!!The Middleman
The titular character, he's a LegacyCharacter who fights comic book type monsters and {{Mad Scientist}}s with an array of gadgetry and good old fashioned fisticuffs. Works for [[HeroesRUs O2STK]], the Organization 2 Secret To Know.
* AllergicToLove: A mild case.
* AlliterativeName: [[spoiler: Clarence Colton]].
%%* BadassInANiceSuit
%%* BadassNormal
* BigBrotherInstinct: Stated himself that thinks of Wendy like a little sister.
* DiscoDan: He acts like a GoldenAge square jawed super hero.
%%* GenreSavvy
%%* GoshdangItToHeck
%%* NoNameGiven
* StarCrossedLovers: With Lacey.

!!Wendy Watson
The POV character, she was a temp working at [[FunWithAcronyms A.N.D. Labratories]] when a genetic abomination reminiscent of a {{hentai}} tentacle monster attacked her and the Middleman stepped in. Now she's his apprentice/sidekick/partner.
* ActionGirl: After her training with Sensei Ping.
%%* AlliterativeName
* BirdsOfAFeather: With Tyler.
%%* BrainyBrunette
%%* DeadpanSnarker
%%* DisappearedDad
%%* GenreSavvy
* IHaveManyNames: Lacey calls her "Dub-Dub" while the Middleman calls her "Dubbie." Tyler calls her "Dubs" and Noser always uses her full name. Ida uses a variety of sarcastic and/or insulting nicknames.
* JumpedAtTheCall: Her reaction to the knowledge that ghosts exist? "Oh cool!"
** "Cool!" is also Wendy's reaction to Zombies. And Vampires, and... you get the point.
** RefusedTheCall: At first. But it was mostly due to The Middleman and Ida framing her for the destruction of her last workplace that freaked her out. But then Ben decided to break-up with her and film it and the rest is history.
** Her accepting the giant monster's existence and leaping at it with a letter opener instead or running and screaming is what got her the job in the first place. She jumped at the call before there was even a call.
%%* MartialPacifist: Parodied.
* OrphansPlotTrinket: Her dad's lighter (though she's technically not an orphan).
%%* OtakuSurrogate
* SpicyLatina: Invoked by Middleman '69, who refers to Wendy as "a hot and spicy Spanish girl."
* TechnicalPacifist: Wendy hates guns, but has a bit of a geeky affection for the sci-fi weapons the Middle organization uses. She also loves training with Sensei Ping, and she frequently protests the Middleman's tendencies toward violence. But she had no problem whatsoever beating a venomous Peruvian Pike to death.
* UnfazedEveryman: Her reaction to the lab explosion is how she got the job.
* YouNeedToGetLaid: Well, at least Lacey thinks so.

The Middleman's robot assistant stuck in the form of a snarky middle-aged schoolmarm. Is constantly saying that Wendy Watson is a stoner.
%%* AIIsACrapshoot
%%* DeadpanSnarker
* DisneyDeath: The one from the series' first half actually does die, but [=O2STK=] promptly sends an identical replacement.
%%* {{Jerkass}}
%%* RidiculouslyHumanRobot

!!Lacey Thornfield
Wendy's roommate in their illegal sublet, she's an animal rights activist as well as another young, photogenic artist. She's also got the hots for the Middleman.
* GranolaGirl: A fairly sympathetic example.
* MissingMom: Dr. Barbara Thornfield, MD, PHD.
* StarCrossedLovers: With the Middleman, but she has no idea why.
* StuffedIntoTheFridge: [[spoiler:In the unaired 13th episode and graphic novel 'Doomsday Armageddon Apocalypse', Lacey is in the final stages of T-cell polymphocytic leukemia. Manservant Neville chose to punish the Middleman by giving Lacey this disease because it is the same disease which claimed the life of the other woman the Middleman loves, his mentor-and-prior Middlewoman Raveena Rao.]]
* TwoLinesNoWaiting: Usually there's a A-plot with MM and WW and a B-plot with Lacey and Noser/Pip/someone else. Sometimes they converge.

Another tenant in Wendy's building, he usually stands out in the hall with his guitar and speaks [[WaxingLyrical mostly in pop culture references]]. [[InformedAbility We never actually see him play.]]
%%* {{Cloudcuckoolander}}
* TheDreaded: Noser's talent and reputation as a ventriloquist are so great that the mere mention of his name causes one contestant in a competition to drop out, leave and quite possibly quit competing all together.
* InformedAbility: Not only his guitar playing skills, but his skills as a ventriloquist.
* OldShame: His childhood career as a ventriloquist.
%%* OnlyOneName
* ThePiratesWhoDontDoAnything: Noser spends an awful lot of time not playing the guitar. He also has an incidental sideline in not riding a motorcycle, and an entire episode built around his not being a ventriloquist.

!!Roxy Wasserman
A reformed succubus who works as a fashion designer and fronts a modeling agency, she helps MM with mystical problems.
* DarkIsNotEvil: The whole point of her fashion agency actually. It gives Succubi and Incubi a place to work and get reformed like she did.
%%* ForHalloweenIAmGoingAsMyself

!!Sensei Ping
Trainer for both the Middleman and Wendy. Possibly awesome incarnate. Knows the [[TouchOfDeath Wu-Han Thumb of Death]].
%%* BadassNormal
* HeWhoMustNotBeSeen: Though mentioned a lot, he's seen in a total of one episode.
%%* JerkAss
%%* LargeHam: He's played by Mark Dacascos. Nuff said.%%Zero-context example, Word Cruft.
%%* MickeyMousing: His ''every move''.%%Zero-context example.
%%* OlderThanTheyLook
%%* ThirdPersonPerson

!!Tyler Ford
A prior candidate for the Middleman's apprentice, instead he met Wendy, forgot her with [[EasyAmnesia two day amnesia]], then met her again and became her boyfriend.
* EasyAmnesia: Knocked on the head once and has two day amnesia. Yeah, just go with it.
* MissedTheCall: His roommate mislaid his job offer letter.
%%* ThePeteBest

!!Interrodroid 3000 through 7000
A training robot that helps around the Middleman HQ.
* TheChewToy: It tends to get its head ripped off. A lot.

Son of the owner of the apartment building Wendy and Lacey live in, he's a really big {{jerkass}}.
* BadassPreacher: In the Mirror-Middleverse.
* ContemplateOurNavels: His one man act was called "Hey, Mr. God, What About Me?"
* JerkAss: A really, really big one, and a plagiarist on top of that.

!!Joe 90
Another resident of Wendy's and Lacey's apartment, a failed law school student who makes very phallic sculptures.

!!Manservant Neville
The CEO of FATBOY Industries, which makes everything from coffee to ice cream to the uMaster, a virtually featureless cube that just happens to be ''the'' cool gadget that every young, creative artistic type just has to have.
* AGodAmI: The Doomsday Armageddon Apocalypse.
* BlandNameProduct: The uMaster, which is treated as a synthesis of every cool i-gadget Apple has ever made (but only to the extent that everyone has to have one, as we are never shown what, if anything, the uMaster actually does.)
* CorruptCorporateExecutive: Maybe. In the altUniverse, he was good, so he might be evil in this universe. Confirmed by the Doomsday Armageddon Apocalypse. He ends up very nearly taking over the world.
* EvilIsHammy: Mark Sheppard has ''way'' too much fun at the live reading, even overacting his death.
* InkSuitActor: The comic incarnation of the character was very different to the TV version, but was deliberately drawn to resemble Creator/MarkSheppard long before the chance to actually cast him came along.
%%* ItIsPronouncedTroPAY
* WellIntentionedExtremist: His plan to [[spoiler: become a Living God]] is environmentally motivated.

Wendy's boyfriend of over a year, breaks up with her for a film school project.
* {{Jerkass}}: Granted he feels terrible about the whole "breaking up with Wendy for a project" thing at first, he still did something so selfish in the first place and later posted it online.
* TrueArtIsAngsty: [[invoked]] The reason he breaks up with Wendy; his professor didn't think he had enough pain in his life.