* Remember the [[LethalJokeItem Hand Cannon]] from ''VideoGame/DeadSpace2''? It's back... as [[ThePowerOfRock the Devil Horns]]. It has infinite ammo and is basically a OneHitKO on anything. But, reload it, and Isaac will headbang.
* Isaac trying to use his stasis module to slow down the automated freeway traffic, which looks like a typical simple stasis puzzle from the previous two games. Except that he only slows down ONE transport, causing the rest to crash in a massive pileup.
--> '''Isaac''': ''(after a moment of StunnedSilence at the wreckage)'' [[{{Understatement}} Oops.]]
** Not helped by the fact that whether you're playing solo or co-op, using Stasis for this was Isaac's [[CallBack idea]].
* Isaac and Carver's reaction to the Hunters, which mirrors the player's.
--> '''Isaac''': ''(after narrowly escaping several regenerating Necromorphs)'' God, I hate those things....
* Late in the game while in the research facility in cooperative, before you enter the generator room to return power to [[DeadlyGas the sterilization gas]] to clear a bunch of infestation. Carver and Isaac get this little gem...
--> '''Carver:''' Wait, you're not thinking we should mess with this sterilization system, are you?\\
'''Isaac:''' ''(almost teasing tone)'' ''[[BlatantLies Mmmm]]''[[BlatantLies aybe?]]\\
'''Carver:''' Isaac, it's gas that eats your face!\\
'''Isaac:''' Come on, [[WhatCouldPossiblyGoWrong what's the worst that can happen]]?\\
'''Carver:''' It's ''gas'' that eats your ''face.''
** It's a bit funnier if you remember one of Isaac's quips from ''Dead Space 2''. [[BrickJoke He really is full of bad ideas!]]
* After the crash on Tau Volantis, Isaac searches desperately for Ellie amongst the wreckage in a snowstorm. As he enters a relatively intact section, he suddenly spies a corpse on the floor. He panics, rushes over and digs at the snow and ice. He then rolls the corpse over and...it isn't Ellie. It's another crew member, with half of his head is blown off. Isaac's response?
--> '''Isaac''': Oh, thank ''God''. ''(drops corpse back on the ground)''
** Better yet, the dead crewmember was Mark Rosen, who was a prick to Isaac just moments before. And after Isaac offered to help him, on top of that! No wonder he wasn't missed.
* Despite their generally nightmare inducing nature, one of the then-sentient feeders in an audio recording gets this bit of unexpected humor.
--> '''Feeder:''' Ackerman, is that you?\\
'''Ackerman:''' Oh, God...\\
'''Feeder:''' I smell you... I smell... FRESH MEAT!
* Danik's disbelief that Isaac could still be alive is priceless thanks to the delivery. Instead of the usual anger, confusion, or frustration, he's very... ''affable'' about it.
--> '''Danik:''' Isaac, is that you? You are ''unbelievably'' hard to kill, are you aware of that?
* Whenever Isaac meets Danik, Isaac ''always'' escapes.
** It should be noted that every time Isaac meets, or is a bout to meet, Danik, that Danik always boasts that he will have Isaac killed.
* After [[spoiler: reconstructing Rosetta]], there's a large info-dump that's intended to be dramatic and clear up most of the lingering questions about Convergence and "Make Us Whole." Except the delivery is Isaac sitting on the floor, monologuing like he's on a drug trip. And when he's finished, Isaac stands up to see Danik and his goons are standing there holding Carver and Ellie at gunpoint, having just listened to the entire thing without saying a word.
* Near the end of the game:
--> '''Carver:''' Congratulations, you just turned [[spoiler: the city]] into a pretzel. What now?
* When [[DoubleStandard Jacob Danik]] gets mad at Isaac for "tampering with things [he] knows nothing about." Isaac's response?
-->'''Isaac:''' [[ShutUpHannibal Right back at you, jackass.]]
* The Bot Personality Pack DLC for the Scavenger Bot turns your [[CuteMachines adorable bot]] into [[SnarkyNonHumanSidekick an incredibly snarky rover]] that has a complement of lines that occasionally goes toe-to-toe with [[VideoGame/KnightsOfTheOldRepublic HK-47's]] dark humor.
--> '''Scavenger Bot:''' Ah, there you are. A gentleman was looking for you. [[BodyHorror His face was on backwards. And he had]] ''[[BodyHorror bone]]'' [[BodyHorror swords for hands.]] I sent him your way. I hope you don't mind. He seemed anxious to find you.
--> '''Scavenger Bot:''' Nothing much to report, sir. I'll try looking over here. With any luck, it'll be more resources, and... ''fewer'' body parts.
--> '''Scavenger Bot:''' What do you do with all of these resources? If you're building weapons to chop even ''more'' limbs off people. I'll have no part of it. That makes me an accomplice, we're simply not that close.
* Despite the [[DarkerAndEdgier much, much darker tone]] of the DLC, Isaac and Carver still manage to engage in some humorous dialogue
--> '''Carver:''' Let's get looking, dead boy.\\
'''Isaac:''' [[DudeNotFunny That's not funny]].\\
'''Carver:''' Yeah it is.
** In ''Awakened'', Isaac admits [[GivingUpOnLogic what people probably had figured out]] by ''2''.
--->'''Isaac:''' I quit trying to make sense of it all back on the ''Ishimura''.
** Another line from ''Awakened'', after Issac has essentially hotwired a two-hundred year old spaceship's shockwave system:
---> '''Carver:''' Let's go, let's go!\\
'''Issac:''' We may just blow up.\\
'''Carver:''' We ''may'' just get saved by aliens again. Just hit the damn button.
* Most of the co-op banter between Isaac and Carver when they run into the giant drill. The end of that sequence has the crowning moment, though.
---> '''Isaac''': ''(catching his breath)'' Jesus... I thought that was it...\\
'''Carver''': ''(chuckles)'' Yeah... ''(chuckles again)'' [[PrecisionFStrike Fuck]] [[DeathWorld this planet]]. C'mon.
** Isaac has bad history with giant drills. It'd be shocking if this incident ''doesn't'' give him a serious aversion to them.
---> '''Isaac''': Ellie?! I'm trapped and the drill is trying to kill me!
** This gem from the teaser's co-op gameplay:
--->'''Isaac''': ''(after he and Carver temporarily halt the drill's careening around them)'' Now what?\\
'''Carver''': ''You're'' [[TheSmartGuy the rocket scientist]]; ''you tell me!''\\
'''Isaac''': ''(defensive)'' [[ImADoctorNotAPlaceholder Hey, I work on ships, not giant drills!]] Just--look for an override!\\
'''Carver''': What the ''fuck'' is that?!
* Starting a NewGamePlus means you get whatever weapon you were holding at the end of the game in place of the pistol Carver hands you. This can cause slightly ridiculous situations, like Carver tossing a civilian an assault rifle with an underslung grenade launcher, or complete absurdity, like Carver handing him a [[ChainsawGood Ripper]] that sets everything it touches on fire.
** Or the Devil Horns.
* In one of the optional (two-player-only) missions, you come across a child's banner for a birthday party. But instead of saying "Happy Birthday," it says, "[[DisappearedDad Daddy Hates Me]]." The surrealism of the message (including its cutesy font) achieves a kind of BlackComedy.
* When your co-op partner drops equipment from their inventory and depending on how they're standing, the items can just... fall from between their legs. This can seem really strange/hilarious if they drop a whole load of medium health packs that are the same size as cereal boxes.
* In one of the S.C.A.F. logs found:
--> '''Mahad:''' I want all of them! Every last bullet, private!\\
'''Caufman:''' I'm sorry General Mahad, sir, but without the munitions key —\\
'''Mahad:''' What's your name son?\\
'''Caufman:''' Uh, Caufman, sir! P-Pri-Private Tim Caufman, sir!\\
'''Mahad:''' Do you like potatoes, Tim?\\
'''Caufman:''' Y-Yes, sir. I... I... I guess so, sir.\\
'''Mahad:''' Good. Because if you don't find the goddamn key and have those munitions on a train by sundown, you'll be [[PeelingPotatoes peeling goddamn potatoes]] [[CoolAndUnusualPunishment for the rest of your goddamn miserable excuse for a military career]]!