* At the beginning, Megamind comments that "It could be worse." Then notes that he's the one falling to his death. Well, guess it can't.
* The HamToHamCombat between Megamind and Metro Man at the beginning:
-->'''Megamind:''' In case you haven't noticed, you've fallen right into my trap!\\
'''Metro Man:''' You can't trap justice! It's an idea, a BELIEF!\\
'''Megamind:''' Even the most heartfelt belief can be corroded over time!\\
'''Metro Man:''' Justice is a non-corrosive metal!\\
'''Megamind:''' But metals can be melted, by the heat of re-''vahnge''!\\
'''Metro Man:''' It's ''revenge'', and it's best served ''cold!''\\
'''Megamind:''' But it can be easily reheated, in the microwave of evil!\\
'''Metro Man:''' Well, I think your warranty's about to expire!\\
'''Megamind:''' [[OverlyLongGag Maybe I got an extended warranty!]]\\
'''Metro Man:''' [[{{Metaphorgotten}} Warranties are invalid, if you don't use the product for its intended purpose!]]\\
'''Roxanne:''' Girls, girls, you're both pretty! Can I go home now?
* Roxanne's been kidnapped enough to be completely familiar with all of Megamind's deathtraps. Well, the "spee-yiy-dorr" is new.
** Roxanne's utter nonchalance while being kidnapped.
-->'''Metro Man:''' ''[[LargeHam Don't panic Roxanne!]]''\\
'''Roxanne:''' I'm not panicking. ''[Is ignored]''
* There's also her request to have her "frequent kidnap victim card" stamped. Megamind insists that they discontinued that promotion.
* Megamind gets booed by the audience and what's his response? "[[InsultBackfire Booooo! Yes, I can play along, too!]]"
* Roxanne also has one when [[spoiler: she finds out Metro Man is alive and won't stop Titan]]:
-->'''Roxanne:''' I say we get a bunch of [[RayGun ray guns]], [[GangstaStyle turn 'em sideways]] and just go all ''gangsta'' on him.
** [[DateNight That's a kill shot!]]
* "Use the forget-me-stick!" "Oh, right." '''(WHACK!)'''
* "Good luck, fellas!" "We're gonna ''die''!"
** Plus the Warden's little "well... yeah." nod.
* "I'm not allowed to insult guests directly." Oh, [[{{Jerkass}} Bernard.]]
* Megamind, in disguise as Space Dad, gives Hal his superhero suit.
-->'''Megamind:''' You shall be called... Titan!\\
'''Hal:''' Why [[FunWithHomophones Tighten]]?\\
'''Megamind:''' It was the only name I could trademark.
* Any time Space Dad opens his mouth. He's basically [[{{Film/Superman}} Jor El]], but voiced by a [[TheGodfather very different Marlon Brando character]].
--> "Unfafomble... unfaf... without fathom"
* When Titan rips the door off the invisible car, Megamind hides by grabbing onto it. When it becomes visible, Titan notices Megamind on it through the door window. Megamind's nervous laugh and expression as he pointlessly clicks the lock on the door is just great.
** Well, really any of Megamind's expressions are fine examples.
*** Agreed! Another mention should go to his particularly epic OhCrap face after Roxanne explains how she found his hideout.
* One of the greatest ironies in the entire film comes after Titan learns that Space Dad had really been Megamind under a holographic disguise the entire time. Titan is furious, not only for Megamind deceiving ''him'', but for also deceiving ''[[PaperThinDisguise Space Step Mom]].''
* When Roxanne blows a dangling spider into Megamind's eye, and Minion tries to help by ''punching him in the face'', and then ''stomps on his head''.
* When Megamind and Roxanne are investigating the school.
-->'''Roxanne:''' There are ice cubes in this glass.\\
'''Megamind:''' [[CaptainObvious Yes, that's what happens when water gets cold.]]
** Not to mention [[spoiler: Metro Man coming in behind them, eating a sandwich and silently freaking out as they continue to remark on the state of the ice. Their reaction to turning around and seeing him is priceless.]]
** Megamind's reaction, complete with PuppyDogEyes:
-->'''Megamind:''' [[OurGhostsAreDifferent Are you a ghost... ?]]
* The entire scene where Roxanne infiltrates Megamind's lair.
** Special mention goes to when Roxie finds the secret entrance: a doormat labeled "Secrit Entrance", courtesy of Minion.
-->'''Megamind''': ''Minion!''\\
'''Minion''': (meekly) I kept forgetting where it was.
** Another special mention to "Bernard" fighting Megamind.
-->'''"Bernard"''': [[ButHeSoundsHandsome I tried my best, but he was too fantastic!]]
* When Megamind enters upon City Hall as AC/DC's ''Highway to Hell'' is playing... he orders Minion to cut the music... and Minnie Ripperton's ''Loving You'' starts playing!
** Even funnier is the fact that "Highway to Hell" [[SoundEffectBleep switches to "Loving You" every time the word "Hell" is uttered.]]
*** Actually, it switches ''to'' a "Hell" just before Minion breaks it to stop it. Well, an "ell", anyways.
* When Titan tells Roxanne that she lives in a fantasy world and tells her that she shouldn't believe in things like justice, because they are as real as the Easter Bunny, the Tooth Fairy, and the ''[[EskimosArentReal Queen of England]]''.
* Also, this exchange between Roxanne and Megamind when [[spoiler: Megamind is fighting Titan]]
-->'''Roxanne:''' Remember the night I dumped you?
-->'''Megamind:'''You're bringing this up ''now''?
* "The ''sun'' is warming up?!"
* The entire opening.
* Minion [[spoiler:FakingTheDead for a dramatic death scene]]. Made utterly hilarious by Megamind's utter nonchalance.
* Any of the dancing scenes.
* This exchange:
-->'''Megamind, Pretending to be Bernard''': "I was just yelling at... my mother's... urn."\\
'''Roxanne''': "Well, hurry up."
* And when Megamind/Bernard asks if Roxanne would still like him if he (just as a random example) was bald and had the complexion of a 'popular primary color'. Roxanne's response?
-->'''Roxanne''': "You don't judge people based on how they look."\\
'''Megamind''': (Adopts a look of relief.)\\
'''Roxanne''': "You judge them by their actions."\\
'''Megamind''': (Look of relief vanishes as the implications of that sink in.) "...Well that seems kind of petty."
* Musicman.
-->'''Roxanne:''' 'Music Man'?\\
'''Metro Man:''' That way I can keep my logo.\\
'''Roxanne / Megamind:''' ''[In unison]'' Come again? / Cuzzawha?\\
'''Metro Man:''' [[LargeHam Finally, I was free to get in touch with my TRUE POWER!]] Weaving lyrical magic. Check ''this'' out.\\
''[Playing music ... badly]''\\
'''Metro Man:''' "I have eyes / that can see / right through leeeeeaaaaad."
* Black Mamba. Complete with a fanfare reeking with MundaneMadeAwesome.
* [[OwMyBodyPart OW, my giant blue head!]]
* When they go to find clues at [[spoiler: Metroman's hideout]], they find all the things he kept, and Megamind eyes one of the old capes.
-->'''Roxanne''': Hey, come over and look at this!\\
'''Megamind''': (comes crashing in from the right and springs back up, wearing the white and gold cape) ''What is it?'' What did you find?
-->'''Roxanne''': (wearing the quintessential "wtf" face) Um...
* "Okay, Minion! You were right! I was...less right!"
* Megamind fighting [[spoiler: Titan for the first time]]
-->'''Megamind''': Now that's the spirit! Parry. Thrust. Parry again. Now it's time for some witty back-and-forth banter. You go first.\\
[[spoiler:'''Titan''']]: [[{{Kiai}} AHHHHHHHHHHHH!]]\\
'''Megamind''': Okay, look, I'm not sure where to go with that.
* "I wouldn't stay for more than 2 minutes 37 seconds if I were you! We're having the walls and ceiling removed!"
* The after-credits sequence. [[spoiler:The dehydrated Bernard pops out of the washing machine]] and Minion just responds by admonishing Megamind:
-->'''Minion''': Sir, you really need to clean out your pockets more often!
* When Megamind busts out his MotionCaptureMecha and the rock music to battle Titan the first time, Titan doesn't show... cue the TimePassesMontage as Megamind's rock music shuffles through the whole playlist [[BrickJoke until it lands on "Loving You" again.]] [[FridgeBrilliance The whole robot is slumping over BECAUSE it's a Motion Capture Mecha.]]
** And he finally resorts to playing with a nearby parked car as if it were a life-sized Hot Wheels car.
-->'''Megamind''': Oh no I'm going to crash! Ahh! ''['Drives' the car into the reflecting pool outside City Hall]'' Glub glub glub!
** I always found the part where the robot shakes its finger as Megamind calls Hal "rude" strangely funny.
* Megamind "losing his cool" while the Metroman Museum is blowing up behind him.
-->'''Megamind:''' Aaaah! Oh! I'm too close! I'm genuinely scared right now! Oh! I hope no one can see this! Aah!
* "I'm in a heated existential discussion with this dead-eyed plastic desk toy!"
* The entire press-conference after Megamind vanquishes Metroman, which opens with a stubborn sound system insisting on playing "Loving You" to Megamind pretty much admitting that he's got no clue what he's doing:
-->'''Roxanne''': I'm sure we'd all like to know what you plan to do with us and this city!\\
'''Megamind''': Good question... glad you asked that. Imagine... the most horrible, terrifying, evil thing you can think of. And multiply it -- '''''by six'''''! In the mean time, I want you to carry on with the dreary, normal things that people do. Let's just have fun with this, come on! And I will get back to you!
* Minion, upon being asked if he ever imagined the day Megamind won:
-->'''Minion''': No way. Not at all, sir. Never. Never in a million years. ''[Notices Megamind's glare]'' I mean... [[BlatantLies yes! I did!]]
* When Megamind is riding his bike, it has stabilsers. Simple, but very funny.
* When Megamind, disguised as Bernard, is trying to get Roxanne out of his lair.
-->'''Megamind:''' How about this one? This looks exciting!\\
'''Roxanne:''' Bernard, it says "Exit."\\
'''Megamind:''' ...Which is short for "Exciting!"
** Later, Roxanne tries to leave through the door. It leads to a pit with alligators, squeaky toys, dance music, and a disco ball.
-->'''Roxanne:''' Bernard! You were right about that room being exciting!
** Megamind being forced to blow up his brain bots with dynamite:
-->[loud whisper] Daddy's sorry!
* When Megamind is trying to intimidate Roxanne with his various traps/devices, he eventually ends up spinning a wheel whilst exclaiming "What does this thing do?!"
* This exchange during Megamind and Minion's [[spoiler:"break up scene"]]:
-->'''Minion:''' Well, good luck with your date!\\
'''Megamind:''' I will!\\
'''Minion:''' That doesn't even make any sense!\\
'''Megamind:''' I know... !
* Hals/[[spoiler:"Titans"]]'s very first training session is incredibly hilarious, even if it is {{Foreshadowing}}.
** The look of horror on Space Dad's face as [[spoiler:Hal]] melts away the Megamind mannequin sells the scene.
* The scene where Megamind announces his not-quite-plan for Metro City, and a good chunk of the attendees are recording the whole thing on their camera phones.
* Minion's perfect girly scream after Megamind says [[spoiler: "Maybe I don't ''want'' to be the bad guy!"]]
-->'''Minion:''' ''(thunderstruck)'' ... who ''ARE'' you?!
* Roxanne and Megamind are both talking to Metroman's statue.
-->'''Roxanne:''' Evil is running rampant through the streets.\\
'''Megamind:''' [[VictoryIsBoring I am so tired of running rampant through the streets!]]
* The all-too-familiar training course ([[VideoGame/DonkeyKong with girders and flaming barrels tossed at Titan by Minion]]) is worth a chuckle.
** He's even imitating Kong's movements.
* The "No You Can't" sign.
* When the syringe gun indicates that it needs to warm up, just like the sun death ray:
-->'''Megamind:''' [exasperated] Minion, if I live, I will kill you.
* "He's not used to ''positive'' feedback!"
* How [[spoiler: Metro Man]] just [[ShootingSuperman stands there without so much as flinching]] as Roxanne throws one thing after another at him in a fit of anger.
-->'''Roxanne:''' ''(throws a trophy at [[spoiler: Metro Man]])'' How could you ''do'' this?! ''(throws microphone)'' The people of this city ''relied'' on you ''(throws an amplifier at him)'' and you ''deserted'' them! ''(smashes guitar over his head)'' You left us in the hands of - of - ''him''! ''(indicates Megamind, then hastily turns to him and adds)'' No offense.\\
'''Megamind:''' ''(hastily)'' No, I'm with you.
* At the beginning, the criminals doing flashcards with baby Megamind. When they show him a card of a police officer, they shake their heads and Megamind mimics the motion, and when they show him a card of a criminal, they nod and Megamind does the same.
* Megamind's comment about being eight days old, and still living with his parents. "How sad is that? Clearly, it was time to move on."
* The very first entrance into Halís apartment: Megamind enters through the door by blowing it up. Minion then promptly installs a new one.
** What makes this even funnier is the fact that the door is unlocked [[note]](Hal to Roxanne, one scene earlier:) ďIíll leave the door unlocked in case you want to check on me.Ē[[/note]].
* It's there for about a second, but right when Metro Man is giving his big speech during the unveiling of his museum, one person (presumably Random Citizen) actually shrieks with excitement just to hear what Metro Man has to say.
* There's something incredibly funny about the way Metro Man reveals his big epiphany: "[[LargeHam And THAT'S when I DECIDED...]] to fake my death."
* "Formal speed walking"