!Batman[=/=]Bruce Wayne
!!Voiced by: Creator/BruceGreenwood
%%* TheAtoner: Following Jason's death.
%%* BadassBaritone: Courtesy of Bruce Greenwood.
* TheFettered: Unlike Red Hood. He states he's been tempted to kill the Joker, many, many times, but he's afraid that if he starts killing he will [[HeWhoFightsMonsters take a step too far and never stop]].
%%* GeniusBruiser
* HeartbrokenBadass: A non-romantic version. Jason's death and [[spoiler: subsequent downfall into villainy]] left him broken and angry at himself for not being able to save Jason and leaving him with the wish that he could have done more for him if he had the chance.
* MyGreatestFailure: Jason's death and his downfall into becoming [[spoiler: Red Hood]].
* SaveTheVillain: Saves Red Hood more than once throughout the film. [[spoiler: His moral code is so strong that even saved the Joker.]]

!Nightwing[=/=]Dick Grayson
!!Voiced by: Creator/NeilPatrickHarris

* ButtMonkey: Batman keeps leaving him behind.
* DeadpanSnarker: "I'm chatty. It's part of my charm."
* GameBreakingInjury: Injuring his leg while he and Batman chase after Red Hood both [[ShooOutTheClowns writes him out of the story]] and [[MythologyGag references him wearing a brace on his leg in the original comic.]]
%%* SidekickGraduationsStick

!Robin II[=/=]Jason Todd
!!Voiced by: Creator/VincentMartella

* AntiHero: Flashbacks show him more willing to injure people than Batman would like.
* DeadSidekick: He's killed by the Joker in the opening.
* LeotardOfPower: In his early Robin days.
* PosthumousCharacter: The story opens with his murder by the Joker.
* TimeShiftedActor: Young Jason is voiced by Alexander Martella. [[spoiler: And then there's [[Creator/JensenAckles Jason as Red Hood]].]]

!Alfred Pennyworth
!!Voiced by: Jim Piddock

%%* DeadpanSnarker: As per usual.
* NotSoStoic: Alfred is in top form, [[OnlySaneMan unflappable in the face of everything Batman-related]]. But when the computer confirms that [[spoiler:the Red Hood is Jason]], he drops the tray he was carrying, and can only stare at the screen in numb recognition.
%%* ServileSnarker

!Red Hood[=/=][[spoiler:Jason Todd]]
!!Voiced by: Creator/JensenAckles

* AMillionIsAStatistic: [[spoiler:The crux of Jason's grievance with Batman. Despite the "entire graveyards" worth of people that the Joker has killed, Jason was hoping he'd be special, that he'd be the one Batman actually avenged]].
* AntiVillain: His first act upon assuming control of the various gangs is to make children untouchable to the drug trade, and he only ever targets criminals in the course of his various activities. As he tells Batman during their first real chat, he's actively attempting to control the criminal element of Gotham, but we don't find out ''why'' until the climax.
* AsskickingEqualsAuthority: He sets himself up as the drug kingpin in Gotham and rival to Black Mask by showing up at a meeting of the [[JustForPun "heads"]] of the organizations under Mask's thumb and giving them a bag full of the heads of their lieutenants.
-->'''Hood:''' I did that in two hours. [[BadassBoast Wanna see what I can do with a whole evening?]]
* BackFromTheDead: [[spoiler: Courtesy of Ra's al Ghul and the Lazarus Pit.]]
* BigBadEnsemble: With the Joker.
* CameBackWrong: According to Ra's al Ghul. [[spoiler:The man himself throws it back in Batman's face, suggesting his current activity is somehow easier to digest if the Lazarus Pit "turned [him] rabid"]].
* TheChessmaster: [[spoiler: The movie's entire events were all a plan to get the Joker out of Arkham and put him in a situation where Batman would have no choice but to kill him.]]
* ColdSniper: He kills three people from a significant distance without touching Batman or Nightwing standing to either side of them.
* CombatPragmatist: Though he has plenty of gadgets up his sleeves, he's not afraid to use anything in the environment to his advantage in a fight, and, as noted under TheUnfettered, is willing to use ''any'' methods necessary to gain an advantage.
* ComplexityAddiction: His ultimate plan to [[spoiler:get Batman to realize that sometimes MurderIsTheBestSolution, specifically regarding irredeemable people like The Joker]]. Instead of just [[spoiler:finishing The Joker off himself and using that as the ultimate symbol of his new pragmatic approach being more effective than Batman's idealistic approach]], he goes to extra trouble to [[spoiler:get Batman to abandon his ThouShaltNotKill rule and finish The Joker off.]]
* CoolHelmet: [[spoiler:It explodes too]].
* CrazyPrepared: To be expected, as a member of [[spoiler:the Bat Family]].
* FauxAffablyEvil: He has a rather [[DeadpanSnarker snarky, personable, and somewhat laid back personality]], is cool under fire and logical with always a few tricks up his sleeves and sharp tongue to match. However, this belies his true nature of an angry, violent, young man with serious emotional and (possible) mental issues.
* GunsAkimbo: Likes to to use a pair of handguns.
* TheHeavy: The main antagonist and the one who drives the plot along for the majority of the film.
* HeWhoFightsMonsters: He's vigilante with no hesitation against killing, and his entire plan consisted of forcing himself into the criminal underworld to keep Gotham's criminals in line.
* {{Irony}}: There's the fact that his actions are based around heroism, but are so brutal that he is a firm villain. But there is also a bit of symbolic irony in [[spoiler: a bat family member becoming a ''new'' Red Hood, with the original being the original villain alias of who else but The Joker.]]
* KnifeNut: After the guns, before the fists, he has a knife that can cut Batman's cables.
* KnightTemplar: Believes that everything he has done, every life he has taken, has been in the name of bringing order and stability to Gotham.
-->[To Batman] "Yes. You can't stop crime. That's what you never understood. I'm controlling it. You wanna rule them by fear. But what do you do with the ones who aren't afraid? I'm doing what you won't, I'm taking them out!"
* NotSoDifferent:
** To Batman; A [[VigilanteMan masked vigilante]] with a penchant for the StealthHiBye, who preys on criminals and develops a [[TheDreaded reputation of dread]], a [[WalkingArmory small arsenal of various weapons and explosives on his person at all times]], listening devices installed in Black Mask's office, an expert marksman with a penchant for firearms instead of thrown weapons, even a grappling line that sees use in their final battle; Really, the only thing that differentiates the two is Hood's willingness to kill. [[spoiler:This is the first big clue as to his identity]].
** To the Joker; Aside from assuming his old alter-ego, [[spoiler:he captures the Joker, beats him with a crowbar, and then tries to finish him off in a building rigged to explode]].
* TheReveal: Blood analysis shows that he's [[spoiler:Jason Todd.]]
* TheUnfettered: Will use whatever methods necessary, no matter how brutal or violent, to achieve his end goal of "cleaning up Gotham." Despite that, [[EvenEvilHasStandards he still won't tolerate drug dealing to children, even if it would increase his cut of the profits.]]
* WoobieDestroyerOfWorlds:[[spoiler:He was once the partner of Batman and a former juvenile delinquent whose hard background, brutal death at the hands of the Joker, and subsequent resurrection drove him over the edge to become the murderous KnightTemplar Red Hood.]]
* WouldNotHurtAChild: Threaten to kill any of the drug lords if they sold drugs to children.

!!Voiced by: Creator/JohnDiMaggio

* AdaptationalBadass: In most adaptations, Joker is more of a schemer than a fighter, or at best a desperate scrapper when cornered. Here, he quickly and effortlessly murders a room full of armed mob bodyguards while ''handcuffed''. He's got a sturdier build than he is usually portrayed with, and [[TheBrute seems more capable of getting his own hands dirty]].
* AxCrazy: As usual. The movie ''opens'' with him beating Jason Todd with a crowbar. ''Almost'' to death. Then the bombs finish it.
--> "[[PreMortemOneLiner May I have some water]]?"
* BigBadEnsemble: With Red Hood
* DarkerAndEdgier: Kind of; this version of The Joker is more thuggish, but does the [[Film/KissOfDeath Tommy Udo]] version of the GigglingVillain.
* DeadpanSnarker: His humor and delivery are more sarcastic and measured than his usual bonkers antics. He still giggles like a deranged schoolgirl, but less frequently than many adaptations.
* EvilIsHammy: This aspect of him hasn't changed.
* EvilIsNotAToy: [[spoiler: Black Mask in a moment of desperation, breaks Joker out of Arkham in exchange for him killing the Red Hood. Naturally the Joker betrays him almost immediately.]]
* EvilSoundsDeep: Highly unusual for the Joker, but this plays into his DarkerAndEdgier characterization.
* HateSink: The Joker from ''WesternAnimation/BatmanTheAnimatedSeries'' was a monster to be sure, but his despicableness was counterbalanced by a [[LargeHam bombastic sense of showmanship]] and [[LaughablyEvil Comic Relief]]. Conversely, this Joker is less cartoonish and much more thuggish, despicable and realistically monstrous than usual.
* TheHeavy: In the greater scope; The events of this movie are entirely his fault, and he doesn't feel a ''drop'' of remorse for it.
* KarmaHoudini: [[spoiler: At the end of the day he suffered no comeuppance for his villainy, and he had an utter blast watching Batman's struggle with Red Hood.]]
* MonsterClown: Moreso than other incarnations.
* PretenderDiss: Says his time as the Red Hood was "Classy, more flashy ''maître d' ''than motorcycle fetish" when initially questioned by Batman and Nightwing, and [[CallBack later to Red Hood himself]].
* {{Sadist}}: He takes immense joy out of the suffering he inflicts on others.
* TheSociopath: He has absolutely no regard for human life, including his own.
* WouldHurtAChild: His ''first scene'' shows the Joker beating a teenage boy nearly to death, and then finishing the job with a bomb.
--> "Now, that was rude. The first boy blunder had some manners. I suppose I'm going to have to teach you a lesson so you can better follow in his footsteps. ({{beat}}) Nah, I'm just gonna keep beating you with this crowbar."

!Black Mask
!!Voiced by: Creator/WadeWilliams

* BadBoss: When ranting about Red Hood, he's willing to randomly punch his subordinates, notably the new guy who isn't used to [[RedRightHand his appearance]].
--> '''Black Mask:''' ''*suddenly calm*'' You. New guy. [[FalseReassurance Don't be nervous kid]], but if you keep staring at me like that, I'm gonna cut your eyes out.
--> ''[[{{Beat}} *New Guy tries and fails to look away*]]''
--> ''*Black Mask lays him out*''
* BigBadWannabe: Prior to its theft by Red Hood, AMAZO was to be Mask's ticket into international arms deals, a step-up from his current "bottom-feeding garbage" of small scale drug and weapons trafficking. Later, after a direct attack from Red Hood, Mask decides to get out of the "cross-fire" by [[spoiler:breaking the Joker out of Arkham, which goes about as well as could be expected]].
* CaptainObvious: During a rant about taking down the Red Hood.
-->'''Mask:''' [[LampshadeHanging Maybe my last request was too mired in subtlety.]] I want this man dead. When I say "dead", I mean "seriously ''dead''". Beaten. Broken! ''HIS-HEAD-MOUNTED-ON-MY-WALL'' KINDA DEAD!
-->'''Ms. Li:''' Understood. We'll be taking further precautions at every transaction.
-->'''Mask:''' Screw that! Time he learned that this is a contact sport. We're going on offense. Rough up ''his'' business; something big! Something ''loud''! When he shows up to shut us down, have a party waiting for him! And when I say "party" I actually mean a '''whole lot of people'' who are gonna KILL HIM!
-->'''Li:''' I figured.
-->'''Mask:''' Just being clear.
* EvenEvilHasStandards: He's clearly uncomfortable with hiring the Joker. [[ChronicBackstabbingDisorder He has good reason to feel that way.]]
* GenreBlind: He trusted the Joker. It doesn't get much worse than that.
** On the other hand, [[EvenEvilHasStandards Black Mask didn't ''want'' to hire the Joker]], and only did so out of desperation.
* HairTriggerTemper: Prone to beating up his subordinates for little to no reason. Though, to his credit, he is less homicidal than the comic version.
* LargeHam: His mannerisms are based on Creator/AlPacino in full overblown gangster mode.
* UnwittingPawn: Of the Red Hood; Black Mask is the only one with the connections to [[spoiler:spring Joker from Arkham, which was Red Hood's real goal all along]].

!Ms. Li
!!Voiced by: Creator/KellyHu

* DeadpanSnarker: Emphasis on deadpan.
-->'''Ms. Li''': [[MythologyGag Yes, Batman likes to keep things.]]
* TheDragon: To Black Mask. She doesn't do a lot onscreen, but she's apparently the one who organizes Black Mask's heists and plans out said heist's finer details.
* GenderFlip: Mr. Li in the comic.
* NotSoStoic: She loses her composure [[spoiler:while the Joker is holding her, Black Mask, and several drug dealers hostage, and is preparing to set them on fire]].
* NumberTwo: To Black Mask.
* OnlySaneMan: Black Mask is too enraged to think straight and everyone else is too scared of their enraged boss to be productive.
* SparedByTheAdaptation: Mr. Li is killed by Jason in the original story, whereas Ms. Li is fine by the end of the movie.
* TheStoic: Takes Black Mask's tantrums without so much of a change on her face.

!Ra's al Ghul
!!Voiced by: Creator/JasonIsaacs

* AffablyEvil: He states that he never resorts to violence or killing unless it's for a "greater cause" and is deeply remorseful that [[spoiler:his hiring of the Joker was ultimately what lead to Jason's murder; his attempt to fix things via Lazarus Pit resurrection is what sets off the main plot.]]
* TheAtoner: [[spoiler:After Jason's death, Ra's chooses not to oppose Batman again, and tries to resurrect Jason to try and repair the damage he inadvertantly caused]].
%%* BeardOfEvil
* EvenEvilHasStandards: He is greatly upset [[spoiler: by the death of Jason,]] calling it both unnecessary and brutal. Batman [[LampshadeHanging points out]] he never had a problem killing before. Ra's counters by pointing out he has [[KnightTemplar killed for a greater good]], never [[ForTheEvulz for laughs]] as Joker did.
* MyGodWhatHaveIDone: After his actions cause [[spoiler:Jason's death]], and again, after hearing of [[spoiler:Jason's actions as the Red Hood, following Ra's raising him from the dead]].
* UnwittingInstigatorOfDoom: [[spoiler:Twice. First, when his hiring of the Joker was ultimately what lead to Jason's murder. Second, when he did try to resurrect Jason to try and repair the damage he inadvertantly caused, but his actions [[CameBackWrong only made things worse]]]].