This character sheet includes the major characters in the ''VideoGame/EarthwormJim'' franchise. Because [[WesternAnimation/EarthwormJim the animated series]] portrayed some characters in a very different fashion to [[VideoGame/EarthwormJim the games]], appropriate character sheets will divide their profiles between a Games version and a Cartoon version.



[[folder:Earthworm Jim]]
'''Game:''' Once an ordinary earthworm from somewhere in Texas, Jim was mutated into a new form of life when an ultra high tech indestructible super space cyber suit (more commonly called just "the Super Suit") fell from space and landed atop him. Narrowly evading the bounty hunter Psycrow, Jim learned of the existence of the evil Queen Slug-for-a-Butt and her beautiful sister Princess Whats-Her-Name, and set out to see the princess.

'''Cartoon:''' As in the game, Jim was created when Psycrow lost the Super Suit and it landed on a Texan earthworm. Unlike the game, Jim instead set out to become a superhero, for reasons unknown. Flamboyant, naive, simple-minded and at least a little crazy, Jim is nevertheless one of the best heroes in the known universe. Voiced by Creator/DanCastellaneta.
* AllMenArePerverts: In the cartoon.
--> (Finding a huge cake) "Hey! Nice cake! Is a girl gonna jump out?"
* UsefulNotes/AmericanAccents: In the game, Jim has a slight but undeniable Texan Drawl accent. The cartoon drops this in favor of a hammy superhero-esque dialect.
* {{BFG}}: Subverted. Jim's primary gun is a small handheld ray-gun. It still packs a massive amount of firepower, and it's a rapid-fire weapon.
** In the second game, Jim gets the Barn Burner, a SmartBomb weapon so big that he literally struggles to lift it.
* BreakoutCharacter[=/=]GuestFighter: Jim is the only character that appears in other games than this series, mostly in {{Fighting Game}}s:
** First as a SecretCharacter for PC version of ''VideoGame/BattleArenaToshinden'', which also was distributed by [[Creator/PlaymatesToys Playmates Interactive]], in which he was just a MovesetClone of Rungo.
** But his most famous appearance outside his game series is in ''[[VideoGame/ClayFighter ClayFighter 63 1/3]]'' and its {{sequel}} ''Sculptor's Cut'' (again as SecretCharacter) for UsefulNotes/{{Nintendo64}}. In both games, Jim is on vacation after his adventures and came to the Klaymodo Island, where he joined heroes to stop [[MadScientist Dr. Kiln]] of convert the world into clay. And also has VideoGame/{{Boogerman}} as TheRival on these games. In both, his voice actor is Creator/DanCastellaneta as in the TV series.
* BroughtDownToNormal: Relatively speaking, to the extent that a hyper-evolved worm in a super-suit can be: Jim once had his super-suit replaced with one that one that gave him the strength of a normal person. A normal, really big person, as Professor Monkey-for-a-Head found out the hard way.
* {{Catchphrase}}: "Groovy!" (extended to "Guh-roovy!" in the cartoon)
** [[MadLibsCatchphrase "EAT DIRT, [disparaging description of enemy]! AH-HAHAHAHAHAHAHAHAHA!"]]
** "By the Great Worm Spirit" ([[MadLibsCatchphrase et cetera...]])
** In Season 2: "Don't question me..."
*** Also: "Get off my back, man!"
** And don't forget the ones from ''VideoGame/ClayFighter 63 1/3'':
*** "I'm a superhero!"
*** "Eat Cow!"
*** "Tumbleworm!"
*** "Earthwormmm Jimmmm!", said it as [[Wrestling/{{WWE}} Mr. Kennedy]].
* ClothesMakeTheSuperman: Jim is only sentient because of the energy from his suit mutating him from being an ordinary annelid. Although the boost to size and intellect is permanent, all of his strength, firepower and physical abilities are due to his suit.
* HeroicComedicSociopath: Jim's plan B boils down to "blast away randomly at any viable target while laughing maniacally".
-->'''Jim:''' Oooo-kay. When all else fails... [[TriggerHappy START BLASTING!!!]] AH-HAHAHAHAHAHA!!! ''(shooting at a black hole)'' EAT DIRT, INSUFFERABLE SPACE ANOMALY!!!
* IdiotHero: Jim in the cartoon zigzags between this, occasionally coming up with some clever ideas to defeat his enemies, but generally being rather dense.
* LargeHam: In the cartoon.
* MismatchedEyes: His left eye is green, while the right one appears irisless (they both have pupils, though).
* PoweredArmor: The Super Suit, natch. In the games, it runs on atomic power; in the cartoon, it runs off a "Battery of the Gods". Unusually for the trope, it doesn't posess built-in flight systems- rather, Jim utilizes his Pocket Rocket.
* PsychopathicManchild: In the cartoons, Jim's interests include watching a kid's show ("The Mr. Bunnybutt Show"), reading a funny animals popup book, firing his gun at anything that catches his attention, and pummeling people senseless.
* ReligiousBruiser: Often prays to his god, the Great Worm Spirit and eventually gets to meet him.
* SmallNameBigEgo: The cartoon zigzags on this. Jim is crazy, egotistical and full of himself, but, more often than not, when the chips are down, he proves himself to be genuinely competent.
* WhipItGood: In the games, Jim uses himself as a whip to hit enemies as well as jump rope being grabbed by his own suit.

[[folder:Peter Puppy]]
'''Game:''' After escaping from yet another ambush by Psycrow, Jim accidentally stumbles across a wormhole and is pulled through it, crashlanding on a strange and dangerous alien world. There, he comes across an innocuous yet friendly individual, who identifies himself as Peter Puppy and explains he has the parts Jim needs to repair his Pocket Rocket and escape this hellish place; if Jim will just escort him back home... But this is easier said than done, for Peter revealed he would transform when endangered into a mindless monster that would attack Jim. Finally reaching Peter's house, Peter was good as his word, but asked to be taken away from his nightmarish homeworld as a favor, which Jim agreed to.

'''Cartoon:''' Once an ordinary dog mocked by his fellow canines for being small and cowardly, Peter dared to break the Great Taboo of the Dogs -- approaching a running vacuum cleaner -- in an effort to prove himself. But vacuum cleaners are canine-specific trans-dimensional portals, and Peter was sucked through a wormhole to the PlanetHeck, where Evil the Cat set a demonic spirit on Peter that transformed him into an anthromorphic dog -- who happened to turn into a monster when hurt, scared or enraged. Banished back to Earth, Peter was outcast as a freak, until he stumbled into the path of Earthworm Jim. After a rather hostile first meeting, Jim warmed up to his "fellow freak of nature" and eagerly took Peter on as his sidekick. Voiced by Creator/JeffBennett.
* AdaptationalDyeJob: Changed from light purple to cream coloured for the cartoon series.
* {{Adorkable}}: So. Very. Much.
* AscendedExtra: From the subject of an EscortMission in the first game to Jim's primary sidekick and righthand man.
* BadassAdorable: A terrifying monster when he's angry, an {{Adorkable}} cartoon animal the rest of the day. Yep, seems to fit the bill all right.
* BarefootCartoonAnimal: In the cartoon, at least, where he sports a shirt and shorts, but no shoes. In the games, he's completely naked.
* BerserkButton: Fear, pain, anger and Jim's constant stupidity. The first three invariably result in a HulkOut.
** {{Inverted}} slightly as Peter himself expresses in the first episode he has no control over his BerserkButton induced alter ego and spends most of it trying to apologize vigorously for brutally attacking Jim earlier on.
* BewareTheNiceOnes: BIG TIME.
* BigBallOfViolence: Whenever Jim fights Peter's Monster form.
* BlessedWithSuck: The spirit possessing him grants him intelligence and anthromophism, at the cost of going OneWingedAngel if anything pisses off, hurts, or scares him. One episode has him cured of this, but in a SugarWiki/{{Heartwarming Moment|s}}, he gains it back in order to save Jim's life.
* BroughtDownToNormal: In "The Origin Of Peter Puppy" when Jim tries to cure Peter's transformations by disposing of the demonic spirit possessing him. The spirit was unfortunately also the source of his intelligence and anthropomorphism, and its exorcism turned him back into a normal non-anthro dog.
* ButtMonkey: In the cartoon. He suffers a bit in the games too, though dishes out the abuse more often.
* {{Catchphrase}}: His MadnessMantra for when things get ''really'' bad: "[[Franchise/{{Dune}} I must not fear, fear is the mind killer, fear is the little death that brings total oblivion.]]"
* ExplosiveBreeder: By the time of ''Earthworm Jim 2'', Peter is the father of '''600 children'''.
* HalfDressedCartoonAnimal: Cartoon only (he is completely naked in the games), this is yet another result of being possessed by the demonic spirit. Peter considers it one of the better perks.
* HulkingOut: Peter is a good guy, but if he gets scared or hurt, his monstrous alter ego takes it out on whoever is in the vicinity, usually Jim.
* InvincibleVillain: In the games, you cannot avoid or fight back against Peter if he hulks out, just prevent it or expect a maiming. The cartoons justify this as he is Jim's best friend (in their first meeting, Jim mistook him for a villain and actually defeated him easily, making clear he could fight him off if he really wanted to).
* KidSidekick: In the cartoon.
* KillerRabbit: Peter looks harmless and fluffy... right up until he gets scared or injured and turns into a monster.
* TheMillstone: His HulkingOut form will nearly always attack Jim ''and only Jim''. In the first episode of the cartoon, Jim actually loses patience with Peter after another transformation earns Jim a mauling, while the bad guy gets away scot free.
* MisplacedRetribution: Whoever or whatever sets off his demonic transformation, Jim is nearly always its first target (though sometimes, Jim will do something to cause it, mostly by accident or his own stupidity). Justified in the cartoons, as Peter himself explains he has no control over himself when it happens, and is usually rather embarrassed or ashamed of it. Although, when he turns into a monster in "Bring Me the Head of Earthworm Jim", he looks genuinely confused over whether to attack Jim or Psy-Crow (who is wearing the Space Suit while Jim was left with a powerless copy). It might be the suit rather than Jim himself that draws him to attack, as he ultimately decides to attack Psy-Crow.
* [[OnlySaneMan Only Sane Pup]]: Shares this role with Princess What's-Her-Name in the cartoon.
* ServileSnarker: In the cartoon, he can switch between being a naive follower of Jim or a neurotic commentator to his bumbling from scene to scene.
* SuperpoweredEvilSide: Peter's transformation is extremely strong and dangerous... and also a mindless berserker that attacks anything nearby, usually Jim. It's even caused by DemonicPossession in the cartoon.
* UndyingLoyalty: No matter how intelligent he is, Peter is still, you know, a dog and thus he'll never leave Jim's side no matter how much he might irritate him.
* VitriolicBestBuds: With Jim in the cartoon, between Jim's antics and Peter's uncontrollable transformations, there is the odd tension to say the least, but Peter certainly hates being taken from Jim's side.
-->'''Peter:''' ''*banging his head on the table, sobbing*'' I WANNA BE JIM'S SIDEKICK AGAIN!!! I WANNA BE JIM'S SIDEKICK AGAIN!!!
* WhyDidItHaveToBeSnakes: Ever since he learned the ingredients of haggis, it's been his worst personal nightmare.
** Also vacuum cleaners, according to "The Wizard of Ooze".

[[folder:Princess Whats-Her-Name]]
'''Game:''' Sweet-hearted yet ditzy sister to Queen Slug-for-a-Butt, hearing news about the Princess is what inspires Jim to travel across the universe to save her. Their first meeting, though, is less than impressive, as a cow falls on her and knocks her out cold. In the sequel, she is kidnapped by Psycrow so he can marry her and become Ruler of the Universe.

'''Cartoon:''' Born hideously deformed by the standards of Insecticans, the Princess suffered countless humiliations at the hands of her malevolent sister, who refused to even allow her to have a name and banished her to the dungeons. Vowing to defeat and overthrow her sibling, the Princess trained throughout her childhood, growing up into a mighty warrior. While Earthworm Jim's insistence that they are a couple irritates her, she does consider him a good friend and appreciates his help in her efforts to try and defeat the Queen. Voiced by Creator/KathSoucie.
* ActionGirl: In the cartoon, she's the leader of a ragtag band of rebels and has basically been trying to overthrow her sister since she was a little girl.
* BeautyEqualsGoodness: Played straight and inverted in parody. By human standards she's supermodel level hot. By the standards of her own species she's hideously deformed. The narrator even lampshades this.
* BrainlessBeauty: In the games, according to her character profile in the manual.
** Amusingly her evil clone created by Evil Jim has this personality in the cartoon.
* CuteMonsterGirl: While an Insectican like Queen Slug-for-a-Butt, she's basically a beautiful woman with long red hair, cute little wings, and a pair of antennae. In a subversion, her own species considers her hideously malformed.
** Whereas the Queen is modeled after termite queens, the Princess (at least coloration-wise) appears to be modeled on wasps, and indeed has a [[ wasp waist]].
* DamselInDistress: In the games. Subverted in the cartoon.
* GreenSkinnedSpaceBabe: The insect wings and slightly too big eyes are the only indicators that the Princess is an alien and not a human woman.
* HumansAreUgly: An odd non-human example. The Princess despite being insectian looks like an attractive human woman, yet is considered as ugly to her own people as her sister is seen as to humans.
* ImpossibleHourglassFigure: The black-and-yellow stripes on her leggings further prove that she has a [[{{Pun}} wasp waist]].
* NonMammalianHair / RapunzelHair: She sports a red ponytail that reaches her ankles, and she's an Insectian.
* NonMammalMammaries: Once again. She's an [[HumanAliens alien bug woman]]. She has no reason to have these.
* ObliviousToLove: The Princess has no clue about Jim's romantic obsession with her, insists that his elaborate romantic evenings are NotADate, and practically makes a {{Catchphrase}} out of her denial.
---> '''The Princess:''' What do you mean, 'relationship'?''
* [[OnlySaneMan Only Sane Woman]]: In the cartoons, she shares this role with Peter Puppy.
* SuperStrength: In the cartoons, she boasts that, as an Insectican, she has the strength of 100 men.
---> '''Princess Whats-Her-Name:''' Or a hundred man sized crows.
* WomenAreWiser: In the cartoon. Her comedic flaws and quirks are far more limited than the rest of the heroes, or most of the cast altogether (it ''is'' a nineties cartoon).

A strange creature that resembles a large blob of green goo with eyes, Snott is stated in the games to be a refugee from the Mucus Planet, having snuck into Jim's backpack in search of a sandwich while Jim was recovering after defeating Major Mucus. In the cartoon, his origins are never revealed.
* TheBlob
* CanonImmigrant: Though WordOfGod is that he was the slimy platforms that Jim battled the Queen on in the final level, for most people, he only appeared in the cartoons and was then brought to the games in ''Earthworm Jim 2''.
* EloquentInMyNativeTongue: To Jim and Peter, who can understand him, Snott can say a lot of things with only a few gibbers.
* TeamPet: His basic role in the cartoons seems to be this.
* TheUnintelligible: Snott speaks a language of slurps, gurgles and burbles.

'''Game:''' A notorious mercenary and bounty hunter, feared for his determination, Psycrow was commissioned by Queen Slug-for-a-Butt to bring her the Super Suit newly constructed by Professor Monkey-for-a-Head. But on the way, he was attacked by an agent deployed from the Phlegm Galaxy by Major Mucus, who stole the Suit. Psycrow destroyed the agent's ship, but the suit plunged to the planet Earth, creating Earthworm Jim. Determined not to fail his mission, Psycrow stalked Jim all the way to the planet Insectika, striving to ambush Jim on each leg of his journey between planets.

'''Cartoon:''' A nefarious space villain, Psycrow roams the galaxy in search of plunder and excitement. Conquest, destruction, thievery, these are all part of a day's work for a Scourge of the Spaceways. He's not above hiring himself out as a mercenary or assassin, but he's quite comfortable working for himself. Voiced by Creator/JimCummings.
* AbnormalAmmo: In the games, Psycrow's primary weapon is a gun that fires giant fish hooks.
* UsefulNotes/AmericanAccents: The cartoon gives Psycrow a "{{Joisey}}" accent.
* ArchEnemy: Queen Slug-For-A-Butt may be the BigBad, but Psycrow is Jim's primary foe in the games and often the cartoon, and with good reason. Not only is he a crow (and crows eat worms), but his WeaponOfChoice is [[HooksAndCrooks something many earthworms meet gruesome ends impaled on.]] He is like an amalgamation of earthworm nightmares.
* BountyHunter: But of course.
* CardCarryingVillain: While nearly all of the RoguesGallery display this trait in the cartoon, Psycrow seems to have the most devout professional life regarding it, with several of his intervals being mock-commercials for villainous societies or victims of superheroism.
* CreepyCrows: Ok, he's not as creepy as other examples, but he is villainous and his presence is bad news for our earthworm hero.
* {{Determinator}}: Psycrow doesn't give up easily.
* TheDragon: To Queen Slug-For-A-Butt on frequent occasions whom he sees as a profitable employer rather than as a master.
** Upgraded to full-on BigBad in the second game.
* FatBastard: Despite being a BountyHunter he is a bit out of shape if his pot belly is any indication.
* FeatheredFiend: No wonder he is Jim's arch-nemesis - crows (just like many other birds) are known for eating worms.
* TheHeavy: He is the antagonist most directly affiliated with the whole super-suit plot, as the [[BigBad BigBad's]] main agent and enforcer. That's why the plot of the whole game is one big chase between him and Jim, with every other enemy acting as an intermission to this.
* InSpaceEveryoneCanSeeYourFace: His fishbowl-like helmet is perfectly transparent.
* PunnyName: He's a villain whose name is a play on "Psycho" and crow and while not particularly unstable he is a sociopathic killer.
* RecurringBoss: In the first game.
** SkippableBoss: The player only has to fight him if they lose the "Andy Asteroids" race.
* SpellMyNameWithAnS: Is it Psycrow or Psy-Crow?
* WouldHurtAChild: Where other people may see cute little puppies that need tender care and affection, he sees perfect projectiles that can go splat and inconvenience his nemesis Jim.

[[folder:Evil the Cat]]
'''Game:''' Immortal incarnation of evil, this vile cat seeks to eliminate all that is good and wholesome in the universe, forever plotting and devising new methods of tormenting and corrupting from his nightmarish homeworld of Heck. His twin brother Flagitious runs the Circus of Scars, and during the summer, the two switch jobs.

'''Cartoon:''' Depraved ruler of Planet Heck, the source of all true evil, this mad feline yearns to destroy the universe. Voiced by Edward Hibbert.
* {{BFG}}: Never one to do things the simple way, he attacks Jim with an enormous gun that shoots fireballs.
* BornUnlucky: In the cartoon, Evil is perhaps more prone to AmusingInjuries than anyone else because of [[TheKlutz his own carelessness towards his environment]], usually has his plans thwarted by circumstances beyond his control, [[spoiler:and can't even keep romantic interests]].
* CardCarryingVillain: What would you expect from a cat who is the embodiment of pure evil?
* CatsAreMean: The core precept behind Evil the Cat.
* CatsHaveNineLives: You need to kill Evil nine times in the video game for him to finally bite the dust for good.
* DidntThinkThisThrough: In the cartoon, when questioned, by his own dumb thug no less, on what he plans to do in the aftermath of succeeding in destroying the entire universe, he admits he had never really thought about it. Doesn't stop him, though.
* EvenEvilHasLovedOnes: He unexpectedly developed feelings for both a cat that he saw on TV and for his soulmate Malice the Dog and doesn't seem to realise the paradox of hating anything good and yet having good feelings for another being. Heck, he isn't willing to admit that its love, despite his choice of words making it obvious.
* GlowingEyesOfDoom: He has glowing yellow eyes. During the second phase of his battle, [[ByTheLightsOfTheirEyes this becomes crucial to spot him when he tries to pounce you]].
* OmnicidalManiac: His cartoon version is obsessed with destroying the universe.
* PlanetHeck: Rules the {{Trope Namer|s}}.
* PlayingWithFire: Fire is his primary means of attack, be it the fireballs shot by his {{BFG}} or just fire waves from nowhere.
* {{Satan}}: In the games, he is basically this.
* TacticalSuicideBoss: Overlapping with BossArenaIdiocy. His own fireball gun destroys the platform he is perched on and allows Jim to recover his stolen suit. It fits with his careless behaviour.
* UnholyMatrimony: With [[DistaffCounterpart Malice the Dog]]. They hit it off rather well, until Malice left him to star in her own show in a different universe.
* WickedCultured: In the cartoons, he has the most hellish and destructively evil ambitions of the RoguesGallery, [[VillainsOutShopping spaced in between the odd movie night and romantic affair of course]].
-->'''Evil:''' I'm just watching TV.
* WouldHurtAChild: Upon learning that a little boy was the living embodiment of kindness and purity of heart, he became obsessed with destroying him exactly because of those qualities rather than in spite of them.

[[folder:Bob the Killer Goldfish (and #4)]]
'''Game:''' Upon a world that is almost entirely water and subaquatic mountains, Jim encounters Bob, a small fish with incredibly high intelligence. Always more intelligent than the other fish surrounding him, Bob finally dared to approach the surface, where he found the limited landmasses housed dimwitted but incredibly powerful humanoid felines. Awestruck by Bob's cowardly screams, they took him in as their god, giving him a fishbowl so that he could be carried around and guide them. Enslaving the stupid brutes, Bob used his intellect to help them construct a labyrinthine underwater city. Ambition flooding him, Bob desires to conquer the universe -- but what could a fish in a bowl do to achieve that goal? Jim's Super Suit is a way to compensate for his unimposing physique, and so he yearns to claim the suit for himself.

'''Cartoon:''' Fundamentally identical to the games, Bob in the cartoon series is depicted with a bombastic, evangelical personality. The planet is also given the name La Planeta de Agua (''[[RunningGag ¡arriba!]]''). Voiced by Creator/JimCummings.
* UsefulNotes/AmericanAccents: The cartoon gives Bob the particular take on the Dixie accent associated with fire-and-brimstone televangelist preachers, complete with mannerisms and method of speech.
* AntiClimaxBoss: In both games. In Down the Tubes, you just need to tip over his fishbowl. In Anything but Tangerines, Jim simply plucks him out of his fishbowl and eats him.
* BrainsEvilBrawnGood: [[ExaggeratedTrope Exaggerated]] and played for laughs. His relationship with Jim is a classic example of a powerful hero facing off against a physically weak villainous mastermind. His level is [[ThatOneLevel among the hardest in the whole game]], yet Bob himself is so physically weak that Jim can defeat him just by walking into him and toppling his bowl over.
* TheBrute: #4. In the games, he can't be fought, only avoided -- touch him and he'll send you flying.
* DumbMuscle: #4 is so dimwitted, he can be tricked to chasing a clockwork mouse that's been tied to his tail... but is strong enough that even Jim fears getting into melee with him.
* EvilutionaryBiologist: The episode "Darwin's Nightmare" revolves around Bob trying to "evolve" himself into a higher lifeform, recognizing that his fishy body is a physical limitation in his particular line of work.
** Comes complete with EvolutionaryLevels; Bob goes from a goldfish to a giant lungfish to a T-rex to a human to a human with a giant skull-piercing brain, super-intelligence & psychic powers and finally ends up a goldfish again.
* GeniusCripple: Bob is a scientific and architectural genius, handicapped by the fact that... well, he's a tiny little fish with no limbs. He's dependent entirely on the hands of his dimwitted feline minions.
* GoneHorriblyRight: In the cartoons, Bob's plans often turn up like this. Show them how to "destroy, destroy, destroy"? One of them attacks him in the same manner. Awaken the Anti-Fish? It not only refuses to obey him, but sets off to destroy the entire universe by eating the Great Worm Spirit, forcing Bob into an EnemyMine situation with Jim if he wants to be able to conquer the universe. Mutate the entire fish population of La Planeta de Agua ([[RunningGag ¡arriba!]]) to have super intelligence? They realize he is a dangerous megalomaniac who must be obliterated and turn on him.
* GratuitousSpanish: The cartoon name for Bob's world translates to "The Water Planet" or "The Planet of Water", and is always followed in show by a Mexican yell (usually written as "¡Arriba!").
* HarmlessVillain: In the games... sorta. He may be an AnticlimaxBoss, but his level is grueling and his minions are formidable fighters. In the cartoons, he is bumbling like all the others but can cause trouble for Jim in his schemes.
* {{Irony}}: In the cartoon, Bob's accent and mannerisms are a blatant ShoutOut to a Dixie televangelist, yet he is actually a hardcore scientist and easily comparable to Professor Monkey-for-a-Head in his genius.
* MobileFishbowl: He's a regular goldfish in a bowl who gets carried around by a musclebound cat minion.
* SilentAntagonist: #4 and, in the games, Bob.
* TomTheDarkLord: The most evil conqueror and destroyer fish in the universe is named Bob.

[[folder:Professor Monkey-For-A-Head]]
'''Game:''' A mad genius on retention to Queen Slug-for-a-Butt, the Professor works in a nightmarish laboratory space station, creating all manner of horrific creations. Working without restrictions, he indulges in all kinds of insane experiments, most notably an effort to improve his intelligence by grafting a monkey to his head. This backfired terribly; he now has double the headaches, the monkey continually messes up his experiments, and he can't get the shape of bananas out of his head. At the Queen's bidding for an ultra-powerful weapon to conquer the galaxy, he created the Super Suit -- and then the monkey ate the blueprints. This wouldn't have been such a bother if Psycrow hadn't gone and lost the Suit. When Jim wandered into his laboratory, the Professor eagerly tried to reclaim his handiwork, but failed.

'''Cartoon:''' A freelanced evil scientist, the Professor claims he was once a simple dairy farmer before he grafted a monkey to his head and became the most notorious of evil geniuses in the galaxy. Creator of the Super Suit, he can build another one whenever he wants with no problems -- but the suit requires a Battery of the Gods to function, and he only had the one. When he tried to get another, he was [[CoolAndUnusualPunishment turned into a human breadmaker]] and can, by focusing, produce warm fresh bread from inside his clothes. He notes this is actually kind of handy, as twisting the monkey's tail lets him make pumpernickel. Voiced by Creator/CharlieAdler.
* BerserkButton: In the cartoon, the Professor hates fruit carts because one killed his father. The manual for the first game mentions that calling him "Monkey Professor-For-A-Head" is a good way to make him angry.
* BodyHorror: It's PlayedForLaughs, of course, but still - the guy has a monkey surgically grafted to his head!
* DomesticAbuse: In the cartoon, the relationship between the professor and the monkey isn't exactly wine and roses. The monkey has accused the professor of "making eyes at a gorilla" (to which the professor angrily retorted he's a happily grafted man), viciously beaten him with a steel rod during a psycho-therapy session, and deliberately put shrinking cream in the professor's coffee so it can watch shows the professor hates.
* FromNobodyToNightmare: In the cartoon, it's claimed he was once nothing more than a simple dairy farmer. Now, he's such a space villain he has even been featured on Lifestyles Of the Rich & Villainous, with such creations as the Sparktronic Protobluntifier, the Atomic Banana, the Pay Toilet, and, of course, the creation of the Super Suit.
* MadScientist: You don't get much madder than attaching a monkey to your own head.
* MultipleHeadcase: Technically inverted, the Professor and the Monkey are more like two bodies sharing a head. But the two have separate brains, so there can still be dissension between them - such as the monkey eating the Prof's super suit plans.
* NoPlansNoPrototypeNoBackup: Subverted. In the games, the professor had blueprints but the monkey ate them, while in the cartoon, he can rebuild the suit whenever he wants, he just can't make them as powerful without another Battery of the Gods.
* StrawCharacter - Scientist: According to his blog, the creationist Creator/DougTenNapel created Professor Monkey-for-a-Head as a TakeThat to his Darwinist high school science teachers. The cartoon transferred this trait to Bob.

[[folder:Queen Pulsating Bloated Festering Sweaty Pus-Filled Malformed Slug-for-a-Butt]]
Horrific ruler of the planet Insectica, the Queen reigns over all with a set of iron fists and she yearns to expand her dominion to the entire universe. Commissioning the creation of an ultra-powerful doomsday weapon from Professor Monkey-for-a-Head, the Queen was infuriated to learn the weapon had been lost. She seeks to destroy Earthworm Jim whenever possible as she hopes to reclaim the Super Suit, but she does not focus maniacally on that goal and will try to rule the universe in other ways. Voiced in the cartoon by Andrea Martin.
* AssKicksYou: Her formidable butt is an enemy in and of its own, and must be fought and destroyed to reach her body. And even after the tip of the butt is beaten, Jim still needs to deal with repugnant secretions dripping down from the rest of it.
* AuthorityEqualsAsskicking: She is the queen of Insectika, and both ends of her body can kill you. She is even more powerful while wearing the Super Suit.
* BeautyIsBad: By the standards of her people, the queen is considered incredibly attractive. Yet rules her planet like a tyrant
* BigBad: Final boss of the game, and typically portrayed as the most important of Jim's RoguesGallery in the cartoons.
* BigCreepyCrawlies: Her design draws some clear inspiration from termite queens.
* EvilOverlord: Albeit she's not very good at it.
* FatBastard: While her main body is pretty much skeletal, her butt... well...
* GodSaveUsFromTheQueen: Somewhat downplayed in the cartoons, where the Princess explains they need to reinstate Queen Slug-for-a-Butt after Psycrow and the Professor depose her because she's such a lousy evil queen that she never actually manages to conquer anything. She is only a pain for her subjects.
* GoodWingsEvilWings: The Queen has insect wings, but they're so tiny in proportion to her body (mostly to her butt) that she is pretty clearly incapable of flying.
* InsectQueen: Naturally. And since she is meant to be an alien and monstrous example invoked for horror, she rules the insectoid aliens as both their Queen and their [[MonsterIsAMommy mother]].
* MagicWand: Her scepter.
* MeaningfulName: Almost to the point of ExactlyWhatItSaysOnTheTin.
* OddFriendship: With Jim's NosyNeighbor Mrs. Bleveredge in "The Exile of Lucy" and "Hyper Psy-Crow".
* OverlyLongName: Overly long and overly accurately gross are both fitting ways to characterise it.
* TurtleIsland: An unusual example. In the games, her rear is so insanely huge, a good part of the final level takes place on or around it.

Evil the Cat's #1 lackey, the masochistic Henchrat lives to obey Evil's every command, thanking him with genuine delight for his abuse. When not on the clock, Henchrat enjoys jumping up and down on hats... while people are still wearing them. Voiced by John Kassir.
* AbnormalAmmo: The Henchrat's choice of weapons consist of exploding mushy cheese grenades and a gun that fires quick-setting melted cheese.
* TheBrute: Henchrat serves as the muscle to Evil's brain, and is quite happy with this state of affairs.
* CanonForeigner: Only introduced in the cartoon series.
** CanonImmigrant: He later appeared with Evil in ''Menace 2 The Galaxy''.
* HiddenDepths: Henchrat enjoys macrame, watching mimes, playing piano and making balloon animals.
* YouNoTakeCandle: His general method of speech.

[[folder:Evil Jim]]
A twisted opposite of Jim spawned when a distorted photo-copied image of Jim was exposed to toxic waste, Evil Jim yearns to destroy his heroic counterpart. Voiced by Creator/DanCastellaneta.
* AllGirlsWantBadBoys: In "The Anti-Fish," Evil Jim does a better job of wooing the Princess than Jim ever did.
* CanonForeigner: Created for the cartoon series.
** CanonImmigrant: He appeared in the tie in game ''Menace 2 The Galaxy'' however and was planned to appear in ''3D''.
* EvilTwin
* IJustWantToHaveFriends: In one episode, Evil Jim gets jealous of Jim's friends and decides to make clones of them for himself. They naturally end up being the exact opposite of the originals in personality.
* IronicEcho: Evil Jim states that everything Jim loves, he hates, and vice-versa. However, Jim points out that since he hates losing, Evil Jim must love it.
-->'''Evil Jim:''' Don't be so literal-minded. ''(proceeds to beat up Jim)''
* WickedCultured: Evil Jim can be very eloquent and mature, when he's not focused on mayhem and destruction.

[[folder:Evil Princess Whats-Her-Name]]
One of Evil Jim's attempt to clone himself some evil companions/minions, using a cloning ray to create an evil duplicate of the insectikan princess. Only to realise to late that the opposite of an ActionGirl is not as useful as he thought she would be.
* BeautyEqualsGoodness: Inverted. Though she is still ugly by her people standards.
* BrainlessBeauty: Funnily enough. The evil clone of the Princess acts more like she did in the game than in the cartoon.
* EvilKnockoff: What she was created for, but leaves much to be desired. Evil Jim lampshades the usefulness of making an opposite personality clone of a mighty warrior woman.
* GirlyGirl: Dresses, speaks and acts more more femininly than her original counterpart
* GracefulLadiesLikePurple
* IBrokeANail: Makes this complaint when using her own gun.
* ImpossibleHourglassFigure
* NonMammalianHair[=/=]RapunzelHair
* NonMammalMammaries
* ObliviousToLove: Inverted. While normal What's-Her-Name can't take a hint or denies Jim affections, Evil Princess has no problem asking Jim out on a date much to his shock.
* VanityIsFeminine: Never goes anywhere without her makeup. Demands someone call a hairdresser after getting in a bike crash, which she got in because she was trying to fix her make-up while riding.