* ''WebAnimation/HomestarRunner'':
** In the WebAnimation/StrongBadEmail "coloring", Marzipan teaches a kindergarten-like, [[PoliticalOvercorrectness unsettlingly politically correct]] learning program where students are called "life-blossoms". The program is called L.U.R.N., which stands for '''L'''ife-Blossoms '''U'''ndergoing '''R'''e-programming '''N'''aturally.
** The [=SBEmail=] "space program" introduces Strong Badia's space program, SBASAF (pronounced something like "sbace-aff"): The '''S'''trong '''B'''adian '''A'''dministration of '''S'''ome '''A'''luminum '''F'''oil.
** Paroded in the ''WebAnimation/CheatCommandos'' cartoon "2 Part Episode Part 1", where Flashfight appears on behalf of BEEF, the '''B'''ureau of '''E'''lite '''F'''ighting '''F'''orces. When Silent Rip points out that it spells "BEFF," Flashfight changed it to '''B'''ureau of '''E'''very '''E'''lite '''F'''ighting '''F'''orce. When Silent Rip pronounces it "Beef-fuh," Flashfight fires him.
** In a promo for ''VideoGame/StrongBadsCoolGameForAttractivePeople'', Strong Bad drops the name of the game, then adds "Or if you want to get in on the ground floor of the cool abbreviations: [=SBCG4AP=]!"
** A DVD-exclusive short, originally made for the 2006 Flashfoward conference, had Strong Bad introducing the "2002 MMOLOTRIPOIPARPG Conference" - which most likely stands for Massively Multiplayer Online Franchise/LordOfTheRings Initial Public Offering International Phonetic Alphabet[[note]]or possibly India Pale Ale[[/note]] Role Playing Game.
* Parodied in ''Bonus Stage'' with the robot MALCOLM: '''M'''achine '''A'''utomated '''L'''eadership ... '''COLM'''.
* ''WebAnimation/DarkSecretsOfGarrysMod'': Magyar Lénykutató Portál (Hungarian Creature Research Portal) is a foundation shortened as [[Toys/MyLittlePony MLP]] where scientists do research on pony related creatures.
* ''WebAnimation/DeclineOfVideoGaming'' shows the three protagonists invited to e3 (the Electronically Exaggerated Entertainment Show) by the '''G'''aming '''O'''rdinators '''N'''ational '''A'''ssociation of '''D'''eveloping '''S'''ystems.
* TOME centers around a MMORPG universe called the '''T'''errain '''O'''f '''M'''agical '''E'''xpertise.
* ''WebAnimation/NapsterBad'' includes an anti-piracy organization called the '''R'''ecording '''A'''ssociation for '''P'''opsong '''E'''conomics.
* ''WebAnimation/TheMostPopularGirlsInSchool'': What the acronym on Brittnay's Revenge Campaign read as (note the bold letters): '''M'''atthews '''P'''lan to '''G'''ain '''I'''nfluence '''S'''ubvert '''S'''hitty '''E'''gomaniacs '''A'''nd '''S'''eek '''O'''ut '''N'''ew '''F'''orms '''O'''f '''U'''nholy '''R'''etribution.
** There's more in the rest of the Website/IndieGogo videos. They all spell the same thing-'''MPGIS SEASON FOUR'''.
** [[UnfortunateNames TWAT]]: '''T'''elevision '''F'''or '''W'''omen '''&''' '''T'''weens
*** Also funny, is the 'A' part in TWAT is actually 'And'. Yet when it's laid out, it's presented as an ampersand.
* The [=DSBT=] of ''WebAnimation/DSBTInsaniT'' stands for '''D'''yl'''S'''chool'''B'''lind'''T'''ure, which makes no sense unless you watch [[AllThereInTheManual Special Info Episode]].
* ''WebAnimation/{{RWBY}}'': Students are put into teams of four with the team name being based on the names of the four members, the first name corresponding to the team's leader.
** Team RWBY (pronounced "Ruby"): '''R'''uby Rose, '''W'''eiss Schnee, '''B'''lake Belladonna, '''Y'''ang Xiao Long. Each member is based on a character from a fairy tale.
** Team JNPR (pronounced "Juniper"): '''J'''aune Arc, '''N'''ora Valkyrie, '''P'''yrrha Nikos, Lie '''R'''en. Each member is based on a historical/mythological figure who at one point disguised themselves as the opposite gender, and is the gender they disguised themselves. For example, Jaune is a male UsefulNotes/JoanOfArc.
** Team CRDL (pronounced "Cardinal"): '''C'''ardin Winchester, '''R'''ussel Thrush, '''D'''ove Bronzewing, Sky '''L'''ark. The team is both bird and Christian themed, as is the word "cardinal".
** Team CFVY (pronounced "Coffee"): '''C'''oco Adel, '''F'''ox Alistair, '''V'''elvet Scarlatina, '''Y'''atsuhashi Daichi. The team is based both off the color brown and various desserts.
** Team SSSN (pronounced "Sun"): '''S'''un Wukong, '''S'''age Ayana, '''S'''carlet David, '''N'''eptune Vasilias. Similar to JNPR, each member is based on a character from a classic tale, but no cross-dressing is involved this time.
** When the teams get mixed up a bit, Team RNJR (pronounced "Ranger") is formed: '''R'''uby Rose, '''N'''ora Valkyrie, '''J'''aune Arc, Lie '''R'''en. There is some argument on the name and who is technically the leader--Ren thinks Ruby is the leader since they're following her mission, Nora thinks Jaune is the leader since there are more of them. Her suggestion was team JNRR (pronounced "Junior") -- '''J'''aune Arc, '''N'''ora Valkyrie, Lie '''R'''en and '''R'''uby Rose -- but after Jaune says the name out loud, he sighs and says RNJR just sounds better.
** A generation prior to the present day, Team STRQ (pronounced "Stark") consisted of: '''S'''ummer Rose, '''T'''aiyang Xiao Long, '''R'''aven Branwen, '''Q'''row Branwen.
* PlayedForLaughs in one ''WebAnimation/RedVsBlue'' PSA about trigger warnings, where Doc has Simmons create two systems for detecting potentially harmful content and finding other people's triggers. Said systems are called the Rapid Anti-Provocation Emergency System and the Cautionary Ubiquitous Neurological Trauma Scanner, respectively, much to Doc's chagrin.
* In ''WebVideo/UltraFastPony'', Twilight Sparkle insists that [[UsefulNotes/ObsessiveCompulsiveDisorder Obsessive-Compulsive Disorder]] should instead be known as ABCD: Aggressively Belligerent Compulsive Disorder.
* In ''WebAnimation/XRayAndVav'', The Mad King seeks to empower himself by using the '''E'''nergized '''D'''airy '''G'''athering '''A'''bsorption '''R'''ig.
* In the animated series for the game "VideoGame/HappyWheels", the five main characters form the '''D'''efensive '''E'''ducation '''A'''wareness '''T'''eam and '''H'''ealth '''S'''ociety, or D.E.A.T.H.S.
* ''WebAnimation/BattleForDreamIsland'' loves doing these.
** Most commonly, the [=TLC=] that eliminated contestants go to stands for the '''T'''iny '''L'''oser '''C'''hamber.
** In Episode 18 of the first season, it is shown that the recommended characters that did not make it into the contest go to the [=LOL=], or the '''L'''ocker '''o'''f '''L'''osers. In Season 2, the aforementioned [=TLC=] is stored ''inside'' the [=LOL=].
** In [[WebAnimation/BattleForBFDI Season 4]], eliminated contestants go to an eternal algebra class held in "Eternal Algebra Class Withfour"... though it turns out Four isn't good at acronyms, so it is instead written out as [[ShoehornedFirstLetter "Eternal Xalgebra Iclass Twithfour"]] to spell "EXIT".
** One of particular note, [[ShowWithinAShow Blocky’s Funny Doings International]], which just happens to share an acronym with the series itself.
** BFDIA also has one of the teams named the Beautiful Face Devotion Institute (for one episode, but still), which also shares an acronym with the series. Another team is called the W.O.A.H. Bunch, which can either stand for "[[IronicName Wheel Ooze a Hole Bunch]]" or "Water's Open Aperture Hater Bunch".
** One of the teams in the fourth season is named Death P.A.C.T., which may sound like they have a huge impact on death, but actually stands for “[[IronicName Death Prevention And Creating Trust]]”.
** It happens again in BFB 14, where [[spoiler: Team Ice Cube finds a "Buried Forest, Deciduosly Insulated", also with the same acronym as the show.]]
** In post-split BFB, the eliminated contestants would go to the [=BRB=], also known as the '''B'''ig '''R'''otating '''B'''uilding.
** {{Subverted|Trope}} in episode 28 of BFB.[[note]]Also played straight with the episode's title: "B.F.B. = Back From Beginning".[[/note]] After the Announcer flings Purple Face away, Gelatin asks him where he sent him to, and the Announcer tells him "I.D.K.". That isn't actually an acronym, he just didn't know where he flinged him to.
* ''WebAnimation/InanimateInsanity'' has its eliminated contestants go to '''I'''diotic '''I'''sland when they are eliminated in the first season... which happens to be the show's acronym, too. {{Subverted|Trope}} in Season 2, where they go to Hotel OJ, but then {{Double Subver|sion}}ted in [[WebAnimation/InanimateInsanityInvitational Season 3]], where they go to '''I'''ndefinite '''I'''sland.
* When he is asked if ''{{WebAnimation/ONE}}'' is meant to be an acronym, Airy says that it is, but he is interrupted before he can say what it's meant to stand for, and we never actually find that out.
* ''WebAnimation/HelluvaBoss'' is about an [[MurderInc assassin business]] run primarily by imps called I.M.P. ('''I'''mmediate '''M'''urder '''P'''rofessionals).
* ''WebAnimation/MetaRunner'': [[spoiler: Tari’s name is derived from the AI that merged with half of Lucinia’s mind to create her: '''T'''urbo '''A'''rtificial '''R'''apid '''I'''ntelligence.]]
* ''WebAnimation/VeryBadChildren'': Rhoda Killjoy works for the D.O.S.D.R.G.M. or the '''D'''epartment of '''O'''verbearing '''S'''ocial Workers '''D'''edicated To The '''R'''emoval of Juveniles From '''G'''uardians Who Lost Their '''M'''arbles Inc.
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