* The "interactive" dildo in [[http://www.youtube.com/watch?v=G_PwUHGjJzU This video]].
** '''Duke:''' ''You wanna touch it, don't you?''
* Upon seeing a giant three-breasted alien monstrosity, most people would probably run and hide. Duke's response?
--> "[[AnythingThatMoves Hell, I'd still hit it.]]"
* Duke engages in a little LampshadeHanging:
-->'''Twin:''' "What about the game, Duke? Was it any good?"
-->'''Duke:''' "Yeah, but after 12 fucking years it should be."
* When Gearbox had to delay the game ''[[HistoryRepeats again]]'' by one month, to June 10th in Europe and June 14th in the US, Gearbox released [[http://www.youtube.com/watch?v=G6fFKkmIrWM this video]] announcing the delay, with the {{Tagline}} [[{{Pun}} "Duke never comes early."]]
* [[http://www.youtube.com/watch?v=tRBfzoqOZcs This]] new trailer really puts strain on the definition of "Mature humor"
--> '''Duke:''' ''Combo!'' ''Double Combo!'' ''Triple Combo!'' ''Super Combo!''
** And yes, there's an achievement/trophy tied into finding and stealing a piece of poo. Behold, [[http://www.xbox360achievements.org/game/duke-nukem-forever/achievement/53792-Turd-Burglar.html the Turd Burglar.]]
* During a point in the demo, Duke is allowed to draw a 'plan' on a whiteboard for some soldiers. No matter how nonsensical a drawing you make or how much of the original plan you replace, the soldier observing will respond with amazement.
--> '''Soldier:''' Why didn't we think of that!? '''WHHHHHYYYYYYYYY!?'''
** Alternately:
--> '''Soldier:''' I have no idea what that means, but if I did, I bet that guy over there would still have his arm. And at least one ball.
* Running an enemy over with Duke's monster truck:
--> Beep beep, motherfucker!
--> Frickin' jaywalkers.
--> So much for anti-lock brakes.
* When Duke gets shrunk by a trap in "The Lady Killer" he meets a kid who says he's "the coolest toy ever." Duke's response?
-->"Hey, kid. I'm not a toy. I'm an ''action figure''!"
* Later, while shrunk, Duke ends up fighting some rats who mistake him for an easy meal.
-->"Now those were some [[Film/ThePrincessBride rodents of unusual size.]]"
* Later in that chapter, Mc Ready berates Clarke for saying "Let's do some damage!". Clarke then comes up with "Let's rupture some spleens!". mc Ready's reaction is a dumbfounded "Why are you such a douche?"
** This is's one of the few lines the italian dub improved:
--> '''Mc Ready:''' (''exasperated'') ''Why'' are you such an ''asshole''?
* Hearing shrunken Duke exclaim "motherfucker!!!" in his squeaky voice as he falls down an air vent.
** Upon being first shrunk; "The ''fuck''...?"
* The text on the loading screen offers some hilarious commentary as well.
--> If you die by falling off a ledge, it's really just your own fault.
--> [[CaptainObvious When getting shot at, avoid bullets.]]
--> If you get stuck, you can always cheat by reading an online FAQ.
--> [[WhatCouldHaveBeen Picking up a turd in a toilet won't lower Ego, but we really wanted it to.]]
--> You can slap our wall boobs, since real girls don't like it when you slap theirs
* For the Interactive Achievement 2011 Awards, Gearbox made [[http://www.youtube.com/watch?v=-nVwNrH4gqQ this video. And it is HILARIOUS!]]
* Duke's gleeful cackle and mimicking of complex machinery winding down broken after he trashes a turbine in "Underground" was so unexpected yet appropriate, Also, he has never felt so good about vandalism before.
* Near the end of the game:
--> '''General Graves:''' America is counting on ''you''.
--> '''Duke:''' ''[singing]'' [[TeamAmericaWorldPolice America, fuck yeah!]]
* Punching out Christian Bale. Nothing more needs to be said.
* In The Doctor Who Cloned Me, [[spoiler: Dylan]] becomes one of the funniest parts of series. [[spoiler: His]] best line is [[spoiler: his]] idea to get Duke [[spoiler: to the moon]] (reverse the polarity) and the follow-up. It's just how proud [[spoiler:he]] sounds about the idea that sells it.
** Voice verification.
-->'''Duke Clone''': Repeat after me. I've got balls balls balls balls of steel.
-->'''Duke''': I've got balls...er...balls, balls, balls of steel?
----