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!Doctor Horrible/Billy

-->''"The world is a '''mess''', and I just...need to rule it."''

* {{Adorkable}}: He can't talk to girls, he thinks that Captain Hammer and Penny are "probably going to French kiss or something" and is played by Creator/NeilPatrickHarris.
%%* AffablyEvil
%%* AntiVillain
%%* BadassBookworm
* BewareTheNiceOnes: He has genuinely good intentions [[WellIntentionedExtremist for both the world]] and [[DoggedNiceGuy in his friendship with Penny]], but ends up being pushed far enough by Captain Hammer for him to [[spoiler: try to]] break his rule of ThouShaltNotKill.
* CannotTalkToWomen: Mostly because of how painfully, painfully shy he is.
%%* CardCarryingVillain
* ClarkKenting: Dr. Horrible's outfit is Billy in a labcoat and goggles. Backfires on him [[spoiler: when Captain Hammer recognizes Billy at first glance.]]
* DeadpanSnarker: "Wow, sarcasm. That's original."
%%* EvenEvilHasStandards: Especially at first.
* EverythingButTheGirl: [[spoiler:Billy gets money, fame and acceptance into the Evil League of Evil but only because the girl he loves dies.]]
* EvilCostumeSwitch: In act I, when he changes in his Dr. Horrible outfit. [[spoiler: And at the end of act III, when he dons his new {{red and black|And Evil All Over}} costume.]]
* EvilLaugh: He has a voice coach for it. By act III, he's finally got it.
%%* FromNobodyToNightmare
* GogglesDoNothing: He wears a pair, but they don't seem to serve any sort of purpose other than [[RuleOfCool looking cool]].
* HeroWorshipper: {{Inverted}}. He evidently has deep admiration for Bad Horse, which Penny finds disturbing when he accidentally mentions it.
%%* IneffectualSympatheticVillain: [[spoiler: Until the end.]]
* JumpingOffTheSlipperySlope: Or SlowlySlippingIntoEvil in this case over the course of the musical.
%%* LonelyAtTheTop
* LooseLips: Turns out Captain Hammer and the LAPD are among his listeners.
* LoveMakesYouEvil: In a strange sort of way because Penny was also his MoralityPet. [[spoiler: When she died, so did his conscience.]]
%%* MadScientist
* MorallyAmbiguousDoctorate: He has a [=PhD=] in horribleness!
%%* NeverHurtAnInnocent
%%* NobleDemon: [[spoiler: At least until the end.]]
%%* NoRespectGuy
%%* NotSoHarmlessVillain
* PsychoticSmirk: When he starts singing ''Brand New Day''.
%%* SanitySlippage
%%* ShrinkingViolet
* StalkerWithACrush: To Penny.
%%* StepfordSmiler: [[spoiler: After Penny dies.]]
%%* SympatheticPOV
%%* TechnicalPacifist
%%* TenorBoy
* ThouShaltNotKill: Personally against murder, which he sees as beneath him--or at least, would like to. It seems to be implied that the ''real'' reason he won't kill is because he knows it's wrong, but he doesn't wanna admit that, so makes up an excuse about it not being "elegant or creative" enough.
%%* TwitchyEye
%%* VillainProtagonist
* WellIntentionedExtremist : He's vague on the specifics, but he clearly thinks that the world would be a better place with him in control.
* WhatTheHellHero: He calls out Captain Hammer for pushing Penny out of the van's way and into some trash.
* WoobieDestroyerOfWorlds: [[spoiler:By the end of the musical.]]

!Captain Hammer
-->''"Everyone's a hero in their own way/You and you, and mostly me, and you."''

%%* AntiHero
%%* BadassBaritone
* BigBad: People think of him as a hero, but he screws with Doctor Horrible for the hell of it which forms the conflict.
* BitchInSheepsClothing: Despite being a self-proclaimed hero, he's quite the jerk.
%%* CaptainSuperhero
* ChestInsignia: Of... well, guess.
* CivvieSpandex: His superhero outfit is mostly just normal articles of clothing.
* CreateYourOwnVillain: If he hadn't been so convinced that all nerdy kids are potential supervillians, he probably wouldn't have to worry about Dr. Horrible.
* DropTheHammer: Averted; despite having the word "hammer" in his name, he's never actually seen using one as a weapon.
* DumbIsGood: Advocates anti-intellectualism, telling kids that any kid who's either a nerd or "different" in some ways is a potential supervillain who must be put behind bars.
* DumbMuscle: Downplayed in that he's smart enough to follow Dr. Horrible's Blog and [[spoiler: immediately realizes that Billy is Dr. Horrible.]]
%%* FakeUltimateHero
* GoodIsNotNice: He's a petty, bullying, anti-intellectual, womanizing smug jerk. He also saves the city from super-villains on a regular basis.
%%* HandsomeLech
* HaveIMentionedIAmSexuallyActiveToday: "So I thank my girlfriend Penny (yeah, we totally had sex)/She showed me there's so many/Different muscles I can flex..."
%%* HeroAntagonist
%%* HeteronormativeCrusader
* IWantMyMommy: "Mama! Someone maternal!"
* JerkWithAHeartOfJerk: Penny thinks Captain Hammer is really sweet on the inside, despite being [[TheAce a bit full of himself]]. The truth is Captain Hammer is only being nice to Penny to get in her pants and ''simultaneously'' piss off the eponymous character. Lampshaded when Penny states that he must have a deeper layer that's totally different from the one on the surface, Bily replies that he sometimes there's a third, even deeper level, and that one's exactly the same as the top surface level -- like pie.
* KickTheDog: He dates and sleeps with Penny just to spite Billy.
* KnightTemplar: Full of himself, nonetheless.
%%* MadeOfIron
%%* AManIsNotAVirgin
* MeaningfulName: [[spoiler:The hammer is his penis.]]
* MilesGloriosus: He's only brave and bold because he's invulnerable to damage. The first time he gets hurt, he literally runs screaming like a little girl and spends months in therapy over it.
%%* {{Narcissist}}
* NeatFreak: Why he constantly wears gloves. Pay attention to him during the "My Eyes" song and it becomes clear he's hiding it from Penny. Possibly just an extension of his superiority issues over everyone else.
%%* NighInvulnerability
* NonIndicativeName: For a guy named Captain Hammer, he's never once seen wielding a hammer.
%%* NotSoInvincibleAfterAll
* PoliticallyIncorrectVillain: "I hate the homeless...[[LastSecondWordSwap ness problem]]."
%%* {{Pride}}
* ReallyGetsAround: Has never slept with the same woman twice.
* ScienceIsBad: In the spinoff comic, he demands that children report nerds to the police.
* SmugSnake: His ego's just a ''bit'' too big for his own good.
%%* SmugSuper
%%* VillainWithGoodPublicity

-->''"Even in the darkness/Every color can be found..."''

%%* DiesWideOpen
%%* HeroesWantRedheads
%%* TheIngenue
* KillTheCutie: [[spoiler:Her ultimate fate. Of course, given that it's a Joss Whedon production, many author-savvy viewers saw it coming.]]
%%* LoveMartyr
* MeaningfulName: Lots of possibilities. It could refer to her charity and optimism, her being something under valued or unimportant, or [[spoiler:a payment for what Doctor Horrible wants in life.]]
%%* MoralityChain: For Dr. Horrible.
%%* NiceGirl: Her defining trait.
* NotListeningToMeAreYou: Her speech to Billy in act I trails off into "...so they can buy rocket packs and go to the moon... and become florists" when she sees that he's not paying any attention.
* ParentalAbandonment: Implied in her prequel comic.
* StoodUp: She waits for Billy in act III with frozen yogurt, but he was busy plotting Captain Hammer's murder.
%%* TooGoodForThisSinfulEarth
%%* TrashLanding
%%* WideEyedIdealist

-->''"Look at me, man. I'm Moist. At my most badass, I make people feel like they want to take a shower."''

* AntiVillain: To some extent, though he seems to have less of a problem than Billy when it comes to killing someone.
* TheDragon: To the good doctor himself, sorta.
* BlessedWithSuck: His "Power" is merely that he's perpetually moist.
* MinionWithAnFInEvil[=/=]IneffectualSympatheticVillain: Played with, given that his boss is actually ''worse'' at the whole evil gig.
* MisappliedPhlebotinum: His condition is due to a plutonium-powered air moistener that his father got to fix his dry skin.
%%* PowerIncontinence
* WhatKindOfLamePowerIsHeartAnyway: The power of moistening is... not the greatest.
* WeirdTradeUnion: The Henchman's union.

!Bad Horse
-->''"[[YouHaveFailedMe There will be blood, it might be yours]]\\
So go kill someone\\
Signed, Bad Horse"''

* AnimalSuperheroes: {{Inverted}}: he is a horse, and he's evil.
* AnimalThemedSuperbeing: Until the reveal that... he really ''is'' a horse.
%%* BiggerBad: See SmallRoleBigImpact below.
%%* CoolHorse
* TheDreaded: Bad Horse inspires equal parts dread and awe from other villains. And only the former from everyone else.
* TheDon: Based on his letters, he's this to the Evil League of Evil.
* EvilLaugh: His terrible death whinny. It apparently set the standard for evil laughs.
* ExactlyWhatItSaysOnTheTin: He's a horse, and he's bad.
%%* HellishHorse
* InSeriesNickname: ''The Thoroughbred Of Sin''
%%* LivingLegend
* SmallRoleBigImpact: Though he only has a seconds-long cameo at the very end of the video, Bad Horse's high standards for joining the Evil League of Evil is what drives Dr. Horrible to build his freeze ray and set off the plot.
%%* SuperheroSobriquets
* TheUnintelligible: Subverted. Speaks only in horse sounds for most of the ELE comic, with Jefferson claiming that Bad Horse was appointing him "vice president." Subverted at the very end when Bad Horse speaks English.

![[DepartmentOfRedundancyDepartment Evil League of Evil]] leaders

* '''Professor Normal''': A cyborg with a super strong robotic arm and jaw attachment. Otherwise, he's a normal person with a wife, kids, and a mortgage, hence his name.
* '''Tie-Die''': A hippie-themed supervillainess able to make hippies violent and angry.
* '''Dead Bowie''': A Music/DavidBowie-inspired supervillain with the power of persuasion. Claims to be the real David Bowie who was murdered in 1980 and that every other appearance by Bowie afterward was an impostor.
* '''Fury Leika''': Her name is a [[PunnyName pun]] off of "Hell hath no fury like a WomanScorned." She was left at the altar and then pooped on by a radioactive dove. She gained poisonous flowers, incendiary rice, a ball and chain around her ankle, and four henchwomen each carrying something old, something new, something borrowed, and something blue.
* '''Fake UsefulNotes/ThomasJefferson''': Claims to be the real Thomas Jefferson, though that is unlikely unless Jefferson had SuperStrength. Also carries a quill pen tipped with tranquilizer. Doesn't like to have lots of stuff in his pockets.
* '''Snake Bite''': A supervillainess with a cobra-themed costume. Carries a staff topped by a mini disco ball. Did not appear in the "Evil League of Evil" comic with the other ELE leaders.

!Minor villains
* '''Hourglass''': A villainess who is able to see the future. Tells Moist about a kid who will become President. An [[TheGhost unseen character]].
* '''Bait and Switch''': A pair of villains who Moist goes on a double date with. Somehow able to fool him into thinking he was dating [[PunnyName Bait instead of Switch]]. Bait is apparently the more attractive of the two.
* '''Conflict Diamond''': A villain friend of Dr. Horrible and Moist. Went on the double date with Moist, Bait, and Switch.
* '''Pink Pummeler''': A [[ArmoredClosetGay homophobic villain]] with SuperStrength, {{Gaydar}}, and a superhuman knowledge of Broadway show tunes. Repeatedly rejected by the ELE due to his tendency to date rather than beat up his victims.
* '''Purple Pimp''': A supervillain who appears at the party for Dr. Horrible's induction into the ELE.

!Minor heroes
* '''Johnny Snow''': An amateur hero not affiliated with the Council of Champions. He wears a parka and carries an [[AnIcePerson Ice Beam]]. He's mentioned in Act I and appears in the ELE comics, where he foils a plot by the ELE to poison the city's water supply by [[NiceJobBreakingItHero freezing it]]. The villains are [[YourApprovalFillsMeWithShame impressed]] and mistake him for a supervillain.
* '''James Flames''': A potbellied superhero with [[PlayingWithFire pyrokinetic]] powers. His CatchPhrase is "M'name's James and I have the power of flames." Wears a singed T-shirt and pink overalls. He thaws the water supply after Johnny Snow freezes it.
* '''Wingspan''': A member of the Council of Champions, along with Captain Hammer. Works in a command center in the sky. He, along with the rest of the Council, foolishly leaves the city for a retreat, allowing the ELE to wreak havoc. Appears briefly in the ELE comic.
* '''Elementia''': A blue-skinned superheroine and a member of the Council of Champions. Works in a command center with a waterfall and apparently has some kind of ElementalPowers. Has no problem sitting naked in a sauna with men.
* '''???''': An unnamed member of the Council of Champions with a third eye in the middle of his forehead. He appears in the sauna with the rest in the ELE comic.