* Scamper's DeadpanSnarker lines. Creator/SteveBuscemi was clearly enjoying himself!
** "I'm sorry, I thought we were listing all your failures."
** "..., Brian." "My name's not Brian!" "Then you must have his jar!"
** "She must be collecting a club of flowers to beat us with."
** "What, like this is the first time I've gnawed my own feet off?"
** "When I finally decided I wanna live."
** "What's next; piggybacks?"
* [[CloudCuckooLander Brain]], in general. Helps that he's voiced by Sean Hayes.
** "Bring me back a toy!"
** The bit with the ribbon.
** After being called an imbecile: "Oh, parle Italian? Taco grande to me too."
** "I wanna live forever. I got plans! I got dreams! I got...a squeaky wheel."
** "Is french fries an idea?"
** When Brain is using Eva's pendant to hypnotize Igor, it fails. So instead, he ''whacks Igor on the head with it''.
--->'''Igor''': [[LampshadeHanging Are you actually trying to hypnotize me, Brain?]]\\
'''Brain''': Yeeeess... But if it's not workin', then how 'bout ''THIS?!'' ('''''*WHACK!*''''')\\
'''Igor''': OW!
* The off-hand way Doctor Schnaudenfreude talks to his Igor.
-->'''S's Igor:''' Master! I looked inside Dr. Glickenstein's castle and didn't see anyone. But that's not all...
-->'''Schnaudenfreude:''' Yes it is, because your voice is ''annoying.''
** The best part is that he stops somebody doing pointless exposition in the funniest way. And really, that was all Schnaudenfreude needed to know.
* "Get me a tub of eyeliner, a pound of lipstick and, if all else fails, [[BreadEggsMilkSquick the severed head of a super-model!]]"
* Scamper's [[DeathSeeker multiple]] [[WhoWantsToLiveForever suicide]] attempts.
* The GettingCrapPastTheRadar one-sided conversation Brain has with the fly-man.
* After Igor creates life:
-->'''Igor''': Blind orphans? She's ''evil''! Which is [[MadScientist go]][[MorallyAmbiguousDoctorate od]], but--blind ''[[EvenEvilHasStandards orphans]]''?
* The part right before the orphans go on stage. One little girl has her dress on backwards.
-->'''Scamper''': What are you, blind?!
* Scamper's introduction: "Good evening, frieeeeeends!"
* This gem:
-->'''Igor''': PULL THE SWITCH!\\
(''Nothing happens. He looks over at Scamper, standing by the switch'')\\
'''Scamper''': Do ''not'' yell at me.\\
'''Igor''': Oh, I-I'm sorry. I just... pull the switch? (''No response'') Please pull the switch.\\
'''Scamper''': That's better.\\
'''Brain''': Wait, why does HE get to pull the switch?\\
'''Igor''': Stop! You can both pull the switch!