* Every single [=Fawful/Bowser=] encounter. Fawful turned his Engrish UpToEleven (and passed on his "unique" language skills to [[spoiler:Midbus]]), and Bowser gets the best comebacks ever.
--->'''Bowser:''' Hey, what's your beef?
--->'''Fawful:''' Beef? I am lacking in beef. Fawful is beefless.
** And after [[spoiler:Chakron]]'s explanation of how Chakroads work:
--->'''Bowser:''' TOO MANY WORDS.
** [[ItMakesSenseInContext "Ready for massage duty?"]]
** Before the battle against Alpha Kretin, Luigi asks Starlow to fight with Mario. Starlow promptly responds by smacking him into the boss himself.
** While chasing [[spoiler: Wisdurm]], Luigi falls in a mucus pit. While Mario and Starlow are discussing how to proceed, he falls in again.
** "Bowser keeps appearing like a rabbit from the ugly hat of a magician who stinks!"
*** From that same cutscene:
--> '''Fawful''': You are a nuisance barge barging in at a critical time for us!
--> '''Bowser''': Nuisance barge? [[EvilLaugh GWAHAHAHA!]] Buddy, you're like a nuisance SUPERTANKER!
* When Fawful forcibly extracts Peach from Bowser's body, the orb containing her form slowly drifts down toward Midbus... and suddenly darts over and deposits her on Fawful. Fawful is barely able to hold her and asks for help. Midbus ignores him and walks off.
** Bowser's response? "Hey, that's mine! [[AssShove Put that back!"]]
* Starlow trying to convince Luigi that he is not cold (and Luigi still being totally cold)
** She just ''pops out and stares at him'' the last time. Just ''[[DeathGlare stares.]]''
** The kicker? After the last stare Luigi rejoins Mario and proves to be a smartass by shivering once more.
* Mario and Luigi trying to catch [[spoiler:Durmite/Wisdurm]] is pure slap-stick.
** Especially when it farts on him.
* The look Bowser gives when he's about to push a Fawful statue right to get access to the underground section of his castle is pure gold.
* Midbus unleashing Junker on the bros.
--> '''Midbus''': No one pressed 'dance'! You are broken!
** It's even funnier when you realize that ''DANCE'' DOESN'T MAKE SENSE.
* After the Star Cure is born, the player may notice that one NPC in Toad Town still appears Blorbed. Talking to him gives you this-
--> "Oh, well, hey there, Mario. They cured the blorbs, huh? What, me? This is what I normally look like."
** "MIRACLE TOADLEY CURE!"
* After the Dark Star explodes, we get this exchange between Bowser and Starlow:
-->'''Starlow''': The Dark Star. It's finally gone.
-->'''Bowser''': Yeah. Pretty tough dude, all around.
-->'''Starlow''': And the princess? Is she okay?
-->'''Bowser''': Oh yeah! I forgot! Now I can kidnap her!
-->'''Starlow''': [[WhatTheHellHero Oops. I spoke too soon.]]
* And now for unspoilered [[UnreadablyFastText rapid-fire dialogue!]]
** When Bowser can't find a seat at the theatre [[spoiler: set up in his own castle after Fawful seized it]], he goes to complain. (Genders assumed based on the tone of their [[SpeakingSimlish text sound effects]].)
-->'''Boss Goomba''': Is there a problem?
-->'''Male Usher Goomba''': Oh, Boss, phew! This guy...
-->'''Female Usher Goomba''': So this big galoot here, right? He's all like, I have no seeeat! No seeat! And he won't shut up!
-->'''Female Usher Goomba''': But he won't stop, and we're all, we just can't do it, buddy, OK? So we have no idea what to do.
-->'''Female Usher Goomba''': And this lug's getting surlier. He's not going away. And he has no seat! Nothing! Not a chance!
-->'''Female Usher Goomba''': So we do some solid thinking and totally fail. No big seats! I mean, we got small seats!
-->'''Female Usher Goomba''': [[UnreadablyFastText We can do a small seat, sure, but this guy's huge, so he's not gonna fit in a small one! Please!]]
-->'''Female Usher Goomba''': [[UnreadablyFastText And it's kinda bugging me, 'cause I'm on my feet all day! I mean, I'd LOVE to sit in ANY seat!]]
-->'''Female Usher Goomba''': [[UnreadablyFastText It's like, hey, pal, how about you try our job just once, y'know? It'd break him in two seconds!]]
-->'''Female Usher Goomba''': [[UnreadablyFastText All the suffering in the world, and this guy's seating problem is urgent? Nooooooo!]]
-->''*{{Beat}}*''
-->'''Female Usher Goomba''': Ooooo!
-->'''Other Goombas and Bowser''': '''[[VisibleSilence ...]]'''
-->'''Boss Goomba''': Uh... Right. Gotcha.
-->'''Boss Goomba''': ''*thinking*'' [-Whoa.-]
*** This scene is very much OOCIsSeriousBusiness for the Goombas, as this particular one was previously notable for [[TheRuntAtTheEnd being late to every line]].
** Like other minions, once Bowser frees the Magikoopas, a short skit occurs. Though not as quick as the aforementioned goomba, some of the text gets a bit quick. (Magikoopas numbered according to [[ItMakesSenseInContext on-screen placement]].)
-->'''Magikoopa #9''': We who are not worthy of the mighty Bowser's personal rescue must honor him...
-->'''Magikoopa #8''': We, the Magikoopa corps, have attempted to find a suitable way to express our deep gratitude...
-->'''Magikoopa #5 (Jerry?)''': ''*turns right*'' And so we hereby present an invigorating and inspiring dance of utter joy and happiness...
-->'''Magikoopa #6''': ''*turns right*'' No, wait. We talked this over, Jerry, remember? We were gonna sing a song of Bowser praise?
-->'''Bowser''': Forget it, minions! Let's get a move on!
-->''*Bowser turns around*''
-->'''Magikoopa #7''': ''*turns left* Just a sec. I thought we'd build a monument to his strength!
-->''*Bowser starts walking out*''
-->'''Magikoopa #4''': ''*turns down-left to #7; Bowser stops after a couple steps*'' No, we were going to make his favorite food, right?
-->'''Magikoopa #11''': ''*turns up-left to #6*'' Not me--I voted for polishing his horns with velvet shammies...
-->'''Magikoopa #2''': We DECIDED this, remember? It was foot massage all the way!
-->''*Bowser turns*''
-->'''Magikoopa #10''': ''*turns up*'' I still think that taking lutes and singing praises is sweet...
-->'''Magikoopa #8''': ''*turns left*'' [[UnreadablyFastText Is]] [[{{Squick}} flossing his teeth]] [[UnreadablyFastText still on the table? Because they are NASTY.]]
-->'''Magikoopa #3''': ''*turns down-left to #6*'' [[UnreadablyFastText No kidding. In fact, we should do a full orthodontic overhaul.]]
-->'''Magikoopa #12''': ''*turns up-left to #7*'' [[UnreadablyFastText OK,]] [[SeriousBusiness we'll have Alpha Team hit the cavities, then deploy two--]]
-->'''Bowser''': ''*jagged [[SpeechBubble speech balloon]]*'' QUIET! MOVE IT!
-->'''Magikoopas''': ''*turn to face Bowser again, jagged speech balloon, enlarged text*'' KOO-RAH!
* The look on Bowser's face when Starlow refuses to find his safe combo until he asks nicely.
* During the Junker boss fight, after sustaining enough damage, Junker goes up to Luigi and sucks him toward his vacuum hand, and throws him at the Trash Cans that accompany the Junker. Most of the time, he goes into one of the cans, where he must be rescued by Mario. [[spoiler:On occasion, he doesn't go into a can at all, and bounces off of the rim.]]
** The stupid little fist pump the Junker makes when Luigi lands in a can.
** If Luigi is still in the trash cans after a bit he'll wave to Mario, and then the lid slams down on his fingers with him groaning.
* How about the simple fact that Bowser's battle tutorial is Fawful observing his battle with Midbus, warning Midbus of Bowser's attacks and demanding Bowser ''not'' do certain action commands.
* After Mario and Luigi beat the BonusBoss [[spoiler:Shroobs]] Mario quickly tells Starlow about them and how it was more or less Luigi [[spoiler:(Baby Luigi to be more exact)]], who saved them all. She doesn't believe that at first and she actually asked how Luigi did it, [[spoiler:"ferociously crying" - and she's dead-on right!]]
* Bowser's reaction when he sees the train tracks through his secret vault (and the train tracks themselves). Made even funnier by what he says before going inside:
-->"[[TemptingFate This is one place that stupid Fawful NEVER could break into]]!"
* When fighting Scutlet, he'll occasionnally [[spoiler: spit out Starlow and ''throw her at the Mario brothers'' as an attack. You must knock her right back at him to counter. Understandably, she'll be pissed]].
* Hell, the first and last giant battles themselves are so weird they're funny. I mean, [[spoiler: Bowser is fighting ''his own castle'' and ''Peach's Castle'']], for crying out loud!
* Speaking of the first giant boss, [[spoiler: it ends up getting so damaged it recieves InstantBandages. Yep, a ''castle'' recieves ''InstantBandages'']].
* When Bowser [[ManOnFire literally burns off calories]] by sprinting on a treadmill. He then accidentally sets off a Bob-omb before reading the sign that says he's in a Bob-omb depot...not noticing the Bob-omb walking to a pile of boxes filled to the top with Bob-ombs. His reaction when it all dawns on him?
-->"[[ThisIsGonnaSuck Crud]]."
** And the Bob-omb antics get an encore when he yells at his Bob-omb squad to quiet them down...and accidentally breathes fire on them setting off their fuse. His reaction just ''screams'' OhCrap!
* There's a hilarious reveal in a scene involving the green sock-like Sockops when Luigi is trying to save Mario from them. One of the Sockops has his name revealed. The name? [[MythologyGag Kuribo]].