* The Joker's re-enactment of [[Film/StarTrekIITheWrathOfKhan KHAN!]] leading up to Deathstroke telling him to shut it or he'll gut him. What tops it all off is the Joker's smile afterward.
** Hell, [[https://www.youtube.com/watch?v=lKq4MhF_rrw EVERY moment with Joker in the game]]
* There's something about the absurdity of an unconscious Scorpion sitting in the passenger seat of the Batmobile as Batman drives to Gotham.