A list of characters found in Creator/{{Disney}}'s ''ComicBook/MickeyMouseComicUniverse''.

'''Keep in mind that since the characters and series [[PrintLongRunners have been around for so long]], whether a character displays certain traits or not in any given story largely depends on the [[DependingOnTheArtist artist]], [[DependingOnTheWriter the writer]], [[EraSpecificPersonality or the time period]].'''
* [[Characters/MickeyMouseComicUniverseProtagonists The Protagonists]] [[labelnote:Click to expand.]]
** '''Main heroes:''' Mickey Mouse, Minnie Mouse, Goofy, Clarabelle Cow, Horace Horsecollar, Pluto
** '''Neighbors:''' Butch, Ellsworth Bheezer, Glory-Bee, Patricia Pigg, Percy Pigg, Annabelle Mink, Gideon and Gertie Goat, Sieb Sik
** '''Friends:''' Felicity Fieldmouse, Madeline Mouse, Arizona Goof, Martina Ubersetzen, Eurasia Toft, Katarina Kodorofsky, Captain Doberman, Gloomy, Cosmo "Doc" Static, Doctor Einmug, Professor Deeping Dustibones, Professor Wagstaff, Doctor Oofgay, Estrella Marina, Eega Beeva, Atomo Bleep-Bleep, Charlie, Weegie, Mimi, Professor Cicero Webster, Captain Nathaniel Churchmouse, Sam Simian, King Michael XIV and Queen Phylline, Yoyo
** '''Children:''' Bertie, Ellroy, Gilbert, Itsy-Betsy, Millie and Melody, Mortimer and Ferdinand Fieldmouse
** '''Mouseton Police Department:''' Chief Samus O'Hara, Detective Casey, Rock Sassi, Officer Clancy, Shamrock Bones
** '''Acquaintances:''' Mortimer Mouse, Montmorency Rodent, Zenobia II, Former sergeant, Joe Piper and co., Muscles [=McGurk=], The Princess, Dan
** '''Animals:''' Tanglefoot[[/labelnote]]
* [[Characters/MickeyMouseComicUniverseAntagonists The Antagonists]] [[labelnote:Click to expand.]]
** '''Main villains:''' Pete, The Phantom Blot, Emil Eagle
** '''Allies of Pete:''' Trudy Van Tubb, Chirpy Bird, Sylvester Shyster, Eli Squinch, Von Weasel, Monkey scientist, Portis, Dexter Dingus, Muck Raker, Scuttle/Weasel, Dum-Dum, Doc Wombat
** '''Allies of the Phantom Blot:''' Mr. X, Alberta, The Phantom Brat
** '''Supernatural villains:''' Doctor Doom, Drusilla, Mr. Fox, Doctor Grut, Phantasmo, The Imp, The Phantom Gondolier, Princess Queezila
** '''Half-villains:''' Delilah, Hickup, Kat Nipp, Meanie Markham, Swifty[[/labelnote]]
* [[Characters/MickeyMouseComicUniverseSubseries Characters from various subseries]] [[labelnote:Click to expand.]]
** '''''Mickey and the Sleuth'':''' Sureluck Sleuth, Professor Nefarious, Fliplip, Armadillo and Sidney
** '''''Super Goof'':''' Doctor Darius Dunk, Doctor Syclocks, Flame Knight, Mr. Mystery-Man, Super Thief, Doctor Tempo, Tuffy Taurus
** '''''X-Mickey'':''' Manny, Pipwolf[[/labelnote]]
* [[Characters/DisneyDucksComicUniverse Characters that belong to the Disney Ducks Comic Universe]]
* [[Characters/DisneyMouseAndDuckComics Characters that belong to the Mouse & Duck Comic Universe]]