* Pretty much whenever a let's player gets scared by this game. In fact, [[http://www.youtube.com/watch?NR=1&v=L1RKuM57nPA&feature=endscreen here's]] a compilation of reactions.
* [[http://www.youtube.com/watch?v=IHHhWyG-zYk&list=FLConvpVrr9mBnunMj19Sq6Q&index=1&feature=plpp The new add on must be seen to be believed.]]
* [[http://www.youtube.com/watch?v=V8k2neQVbko The Water Scene]] Made even more funny with the subtitles. A Let's Player being freaked out by water demons never sounded so hilarious.
* The [[GameMod Custom Story]] [[{{Troll}} Dark Room]]. Pretty much [[MemeticMutation Meme Mania]].
* No game has perfect AI, and the grunt's can be exploited to hilarious results, such as getting it to break down an open door, hiding behind a table in a corner, and piling up a bunch of stuff, then attracting its attention. That last one can occasionally cause it to smash it all, which then freezes it in search mode.
** Anytime someone trolls the monsters. Also, replace the chase music with [[http://www.youtube.com/watch?v=ZnHmskwqCCQ Yakety Sax]] and the monster sounds with the guy from Yelling At Cats.
* [[WesternAnimation/MyLittlePonyFriendshipIsMagic The Small Horse]] series of custom stories. Starts off with a [[OnePiece ship captain]] finding and bringing home an EldritchAbomination that looks like a little pink horse, with the help of a bald German guy, unwittingly unleashes a great evil by doing so, and must now fix his own mistake by embarking on an epic quest that involves lots of ponies, hilarious {{Jump Scare}}s, VideoGame/{{Pokemon}}, flying naked corpses with extendable necks, mining for boulders using the [[Franchise/MastersOfTheUniverse Master Sword]], LegendOfZelda-esque dungeons, [[VideoGame/TeamFortress2 engineers and sentries]], zombie Fluttershy, a crazy race track, the [[VideoGame/{{Portal 2}} Space Core]], [[Series/DoctorWho Daleks]], and a [[AnimatedArmor giant suit of armor]] that likes to jump out and yell "Hello!" when you least expect it..
* Once Daniel enters the Cistern, a flashback occurs.
-->'''Daniel''': You are conserving water from the spring?\\
'''Alexander''': Yes, it enables me to control the water in the drain sewers to some extent. Also, it can be used for all sorts of purposes.\\
'''Daniel''': [[ComicallyMissingThePoint Like for drinking?]]
* The "Killings in Altstadt" custom game has parts where the player can buy items from a room which is run by a Grunt with an ushanka hat speaking in a Russian accent.
* Though a very serious scene, NO ONE expected Daniel to say something as silly as, "[[SarcasmMode Please, I didn't do anything. mah mah mah,]]" while he was cutting a man to death in a ritual killing. BlackComedy at its finest until the horror hits you.
* In "Lost the Lights", you come across a lot of short notes. That are poorly written in English. Eventually, you come across across a storage area with a letter on a shelf. All it is says is "[[MemeticMutation Nope.]]"
** [[ItMakesSenseInContext "The Don't Go Down the Hole" Easter Egg.]]
** [[spoiler: "HI, BILLY MAYS HERE!" jump scare.]] What?