* ''Manga/BlackLagoon'':
** SociopathicHero Revy as ''very'' choleric
** WideEyedIdealist Rock as melancholic
** BaldBlackLeaderGuy Dutch as phlegmatic
** MissionControl Benny as sanguine
* The original ''BubblegumCrisis'':
** Hot-blooded badass Priss (choleric)
** Stoic leader Sylia (melancholic)
** Linna the average Jane (phlegmatic)
** Perky and ditzy Nene (sanguine).
* ''ChronoCrusade'':
** Sanguine: [[NunTooHoly Rosette]] (HotBlooded, cares a lot about others and has a violent temper).
** Choleric: [[MsFanservice Satella]] (prideful, determined, and focused on her revenge).
** Melancholic: [[HeroicAlbino Azmaria]] (a prodigy singer, very caring about others, but insecure and hard on herself).
** Phlegmatic: [[FailureKnight Chrono]] (compassionate and nurturing, but easily discouraged)
* ''Anime/CowboyBebop'': The Bebop crew fits perfectly, with even the colors of their hair (or clothes in Faye's case) signifying their humors:
** Space-case Ed with bright red hair (sanguine)
** Hot-tempered Faye dressed all in yellow (choleric)
** High-strung Jet with black hair and dark blue clothes (phlegmatic)
** Brooding green-haired Spike (melancholic through and through).
* The four Dark Masters in ''Digimon Adventure'' fit this trope:
** The leading element Piedmon (choleric)
** The cold and calculating EvilGenius Machinedramon (melancholic)
** Pragmatic Metalseadramon (phlegmatic) who's a good boss towards his minions as long as they don't fail him
** Puppetmon, with his HotBlooded PsychopathicManchild demeanor (sanguine).
* ''Anime/DigimonTamers'':
** ArrogantKungfuGuy Rika (choleric)
** ControlFreak Henry (melancholic)
** TheHeart Takato (phlegmatic)
** StepfordSmiler Jeri (sanguine).
* ''Anime/DigimonSavers'' has two sets:
** The main cast:
*** HotBlooded Marcus (choleric)
*** TeenGenius Thomas (melancholic)
*** FauxActionGirl Yoshi (phlegmatic)
*** WildChild Keenan (sanguine)
** The original expedition:
*** GeniusBruiser Spencer (choleric)
*** BigBad Kurata (melancholic)
*** CoolOldGuy Yushima (sanguine)
*** [[GoodParents Good Parent]] Criers (phlegmatic).
* ''MagicalGirlLyricalNanoha [=StrikerS=]''. Natural leader (former) {{Tsundere}} Teana (choleric), knight-in-training Erio (melancholic), Caro (phlegmatic), and GenkiGirl [[ThePollyanna Pollyanna]] Subaru (sanguine). The last two's personalities actually caused a bit of family drama for their also melancholic adopted mother in the second ''[[AudioAdaptation StrikerS Sound Stage]]'', since [[NonverbalMiscommunication their combined introvertedness caused some communication problems]].
* Although everyone in ''OokikuFurikabutte'' gets his/her turn to shine, the story tends to focus on four core members of the baseball team:
** TheCaptain Hanai (choleric)
** TheStrategist Abe (melancholic)
** FragileFlower Mihashi (phlegmatic)
** CloudCuckoolander Tajima (sanguine).
* RurouniKenshin: Kenshin (melancholic), Kaoru (choleric), Sanoskue (sanguine), Megumi (phlegmatic), and Yahiko (leukine)
* ''Anime/{{Superbook}}'' (second season): Christopher Peepers (choleric), Joy (melancholic), Uriah (sanguine), and Gizmo (phlegmatic).
* The four main characters from ''{{Texhnolyze}}'': [[HotScientist Doc]] is Sanguine, [[OnlySaneMan Onishi]] is Choleric, [[TheStoic Ichise]] is Phlegmatic and [[WaifProphet Ran]] is Melancholic.
* ''Manga/TheLegendOfZelda:
** Four Swords Plus'' manga by Akira Himekawa takes a literal spin with this trope by dividing Link into four different entities with personality traits that reflect the color tunic each is wearing: Green is choleric, Red is sanguine, Blue is melancholic and Purple is phlegmatic.
** The [[http://www.newgrounds.com/portal/view/328825 flash animations by Hadouken Dude]] took this to absolutely ''hilarious'' levels, with Green Link as the melancholic (loyal/dedicated/uptight) one, Blue Link being the phlegmatic (analytical/sarcastic/smart) one, Red Link as the choleric (severe testosterone imbalance/lack of foresight) one, and Purple Link being the sanguine (along for the ride/accused of being gay because of personality and color) type.
* ''[[LightNovel/HaruhiSuzumiya The Melancholy Of Haruhi Suzumiya]]'': Haruhi (choleric, despite the title), Koizumi (phlegmatic), Yuki (leukine), Mikuru (phlegmatic and sanguine) and Kyon (melancholic). Tsuruya, [[TheSixthRanger an honorary member of the Brigade]], fills the full sanguine position.
* ''Anime/NeonGenesisEvangelion''
** Shinji Ikari (Phlegmatic)
** Asuka Langley Soryu (Choleric)
** Rei Ayanami (Melancholic)
** Toji Suzuhara (Sanguine)
** Kaworu Nagisa (Leukine)
* ''Anime/RebuildOfEvangelion''
** Shinji Ikari (Phlegmatic)
** Asuka Shikinami Langley (Choleric)
** Rei Ayanami (Melancholic)
** Mari Illustrious Makinami (Sanguine)
** Kaworu Nagisa (Leukine)
* Five-temperament ensemble in ''TokyoMewMew'': Ichigo, although [[IJustWantToBeNormal not happy about leading]] at first, accepting her responsibility later on (choleric); Minto (melancholic), Retasu (phlegmatic), the GenkiGirl Bu-ling (sanguine), and Zakuro (leukine).
* Anime/TheTowerOfDruaga:
** Jil's Group: Jil (melancholic), Ahmey (phlegmatic), Melt (choleric), Coopa (sanguine), and Kaaya (leukine)
** Neeba's Group: Neeba (melancholic), Fatina (choleric), Kally (phlegmatic), and Utu (sanguine)
* ''{{Trigun}}'' more often averts this trope in favor of the [[TheDrifter Lone Hero]] or the PowerTrio. Adding Wolfwood to the cast has a subtractive effect instead, generating a [[RedOniBlueOni duo dynamic]] that leaves Meryl and Millie on the sidelines. When they participate equally they fit the four temperaments: Meryl (choleric), Vash (melancholic, though pretending to be sanguine), Wolfwood (phlegmatic), and Millie (sanguine).
* ''TsubasaReservoirChronicle'': Kurogane (choleric), Syaoran (melancholic/phlegmatic), Sakura and Fai (sanguine/phlegmatic) and Mokona (sanguine).
* The four aunts in ''VisualNovel/UminekoNoNakuKoroNi'': Eva (choleric), Natsuhi (melancholic), Kyrie (phlegmatic), and Rosa (sanguine). Probably works with the uncles and cousins too. There are two different ways to categorize the [[VisualNovel/HigurashiNoNakuKoroNi Higurashi]] girls. The first is -- though actually works well no matter who is put in choleric position -- Shion (choleric), Rika (melancholic), Satoko (phlegmatic), and Rena (sanguine). Alternately, if you wanted to include Keiichi, the pattern shifts to Keiichi (choleric), Rika (melancholic), Mion - phlegmatic, Rena (sanguine), and Satoko - leukine. Alternately, if you wanted to just do the girls, just [[spoiler:replace Keiichi with Shion]].
* In the original ''WeissKreuz'': Aya (choleric), Omi (melancholic), Youji (phlegmatic), and Ken (sanguine).
* In ''WolfsRain'': Tsume (choleric), Kiba (melancholic), Toboe (phlegmatic), and Hige (sanguine).
* ''Anime/YesPrettyCure5'' tries to throw us off by having four temperaments among five characters--both Nozomi and Urara are sanguine. Rin is choleric, Komachi is phlegmatic (though not lazy by any stretch), and Karen is melancholic.
** In ''Anime/FreshPrettyCure'': Love is sanguine, Miki is choleric, Inori is more likely Phlegmatic, and Setsuna is a mix of melancholic and leukine.
** In ''Anime/HeartcatchPrettyCure'', [[ShrinkingViolet Tsubomi]] is phlegmatic, [[GenkiGirl Erika]] is sanguine, [[StudentCouncilPresident Itsuki]] is Leukine, and [[BrokenBird Yuri]] is melancholic.
** This dynamic also returns in ''Anime/SmilePrettyCure''. Miyuki is sanguine, Both Akane and Nao are choleric, Yayoi is phlegmatic, and Reika is melancholic.
* ''Manga/{{Voltron}}'':
** Boys: Keith (Leukine), Lance (Choleric), Hunk (Phlegmatic), Pidge (Sanguine), Sven (Melancholic).
** Girls: Allura (Sanguine), Romelle (Melancholic)
* The main four of ''Manga/YuYuHakusho'': Yusuke (melancholic), Hiei (choleric), Kurama (phlegmatic), Kuwabara (sanguine) and Botan (leukine).
* ''Anime/YuGiOh'': Yugi/Yami (melancholic), Tea (phlegmatic), Joey (choleric), Tristan (sanguine), and Solomon (leukine).
* ''Anime/YuGiOh5Ds'': Jack (choleric), Yusei (melancholic), Akiza (phlegmatic), and Crow (sanguine).
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