* ''Roleplay/EnemyQuest'' has the '''H'''uman '''V'''isitor '''A'''ffairs '''O'''ffice, or HVAO. Their job is to keep the peace between humanity and the alien Visitors, and to study the Visitor's culture and mannerisms.
* In ''Webcomic/FisheyePlacebo'', there is the '''B'''ureau of '''I'''nformation '''T'''echnology and '''C'''ivil '''H'''armony. Also known as the [[EmbarrassingNickname B.I.T.C.H.]]
* ''Webcomic/{{Fans}}'' adjusted the '''F'''ederal '''B'''ureau of '''I'''nvestigation into the '''F'''antasmagorical '''I'''nvestigation '''B'''ureau, or [[TheMenInBlack FIB]].
* ''Webcomic/{{EVIL}}'' stands for the '''E'''lite '''V'''illains '''I'''nstitute of '''L'''earning. It also features such organizations ass ASS ('''A'''cademic '''S'''uperiority '''S'''ociety) and FOWL ('''F'''uture '''O'''pressors of the '''W'''orld at '''L'''arge).
* The secret anti-alien organization in ''Webcomic/ItsWalky'' is called SEMME... which ''does'' stand for something, but the author has yet to reveal what. It's pronounced like "semi", creating useful pun fuel. In one strip, part of the E on their building is blown off, prompting Walky to start calling the organization "SEMMF".
** I'd think there'd be more pun fuel if it were pronounced [[{{Seme}} how it looks]].
** The author once claimed to have forgotten what SEMME stood for (having invented it in his childhood).
* On ''Webcomic/TheWotch'', one character guessed that the first two letters of the [[StrawFeminist radical feminist]] group D.O.L.L.Y. stood for "'''D'''aughters '''o'''f", but [[TheUnreveal the rest was never determined]] and the [[spoiler: leader was defeated just before she could reveal its full meaning - and [[TakenForGranite turned into a dolly as punishment]] ]].
* In a 1990s Penn State in-joke (because [=PepsiCo=] seemed to have a great deal of influence at the college), ''Webcomic/TheInexplicableAdventuresOfBob'' once had a [[{{Kaiju}} giant monster]] called the '''P'''eople-'''E'''ating '''P'''oly-'''S'''orbate '''I'''nsectoid.
* ''Webcomic/DeviantUniverse'' has R.A.P.T.O.R. ('''R'''esistance '''A'''gainst '''P'''eople '''T'''errorizing '''O'''ur '''R'''ace) and A.R.E.S. ('''A'''ggression '''R'''eigns '''E'''ver '''S'''upreme).
* ''WebComic/VampireCheerleaders''[=/=]''WebComic/ParanormalMysterySquad'':
** Stephanie chose the name, ''"'''P'''aranormal '''M'''ystery '''S'''quad"'' [[StealthPun because the initials signified]] [[http://www.vampirecheerleaders.net/strips-vc/no_guys_allowed that they were an all girl group]] of cryptid hunters. Get it?
** There's also PETM[[note]](pronounced "pet 'em")[[/note]], which stands for: '''P'''eople for the '''E'''thical '''T'''reatment of '''M'''onsters. As their name implies, they're a monster rights government agency. Except under Marcy's leadership, they end up being [[ObstructiveBureaucrat Obstructive Bureaucrats]], who're [[AnimalWrongsGroup more concerned with monster rights than public safety]] (as their name ''also'' implies, they're a TakeThat at PETA).
* ''Webcomic/TheKAMics'' had the Semi-Mystical Otherworldy Gates or S.M.O.G. Nothing deep about the name though, the author took the acronym from a running gag he was part of on one of [[http://nitcentral.philfarrand.com/discus/messages/17579/14907.html?1024222400 Nitcentral's humor threads]].
* Mr. Mighty in ''Webcomic/EverydayHeroes'' is a member of The '''S'''ociety of '''A'''mazing, '''V'''aliant, and '''E'''xtrememly '''U'''nselfish '''S'''uperheroes ... S.A.V.E.U.S.
** According to the cast page, Mr. Mighty has previously worked for the '''T'''eam '''H'''oping to '''U'''phold '''D'''ecency ('''THUD'''), the '''W'''hite '''H'''ouse '''A'''dvanced '''P'''ersonnel '''P'''rotective '''O'''ffice ('''WHAPPO'''), the '''A'''merican-'''M'''exican '''I'''nternational '''G'''roup '''O'''f '''S'''uperheroes ('''AMIGOS'''), and the '''G'''roup '''O'''f '''O'''fficial '''D'''o-'''G'''ooders '''U'''nited '''I'''n '''S'''uppressing '''E'''vil ('''GOOD GUISE.''').
** The villains have their own group, the '''M'''id-'''W'''estern '''A'''lliance of '''H'''ired '''A'''ssassins, '''H'''enchmen, '''A'''narchists, '''H'''ighly '''A'''nti-social '''H'''ackers, '''A'''nd '''H'''ideous '''A'''bominations ('''MWAHAHAHAHAHA''').
%%* This episode of ''[[http://www.webcomicsnation.com/eddurd/irregemit/series.php?view=single&ID=214889 WCI High]]'' spoofs [[ComicBook/{{Shazam}} Captain Marvel]] when Blossom Pearl invokes her "magic word".
* Spoofed in [[http://antiheroescomic.com/comic/187 this spoilerific]] entry for "anti-HEROES", a comic which seriously ripped off the art style of ''Webcomic/TheOrderOfTheStick''.
* ''Webcomic/SluggyFreelance''
** The ''''N'''otification of '''U'''nified '''K'''indness' '''E'''nvelopes as the ultimate weapon within the Dimension of Peace.
** Also, from [[http://www.sluggy.com/comics/archives/daily/110215 this strip]], we get '''R'''ecording '''E'''ditable '''C'''ontent '''A'''pplication '''P'''eripheral.
** And [[http://www.sluggy.com/comics/archives/daily/090908 before that]] we get 4U City's '''M'''aternal '''O'''bedience '''M'''aintenance '''M'''edicating '''Y'''-Frames and the '''C'''hem-Lab '''R'''eview and '''A'''ssignment of '''P'''ersonnel Riff teaches.
** {{Defied}}: In "Falling", Riff declares that the '''S'''ynthetic '''H'''ybrid '''O'''ptimal '''D'''estruction '''D'''igbot '''I'''nfantry '''E'''xoskeleton is not acronymable.
* [[http://blag.xkcd.com/2009/09/02/urinal-protocol-vulnerability/ xkcd]] kicks it up a notch with the International Choice of Urinal Protocol. [[FridgeBrilliance Think about that for a second.]]
** A straighter example is [[http://xkcd.com/917/ this strip]], where Douglas Hofstadter's autobiography reads, "'''I''''m '''S'''o '''M'''eta, '''E'''ven '''T'''his '''''A'''cronym''".
* ''Webcomic/PennyArcade'' gives us '''G'''amers '''A'''gainst '''E'''xercise, a theoretical support group founded by Gabe who support traditional, controller-based gaming. Especially funny on a shirt.
* ''The Perpetual Aquarium'' had the fish take the Comprehesive Assessment and Report of Progress, or CARP. This was lampshaded when one of the fish comments that basing a year's progress isn't CARP, it's C----, and, when interrupted, comments that he likes anagrams.
* The FasterThanLightTravel drive in the ''Webcomic/{{Freefall}}'' universe is called the '''D'''angerous '''A'''nd '''V'''ery '''E'''xpensive drive.
** The name was suggested by a reader after the writer decided he had to call it something so he might as well call it DAVE.
* The Trustworthy Room test from ''Webcomic/TowerOfGod'' requires to gather rooms and teammates, though with a high risk of losing ones room. So Ja Wangnan and Nia decide to do it the gentlemanly way: Operation S.I.R., '''S'''tay '''I'''n the '''R'''oom.
* ''Webcomic/IrregularWebcomic'' has the '''S'''ecret '''A'''ction '''Squa'''dron '''T'''eam of '''C'''ryptid '''H'''unters.
* In the ''Webcomic/JetDream'' RemixComic, the T-Girls represent UsefulNotes/{{NATO}}'s '''J'''oint '''E'''lite '''T'''eam, '''D'''ivision of '''R'''aiding-'''E'''xpert '''A'''eronautical '''M'''aidens ('''J.E.T. D.R.E.A.M.''', or usually just "Jet Dream.") Their "little sister" team is '''J'''unior '''E'''chelon, '''T'''een '''T'''-Girls, '''E'''lle-Boys, and '''E'''sprit-Girls ('''N'''atural!), or '''J.E.T. T.E.E.N.''' Both organizations must deal with the sinister schemes of '''Z'''oned '''E'''xtortion and '''R'''emoval, '''O'''ccidental ('''Z.E.R.O.''').
* ''Webcomic/{{Erfworld}}'' has Lord Stanley the Tool's elite hobgobwin knights, the '''K'''nights '''I'''n '''S'''tanley's '''S'''ervice. [[DontExplainTheJoke They are, of course, a reference to the band KISS;]] their "war paint" is the makeup KISS wears onstage, and their name is a reference to the old moral panic-driven legend that KISS stood for "Knights In Satan's Service" (which it didn't).
* The GIGAHERTZ, or '''G'''izmonic '''I'''nstitute '''G'''raphical '''A'''utomated '''H'''yper-'''E'''nhancement of '''R'''eality through '''T'''eleportation and, uh... '''Z'''oom, from ''Webcomic/TheWayOfTheMetagamer''.
** Responded to with the '''C'''layton '''F'''orrester '''V'''olumetrically '''D'''isplayed '''E'''ntertainment and '''W'''orld-'''T'''aking '''O'''ver '''D'''evice, or, uh... CFVDEWTOD (khfhvhdootodd?) for short.
* ''Webcomic/PennyArcade'' played with it:
--> '''General''': We call it the '''K'''inetic '''I'''ngestion '''R'''egulator '''B'''io-'''Y'''organism.
-->'''[[VideoGame/{{Kirby}} Meta Knight]]''': Yorganism?
--> '''General''': [[ShoehornedFirstLetter Y is kind of a tricky letter.]]
** They're also the TropeNamer for the GreaterInternetFuckwadTheory.
* ''Webcomic/{{PHD}}'' [[http://www.phdcomics.com/comics.php?f=1100 explained how to create those]].
* ''Webcomic/{{Precocious}} has '''T'''ime '''A'''dvancement '''D'''evice '''A'''ssociated '''W'''ith '''A''' '''R'''elative '''P'''osition. T.A.D.A.W.A.R.P.
** A [[http://www.precociouscomic.com/archive/comic/2016/02/14 comic-within-the-comic]] has Max worry about girls giving him gifts with C.O.O.T.E.E.S, '''C'''amera-'''O'''utfitted '''O'''bservational '''T'''rackers, '''E'''mbedded '''E'''spionage '''S'''ystems.[[note]]those last two words were cut off in the comic and are only known through WordOfGod[[/note]]
* ''Webcomic/{{Homestuck}}'' has Nepeta's planet, the '''L'''and '''O'''f '''L'''ittle '''C'''ubes '''A'''nd '''T'''ea. ('''[[ShoutOut LOL]][[WebOriginal/LOLCats CAT]]''')
** Jake English gets a '''L'''and '''O'''f '''M'''ounds '''A'''nd '''X'''enon. [[Film/WeekendAtBernies In case you're wondering...]]
** And then later, when Caliborn takes over the narritive and is retelling the story, he can't remember the name of Dave's planet, and so invents his own. What does he call it? Land of Someone's Handicraft I Took ([[StylisticSuck LOSHIT]]).
* The ''Webcomic/CrossoverWars'' had their DRACO (Dimensional Reality Arcane Crossover Organizer)
* ''Webcomic/ElGoonishShive'' being a Word Salad Title, it's no wonder that [[http://community.910cmx.com/index.php?showtopic=9749&st=20&p=392573&#entry392573 according to some fans]],
--> "EGS" really stands for "'''E'''xtremely '''G'''ratuitous '''S'''hapeshifting"
* ''Webcomic/SarahZero'' has the '''P'''rivacy '''I'''nvestigation '''S'''chadenfreude '''S'''ecret '''S'''ervice
* ''Webcomic/FreakingAwfulPuns'' has scared away some potential readers with its acronym.
* ''Webcomic/SkinHorse'':
** Played with.
---> '''Violet''': And you're U.N.I.T.Y.\\
'''Unity''': Yep!\\
'''Violet''': The '''U'''pgradeable '''N'''ecrotic '''I'''ndependent... what?\\
'''Unity''': We don't talk about my acronym.\\
'''Violet''': Sorry, did I touch a nerve?\\
'''Unity''': Well, you know. People died and stuff.\\
'''Violet''': People died... because of your acronym.\\
'''Unity''': [[NoodleIncident The committee meetings, man! It was law of the jungle!]]
** In a later arc, we meet G.O.D.O.T., an AI whose primary function is a military-grade backronym generator. It turns out [[spoiler: G.O.D.O.T. went mad trying to work out what U.N.I.T.Y. could stand for, which is presumably the point at which people died.]]
** It turns out that Unity came up with her own acronym, [[spoiler:which pissed off Godot to no end]]. It's something Sweetheart said: "[[spoiler:Unity's Nice, I Tell You]]."
* From [[http://www.wastedtalent.ca/ Wasted Talent]], Jam's suggestion for an [[http://www.wastedtalent.ca/comic/and-acronym-here-and-acronym-there agriculture program.]]
* BOOBS ('''B'''asic '''O'''rganizational and '''O'''perational '''B'''a'''S'''eband) of [[http://ask-boobs.tumblr.com Ask Boobs]], a Tumblr-based Tron webcomic. The origins of her name are explained in [[http://ask-boobs.tumblr.com/post/17520242497/as-for-the-other-question-for-the-code-of-me this comic]]. She's, er...appropriately named.
* ''Webcomic/DorkTower'' has a TakeThat to Creator/MichaelBay, advertising that people should use the hashtag [[http://www.dorktower.com/2012/03/28/tmntwtf-dork-tower-28-03-12 #TAINT]] (Teenage Alien Imposter Ninja Turtles) for his TMNT movie.
** ''Dork Tower'' also has the group Matt started called '''G'''amers '''F'''or '''P'''ersonal '''H'''ygiene. At first Igor questions this acronym since "it doesn't spell anything," but then a very smelly geek walks by and he makes the sound effect "'''GFPH'''!"
* In [[http://www.captainsnes.com/2012/04/13/770-bunch-of-dhopes/ this]] ''Webcomic/CaptainSNES'' comic, we discover that [[VideoGame/ChronoTrigger Dalton]] is recruiting [[GoldfishPoopGang villains]] from different video worlds. His name for the team? The '''D'''altonian '''H'''yper-'''O'''ceanic '''P'''iratical '''E'''xtortion '''S'''quad.
* ''Webcomic/ThePackrat'' has the '''M'''emorymoog '''A'''coustic '''D'''eath Ray, a DeathRay [[ExactlyWhatItSaysOnTheTin powered by a Memorymoog polyphonic synthesizer]].
* ''Webcomic/WhiteDarkLife'' has the '''B'''oyfriend '''I'''nvented for '''L'''onely '''L'''adies, Naturally the character is named BILL.
* ''Webcomic/BrunoTheBandit'' once started a [[http://www.brunothebandit.com/d/19990615.html unicorn rights group]] which fit this trope.
* Paul in ''Webcomic/WeenieLicked'' calls Rosey '''C'''rony '''o'''f '''C'''uteness, or "COC".
* From ''Webcomic/SchlockMercenary'':
** From the eyetree quest storyline, there are the two Bradicor males '''R'''eally '''O'''ld '''D'''ude, or Rod, and '''V'''ery '''O'''ld '''G'''uy, or Vog, both of whom are millions of years old.
** Lt. Flinders and Enesby were ordered to come up with a manual of operations for the Toughs to standardize actions. [[http://www.schlockmercenary.com/2015-11-30 This strip]] shows the results: '''T'''oughs '''H'''andbrain '''O'''f '''O'''perations: '''S'''enior '''O'''fficers, or THOOSO; '''T'''oughs '''H'''andbrain '''O'''f '''O'''perations: '''P'''latoon '''O'''fficers, or THOOPO; '''T'''oughs '''H'''andbrain '''O'''f '''O'''perations: '''G'''runts, or THUG (treating the double O as a U, not letting language stand in the way of a memorable acronym); and the master volume that combines the previous volumes into one work: '''T'''oughs '''H'''andbrain '''O'''f '''O'''perations: '''M'''aster.
--->'''Enesby:''' THOOM, if you will.\\
'''Comodore Tagon:''' Well played. I think we '''all''' will.
* Webcomic/{{Clockwork}}: Mercia Fortress's RobotBuddy prototype is called '''M'''ercian '''A'''rtificial '''I'''ntelligence: '''L'''abour (Build '''0'''), shortening conveniently to MAILO, pronounced like "Milo".
* In ''Hoofstuck'', Rainbow Dash gets the '''L'''and '''O'''f '''Y'''outh '''A'''nd '''L'''onging.
* In WebComic/OzyAndMillie, Millie [[SoapboxSadie tries to protest]] against apathy, but [[{{Irony}} loses interest]] in making the sign and uses one that says "Down with apat". This draws attention from the '''A'''ccociation for '''P'''erfectly '''A'''rranged '''T'''ablecloths.
* In the ''Webcomic/{{Narbonic}}'' Sunday strips, there was [[http://narbonic.com/comic/renaming-dave-barker/ a competition]] to give [[SitcomArchNemesis MIT Student Dave Barker]] a new title, now that he had graduated and become a transport planner. The [[http://narbonic.com/comic/dave-barker-s-new-title/ winner]] was '''N'''ewly '''E'''ducated '''M'''aster of '''E'''xpressways, '''S'''ideroads and '''I'''nnercity '''S'''treets. Or "Helen's NEMESIS" for short.
* In ''Webcomic/{{Skyvein}}'', VISN is one of the many acronyms (short for the '''V'''isual '''I'''nformation and '''S'''urveillance '''N'''etwork).