[[folder:Main Characters]]
!!!Peter Parker/Spider-Man
Bitten by a radioactive spider on a school field trip, young Peter Parker attempted to cash in on his new powers, and signed up for a pro wrestling contest, and pinned the champion. When the promoter cheated him out of his winnings, Peter stood by as a thief robbed the promoter.

The same thief later killed his Uncle. Peter began using his newfound powers responsibly and began fighting crime. One of the first criminals he faced was The Green Goblin. When The Goblin learned that it was Peter under the mask, he attacked his Aunt May, and kidnapped the girl he loved, Mary Jane. After a dramatic battle, The Green Goblin revealed himself to be Norman Osborn. Seconds later, The Goblin was killed when he accidentally impaled himself on his glider.

He later began studying at ESU, along with his dream girl Mary Jane and his best friend Harry, the son of The Green Goblin. Harry isnít aware that his Dad was The Green Goblin, and hates Spider-Man, believing he murdered him. Peter makes his living by selling pictures of Spider-Man to J. Jonah Jameson. After being ripped off one too many times, he decided to start selling video footage to Empire1, where he met Indy, a research assistant who took a great shining to Peter.

Peter is still in love with Mary Jane, but feels that being with her would only jeopardise her safety again, which often leads to awkward feelings between the two of them, especially as he recently began seeing Indy [[spoiler: before he was tricked into throwing her off a building by the Gaines twins]].

[[spoiler: Vowing never to let Spider-Man hurt anyone else, Peter threw his Spider-Man costume into the river and stopped being Spider-Man]].

* BadassBookworm
* BewareTheNiceOnes: Kraven found this out the hard way.
* ComesGreatResponsibility
* DeadpanSnarker
* ExpressiveMask
* GeniusBruiser
* HeroesWantRedheads
* HeroWithBadPublicity: Among the worst he has ever received by the end of the series.
* LightningBruiser
* UnstoppableRage: Unleashed upon Kraven after [[spoiler: the Gaines Twins create a mental illusion, causing him to think that Kraven killed Mary Jane]].
* YouFightLikeACow: It wouldn't be Spider-Man otherwise.


!!!Mary Jane Watson
Mary Jane has been the object of Peterís desires since he was in the 2nd grade. After previously being engaged to Flash Thompson, she later moved onto Harry before she fell for Spider-Man, after he saved her life. Later realizing that she loved Peter, Peter rejected her love because he didnít want to cause her any further harm due to his double life as Spider-Man.

She later began majoring in drama at Empire State University, where she remained good friends with Peter and Harry. She still has feelings for both Peter and Spider-Man, not knowing they are really the same person.

* BettyAndVeronica: Competes with Indy for Peter's affections.
* DistressedDamsel
* FieryRedhead
* GreenEyes
* HeroesWantRedheads
* StoodUp: Well, she ''thinks'' this is what Peter has done to her several times in the series, but really he is prevented from turning up by unforeseen circumstances (battling villains, interrogated by the FBI, involved in a hostage situation, etc).


!!!Harry Osborn
Never short of snappy one liners, Harry Osborn is the best friend of Peter Parker, the two of them live together off-campus and attend Empire State University together. Harry tends college for the social life, rather than the education, as he controls the majority of the stock from his late Fatherís company, Oscorp.

Despite being Peter and MJís best friends, he hates Spider-Man, blaming the wall crawler for his fatherís death, unaware that he was The Green Goblin. Still struggling to come to terms with his Dadís death, Harry is often found drinking or trying his luck with the ladies.

* BrilliantButLazy: As evidenced by the quote:
--> Kids. Try to get them to spend their time wisely, like, watching R-Rated [=DVDs=]. But noooo... they can't wait to do their web research.
* DeadpanSnarker: Possibly more so than even Peter in this series.
* StrongFamilyResemblance: Looks quite similar to his father, Norman Osborn. Commented upon by MJ.



[[folder:Supporting Characters]]
!!!Indira "Indy" Daimonji
* BettyAndVeronica: With MJ over Peter.
* CanonForeigner
* GirlishPigtails
* HotScoop: Aiming to be one of these eventually.
* {{Meganekko}}

!!!J. Jonah Jameson
* AlliterativeName
* DaEditor
* DeadpanSnarker

* AscendedExtra: Became Harry's girlfriend after being a background character throughout the series.
* [[YouGottaHaveBlueHair You Gotta Have Pink Hair]]


[[folder: Villains]]

!!!Lewis Wyler/Turbo Jet
* CanonForeigner
* {{Expy}}: Of Rocket Racer.

!!!Wilson Fisk/The Kingpin
* BadassNormal
* FatBastard
* GeniusBruiser
* LightningBruiser
* ManipulativeBastard: In his first appearance, he tricked Spider-Man into stealing a chip for him by pretending he was working for the government.
* RoleReprisal: The late Creator/MichaelClarkeDuncan reprises his role from ''Film/{{Daredevil}}'' movie as ComicBook/TheKingpin.
* ScaryBlackMan

!!!Curt Connors/The Lizard
* AdaptationalVillainy: Curt himself is nastier and more bitter than his usual portrayal, even pre-Lizard.
* BeastMan: Upon becoming The Lizard.
* FamilyUnfriendlyDeath: Got his arm cut off ''again'' by an air-conditioning exhaust fan, then fell from a helicopter. And his body is actually shown after the fall.
* HealingFactor: Similar to his mainstream counterpart.
* KilledOffForReal
* MadScientist
* RoaringRampageOfRevenge: His arm was lost when Oscorp were testing a new explosive device in the same area as the swamp he was gathering samples from, known as [[FunWithAcronyms WAFER]] (Wide Area Fragmentation Explosive Round).
* WallCrawling

* ActionGirl
* CanonForeigner
* HealingFactor: She can use her sword to heal wounds and gain youth by exposing it to moonlight.
* KilledOffForReal
* NobleDemon: Initially, she was hired by a rich collector to capture Spider-Man alive for him. After fighting Spider-Man for the first time and acknowledging him as a WorthyOpponent, however, she gives back the money to her hirer after admitting she can't assure she'll not kill him, even mentioning it would be dishonorable to catch him for such a reason.
* ReallySevenHundredYearsOld
* TheOnlyOneAllowedToDefeatYou: She cuts off one of her troops' trigger-fingers because he opened fire on Spider-Man, and ''no one'' decides how to take down an opponent but her.
* WorthyOpponent: Sees Spider-Man as such.

* BadassNormal
* BitchInSheepsClothing: Subverted. Despite her antagonism with Spider-Man, it looks like her kindness towards Harry, Peter, and M.J. were legitimate.
* CanonForeigner
* DatingCatwoman: Harry manages to fall for her civillian persona, Cheyanne, much to Peter's surprise.
* {{Expy}}: Of Black Cat.
* [[DaddyIssues Mommy Issues]]: She hints at them. When Spidey asks why she's doing this, Talon makes vague comments about how "It all starts with the mother."
* SassyBlackWoman

* ButtMonkey: In their encounters with Spider-Man, Alexei was usually the first guy wound up in webs.
* CanonForeigner
* {{Jetpack}}

* AntiVillain: Due to the fact that she received actual brain damage. She's nuts and she did try to kill M.J., but she legitimately believes that it's what Spider-Man wants because her mind is telling her that. Christina needs medical attention and a good psychiatrist.
* AxCrazy: It sort of helps that her psychosis manifested as a result of actual brain damage thanks to her malfunctioning ESP crown.
* CanonForeigner
* CloudCuckoolander
* {{Goth}}
** PerkyGoth: Somewhat.
* IJustWantToBeLoved
* MadScientist
* MotorMouth
* MulticoloredHair: Her hair is black with streaks of orange, yellow, and green.
* SanitySlippage: Despite her StalkerShrine, she didn't become dangerous until after her ESP crown electrocuted her. Instead of giving her powers, it made her hallucinate that her pictures of Spider-Man were talking to her and ordering her to do whatever it took to make sure they became a couple, including kill Mary Jane.
* StalkerWithACrush: For Spidey.
* WrenchWench: Not so much for cars, but she has jury rigged an acid gun, and an ESP crown, although the latter has some issues...
* {{Yandere}}

!!!Max Dillon/Electro
* AxCrazy
* DisproportionateRetribution: ''KILLED'' a college senior because he was constantly pranking him.
* IJustWantToHaveFriends
* KilledOffForReal
* PsychoElectro
* WoobieDestroyerOfWorlds: He has some issues, yes, but it's hard not to feel bad for the guy.

!!!Dr. Zellner
* CanonForeigner
* MadScientist.

!!!The Biker Twins/Jack and Mack
* CanonForeigner
* DumbMuscle
* ThoseTwoBadGuys

* BadassNormal
* EnemyMine: Works together with Kraven, a guy she would usually kill, to get vengeance on Spider-Man. [[spoiler:But that's just an illusion.]]
* HeroAntagonist: Though she was attempting to assassinate an important politician, she had really good reasons to do so; the man was actually a terrorist attempting to infiltrate the White House.
* KnightTemplar: Her mission had a honorable goal, but she was willing to use methods such as stealing weapons, taking teenagers hostages (and wanting to kill them herself when it didn't work as planned), and putting a bomb on the mayor's car.
* NeverFoundTheBody

!!!The Gaines Twins/Roland and Roxanne
* CanonForeigner
* CreepyTwins
* KilledOffForReal
* {{Manipulative Bastard}}s
* MindControl
* NonActionGuy: Roland. When he makes the notion that they should be trying to kill Kraven themselves, rather than mind-controlling Spider-Man into doing it, Roxanne points out that his senses are too keen in order for them to get close enough and brings up a NoodleIncident of "the last time you [Roland] used your tiny little hands to fight." Later when he switches from using his mental powers to attempting to attack Spidey with a pipe, he does indeed get his ass kicked.
* YouKilledMyFather: Kraven the Hunter captured their parents for scientific experimentation, which eventually killed them, but it also gave Roland and Roxanne their mental abilities. Rather than try and kill him themselves however, they opt for Spider-Man to do it by manipulating him.