am the perfect height to reach your dangly bits, and I think we both know what I'm implying here.
-->'''Doctor:''' (getting nervous) Look, I'm really busy right now and I'm doing something really important. I have to save a kitty named Mr. Fluffles from certain doom.
-->'''Applebloom:''' Do not make me get persuasive with you!
-->'''Doctor:''' (panicked) Stay back! I can travel through time!
-->'''Applebloom:''' Well, good, you're gonna need that power, because ''I'' am about to kick your dick into last Thursday!
-->'''Doctor:''' I will cry!
-->'''Applebloom:''' [[KidsAreCruel Children have no empathy!]] I don't even ''care!'' Now you give me some friggin' ice cream!!
-->'''Doctor:''' Okay! Just take the money and leave me alone!
-->'''Applejack:''' Little lady, I don't know if I should be disappointed or impressed with your behavior. One thing's for certain though. You're fired.
* "Feeling Pinkie Keen":
** Pinkie's knowledge of the Hydra that lives "spitting distance" from Ponyvile, as Applejack so eloquently put it.
-->'''Pinkie:''' His name is "Richard", he is allergic to peanuts, and he has a birthday in three weeks.
** When Richard chases them to the ledge and it looks like the end for them, we get this little gem:
-->'''Pinkie:''' We will die as we lived, [[VerbalTic yes]]! Hating Fluttershy.
-->'''Fluttershy:''' I agree with that sentiment! I'M SO SORRY EVERYONE!
** This. Just... This:
-->'''Spike:''' Well, personally, [[TemptingFate I hope you never]] [[{{Foreshadowing}} become a Princess]], if I'm any indication of what it's going to be like for everyone.
-->'''Twilight:''' Eh, I wouldn't hold your breath on it anyway.
** The [=Q&A=] between Pinkie and Twilight as part of the latter's "research" on friendship.
-->'''Pinkie (shackled by the hooves with a skullcap on):''' You promised to Pinkie bondage! [[VerbalTic Yes.]]
-->'''Twilight:''' I did, and you're in bondage. I knew you'd go for it.
-->'''Pinkie:''' Okay, it is different, yes. When is Twilight in bondage as well?
-->'''Twilight:''' I'd ask how you think that's supposed to work, but I already know, sadly.
** The moral that Twilight comes up with at the end of the episode, after wearing herself out trying to figure out Pinkie Pie, is hilarious:
-->'''Twilight:''' You know what I think? I think the moral of this story is that the social sciences are retarded.
* In the remake of the episode, a frustrated Twilight starts to demand Pinkie explain when and how she could get a college degree, realizes how pointless asking Pinkie anything is, then redirects the question to Applejack, demanding she explain Pinkie for her.
* "The Show Stoppers"
** Working My Twerking. The entire song is gold.
** "I AM CALLING YOUR BLUFF! Tell them you were bested by a child. All is fair in money and politics."
* "A Dog and Pony Show"
** The entirety of the interaction between Rarity and the Diamond Dogs. Here's an example
-->'''Rover:''' [[HulkSpeak Pony make sandwich.]]\\
'''Rarity:''' No.\\
'''Rover:''' Curse you, Pony!
** And later...
-->''*two diamond dog mooks dig gems out of a cave column, which then collapses on top of them*''\\
'''Rarity:''' Di- Oh my god! Did those dogs just kill themselves by causing a cave-in?!\\
'''Rover:''' Yes.\\
'''Rarity:''' You are underground dwellers who dig tunnels to live! How could you ''not'' be mindful of cave-ins??\\
'''Rover:''' Pony. Dog has been digger for all Dog's life. Maybe when Pony has been digger for all Dog's life, ''then'' Pony will understand.\\
'''Rarity:''' That has got to be the most asinine thing I've ever heard. You know, I'm sure the old methods are wonderful, but there has got to be a way to dig for gemstones that isn't suicidal.\\
'''Rover:''' Pony dig now.\\
'''Rarity:''' No!\\
'''Rover:''' Why Pony no dig?\\
'''Rarity:''' Because I will die in a cave-in. [[TheReasonYouSuckSpeech How come everything you tell me to do is self-defeating? There's dull-witted and I don't even know what this is! How do you survive?! When you chew gum, does your brain forget to send signals to your heart?? This has got to be some kind of record for stupidity!]]\\
'''Rover:''' Pony make sandwich.\\
'''Rarity:''' I will ''never'' make you a sandwich!
* Fluttershy's fate at the end of "A Bird in the Hoof". [[spoiler:(Celestia banishes her and then locks her in a dungeon in the place she's been banished to)]]

!!From "Rainbow Dash Presents":
* From [[http://www.youtube.com/watch?v=_AbtCTbSWTk Rainbow Dash Presents]] ''Fanfic/{{Cupcakes}}''. It starts with Rainbow Dash deciding to put on a gorilla suit after reaching Sugarcube Corner and spirals into hilarious madness from there.
** Pinkie's rendition of ''Hooked On A Feeling'' in order to convince Rainbow Dash to come home with her. It doesn't work. What does? This
-->'''Pinkie:''' (Annoyed) [[spoiler:[[MightyMorphinPowerRangers Go go Power Rangers]] na na na na na na...]]
-->'''Rainbow Dash:''' (excited) Oh my gosh! Oh my gosh! Oh my gosh! How did you know!?
* From (ironically enough) ''[[http://www.youtube.com/watch?v=n7HrijDkfHc&feature=g-all-a&list=PL92BE27F054BDAA36&context=G2958739FAAAAAAAAAAA Rainbow Dash Presents]]: [[Fanfic/RainbowFactory Captain Hook the Biker Gorilla]]'':
** Thrackerzod's "I WANT TO BE AN OOMPA LOOMPA. Take me to them so the deed may be done..."
*** "The string is chafing me! THAT IS NORMAL."
** "Aurora starts with the test, but pretty much right away [[HumiliationConga she breaks one wing, dislocates the other, and chips four teeth.]] She does all of this during freefall, which I'm pretty sure wasn't possible beforehand."
** Almost the entirety of Ms. Blossomforth's dialogue.
-->'''Blossomforth:''' If you fly too far west, ''you will fail''. If you drop below the cloud layer, ''you will fail''. If you perform the exam in REVERSE, ''you will fail''.
-->'''Scootaloo:''' But what if we OPT outta taking the test?
-->'''Blossomforth:''' [[SubvertedTrope The exam will be offered again in the Fall]], and upon that exam... [[DoubleSubverted you will fail.]]
** Like, all of it.
-->'''Blossomforth:''' The educational system does not have time to understand its own intent.
-->'''Blossomforth:''' If this exam were fair, it would take all day.
** Mexican Surprise as [[RefugeInAudacity Pogo the Clown]]. That was infamous serial killer John Wayne Gacy's clown name.
-->'''Thrackerzod:''' Wait. Doesn't Willy Wonka kill all the children?
-->'''Surprise:''' O-Okay, hey, hey that's a good point, I'm not Willy Wonka anymore! I'm Pogo the Clown! You can trust Pogo! She's safe with kids!
-->'''Thrackerzod:''' I declare this cannot go wrong!
** Aurora's voice, which is an over-the-top impression of Creator/BillCosby. (though Greg had asked [=DeMarkus=] for Kermit the Frog)
--> [{{Beat}}] EVERYBODY PANIC!
** During their ''Series/LegendsOfTheHiddenTemple''-esque escape from the Rainbow Factory, the ponies get stuck in and ultimately fail at the [[MemeticMutation Shriiiiiiiine]] [[ThatOnePuzzle of the Silver Monkey]].
-->'''Applebloom:''' I'm trying to force it, but it won't go!
-->'''Thrackerzods:''' The head is backwards.
-->'''Scootaloo:''' Try jiggling it around.
-->'''Thrackerzods:''' The head is backwards.
-->'''Applebloom:''' Maybe it's broken?
-->'''Thrackerzods:''' THE HEAD IS ''BACKWARDS''!
** "There's this one kid with a magical glandular problem, and his butt's like, falling through the floor..."
** [[RunningGag "There's cookies and punch by the door."]]
** "Now I dressed up like Willy Wonka and a clown for nothing!"
** "You've been grounding up ponies, baby? Sure you haven't been grinding up ON ponies? HOO!"
* From ''Rainbow Dash Presents: Haunted Nightmare'':
** Any scene involving Zecora.
** Pinkie trying to [[CrossesTheLineTwice drug Twilight so she can have her way with her]].
** "[[Series/PeeWeesPlayhouse Meka Leka]] ''[[VerbalTic Yes]]'' Meka Heiny Ho! Meka Leka ''[[VerbalTic Yes]]'' Meka Heiny Ho!"
** Rainbow Dash goes to get a glass of water for Pinkie Pie. Literally ''one second'' after disappearing into Twilight's kitchen, she does [[NoodleIncident something]] to set off the smoke detector. And then a [[NoodleImplements jackhammer]]. And ''then'' machine gun chatter, complete with muzzle flash. And finally, a barking dog.
--> '''Rainbow Dash''' (off-screen): Twilight, do you have any ketchup?
** This exchange near the end of the video:
-->'''Zecora:''' I saved you from swamp gas!
-->'''Twilight:''' You saved me from... what?
-->'''Zecora:''' I am the deuess macarena!
-->'''Twilight:''' You mean DeusExMachina?
-->'''Zecora:''' [[CloudcuckooLander HEEEEEEEEYYY, MACARENA!]]
* In "Rainbow Dash Presents: A Beautiful Day In Equestria", Twilight's response to the shipping: [[ScrewThisImOuttaHere Running.]]
** And after the credits, we find out she wound up in a nightclub. Filled entirely with various apes.
*** Made even funnier when in the ending commentary we learn that, because Greg wrote the original, that is the canon ending to the story.
* Rainbow Dash Presents : My Little Dashie...
** The fact that a ComicBook/{{Batman}} plot was put into it.
*** With an added pinch of Franchise/HarryPotter for good measure.
** The reaction to Dash's memory loss.
--->'''Rarity:''' This is like, the seventh time. The Seventh Time!
** [[ProperlyParanoid The stinger at the end.]]
* Rainbow Dash Presents: Bittersweet
** Scootaloo and Applebloom checking out a website where unicorn stallions post pictures of their... ahem, [[DoesThisRemindYouOfAnything horns]].
-->'''Applejack:''' Young lady, I told you to keep this door open when Sco- ''(sees the laptop and goes wide-eyed)''- What are you lookin' at?!
-->'''Applebloom:''' [[TakeThat Stallions callin' out for help in the only way they know how?]]
** For that matter, Applebloom finding out Pinkie's dead, and taking it surprisingly well.
-->'''Applebloom:'' Wait, Pinkie's dead? ''[looks at AJ]'' Y'all said she went on vacation.
-->'''Rainbow Dash:''' Then... Why'd we hold a funeral?
-->'''Applejack:''' Dash! Darn it?
-->'''Rainbow Dash:''' Who did we bury?
-->'''Applejack:''' I don't wanna have to talk to my sister about death!
-->'''Applebloom:''' What's to talk about? Just last night, Ms. Cluckerbottom stopped layin' eggs an' we cut her head off.
-->'''Applejack:''' ''[flustered]'' Well, Scootaloo's parents probably don't wanna have conversations with her about things she ain't ready for.
-->'''Scootaloo:''' ''[*fiddling with the aforementioned laptop*]'' Look at this one, baby. Twelve inches.
** When AJ grabs the laptop and says the website isn't suitable, Scootaloo grabs and strokes AJ's tail, saying the website isn't suitable for anyone, and that's why it's the siren's call. AJ smacks her around the head with her tail.
** [[FunnyBackgroundEvent "What is he doin' with that spatula?"]]
*** Comes back at the end narration when Rainbow mentions that Applejack kind of forgot about Scootaloo being banned from the house. Applebloom is staring at something, horrified, as a spatula image twirls on screen.
** Included in Pinkie's Last Will and Testament is the demand [[spoiler:that Fluttershy's eulogy be drowned out by dubstep.]]
** [[spoiler:[[CallBack Pinkie Pie joined the Ghost Wonderbolts. It was awesome]]!]]

!!From other Spin-offs and One-shots:
* "Alicorn Day"
** Celestia's last assignment and the "lesson" Twilight learned from it:
-->'''Spike''': So, hey, you remember that letter she [Celestia] sent you where she asked you to get those pictures of Applejack's brother?\\
'''Twilight''': Yeah.\\
'''Spike''': And then she told you to write a critique of Mac's physical appearance?\\
'''Twilight''': Yeah\\
'''Spike''': And then she sent the critique to Mac?\\
'''Twilight''': ([[INeedAFreakingDrink *levitates a drink into her hoof*]]) Yeah.\\
'''Spike''': What'd you tell her you learned from that?\\
'''Twilight''' (in a monotone): A [[NoodleImplements Phillips screwdriver]] is persuasive, but not seductive; and also Rarity charges out the butt for stuff I really think she should do as a friend.

* "Investment Losses"
** Twilight and Rainbow Dash on the cloud from "Alicorn Day", when suddenly Twilight's wings disappear and she falls through the cloud.
** Aurora on the bus:
-->'''Aurora:''' Excuse me, weird adult talking to a kid. I'm supposed to avoid Stranger Danger situations. For that I need a pretty wide bubble without any strangers in it. Now, are you gonna have a Stranger Danger situation? ''[*the adults move*]'' Thank you! I can tell by the lack of proximity to me that you are probably OK adults.
** The fact that Aurora's mother, Knitty Needles, [[GenerationXerox sounds just like her daughter]] is rather amusing.
*** She even talks about going to [[CallBack the Embroidery Warehouse]].

* "Budget Impasse"

!!From "Lets Ramble Over [[VideoGame/FinalFantasyVI Pony Fantasy VI]]:

* The concept of Rainbow Dash doing a LetsPlay is hilarious in-and-of itself.
* Part 14 features a ponified version of the opera scene. It starts off with Rainbow Dash singing along to the opera. [[HollywoodToneDeaf And then Pinkie Pie joins in.]]
** Made even funnier when everyone begins arguing over whether or not they were singing badly.
* The whole "Fighting the Train" sequence in part 8. http://youtu.be/AnetOvT7L3I?t=11m35s

!! From "Let's Ramble Over VideoGame/FalloutNewVegas:

* Rainbow Dash deciding to name her character "QueenLatifa"
* The entire "[[CthulhuMythos Church Of Dagon]] Food Drive" segment.
--> '''Rainbow Dash:''' What ridiculous Cthulhu nightmare did I just escape from?! Hey kids, everyone get ready for the Church Of Dagon Can Drive! Kids that donate 20 cans will get a Church Of Dagon pocket knife. It's got the Church Of Dagon logo on it and everything! Kids that donate 50 cans will get a signed photograph of Dagon himself. And, if the school reaches its goal of 40,000 cans, Dagon will visit the school, live, in person, and will [[BreadEggsMilkSquick suck out your soul through your nose!]]