* Toward the end of the game, Mario and company are attempting to rescue Peach from [[spoiler:[[BigBad Bowletta]].]] Fortunately, Toadsworth had packed an extra dress for Peach in case of ClothingDamage. Mario's solution? [[spoiler: [[DisguisedInDrag Dress]] [[ButtMonkey Luigi]] [[DisguisedInDrag as Peach]],]] then attempt to [[spoiler: pass him off as the "real" princess. Luigi]] goes the extra mile; [[spoiler:[[NoblewomansLaugh he mimics Peach's voice]] with surprising accuracy]], and even [[spoiler: covers his face with his hands to hide his mustache]]...until [[spoiler: he is in Bowletta's [[CoolAirship cruiser]].]]
-->'''[[spoiler: Bowletta:]]''' By the way, Princess...why are you [[spoiler: covering your mouth with your hands?]]
-->'''Fawful:''' Do you hide something?
-->''[[spoiler: Bowletta fakes Luigi out, and Luigi uncovers his face.'']]
-->'''[[spoiler: Bowletta:]]''' Fawful...did you see that?''
-->'''Fawful:''' Yes. [[spoiler: Mustache.]]
-->'''[[spoiler: Bowletta:]]''' Fawful...
-->'''[[spoiler:Bowletta:]]''' ...fire at will.
* Hermie III thinking that Mario is SantaClaus because of his mustache and red clothes.
* The whole sequence after Mario's consumed the Invincishroom. Luigi moving very slowly due to fear and being hypnotized into thinking that he's Mario in particular.
* Mario running around his underwear.