[[folder:Main Characters]]
!!!Peter Parker/Spider-Man
Bitten by a radioactive spider on a school field trip, young Peter Parker attempted to cash in on his new powers, and signed up for a pro wrestling contest, and pinned the champion. When the promoter cheated him out of his winnings, Peter stood by as a thief robbed the promoter.

The same thief later killed his Uncle. Peter began using his newfound powers responsibly and began fighting crime. One of the first criminals he faced was The Green Goblin. When The Goblin learned that it was Peter under the mask, he attacked his Aunt May, and kidnapped the girl he loved, Mary Jane. After a dramatic battle, The Green Goblin revealed himself to be Norman Osborn. Seconds later, The Goblin was killed when he accidentally impaled himself on his glider.

He later began studying at ESU, along with his dream girl Mary Jane and his best friend Harry, the son of The Green Goblin. Harry isnít aware that his Dad was The Green Goblin, and hates Spider-Man, believing he murdered him. Peter makes his living by selling pictures of Spider-Man to J. Jonah Jameson. After being ripped off one too many times, he decided to start selling video footage to Empire 1, where he met Indy, a research assistant who took a great shining to Peter.

Peter is still in love with Mary Jane, but feels that being with her would only jeopardize her safety again, which often leads to awkward feelings between the two of them, especially as he recently began seeing Indy [[spoiler: before he was tricked into throwing her off a building by the Gaines twins]].

[[spoiler: Vowing never to let Spider-Man hurt anyone else, Peter threw his Spider-Man costume into the river and stopped being Spider-Man]].

* AdaptationalAttractiveness: Looks NOTHING like the movie version of Peter the show is based on. In fact, this Peter shares an even stronger resemblance towards his comic book counterpart than Creator/TobeyMaguire does.
* BadassBookworm
* BewareTheNiceOnes: Kraven found this out the hard way.
* ComesGreatResponsibility: Averted; This is the first adaptation do so but Peter never uses the trademark phrase nor does he ever refer to it.
* DeadpanSnarker
* ExpressiveMask
* GeniusBruiser
* HeroesWantRedheads: As per usual (and just like in the films), Peter is very in love with Mary Jane (who has short, red hair).
* HeroWithBadPublicity: Among the worst he has ever received by the end of the series.
* LightningBruiser
* UnstoppableRage: Unleashed upon Kraven after [[spoiler: the Gaines Twins create a mental illusion, causing him to think that Kraven killed Mary Jane]].
* YouFightLikeACow: It wouldn't be Spider-Man otherwise.


!!!Mary Jane Watson
Mary Jane has been the object of Peterís desires since he was in the 2nd grade. After previously being engaged to Flash Thompson, she later moved onto Harry before she fell for Spider-Man, after he saved her life. Later realizing that she loved Peter, Peter rejected her love because he didnít want to cause her any further harm due to his double life as Spider-Man.

She later began majoring in drama at Empire State University, where she remained good friends with Peter and Harry. She still has feelings for both Peter and Spider-Man, not knowing they are really the same person.
* AdaptationalDyeJob: While her hair color is still red, The length of MJ's hair length here is pretty short compared to the traditional long and red hair that the Mary Jane of the comics (both Original and Modern) and other adaptations are usually depicted with having.
* BettyAndVeronica: Competes with Indy for Peter's affections.
* DistressedDamsel: Though she ''is'' able to defend herself from time to time, and was the reason Spider-Man figured out destroying Shikata's sword would stop her. She even tried to do it herself.
* FieryRedhead: As per usual. But, as said above, The length of MJ's hair is noticeably shorter than any other versions.
* GreenEyes
* HeroesWantRedheads
* NotSoDifferent: After her ordeal with Christina, she suddenly realizes as she talks about it with Peter that Christina's whole "soul mates" obsession is too similar to her earlier attempts at forcing Peter to share intimate knowledge with one another.
* StoodUp: Well, she ''thinks'' this is what Peter has done to her several times in the series, but really he is prevented from turning up by unforeseen circumstances (battling villains, interrogated by the FBI, involved in a hostage situation, etc).


!!!Harry Osborn
Never short of snappy one liners, Harry Osborn is the best friend of Peter Parker, the two of them live together off-campus and attend Empire State University together. Harry tends college for the social life, rather than the education, as he controls the majority of the stock from his late Fatherís company, Oscorp.

Despite being Peter and MJís best friends, he hates Spider-Man, blaming the wall crawler for his fatherís death, unaware that he was The Green Goblin. Still struggling to come to terms with his Dadís death, Harry is often found drinking or trying his luck with the ladies.

* AdaptationDyeJob: Creator/JamesFranco has reddish-brown hair in the role and most other versions of Harry are straight-up redheads. This Harry is blond.
* TheAlcoholic: Was heading this way. Nearly every episode had him drinking something with alcohol in it.
* BrilliantButLazy: As evidenced by the quote:
--> Kids. Try to get them to spend their time wisely, like, watching R-Rated [=DVDs=]. But noooo... they can't wait to do their web research.
* DeadpanSnarker: Possibly more so than even Peter in this series. As opposed to his comic book incarnation.
* StrongFamilyResemblance: Looks quite similar to his father, Norman Osborn. Commented upon by MJ.



[[folder:Supporting Characters]]
!!!Indira "Indy" Daimonji
* BettyAndVeronica: With MJ over Peter.
* CanonForeigner: She was created for the series in order to be an amalgamation of Gwen Stacy and Betty Brant.
* DeadpanSnarker
* GirlishPigtails
* HotScoop: Aiming to be one of these eventually.
* {{Meganekko}}
* [[spoiler: StuffedInTheFridge: Peter is tricked into pushing Indy off the roof of a building by the Gaines twins. The series ends with Indy in a coma, and her chances of awaking were slim to none. This is one of the factors in Peter giving up being Spider-Man.]]

!!!J. Jonah Jameson
* AlliterativeName
* DaEditor
* DeadpanSnarker: As opposed to his comic counterpart or other versions (who are usually portrayed as [[LargeHam Large Hams]])
* DemotedToExtra: is nowhere near as prominent as his comic counterpart or other versions.

* AscendedExtra: Became Harry's girlfriend after being a background character throughout the series.
* CanonForeigner
* [[YouGottaHaveBlueHair You Gotta Have Pink Hair]]


[[folder: Villains]]

!!!Lewis Wyler/Turbo Jet
* BitchInSheepsClothing: When he fights Spider-Man in public, he claims he's making a difference for the people. In private, he calls the very same people stupid for assuming he's a hero in the first place.
* CanonForeigner: was created for the series.
* DirtyCoward: Seriously hurts a random bystander in his efforts to make an escape.
* {{Expy}}: Of Rocket Racer.
* InsufferableGenius: He has a very low opinion of pretty much everybody around him.
* KickTheDog: The minute his facade gets blown, he tosses a decoy he was trying to steal at an innocent bystander and severely injures her. She can even be heard sobbing in pain.
* StarterVillain: is chronologically the first villain to be fought by Spider-Man in the series.
* VillainWithGoodPublicity: He becomes beloved by the people living in Villeroy Towers because circumstances led them to think he's helping them in their protests to keep their homes. In his last fight with Spider-Man, Spidey reveals to the protesters just how little Turbo Jet actually cares about them. In his frustration at being backed in a corner, he pretty much confirms what Spidey reveals.

!!!Wilson Fisk/The Kingpin
* BadassNormal
* FatBastard
* GeniusBruiser
* LightningBruiser
* ManipulativeBastard: In his first appearance, he tricked Spider-Man into stealing a chip for him by pretending he was working for the government.
* RoleReprisal: The late Creator/MichaelClarkeDuncan reprises his role from ''Film/{{Daredevil}}'' movie as ComicBook/TheKingpin.
* ScaryBlackMan

!!!Curt Connors/The Lizard
* AdaptationOriginConnection: In this version, he lost his arm to what's implied to be the prototype of what became the Green Goblin's pumpkin bombs.
* AdaptationalVillainy: Curt himself is nastier and more bitter than his usual portrayal, even pre-Lizard.
* BeastMan: Upon becoming The Lizard.
* DeathByAdaptation: He's alive and well in the comics.
* FamilyUnfriendlyDeath: Got his arm cut off ''again'' by an air-conditioning exhaust fan, then fell from a helicopter. And his body is actually shown after the fall.
* HealingFactor: Similar to his mainstream counterpart.
* KilledOffForReal
* MadScientist
* PetTheDog: Before Curt became the Lizard, he was saved by Spider-Man during a robbery. As he's giving a statement to the police, he gets slightly pissed at the officer claiming Spider-Man and the robbers were working together even though he just said Spider-Man ''saved'' him.
* RoaringRampageOfRevenge: His arm was lost when Oscorp were testing a new explosive device in the same area as the swamp he was gathering samples from, known as [[FunWithAcronyms WAFER]] (Wide Area Fragmentation Explosive Round).
* WallCrawling

* ActionGirl
* CanonForeigner
* {{Expy}}: Despite being created for the series, She received the role of traveling from a foreign country into New York City in order to hunt and kill Spider-Man that is usually associated with Kraven the Hunter (who does show up later in the series).
* FaceDeathWithDignity: Starts looking very despondent when her sword is broken, but she recomposes herself and bows to Spider-Man, acknowledging his victory.
* HealingFactor: She can use her sword to heal wounds and gain youth by exposing it to moonlight.
* {{Hypocrite}}: She talks about honor, but is more than willing to use M.J. as leverage to finally get her fight with Spider-Man. Also, despite earlier making comments about not being scared by death, she visibly loses her cool the moment Spider-Man gets her sword (the thing that keeps her young). At the very least, when the sword gets broken, she bows in defeat before dying.
* KilledOffForReal
* NobleDemon: Initially, she was hired by a rich collector to capture Spider-Man alive for him. After fighting Spider-Man for the first time and acknowledging him as a WorthyOpponent, however, she gives back the money to her employer after admitting she can't assure she'll not kill him, even mentioning it would be dishonorable to catch him for such a reason.
* NoImmortalInertia: She dies seconds after her sword is broken.
* ReallySevenHundredYearsOld
* OffWithHisHead: She kills the guy who hired her to capture Spider-Man after she calls of their deal because he was interrupting things between her and Spidey.
* TheOnlyOneAllowedToDefeatYou: She cuts off one of her troops' trigger-fingers because he opened fire on Spider-Man, and ''no one'' decides how to take down an opponent but her.
* WorthyOpponent: Sees Spider-Man as such.

* BadassNormal: She's just a regular thief with no super powers, relying entirely on her gadgets to fight and perform heists. Despite this she's still able to match Spider-Man in fights and evade him.
* BitchInSheepsClothing: Subverted. Despite her antagonism with Spider-Man, her kindness towards Harry, Peter, and M.J. was genuine.
* CanonForeigner: She has no comic book counterpart, but is based on Black Cat.
* DatingCatwoman: Harry manages to fall for her civilian persona, Cheyanne, much to Peter's surprise. This is the reason why Spider-Man tries to get her to stop being a criminal.
* DontYouDarePityMe: She gets annoyed at Spider-Man's frequent attempts to figure out her FreudianExcuse and his constant pleading that she's better than this, demanding to know why he even gives a crap.
* {{Expy}}: Of Black Cat.
* ItsPersonal: She's a thief who is only interested in stealing objects that have emotional value to the people they belong to, saying if it's important to them that makes them worth stealing.
* [[DaddyIssues Mommy Issues]]: She hints at them. When Spidey asks why she's doing this, Talon makes vague comments about how "It all starts with the mother."
* SassyBlackWoman
* SugarAndIcePersonality: A SpoiledSweet rich girl who Harry has serious feelings for and saves M.J. at one point, and also a cat burglar only interested in stealing things people would genuinely be despondent to lose and willing to manipulate the police into getting Spider-Man off her back.
* TakeThatKiss: Not a physical example, but she blows to kisses to Spider-Man to taunt him during their first fight. First, after she kicks him away and puts her goggles back on she turns back to him and blows a kiss before running off. Then, after Spider-Man is stuck holding a piece from a construction building in place to stop it from falling into the streets, Talon blows him another goodbye kiss to taunt him before dissappering.

* ButtMonkey: In their encounters with Spider-Man, Alexei was usually the first guy wound up in webs.
* CanonForeigner
* {{Jetpack}}

* AntiVillain: Due to the fact that she received actual brain damage. She's nuts and she did try to kill M.J., but she legitimately believes that it's what Spider-Man wants because her mind is telling her that. Christina needs medical attention and a good psychiatrist.
* AxCrazy: It sort of helps that her psychosis manifested as a result of actual brain damage thanks to her malfunctioning ESP crown. That being said, she takes M.J. hostage with her acid gun and it makes it clear she'll use it on her, laughing at the idea of her organs splayed out on the ground in a puddle.
* CanonForeigner
* CloudCuckoolander
* EyeTwitch: During her last attempt on M.J.'s life.
* {{Goth}}
* IJustWantToBeLoved
* KilledOffForReal: Averted. Despite being trapped in an elevator as it plummeted to the ground, she just ended up on a stretcher with a neck brace.
* LoonyFan: Oh so very much. She believed she was soul-mates with Spider-Man long before she got zapped.
* LoveMakesYouEvil: As long as she can get Spider-Man, she doesn't seem to think there's anything wrong in committing murder to get him despite one of the things she finds attractive about him is his heroism.
* LovingAShadow: She loves Spider-Man, but more precisely she loves the image of him and the hallucinations her brain is creating. Christina thinks the only reason the real Spider-Man isn't with her now is because he doesn't know he loves her and needs coaxing.
* MadScientist: She built a glue gun in high school that turned out to be highly acidic and dangerous, and her ESP crown actually does let her read minds. The wiring is a mess, though.
* MotorMouth
* MulticoloredHair: Her hair is black with streaks of orange, yellow, and green.
* {{Nosebleed}}: Whenever she hallucinates or whenever she is acting particularly psychotic her nose will begin to bleed, most likely an indicator of her brain damage.
* PerkyGoth: Very excitable and claims she can get along with almost anyone.
* PsychopathicManchild: As she has M.J. hostage:
-->'''Mary Jane''' (''Scared''): How do you know it's you Spider-Man wants? How do you know it's not someone else?
-->'''Christina''' (''Laughing and holding her acid gun to M.J.'s face''): Then ya better hope it's not ''you!''
* RescueRomance: Tries to pull this off. She expects Spider-Man will save her when she walks off the roof of a ''very'' tall building, and he does. But the "romance" part doesn't take off. She gets sad and angry later on because she was "Just trying to be a good girlfriend."
* SanitySlippage: Despite her StalkerShrine, she didn't become dangerous until after her ESP crown electrocuted her. Instead of giving her powers, it made her hallucinate that her pictures of Spider-Man were talking to her and ordering her to do whatever it took to make sure they became a couple, including kill Mary Jane.
* SlasherSmile: Especially after she goes after M.J..
* StalkerShrine: Naturally she has one to Spider-Man in her closet.
* StalkerWithACrush: For Spidey.
* ThatLiarLies: When M.J. asserts she's not interested in Spider-Man. "You LIE! I don't like liars!"
* WrenchWench: Not so much for cars, but she has jury rigged an acid gun, and an ESP crown, although the latter has some issues...
* {{Yandere}}

!!!Max Dillon/Electro
* AxCrazy
* BecauseYouWereNiceToMe: Why he tries to convert Sally into an electrical being like him, thinking she actually loves him.
* CompanionCube: Ditches Peter for a partner in a class project, for a AI computer version of himself.
* DisproportionateRetribution: ''KILLED'' a college senior because he was constantly pranking him. To be fair, one of those pranks nearly got him DROWNED IN CEMENT!
* FaceHeelTurn: He started off as one of Peter's friends from high school and didn't turn into a villain until halfway into the show.
* IJustWantToHaveFriends
* KilledOffForReal
* OneWingedAngel: When drained of electricity, he turns into a thin, clawed version of himself
* PsychoElectro
* ShockandAwe: How he received his powers, just like in the comics.
* WoobieDestroyerOfWorlds: He has some issues, yes, but it's hard not to feel bad for the guy.

!!!Dr. Zellner
* CanonForeigner
* {{Expy}}: He reminds many fans of Miles Warren, the mastermind of the ''[[ComicBook/TheCloneSaga The Clone Saga]]''.
* MadScientist.

!!!The Biker Twins/Jack and Mack
* CanonForeigner
* DumbMuscle
* ThoseTwoBadGuys

* BadassNormal
* EnemyMine: Works together with Kraven, a guy she would usually kill, to get vengeance on Spider-Man. [[spoiler:But that's just an illusion.]]
* HeroAntagonist: Though she was attempting to assassinate an important politician, she had really good reasons to do so; the man was actually a terrorist attempting to infiltrate the White House.
* KnightTemplar: Her mission had a honorable goal, but she was willing to use methods such as stealing weapons, taking teenagers hostages (and wanting to kill them herself when it didn't work as planned), and putting a bomb on the mayor's car.
* NeverFoundTheBody

!!!Kraven The Hunter/Sergei Kravenoff
* BadassBaritone: Due to having Creator/MichaelDorn as his voice actor.
* BadassMustache
* BadassNormal: As per usual, Kraven is a skilled hunter whom Spider-Man defeated prior to the series and he does not appear to have any superhuman abilities.
* BoisterousBruiser/BloodKnight: Zigzagged with both.
* DecompositeCharacter: The role of traveling from a foreign country into New York City in order to hunt and kill Spider-Man (due to being contacted by a criminal mastermind that hates the Web-Slinger and wants him dead) that is usually associated with the Kraven of the comics and most versions is given to CanonForeigner Shikata.
** Although, the Kraven of this series still portrayed a skilled hunter from Russia whom Spider-Man has encountered prior to the series.
* EgomaniacHunter: He was willing and able to hunt and kill anything (particularly the parents of the Gaines Twins) until he fought Spider-Man.
* EnemyMine: He works together with Silver Sable in order to get vengeance on Spider-Man. [[spoiler:But it's just an illusion.]]
* HuskyRusskie: His Russian accent is not as emphasized as the other versions, but it's still there.
* KilledOffForReal: Subverted; He was about to be this during a battle that he fought with Spider-Man in an empty power plant when the Web Slinger was about kill him (through nearly hanging Kraven! Now that's harsh!) after [[spoiler: the Gaines Twins created a mental illusion that caused Spider-Man to believe that Kraven killed Mary Jane]]. But fortunately for Kraven, Spidey snaps out of it (after he saw outside the ESU science sign when he remembered it was ripped off), relents his decision, and turns Kraven over to the police.
* MovieSuperheroesWearBlack: An animation (and super villain) example. This Kraven wears a black leather jacket instead of the lions mane vest of the comics and most adaptations.

!!!The Gaines Twins/Roland and Roxanne
* CanonForeigner
* CreepyTwins
* KilledOffForReal
* {{Manipulative Bastard}}s
* MindControl
* NonActionGuy: Roland. When he makes the notion that they should be trying to kill Kraven themselves, rather than mind-controlling Spider-Man into doing it, Roxanne points out that his senses are too keen in order for them to get close enough and brings up a NoodleIncident of "the last time you [Roland] used your tiny little hands to fight." Later when he switches from using his mental powers to attempting to attack Spidey with a pipe, he does indeed get his ass kicked.
* YouKilledMyFather: Kraven the Hunter captured their parents for scientific experimentation, which eventually killed them, but it also gave Roland and Roxanne their mental abilities. Rather than try and kill him themselves however, they opt for Spider-Man to do it by manipulating him.