In order of (more or less) their first appearance in the anime... (Will probably use a different sorting method in time.)

!!! Several characters share the following tropes:

* AnthropomorphicPersonification
* MeaningfulName
* NightmareFuelStationAttendant
* OneSceneWonder

!!! Gregory

* AnthropomorphicPersonification: It is ''very'' strongly suggested he's the physical form of (lost) desires and dreams...his hotel/world is a manifestation of chaos.
* ButtMonkey: Becomes this in ''The Bloody Karte''
* CrustyCaretaker
* DirtyOldMan: He has a collection of porn magazines, makes a few suggestive comments to Second Guest, and in ''Bloody Karte,'' he gropes Cactus Girl.
* EvilLaugh: Well, more of a chuckle, but still...
* FauxAffablyEvil: Oh sure, he'll say he wants to help the guests, offer them a place to stay, and calls the Guests "my friend/my dear," but he'll do it while breaking their psyches, swallowing human souls, and actively planning to feed them to his mother.
* OneWingedAngel
* PetTheDog: He has his moments--mainly in the third and fourth seasons.
* VillainProtagonist: The third season focuses on him instead of a guest.
* YouDirtyRat

!!! The Guest
--> The Guest is the main character of the first two seasons, with everything seen from their eyes. The First Guest is a working man with a wife and son, and the Second Guest is a working woman who struggles with the decision of CareerVersusMan.

* [[spoiler:AndThenJohnWasAZombie]]
* BadassNormal: The Second Guest deserves this for the fact that at one point she, rather than just take Gregory's BS, ''picks up an ax and chases him off with it.''
* [[spoiler: [[DownerEnding Downer]] / BittersweetEnding: Neither Guest manages to escape the hotel, and returns to stay forever. The First Guest quite likely becoming Haniwa Salaryman, and the Second Guest becomes a member of Gregory's family.]]
* TheFaceless
* NoNameGiven
* PetTheDog: Both guests engage in this more than once.
* POVCam

!!! Neko Zombie

* CatsAreMean: Actually he's not a bad guy and genuinely wants to help guests escape if he can, but he's understandably bitter about what was done to him and so he can be snappish. Approaching him during certain times in the game, specifically when he's very hungry, will cause him to attack you.
* DontYouDarePityMe
* [[FearOfThunder Fear Of Thunder/Lightning]]: This is because it was during a thunder/lightning storm that ''someone'' stitched him up.
* HiddenDepths
* KillItWithFire: How he destroys the hotel and tries to kill Gregory. [[spoiler:It doesn't work.]]

!!! Lost Doll

* CreepyChild
* CreepyDoll
* SuperpoweredEvilSide: Implied in ''The Last Train''
* TheWoobie: When she's not screaming about her dollie, anyway.

!!! Hell's Chef
* BerserkButton: '''NO SMOKING!'''
* {{BFS}}
* EnsembleDarkhorse
* {{Pride}}
* WeaksauceWeakness: Gusts of air will blow out the flame on his head, causing him to stop moving.

!!! Catherine

* DeadlyDoctor
* GirlWithPsychoWeapon
* IfICantHaveYou: Has shades of this in ''The Bloody Karte,'' such as when [[spoiler:she kills Haniwa Salaryman after he announces his wife wants to get back together with him.]]
* {{Yandere}}

!!! Mummy Dog

* ChildrenAreInnocent: One of the only residents who isn't consciously malicious.
* FailedASpotCheck: Has an ax in his head, which he doesn't seem to acknowledge. He knows he has head-aches, but thinks he just inherited them from his dad.

!!! Mummy Papa

* FailedASpotCheck: Has an Arabian sword in his his son, he doesn't know it's there. Despite constant splitting headaches.

!!! Judgment Boy

* CatchPhrase: "Do you know / Who I am? / They call me / Judgment Boy!"
** "JUDGMENT now!"
* EnsembleDarkhorse
* InsaneTrollLogic: Perhaps not outright ''insane'' logic, but the scenarios he presents and the consequences of the choices made don't necessarily have to happen the way he tells them. Then again, in most cases he's reminding the guests of things that already happened.
* LargeHam
* SadisticChoice: His purpose to to present these to the Guest. ''Hopefully'' they're just hypothetical.

!!! Cactus Gunman

* AbhorrentAdmirer: for the Second Guest.
* ATeamFiring: He ''very'' seldom hits what he aims at.
** And he ''almost always'' hits Gregory.
* TheGunslinger
* {{Yandere}}: for the Second Guest.

!!! TV Fish

* TheVoiceless

!!!Public Phone

* ConArtist

!!! Prompters

* AllThereInTheManual: Twin stage hands who aren't talking to each other.
* TheFaceless
* TheVoiceless

!!! Gregory Mama

* AbusiveParents
* EvilMatriarch
* VainSorceress: Subverted in that she's not beautiful at all, she just ''considers'' herself beautiful.
* WickedWitch
* YouDirtyRat

!!! Clock Master & My Son

* CatchPhrase: "TIME IS MONEY!!"
* TimeMaster

!!! Frog Fortune-Teller

* FortuneTeller
* SavePoint: in the PS2 game.
* YouCantFightFate: "Never try to argue with fate..."

!!! Toilet Baby

!!! Roulette Boy
* CatchPhrase: "SPIN ME!"
** MostAnnoyingSound

!!! Speed Mouse & Mutant Ducks

* NoOSHACompliance

!!! Mirror Man

* MagicMirror
* TheMirrorShowsYourTrueSelf: He claims he does, but it's hard to say if it's true.

!!! Kinko and Inko

* {{Engrish}}: "Secret N'''o'''mber."
* HeterosexualLifePartners

!!! Angel/Devil Dog

* SplitPersonality

!!! Dead Body

* TrademarkFavoriteFood: Alcohol
* WeaksauceWeakness: Water and wind.

!!! Judgment Boy Gold

* LargeAndInCharge: The leader of the Judgment Boys, he's ''much'' bigger than they are.
* [[LargeHam LargER Ham]]

!!! Death

* DontFearTheReaper: One of the only two characters (besides Neko Zombie in the PS2 game) who's genuinely kind to the Guest.
* TheGrimReaper
* TheOtherDarrin: In the English dub he was apparently played by one man, but had three different voices and personalities: one at the end of the first season who was very no-nonsense (understandable, given the situation) and outright told the guest that he might not be happy going home, one for the rest of the series who was calmer and gentler, and one in the game who was much more upbeat and had a very noticeable Brooklyn accent.

!!! Stephen Haniwa Salaryman

* [[spoiler:AndThenJohnWasAZombie]]: Canon ''very'' heavily suggests [[spoiler:that he is what the First Guest has become.]]
* EmptyShell: What the monotony of his job has turned him into.
* {{Salaryman}}: [[MeaningfulName It's in his name]] [[ExactlyWhatItSaysOnTheTin for a reason, you know]].

!!! James

* BrattyHalfPint
* CreepyChild: A bit.
* HotelHellion
* KarmaHoudini
* YouDirtyRat

!!! Sleepy Sheep

* AnxietyDreams: the kicker? They are YOUR dreams...
* CountingSheep: He tries.
* TalkingInYourSleep

!!! Bonsai Kabuki
* CovertPervert
* ThirdEye: Looking into it reveals your fantasies to both you... AND him.

!!! Cactus Girl

* ActionGirl
* MurderTheHypotenuse: In ''The Second Guest,'' she 's furious at the female guest for rejecting her brother, and believes Gregory to be her she threatens to kill him if the guest doesn't date her brother.

!!! Trap Mouse

!!! Hell's Taxi

* TheTaxi

!!! Poor Conductor

* DreadfulMusician

!!! Umbaba

* EarWorm: InUniverse. His song is so catchy, anyone that hears it starts dancing along... which eventually jars the dancer's soul right of their body.

!!! Mono Eye Wizard

!!! Street Vendor

!!! Pig Gentleman

* BeCarefulWhatYouWishFor: "I want you to serve me the most tender meat you have available!"
* JerkAss: He gets what's coming to him, though...
* LetsMeetTheMeat

!!! Mummy Mama

* FailedASpotCheck: she has a ''plant'' that grows from ''her head''. Though the website implies that the reason she doesn't notice this is that the plant is draining her intelligence.

!!! Musha Dokuro

* HeelFaceRevolvingDoor: His loyalty can change around in a heartbeat.

!!! Fat Chicken

* BigEater: No one could possibly eat that many boxed lunches- oh no wait.
* LetsMeetTheMeat

!!! Bone Head

* DemBones

!!! Egypetit

!!! Dr. Fritz

* ButtMonkey: ''Hooo'' boy.
--> (after Catherine [[BulletProofHumanShield uses him to block a bullet]]) "Why? Why always me?"