* It '''starts''' off with a crowning moment of funny, showing that, despite surviving Nazi attacks and {{Temple of Doom}}s, Indy could not work his way around his own college without incurring a series of DisasterDominoes -- including getting conked on the head by a falling totem pole, being slammed through a thin ceiling via a bookcase, confusing [[CatScare a real cat for a statue, and getting startled]] enough to fall into a coal chute! The Talkie version makes this even funnier.
* When Indy interrupts Sophia's show in New York by sending the Nur-Ab-Sal puppet out too early:
-->'''Sophia''': Er... may I present NUR-AB-SAL, the great Atlantean god of... of...\\
'''Indy''': Deceit!\\
'''Sophia''': Deceit! Thanks Indy! (''beat'') [[DoubleTake INDIANA JONES]]? You've got some nerve!\\
(''The Nur-Ab-Sal puppet bursts into flames.'')\\
'''Sophia''': Oh, great... Go back, you big Jack-o-lantern!\\
(''The puppet is taken back offstage'')
* If Indy examines the screws in the bookcase at the Barnett College Library, he determines that it's "Early twentieth century, I believe."
* Early on, Indy has to go through all sorts of hoops to get to a Mayan pyramid... only for Sophia to show up on the other side after Indy told her to wait. He asks how she got there only for her to say she found a path.
* While in the Monte Carlo hotel room, if you turn off the lights and use the flashlight, Indy starts making shadow puppets on the wall.
--> '''Indy:''' "And here's Nur-ab-sal."
* In Algiers, if you have Indy look at Al-Jabbar's camel repeatedly, he debates with himself over whether it's a camel, a dromedary, a lama, or a llama with two l's, before deciding it's a camel, and that he's going to name it George. The "Look at _" text changes with each line.
** And if you return to the camel after visiting the dig site nearby, Indy will see that the camel has escaped. "Or was he a dromedary?"
* In any dark area, your "look" command is automatically replaced with a "touch" command. And should you actually use this command on Sophia... SLAP!
* [[GettingCrapPastTheRadar "Is that a ship rib in your pocket, or are you just happy to see me?"]]
* When using the surveyor's transit in Crete, you can see Sophia waving at you if you were looking at her through the sights.
* Arnold's the singing Nazi's last words: "YODEL-OOO-URK!"
* The single best HaveANiceDeath message resulting from losing a fist fight ever? Possibly.
--->Indy's failure to subdue a sixty-year-old U-Boat captain allowed the Nazis to conquer the world.
* An ancient Atlantean guard robot stomping a Nazi to death while [[SoundtrackDissonance the cheery marching band]] [[Film/IndianaJonesAndTheLastCrusade from the third film]] [[SoundtrackDissonance plays in the background]]. The [[OhCrap horrified expression]] the Nazi wears a split second before the first stone foot lands on his head makes it all the more hilarious.
* This video: http://www.youtube.com/watch?v=KnH6gxxsVBY