* Any time Shigeru says some variation of [[OncePerEpisode "I have a boner"]]
** Doubly funny when you consider his original characterization as {{Asexual}}.
* "ITS AN ANGEL!"
** "IT'S A GUNDAM!"
*** "IT'S AN [[{{Engrish}} ANGERU!]]"
**** "ANGEL IT'S A!"
***** "OH MY GOD IT'S GODZILLA!!"
* Gendo's utter inability to identify the Angels, always calling them Franchise/{{Godzilla}}. And he suggests using the fish trap from the [[Film/{{Godzilla 1998}} American Godzilla]] movie against the Angels.
* Every conversation involving Gendo, particularly if it also involves Fuyutsuki.
* [[CodeMent "ACCORDING TO MY CALCULATIONS, YOU'RE A FAGGOT!"]]
* From Episode 4, Shigeru's explanation of NERV's situation against the Angel currently boring a hole straight towards the Geo-Front:
-->'''Shigero:''' According to ''my'' calculations, my massive boner is ready for penetration.
-->'''[[OnlySaneMan Misato:]]''' Can you ''please'' go a God-damn day without referencing your boner?! What can you tell me about the Angel?
-->'''[[TemptingFate Shigeru:]]''' [[DoubleEntendre The Angel is currently drilling hard and fast into us with it's long rod, expected to penetrate our barriers. With that deep hole bored into us, we'll be exposed right from behind against it's massive load-out.]]
-->''{{Beat}}; Misato's [[EyeTwitch nostrils flare and her eye twitches in irritation]].''
-->'''[[TranquilFury Misato:]]''' Makoto?
-->'''Makoto:''' Yes Ma'am?
-->'''[[TranquilFury Misato:]]''' Slap him.
-->''Audible slap is heard, along with Shigeru yelping in pain, as Misato's eye twitches again.''
* Rei, unlike her canon MysteriousWaif personality, is a master-class abusive DeadpanSnarker who does not put up with Shinji's shit.
-->'''Shinji:''' So they expect me to pilot it again? Really?! After all I've been through?! After all the pain?! They just want me to-
-->'''Rei:''' You know what you need to do?
-->'''Shinji:''' Wha-?
-->'''Rei:''' Get some tampons, drink a bit of cranberry juice, and go see your gynaecologist. I think you're starting to menstruate again.
-->'''Shinji:''' [[ComicallyMissingThePoint How can I menstruate if I have a penis?]]
-->'''Rei:''' [[AskAStupidQuestion That's a good fucking question.]]
-->'''Shinji:''' So you really don't care if they just use me or you to pilot until we die?!
-->'''Rei:''' I want you to do something.
-->'''Shinji:''' Uh... Okay...
-->'''Rei:''' Look at my face.
-->'''Shinji:''' Okay...
-->'''Rei:''' Just look at it.
-->'''Shinji:''' Rei...
-->'''Rei:''' No, no. Keep looking.
-->'''Shinji:''' I ''am'' looking!
-->'''Rei:''' No you're not. Look harder.
-->'''Shinji:''' Is there a point to this?
-->'''Rei:''' Yes. So look as hard as you can.
-->'''Shinji:''' Alright!
-->'''Rei:''' Focus on every part of my face.
-->'''Shinji:''' Alright, alright, I'm doing it!
-->'''Rei:''' Now. Does it look [[OverlyLongGag like I give a fuck?]]
-->'''Shinji:''' (''beat'') [[SarcasmBlind Are you coming on to me again?]]
* Asuka as a... [[CrossesTheLineTwice permanently menstruating, screaming psychopath]].
-->'''Asuka:''' I'M PERMANENTLY MENSTRUATING!!!!!
-->'''Shinji:''' Is she okay?
-->'''Asuka:''' I. DON'T. KNOOOOOOOWWWWW!!!!!
-->'''Shinji:''' What does that even m-
-->'''Asuka:''' AAAAAAAAAAAAAAAAAAAAAHHHHHHHHHH!!!!!!!