* Used in the ImagineSpot scene in the ''WesternAnimation/{{Arthur}} epsisode: "D.W. The Pickey Eater". Arthur was not allowed to go to outer space without her sister D.W.
* Used in the ''WesternAnimation/TeenTitans'' episode "Birthmark" by Raven when Slade shows her a vision of a destroyed world that she's going to create. Also used by Raven in the same episode when she refuses for a final time the party the others have planned for her birthday and destroys everything with her powers.
* ''WesternAnimation/MuchaLucha'' is a perfect example of this. The Flea did one when his baby sister Pulgita is about to be killed by The Masked Toilet so he went inside the toilet and Pulgita beats up The Masked Toilet and at the end of the episode The Flea did it again when his scary story when he told his baby sister who he tucked her in became true when The Masked Toilet wanted his shoes and The Flea said it three times.
** In ''The Return of El Malefico'', Buena Girl say this and she was too late because of Rikochet read the code of masked wrestling backwards which set El Malefico free. Also it was The Flea's fault because he tricked Rikochet into doing it while The Flea is controlled by El Malefico and at the end of the movie El Malefico also said this because he failed to take over the world.
* ''WesternAnimation/ThePenguinsOfMadagascar'' are in love with this trope. Most come from Skipper.
* ''Franchise/{{LEGO}} WesternAnimation/{{Ninjago}}: Masters of Spinjitsu'': Said by Zane when he [[RoboticReveal discovers that he is a robot]].
* ''WesternAnimation/SpongeBobSquarePants'': Used a lot in this show, for example...:
** Plankton lets one out at the end of "Plankton!" after he ends up stuck in his computer wife Karren.
** [=SpongeBob=] does this in "Pickles!" when he finds out he forgot the pickles.
** ""Nature Pants":
-->'''SpongeBob''' Patrick, I'm not coming home.\\
'''Patrick:''' I miss you! Sandy misses you! Even Squidward misses you! (Squidward is throwing himself a party at home)\\
'''SpongeBob:''' I'm happy here. This is my home now. (Sandy drags Patrick away)\\
'''Patrick:''' Nooooo!! [=SpongeBob=]!!
** Squidward does one in "Opposite Day" when he sees Patty's car.
** Mr. Krabs lets one out in "F.U.N", when Plankton steals the formula.
** Employee of the Month: "NOOOO! How could I have let the quality of my work slip so much!"
** "Sleepy Time":
-->'''[=SpongeBob=]:''' Mrs. Puff! Look! I've finally got my driver's license!\\
'''Mrs. Puff:''' (takes his license and tears it up) Not even in your dreams, Mr. [=SquarePants=]!\\
'''[=SpongeBob=]:''' Noooo!
** In "Something Smells":
-->'''Patrick:''' Maybe it's just because you're ugly.\\
'''[=SpongeBob=]:''' Ugly?! ''(sticks finger in mouth, slides it across his eyebrows)'' You gotta be kiddin' meh.\\
'''Patrick:''' Let us try the reflection test. ''(holds up a mirror in front of SpongeBob)''\\
'''[=SpongeBob=]:''' ''(to reflection)'' Hi! ''([=SpongeBob=]'s reflection grabs a hammer and breaks itself after smelling [=SpongeBob=]'s breath)''\\
'''Patrick:''' Uuuuuuglyyyyyyy....\\
** Also used in "Bubble Buddy", in dramatic slow-motion and with added vowel screwing around with.
** And used twice in "Frankendoodle."
** Two of them in "New Digs", one by Spongebob when he is late, one by Squidward when Mr. Krabs evicts [=SpongeBob=] from living at the Krusty Krab.
** In the movie, Plankton lets one loose when SpongeBob began zapping the mind-controlling helmets off of the Bikini Bottom residents using ThePowerOfRock.
** Gary does this in ''Treats!''...with a big long meow as a substitute.
** "Glove World R.I.P":
-->'''Glove World Owner:''' Well, enjoy that hat. Now if you'll folks will excuse me, I have a rickety old theme park to close down.\\
'''[=SpongeBob=]:''' Then the rumors are true, you are gonna close Glove World.\\
'''Glove World Owner:''' Yep.\\
'''SpongeBob & Patrick:''' NOOOOOOOOOOOOOOOOOO!!!!!!!!!!!!!!!\\
'''Glove World Owner:''' I understand your concern, but really...\\
'''[=SpongeBob=] & Patrick:''' NOOOOOOOOOOOOOOOOOO!!!!!!!!!!!!!!!
** From "I'm With Stupid"
-->'''[=SpongeBob=]:''' Look! 2+2-4!
-->'''Marty:''' Wow, son! You taught him math, too!
-->'''[=SpongeBob=]:''' NOOOOOOOOO!!!
-->'''Marty:''' ''And'' you taught him to sing!
* The BigNo is a RunningGag throughout ''WesternAnimation/KingOfTheHill''. Nearly always uttered by Hank, in a semi-nonverbal, almost primal-scream manner. [[SugarWiki/FunnyMoments One of the funniest parts of the show.]]
** There's a pretty epic one in "Luanne's Saga", one of the early episodes. The [[http://static.tvtropes.org/pmwiki/pub/images/hankscream_5013.jpg face he makes]] as he does this has become something of a MemeticMutation.
* ''Sev Trek: Pus in Boots'' (the Australian CGI spoof of ''Series/StarTrekTheNextGeneration''). Captain Pinchhard utters one of these upon hearing that [[CreatorsPet Measly Cruncher]] is going to save the ''Enterforaprize'' for the 47th time. He stops this from happening by vaporizing Measly.
* ''WesternAnimation/TheSimpsons''
** In the episode "[[Recap/TheSimpsonsS10E9MayoredToTheMob Mayored to the Mob]]", bodyguard-in-training Homer practices his [[TakingTheBullet slow-motion dive]]. Making it funnier, the instructor is voiced by Creator/MarkHamill (who starred AsHimself in the same episode), who should know his Nooooos...
--->''(Homer gives a running dive with an unenthusiastic Big No)''\\
'''Instructor:''' Well, your dive wasn't bad, but I just didn't believe your 'NOOOOOO'! You gotta sell it! Remember, your 'NOOOOOO' is what gets you your next job! Now drop and give me twenty.\\
'''Homer:''' NNNNOOOOOOOOOOOO!!!\\
'''Instructor:''' Better.
** Used straight in episode "The City of New York vs. Homer Simpson", when Barney discovers he holds the black pickled egg that means he's today's designated driver.
--->'''Barney:''' ''(after noticing the others have normal eggs and slowly seeing he got the black one)'' NOOOOOO! ''(crushes the egg in his hand and holds his head)''\\
'''Homer:''' ''(coming up to him)'' [[CaptainObvious You got the black one.]]
** Also used straight by Bart in "Black Widower".
** Seymour Skinner does [[RuleOfThree three in a row]] in the episode "Bart's Comet", each with increased intensity; firstly, when he discovers that Bart has managed to discover a comet, something Skinner has dreamed of for years, when he briefly left Bart in charge of his telescope whilst trying to capture a less-than-complementary hot-air-balloon depicting Skinner with a giant rear that Bart earlier released ("Noooo!"). Then, he realises that in his earlier grief he's released the hot-air-balloon, letting it float free to humiliate him further ("''Noooo''!"). Then, a paperboy drops the morning paper in front of him, with a headline reading "[[XtremeKoolLetterz PREZ SEZ]]: SCHOOL IS FOR LOSERS": ("'''''NOOOOOOOOOOOO'''''!").
** Skinner pulls a similiar one season 4's "Duffless".
** Done yet again in "Homer and Lisa Exchange Cross Words". Homer makes Lisa so angry that she tells him that she's going to use Marge's maiden name (Bouvier) instead of Simpson. Homer drops to his knees and screams "'''NOOOOOOOOOOO!'''" Then another girl walks by and asks if he wants to buy some Band Candy. Homer's reply: "'''[[BigYes YEEEEEEEEEEEES!]]'''"
** And then there's the episode where the Flanders's get Bart, Lisa, and Maggie as foster children.
--->'''Ned:''' Do you reject Satan, and all his empty promises?\\
'''Homer:''' '''NOOOOOOOOOOOOOOO!!!!!!!''' ''(skin burns on contact with holy water)''
** "Guess Who's Coming to Criticize Dinner?":
--->'''Lisa:''' Dad, no! [The poisoned eclair] is going to kill you!\\
'''Homer:''' Eh, I've had a good run. ''(prepares to take a bite)''\\
'''Lisa:''' No! ''(thinks)'' It's low-fat!\\
** And parodied yet again in an episode where Grandpa is a [[BlandNameProduct Sprawl-Mart]] greeter, and gets knocked onto an out-of-control shopping buggy.
--->'''Grandpa:''' NOOOOOOOOOOOOOOOOO-- ''(cut to show that he's headed for a stack of lawn gnomes)'' --OOOOOOMES!
** In "Homer's Barbershop Quartet", Chief Wiggum lets out one that gets twisted into a wolf-type howl at the moon.
** In the beginning of the episode "The Bart Wants What It Wants" Marge threw the Olympic Torch to the Olympic Administrators and the helicopter crashed and...
--->'''Administrator 1''': Are you okay?\\
'''Administrator 2''': Yeah, I'm ok...(looks at extinguished Olympic Torch) '''''NNNNNNNNNOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!!!!!!!!!'''''
** In the "138th Episode Spectacular", alternate clips are shown including another ending as to who shot Mr. Burns. When he fingers Smithers as the culprit, Smithers gives a big no then adds, "Wait a minute. Yes."
** PlayedForLaughs in the episode where Marge gets a job at the Power Plant. [[ItMakesSenseInContext Smithers is finished cleaning the unirals]] until...
--->(Homer bursts in)\\
'''Homer:''' Oh, man! I really gotta-- (unzips fly)\\
** Apu does this when TakingTheBullet for James Woods in "Homer and Apu."
** In one episode, Bart learns that his nemesis Sideshow Bob is to be set free from prison leading to Big No. However, he learns this in the middle of class while secretly listening to a radio, prompting a rather bemused response:
-->'''Ms. Krapabbel:''' ... Well. Despite Bart's objections, the people of South Africa can now vote in free democratic elections.
** In "Scuze Me While I Miss the Sky", Lisa, who doesn't want the town's lights on at night, lets one out as Mayor Quimby flips the switch turning on every light in town.
** In "Homer the Vigilante", an angry mob shows up at the Simpsons' door after Homer fails to prevent a notorious cat burglar from stealing a cubic zirconium from the museum, leading to Jimbo [[BrokenPedestal losing respect for him]].
--->'''Jimbo:''' You let me down, man! Now I don't believe in nothin' no more! I'm going to law school!\\
'''Homer:''' NOOOOOOOO!
** In "Lisa's Date With Density", after she helped Nelson escape from the cops, he looks to see Skinner's reaction as he's cleaning his house [[ItMakesSenseInContext after a rancid food attack]]:
--->'''Nelson:''' Wait until he sees what I left in his birdbath.\\
'''Skinner:''' NOOOOOOO!
** "Treehouse of Horror IX" segment "Terror in Tiny Town" has Scratchy, after being transported from the TV, falling for Snowball II. Marge thinks it's sweet, then she tells him that he'll have to be neutered.
--->'''Scratchy:''' ''(while holding his privates)'' NOOOOOOOOOOO!!!
** In "Catch 'Em If You Can", when Bart sabotages Homer and Marge's flight to Atlantic City:
--->'''Bart:''' So, Mom and Dad are going to Atlantic City, but their luggage isn't. And Homer's getting the low-fat meal.\\
''(cuts to the outside of the plane that Homer and Marge are on)''\\
'''Homer:''' NOOOOOOO!
* Many in ''WesternAnimation/{{Futurama}}''. In particular, being a parody of melodramatic acting, Calculon the robotic soap opera star is prone to do this sort of thing a lot.
** "Bend Her" has two in one episode. Calculon is being interviewed on a talk show with Coilette as another guest (who is Bender who changed his robotic sex and invented his own country to win multiple fembot Olympic medals). When asked to set the audience up for the clip they are about to show from a new episode, Calculon responds "No, I think it's self-explanatory." The clip shows Calculon screaming "NNNNOOOOOOOOOOOO!" (while a {{pirate}} [[FunnyBackgroundEvent inexplicably cooks burgers on a barbecue behind him]]). After the clip, Calculon nonchalantly replies, "Funny story: The script called for me to say 'yes' [[ThrowItIn but I gave it a little twist]]." Afterwards, "Coilette" ends up engaged to Calculon but has to call the wedding off before the sex change becomes permanent. After... she fakes her death of at what would have been her wedding to Calculon...
--->'''Dr. Zoidberg:''' ''(in the middle of eating and nonchalantly)'' [[HesDeadJim I'm a doctor. He's dead.]]\\
'''Calculon:''' No! NNNNOOOOOOOOOOOO! N! O! O! O!
** Done ''again'' by Calculon in "That's Lobstertainment!", when Harold Zoid (Zoidberg's over-the-hill celebrity uncle) is trying to make a movie that will guarantee him an Oscar.
** In "Mars University", Gunther the genius monkey decides to get a degree in business with his now only decent intelligence. causing Professor Farnsworth to shout a big no.
** In "Love and Rocket", Bender dumps the crazy Planet Express ship while she, and the main cast inside her, are being chased by a large fleet from Omicron Persei 8. Cue big, shrieking no.
** "When Aliens Attack": Fry gets one off after a ship from Omicron Persei 8 [[SeriousBusiness blows up his sand castle]], immediately after the Omnicronians blew up [[BrickJoke many world monuments gathered in the same part of New New York]].
** And Fry again in ''Into the Wild Green Yonder'' when Bender gives him back his game (he was playing Tetris) and Bender tell him he missed up the "L" formation he was making with the blocks.
** Fry ''again'' in "Fry and the Slurm Factory", when Leela dumps the trough of pure Slurm he'd been eating from into the sewers.
** Fry '''''yet again''''' in the episode "Time Keeps On Slippin'" when his love letter to Leela ends up being destroyed.
** When the [[AttackOfTheFiftyFootWhatever giant Bender]] in "Anthology of Interest 1" finally shuts his eye bay after his untimely death, this is the first thing that comes out of Fry's mouth.
*** Gets a CallBack in "Benderama" when [[{{Gonk}} the "disfigured" giant]], [[spoiler:[[BerserkButton pushed over the edge by having his mother insulted and his therapist putting him on hold]], defeats the giant nano-Bender facsimile and sends him crashing to the ground.]] Guess how Fry responds.
*** The above episode also has one during the beginning in an episode of the ShowWithinAShow "The Scary Door". A scientist builds a robot that does everything for him, including shouting "NOOOO!" when the irony of the KarmicTwistEnding sinks in.
** Bender in the episode "Lethal Inspection" when [[spoiler: he learns he wasn't built with a back-up unit]].
** In "The Duh-Vinci Code" when Fry accidentally destroys The Professor's Beard of Leonardo Da Vinchi only for him to find a hidden scroll in it a second later.
-->'''Professor:''' No! Noooooo! But possibly yes.
** Played with by Bender in "Silence of the Clamps" where, after delivering a BigNo as he [[spoiler:witnesses Calculon getting clamped into a misshapen mess in front of the Robot Mafia]], describes the scene in hilariously full detail in front of the chief of police, complete with imitation of said BigNo. When required to testify before a grand jury, he [[spoiler:repeats his bit-by-bit exposition of the Robot Mafia's act, only to have the BigNo reproduction interrupted by the prosecutor. He asks to let him finish, then lets out a brief "o" representing the end of the BigNo, then says that he is, in fact, finished.]]
* In a ''WesternAnimation/RobotChicken'' sketch involving Franchise/{{Archie|Comics}} and his friends [[Franchise/FinalDestination dealing with deaths plaguing everyone]], Moose lets out a Big Duh when Ms. Grundy is crushed by a car.
** In the Star Wars Robot Chicken Special (3rd one), Vader slips on something, and falls. His hand gets stuck in the toilet, and the toilet flushes. Cue the: "NOOOOOOOOOOOO!"
** In the first season finale parodying ''Series/YouCantDoThatOnTelevision'', the Bloopers host is being held prisoner and lets one out when he's told that the only movie his torturers have is ''Film/ChristmasWithTheKranks''.
* ''WesternAnimation/JackieChanAdventures'':
** Shendu yells this after ending up [[DemonicPossession possessing Valmont]], instead of Jackie, which was what he intended. Almost all of the Demon Sorcerers scream this when they are banished back to the Netherworld through their portals. When they banish the Earth Demon, Jade hangs a lantern on it: "Here comes my favourite part. "NOOO!""
** Uncle is also fond of the Chinese version "Iyaaaa!" -- usually just before smacking Jackie around the head for doing something stupid.
* Parodied throughout the ''WesternAnimation/DrawnTogether'' episode "Terms of Endearment", in which Captain Hero screams "Nnnooooooo!!" at ''least'' 7 or 8 times. At one point (as the camera pulls back... and back... and then back some more, to reveal just how big a problem he's facing) Hero screams the Big No three times ''in a row'', with dramatic pauses in between.
* Often used by ''WesternAnimation/SWATKats'' villains such as Dark Kat and The Pastmaster when the heroes defeat them.
* Done straight on a fairly regular basis in ''ReBoot''. [[spoiler: Bob, being shot into the Web at the end of season two, Enzo, after learning about Bob's fate, Dot in "Game Over" when it appeared that Enzo had been nullified, and said ''twice'' by Megabyte in two episodes toward the end]]. [[http://rebootplotpoints.ytmnd.com/ This YTMND]] does a rather good job of pointing out just how often this trope is played.
** [[LampshadeHanging Lampshaded]] in the musical recap at the end of the third season:
--->'''Singer:''' But Megabyte betrayed Bob and
--->He threw him deep inside the pit.
--->The pit was closed,
--->and Bob was hosed,
--->and all that he could say was-
--->'''Actor Bob:''' [[SubvertedRhymeEveryOccasion Noooo!]]
* The ''WesternAnimation/XMen'' animated series:
** A wonderful Big No can be found in episode 4 of season 2, "One Man's Worth part 1". The first occurrence is when Bishop shouts it as Professor X [[spoiler:is assassinated in the '50s before he can found the X-Men]], followed quickly by an epic Big No from Prof. X when [[spoiler:he vanishes from the present]]. The episode ends with a repeat of the opening scene -- but this time, ''it's in slow motion for the last few lines of dialogue'', including Bishop's Big No. [[http://www.youtube.com/watch?v=-1s3_RH_02M Behold]].
** Also there's a Big No from Apocalypse at the end of the four part "Beyond Good and Evil".
** As well as one from {{Wolverine}} in "Out of the Past: Part 2" when Yuriko was touched by the M'Kraan Spirit Drinker.
** In addition, there's one from Cyclops in "Reunion, Part 1" when Sinister and his gang kidnaps Jean.
* Another Big No scene sadly fails in ''WesternAnimation/XMenEvolution'', when Nightcrawler reacts too slow to stop [[spoiler:an angry Rogue from pushing Mystique's petrified body off a cliff and has to see it shatter into a thousand shards right in front of him]], shortly after finding out that Mystique was his mother. Again, the scene would have been so much better ''without'' the slow-motion yell. When recapping that episode in the introductions to later ones, they put the "NOOOO" back at normal speed. It's much more effective.
* ''Franchise/{{Transformers}}''
** Optimus Primal gets quite a few in ''WesternAnimation/BeastWars'', most notably in the cliffhanger of the first season finale, and when [[spoiler: the ''Axalon'' is destroyed]] in season 3.
*** Inverted with Megatron, as he usually says "Yesssssssssssssss."
*** Last episode of Beast Machines. Megatron's final word? "NOOOOOOOOOOOOO!"
** And in ''Armada,'' Optimus Prime gets one of these when [[spoiler: Smokescreen dies]].
*** [[spoiler: Megatron]] later gets one when [[spoiler: Starscream of all people is killed by Unicron.]]
** Parodied in the "Bee in the City" script reading of ''WesternAnimation/TransformersAnimated'', with David Kaye using his ''Beast Wars'' Megatron voice (to enthusiastic approval from the crowd). Upon his defeat, he vows to return, ending with a {{big|Yes}} "[[VerbalTic YEEESSS!]]"
** In ''Animated,'' the Autobots discuss having to leave, and Bulkhead wonders how Sari (human sidekick) will take it. Bumblebee says she's a big girl and can handle it. Cut to Sari screaming '''NOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!''' at the top of her lungs.
** Not to mention the episode "Nature Calling" when Prowl smashes Bumblebee's only battery, rendering him unable to see movies in the middle of nature. His "NOOOO!" was truly epic.
* In ''WesternAnimation/TheSpectacularSpiderMan'', the Sandman gets one of these after seeing the results of his TransformationTrauma into a living [[BlobMonster sand being]].
* Littlefoot gets one of these in ''[[WesternAnimation/TheLandBeforeTime The Land Before Time IX]]: Journey to Big Water'', as he watches Mo lead the swimming sharptooth away.
* Used in ''WesternAnimation/AmericanDad'' by either Stan, Francine or Hayley when [[spoiler: they believe Steve and Roger were killed by a lightning-struck treehouse]]. Rather effective due to how strangled and broken it sounded. Steve also gets a particularly good one.
** Stan has had a couple of these.
* ''WesternAnimation/AvatarTheLastAirbender''
** There's a slow-motion one from [[spoiler:Zuko]] in the GrandFinale when [[spoiler:TakingTheBullet for Katara]]. Cue "must run in the family" jokes...
** It really does; Iroh has a (much less dramatic) one when he sees [[BountyHunter June]] get hit with her Shirshu's paralyzing tongue.
** Sokka has one in "The Desert", right before Aang creates a giant mushroom cloud.
** Sokka also lets out one in "The Cave of Two Lovers", when he finds he's trapped in a cave with the [[NewAgeRetroHippie nomads]].
** Parodied in the WhoWouldWantToWatchUs? episode when Actress Aang lets out a nice 9 second "NOOOOOOOOOOOOOOOO!" [[spoiler:as she "dies"]].
** Katara has one in the Puppetmaster when [[spoiler: Hama nearly forces Sokka and Aang to kill each other using Bloodbending]].
* ''WesternAnimation/TheLegendOfKorra'' (SequelSeries of ''WesternAnimation/AvatarTheLastAirbender''):
** Unavaatu pulls out one while [[spoiler:being spirit bent and destroyed by Korra]].
** Korra also has one of these in the Book 1 episode "Endgame" when she sees that [[spoiler:Mako is about to have his firebending removed by Amon]]. Even though she has [[spoiler:already had her own powers removed, her desperation to save Mako leads to a CrowningMomentOfAwesome when she finally unlocks her airbending]].
* ''WesternAnimation/DannyPhantom''
** When Danny's family and friends (and teacher) are about to be blown up to their doom in an [[BadFuture alternate timeline]], Danny cries one out, complete with slow motion movement.
** Vlad later gets one in another episode when his perfect clone is destroyed by Danny and his OppositeSexClone. For just one word, it's brilliantly acted.
* In one episode of the 2003 ''WesternAnimation/{{Teenage Mutant Ninja Turtles|2003}}'' series, Baxter Stockman lets out a big "WHY?" after Bishop brought him back from the brink of death (as a result of the events in "Insane in the Membrane"), [[UnwantedRevival which robbed him of finally]] [[NeverSayDie resting in peace]] after everything he [[ButtMonkey lived through in the series]]. It...comes across a bit [[{{Narm}} narm-y]].
* ''WesternAnimation/KimPossible''
** In "Stop Team Go", after using hundreds of mooks to destroy the gurken jar, [[HarmlessVillain Dr. Drakken]] puts the jar under a big laser beam, and he fires it. The result was a big crater on the floor, and the jar was unharmed. Cue Big No.
** In another episode, upon learning that her very annoying younger twins have been skipped ahead and are now in the same high-school as her, Kim lets out a big no powerful enough to knock letters of the school sign out front, while she's indoors.
** Ron has a couple throughout the series (including a truly epic one in "A Very Possible Christmas" (Season 2, episode 16).
* Being a ''Franchise/StarWars'' animated series, ''WesternAnimation/StarWarsTheCloneWars'' has instances of the use of the BigNo trope:
** In Brain Invaders, Barriss Offee screams "NO!!!" [[spoiler: as she gets infected and possessed by a Geonosian Brain Worm offscreen]].
** Anakin pulls off this trope twice in the Mortis Trilogy. First, [[spoiler: when the Son of Mortis kills Ahsoka]], and second when [[spoiler: when the Son shows him what he will become in RevengeOfTheSith]].
** ARC Trooper CT 27-5555 "Fives" screams one when [[spoiler: Hardcase]] tells him that he's going to sacrifice himself to destroy a Separatist Supply Ship after the shields to the reactor core were raised.
** Obi-Wan does it a couple of times: first, a quick one when Zygerrian Prison Warden Keeper Arguss [[spoiler: activates the control panels to the slaves' quarters to drop them all into the pit of doom below.]] The second time is right before [[spoiler: Savage Opress gives Adi Galia the finishing stab with his lightsaber after impaling her with his horns]].
* ''WesternAnimation/ThePowerpuffGirls''
** [[http://www.youtube.com/watch?v=heNRLR2d6jo&feature=related "Parent teacher conference?! NOOOOOOOOOOOOOOOOO!!!"]]
** From "Mo'Linguish":
--->'''Judge:''' You, Mojo Jojo, are hereby sentenced to teaching a knowledge extension course at Townsville Community College!
--->'''Mojo Jojo:''' NOOOOOOOOOOOOOOOOOOOOO! By which I mean I cannot do it.
** "Fallen Arches": [[OldSuperhero Captain Righteous]] and Lefty confront the Ministry of Pain. The Captain lets out a big no as he runs towards Lefty who just hurt his hip. However, he hasn't reached his sidekick yet so [[Funny/ThePowerpuffGirls he shouts it again]].
** "Get Back Mojo": Mojo Jojo lets out a frustrated "NOOOOOO!" at the end of the episode when the Narrator points out that his trip through time [[StableTimeLoop accidentally inspired the creation of the Powerpuff Girls]].
** The comic book story "Steal A Meal" (Cartoon Network Block Party #26) has the girls doing a tearful BigNo upon learning that after all the breakfast cereal in town had been stolen the only thing left was oatmeal.
** "Mime For A Change": Bubbles performs a great BigNo.
** For the good example in "Knock it Off": [[KnightofCerebus Dick Hardly]] does this when his own creations start killing him.
* Parodied in the ''WesternAnimation/SpaceGhostCoastToCoast'' episode "Boo Boo Kitty", when Space Ghost's pet cat, actually a large balloon, slowly drifts towards a large safety pin. He screams NOOOOOOOOOOOOOO!, runs out of breath, inhales, and starts again. After the balloon deflates, he promptly ignores the others when they suggest he could have easily saved Boo Boo Kitty from its untimely death.
* Played for laughs in ''WesternAnimation/{{Metalocalypse}}'', where Big Nos are Nathan's catchphrase, and he lets them out quite regularly. They are usually accompanied by the camera flying down Nathan's throat. One example comes when Nathan lets out a NOOOOOOOOOO! upon Nick Ibsen revealing Dethklok's parents.
-->'''Nick Ibsen:''' You are all going to be reunited with your families.\\
''(a panel opens up, revealing the families of each of the five band members)''\\
'''Nathan Explosion:''' ''NOOOOOOOOOOOOOOOOOOOOO!!!'' You're going to pay for this!\\
'''Nick Ibsen:''' Pay for what? Journalistic integrity?
** The bit on flying down the throat comes from the episode "Murdering Outside the Box". When Nathan learns that someone is embezzling from the band, he suddenly goes into the Big No sequence, and from the "down the throat" bit the show goes into the opening.
** It also happens when Nathan, after having death-growled the entire works of Shakespeare to sell as an audiobook, learns that an extremely-inebriated Pickles "like...TOTALLY forgot to hit record, man." The strain of recording the growled version of "Hamlet" having taken its toll on Nathan's larynx, the BigNo was rasped rather than screamed.
** It's hard to identify due to the polyphony of the crowd chanting, Nathan screaming, and Skwisgaar panicking, but this is what Pickles is yelling at the end of the Depths Of Humanity in ''[[WesternAnimation/MetalocalypseTheDoomstarRequiem The Doomstar Requiem]]''.
* The ''WesternAnimation/BuzzLightyearOfStarCommand'' episode "Plasma Monster" has Booster react this way when Plasma Boy's girlfriend tells him they can still be JustFriends.
* ''Series/AceLightning'': The characters in this mixed media show are based on superhero stereotypes, so this was going to show up sooner or later -- except that it's ''not'' one of the CGI characters that do it -- it's a ''human'', in the last episode, when he thinks Ace has been shot.
* In ''WesternAnimation/StreetFighter'', Guile gives one when Cammy defected to Shadaloo and gives Bison a '''big kiss'''. [[http://www.youtube.com/watch?v=0_5LnKVfS5M Note]] the SoundtrackDissonance.
** Also used in some episodes, such as the one episode when Vega yells this after Blanka attacked Vega's face and after Vega looks at his reflection in the water.
** In another episode, Fei Long yells this twice.
** During "The Strongest Woman in the World," a young Chun Li yells this in a flashback as she witnesses M. Bison killing Chun Li's father.
** The final one is delivered by Cammy in the final episode when she realizes that everything that M. Bison said was lies.
* In the ''WesternAnimation/{{Freakazoid}}'' episode "House of Freakazoid", Freakazoid is pressured to pursue a villainess through a sewer, but repeatedly refuses because he hates the smell of poo gas.
-->'''Freakazoid:''' NO! A big N, followed by a little O: no!
* Parodied in ''WesternAnimation/TheFairlyOddparents'' a few times:
** Played straight in the ''Fairly Oddparents: Shadow Showdown'' video game; Timmy shouts it after his TV breaks in the introduction, and again upon seeing he's missed the ''Crash Nebula'' season finale at the end.
** And parodied in "Pixies Inc", where, after being foiled, the Pixies let out a big no--[[DullSurprise with no emotion]].
** Cosmo and Jorgen in "Cosmo Rules", when they learned they're cousins. Their BigNo lasted nearly 24 hours as the scene showing the sun setting and rising again suggested and then Jorgen fainted and Cosmo added ..."WAY!" Cosmo was enjoying the idea and even planned cousingly activities for him and Jorgen. Albeit not all of them were fit for ''male'' cousins.
** Crocker in "Formula for Disaster" at the end when he is trapped on a bus headed to Cincinnati he yells "NOOOOO! Not Cincinnati!" A sign saying Welcome to Cincinatti! Crocker free since 1983 may have something to do with it.
** Chester also did this in "The Boy Who Would Be Queen:" when A.J. fell in love with Timmy's girl form "Timantha."
* Cow of ''WesternAnimation/CowAndChicken'' did it in an episode while jumping to catch a coin. In slo-mo. [[MundaneMadeAwesome With the entire world slowed down as well]].
* In the last episode of the third season of ''WesternAnimation/TheVentureBrothers'', Henchman 21 indulges in a Big No when the Monarchmobile explodes [[spoiler: with Henchman 24 trapped inside]], but is interrupted when [[spoiler: 24's severed head]] hits him in the chest.
* In ''Disney/BrotherBear'', Kenai lets out the distant, bird-disturbing version of a Big No when he discovers he's been transformed into a bear.
* In ''WesternAnimation/BatmanBeyond'', Derek Powers accidentally inhales a big gulp of his own nerve gas, and in the same second it takes him to realize what has happened to him, makes an appropriately horrified scream to this effect.
** [[MakeMeWannaShout Shriek]] also does this once he realizes [[HoistByHisOwnPetard his own sound amplification device has rendered him deaf.]]
** Terry does this when Inque knocks Bruce Wayne across the room. A few minutes later, Inque does this when Terry shatters the glass ceiling and lets the rain in, which dissolves her.
* An episode of ''WesternAnimation/FamilyGuy'' has Peter be ''really'' upset over the loss of his pet parrot.
** Peter again in "[=McStroke=]" when his mustache burns off.
* The ''WesternAnimation/DonkeyKongCountry'' episode "Buried Treasure" contains this exchange when Klump's video conference interrupts K. Rool's computer game:
-->'''Klump:''' General Klump reporting, King K. Rool sir! Y'read me, your high 'n mightyness?... Looking a little pale there, chief!\\
'''K. Rool:''' '''''NNNNNNNNNNOOOOOOOOOO!!!!!!!!!!!!!!!!!!!''''' ''(smashes the computer)''\\
'''Klump:''' ''(calling on the phone)'' Chief? You there, chief? Seems we had some sort of interference.
* In an episode of ''WesternAnimation/TheMarvelousMisadventuresOfFlapjack'', The Real Adventurer has the reaction to Bubbie telling him K'Nuckles is not a pregnant woman.
* ''WesternAnimation/InvaderZim'' just loves to scream. The Big No is his favourite.
** From the same show we also have [[http://www.youtube.com/watch?v=-oLmHpzBqdk the most epic Big No]] of all time as delivered by a random guy at a park bench who's soda can is ''[[ItMakesSenseInContext being crushed by Mars]]''.
** And then there's two Big No's in the episode "Zim Eats Waffles".
* ''WesternAnimation/SouthPark'':
** In the episode "Cat Orgy", when Skyler discovers his trashed guitar.
** The episode "Jewbilie" does it ''four times in a row'': First Kyle when Kenny makes a HeroicSacrifice to stop Haman, then Haman when Moses escapes the conch shell, then anti-semetic Rabbi Garth screams it twice when he realizes his plans have been foiled.
** "Probably": God gives Saddam Hussein [[FateWorseThanDeath an even worse punishment than sending him to Hell: sending him to Heaven]].
--->'''Mormon:''' We were just about to do a play about how much stealing hurts you deep inside. Come join us. ''(the Mormons lift Saddam over their heads and carry him off)''\\
'''Saddam:''' No! NOOOOOO!
** "Something You Can Do With Your Finger" parodies the ''Film/StarTrekFirstContact'' example above, when Randy prevents Stan from being in his boy band. Incidentally, they actually use the sound clip of Picard's breakdown from the movie for that scene.
--->'''Stan:''' Why can't I be in a boy band?\\
'''Randy:''' ''(firmly)'' BecauseISaidSo.\\
'''Stan:''' Cartman said we're going to perform at the mall at three o'clock! My friends are gonna be pissed off at me!\\
'''Randy:''' ''(still pretty controlled)'' Let them be pissed off.\\
'''Stan:''' Dad, I don't understand. Just let me do this one thing and then I'll...\\
'''Randy:''' ''(inexplicably losing it)'' ''Noooo''! ''NOOOOOOO''! ''(smashes a glass dresser with his head)''
** In "The Last of the Meheecans", Randy does it again when he's confronted with what happens when Mexican immigrants performing menial labor all decide to move back to their birth country in a fit of nostalgia, using the same Darth Vader soundclip from ''Film/RevengeOfTheSith''.
** In "Marjorine" Linda Stotch lets out several blood-curling screams of "NO" when she thinks her son Butters has committed suicide by jumping off a building. She does it again at his funeral while throwing herself on his casket.
* ''WesternAnimation/RockosModernLife'': Rocko and Heffer are on a bus tour of Paris with a bus driver who is determined to keep his passengers on the bus ''[[AxCrazy at all costs]]'', so much so that he chases them around the city after they escape. By the time the two board a plane home, the bus driver [[http://www.youtube.com/watch?v=1j7bGDv9hY0 delivers an epic BIG NO]] as the two fly off into the sunset.
* ''WesternAnimation/SpiderManTheAnimatedSeries'':
** Blade lets out a particularly {{narm}}tastic one of these in the season four episode, ''The Vampire Queen''.
** And Spider-Man gives a particularly haunting one upon [[spoiler:the death of the Mary Jane Clone]].
* ''WesternAnimation/TotalDramaIsland'':
** Owen says a big no when a box of chocolates gets knocked into the water.
** Also, Alejandro in the finale of the 3rd season gives out a Big No after he is [[spoiler:put in a robot suit because he was burned by lava and trampled by most of the contestants]]. Al lets it out when he asks Chris if the prize money is safe, only to find out [[spoiler:it was burned in the volcano]]. It's hilarious because it parodies the cheesiest moments in Star Wars history.
* ''WesternAnimation/PhineasAndFerb'': Yes, Yes it does have it too, for example:
** Parodied in "Lawn Gnome Beach Party of Terror" the radio DJ at first lets out what appears to be a big no... but he's actually screaming "Gnomes"!
** In "Gaming the System", Candace does this in response to her brothers' DisneyDeath. A few minutes later, [[ShipTease Phineas yells one when the game's final boss goes after Isabella]].
** In "The Chronicles of Meap", [[EgomaniacHunter Mitch]] apparently lets off a Big No when Meap confiscates his [[TranslatorMicrobes Universal Mustache Translator]], but it comes out as a Big [[PokemonSpeak Meap]].
** Candace lets out another Big No in "Invasion of the Ferb Snatchers", on realizing she's missed yet another chance to bust her brothers.
** And Baljeet gives a rare stupidity-induced one in "When Worlds Collide" when trying to explain to Buford what it means when worlds collide, and Buford says "It's like when you've got two sets of keys, and you leave one in the car."
* Parodied in the MusicalEpisode of ''WesternAnimation/{{Daria}}''. Daria gives a Big No as Kevin accidentally locks the four of them out on the roof.
* In one episode of ''WesternAnimation/AdventuresOfSonicTheHedgehog'', Robotnik lets out a Big No which is used extremely often in WebAnimation/YouTubePoop. This video is also an OverlyLongGag.
* ''Dante's Inferno'': An Animated Epic - Dante lets out a pained and enraged (and kinda narmy) big no as he beats on the Gates of Hell that separate him and his wife's soul.
* This has happened more than once in ''WesternAnimation/WinxClub''.
* Mung Daal lets one out during an episode of ''WesternAnimation/{{Chowder}}''.
* ''WesternAnimation/BatmanTheAnimatedSeries''
** Episodes "Pretty Poison" and "Eternal Youth", each near the end, have the villainess Poison Ivy saying this trope.
** Subverted in "Two-Face".
--->"[[https://youtu.be/N2XFm7iX-ds Harvey! Noooo....]]"
** The episode "His Silicon Soul", near the end, has the cyborg Batman saying it after [[spoiler: the real Batman seemingly fell to his death before Cyborg Batman committed suicide. Then it's revealed that Alfred sees a light below which is Batman himself, who survived]].
** Used in the episode "Mad Love" by Harleen Quinzel after SelfDemonstrating/TheJoker gets captured again and taken away from her which helps to show us her SanitySlippage.
* A few ''WesternAnimation/LooneyTunes''/''Merrie Melodies'' cartoons have this:
** The Creator/ChuckJones Warner Brothers cartoon "WesternAnimation/DuckAmuck":
--->'''WesternAnimation/{{Daffy|Duck}}:''' All right. Let's get this picture started.\\
''(IrisOut and THE END appears)''\\
'''Daffy:''' Nooooo! Nooooo!
** The Creator/FrizFreleng cartoon "Bucaneer Bunny" has Yosemite Sam (a.k.a. Pirate Sam) say a couple of Big No's when Bugs attempts to throw a matchstick into the gunpowder stores of his pirate ship.
** Also one near the end of the [=McKimson=] short "Sleepy Time Possum" in which Paw Possum, disguised as a dog, gets catapulted by his son.
* The ''WesternAnimation/{{Bonkers}}'' episode "In the Bag" has Lucky Piquel say this trope when both Bonkers and Lucky are chasing the bag in his police car only to find that the brakes are missing.
* ''WesternAnimation/JimmyTwoShoes'': [[TheHedonist Beezy]] lets out one as [[EnfanteTerrible Heloise]] beats him in a staring eyes competition.
* ''WesternAnimation/EekTheCat'' lets one out in the episode "Cape Fur", when he's being dragged off by [=JB=] for punishment after he hears about the KillerRabbit on the news.
* ''WesternAnimation/AKindOfMagic'': Cindy lets out a Big No in the episode "Meet the Parents" when her LoveInterest Leo [[{{Fainting}} faints]] from sniffing her dirty socks.
* ''WebAnimation/HappyTreeFriends'', while not exactly a "No!", has the very drawn-out "Yarr!" in the episode ''Sea What I Found''. Given the [[SpeakingSimlish circumstances]], it's obvious that he means no.
* ''Series/TheSuperMarioBrosSuperShow'': Koopa has one in "Elvin Lives" when he realizes he's driving his car towards a cliff.
** This exchange from "Princess, I Shrunk the Marios":
-->'''Koopa:''' Know what I'm gonna do with you buttinskis?\\
'''Mario:''' Say you're sorry and let us go?\\
'''Koopa:''' NO!! I'm gonna lock you up for two hundred years!
** Koopa lets out yet another in the ''WesternAnimation/SuperMarioWorld'' episode "Rock TV" when he's about to get crushed by his giant television.
* ''WesternAnimation/SpaceGoofs'':
** Etno lets one out in the episode "Once Upon a Time" when Candy presses the button that launches his spaceship.
** Etno again in the episode "One Minor Technicality" when a boy gets his spaceship box.
* In ''WesternAnimation/TheAdventuresOfJimmyNeutronBoyGenius'' Jimmy gets one when he travels to the future and he discovers that his future self married Cindy. And when it comes back from commercial, we discover that he screamed for four minutes.
-->'''Carl:''' "Wow, Jimmy, you just screamed for [[LeaningOnTheFourthWall 4 minutes]]."
** Sheen lets out one in the interstitial shorts after his Ultra Lord action figure gets stuck in a tree. Also lets one out in "Ultra Sheen" when he witnesses Ultra Lord's defeat at the hands of Robo-Fiend inside the video game.
** Sheen lets out another in "Journey to the Center of Carl" after Jimmy tells him to pretend that Ultra Lord got canceled.
** Hugh does this in "Attack of the Twonkies" when Judy takes Flippy from him and throws him to Jimmy so he can power up his rocket and send the giant Twonkie back into space.
* A ''WesternAnimation/TomAndJerry'' [[http://en.wikipedia.org/wiki/That%27s_My_Mommy short]] revolved around a duckling imprinting on Tom and Tom taking advantage of this to attempt to cook the duckling. Near the end, the duckling realizes that Tom intends to eat him and prepares to [[HeroicSacrifice take a long walk off a soup spoon]] into a bubbling pot, [[http://www.youtube.com/watch?v=xdpNZmdoGzM "If my nice mommy wants a duck dinner..."]] Tom utters a Big No as he catches the duckling. A "No!" made all the more impressive by the fact that Tom and Jerry usually don't have speaking voices....
** This could easily double as a SugarWiki/{{Heartwarming Moment|s}}.
* Happens in an episode of ''WesternAnimation/NightmareNed'' by the Tooth Fairy when Ned [[ChessWithDeath wins a game of checkers against a giant tooth for ownership of his teeth]].
* ''WesternAnimation/SabrinaTheAnimatedSeries'': Gem lets one out in the intro after getting her clothes magically changed by Sabrina's broom zooming past her.
* Little Dog in the ''WesternAnimation/TwoStupidDogs'' episode "Cat!" after Big Dog goes back to sleep without scaring the cat.
* This is WesternAnimation/JohnnyBravo's reaction to finding out the Easter Bunny isn't real.
* ''WesternAnimation/{{Wakfu}}''
** Adamaï lets out a Big No (with SkywardScream) at the end of episode 16, after Grougaloragran teleports the heroes away to fight Nox alone.
** Nox, as part of his VillainousBreakdown in the season 1 finale, when [[spoiler:he realizes that his attempted TimeTravel only brought him back ''twenty frigging minutes'' in the past]].
* ''WesternAnimation/DextersLaboratory'':
** Happens quite a lot. One time in particular, when Mandark forces Dexter to shut down his laboratory.
** In "Jeepers Creepers, Where is Peepers," the transforming Peepers yells this when he sees Dexter and Koosalagoopagoop being attacked by Hokochu before breaking out of his container.
** The Ice Cream Man, after Dexter asks if he's got change for a hundred. This is after the Ice Cream Man explains that he hates Dexter for paying in pennies.
* The Death Kwon Do instructor in ''WesternAnimation/RegularShow'' gets three rather nice ones. First when he discovers Rigby stole the instructions to learn the Death Punch of Death, only to be interrupted by a student explaining the toilet was 'Death Kwon clogged', leading to another BigNo. Then finally, when he discovers that Mordecai stole the Death Jump and Death Dump instructions, he lets out another BigNo.
** Benson seems to be fond of this trope. He had done this in a flashback in Stick Hockey, after being fired in his thoughts in Think Positive, Prankless when he sees Mordecai and Rigby get shot with tomatoes, when Mordecai loses on purpose in Dodge This, a flashback in White Elephant Gift Exchange, and Benson's Suit when his suit was stained.
* Scaredy Bat lets one out in an episode of ''WesternAnimation/RubyGloom''.
* ''WesternAnimation/MyLittlePonyFriendshipIsMagic'' likes this trope, with many instances about equally split between straight uses (by every BigBad in his/her moment of defeat, except the one who [[Recap/MyLittlePonyFriendshipIsMagicS3E2TheCrystalEmpirePart2 is actually destroyed]]), characters who seem to be deliberately hamming it up, and comedic uses with no in-universe hamming.
** Spike does this ''twice'' in "A Dog and Pony Show", both times coupled with SkywardScream: the first when Rarity is kidnapped, and another when he realizes he and the other ponies have gotten themselves lost in the Diamond Dogs' mine.
** In "Stare Master", Fluttershy's reaction to Applebloom suggesting the Cutie Mark Crusaders try to get coal-mining cutie marks is a very emphatic [[ShrinkingViolet (especially for her)]] "NO!"
** Scootaloo does this in "Cutie Mark Chronicles", when Fluttershy suggests singing another (cutesy) song.
** In "Sweet and Elite", Rarity gives a [[ThisIsGonnaSuck rather mournful]] one when her friends decide to crash the Canterlot Garden Party.
** The Cutie Mark Crusaders do this at the end of "Hearts and Hooves Day" when Big Macintosh and Cheerilee make the Crusaders think the two of them are still under the influence of the love poison.
** Several in "Games Ponies Play": first, we see Rainbow Dash give one in a flashback after she learns that Cloudsdale won't be getting the Equestria Games this time. A minute later Pinkie does one that seems to come out of nowhere until she explains that she was just answering Twilight's question ("Are we going to let Cadance down?") Later, when a panicked Rainbow Dash asks, [[MistakenForSpecialGuest "We got the wrong pony?!"]], Pinkie screams, "NOOOOOOOOOOOOOOOOOOOOO!" -- before correcting herself to a BigYes in exactly the same tone.
* In ''WesternAnimation/VeggieTales'', Larry the Cucumber did this when Archibald inserted "His Cheeseburger" in the jukebox. [[http://www.youtube.com/watch?v=SeEihNdXxdc "NOOOOOOOOOOOOOOO..."]]
** By Haman (Mr. Lunt) in ''Esther... The Girl Who Became Queen'' when he finds out he's getting banished to the Island of Perpetual Tickling.
** Two by Don Quixote (Archibald Asparagus) in ''Asparagus of La Mancha'', one when he wakes up from his first bad dream, one when Poncho (Mr. Lunt) weans him off the salsa, due to finding out that Don's addiction to his super-spicy salsa is causing the bad dreams.
** By The Bad Apple in ''Larry-Boy and the Bad Apple'', when her webs dissovles by using water.
** By Archibald Asparagus in the silly song ''The Biscuit of Zazzamarandabo'', when everyone needs to take a potty break.
** Also by Larry in the silly song ''Sippy Cup'' combined with {{Eat The Camera}} when Jimmy Gourd explains why Larry needs a sippy cup.
* Johnny delivers one in ''WesternAnimation/JohnnyTest.'' And then [[SugarWiki/MomentOfAwesome the ground shakes]].
* Richard in ''WesternAnimation/TheAmazingWorldOfGumball'', when told he has to get a job.
** As a child he did one that ''[[UpToEleven lasted for 15 years]]''.
* Princess Sally yells it in Sonic's bad dream when she is roboticized in ''WesternAnimation/SonicSatAM'', episode "Sonic's Nightmare".
* Uttered by Kabuto at the end of ''[[WesternAnimation/PixarShorts Tokyo Mater]]'' as a result of him losing to [[WesternAnimation/{{Cars}} Mater]] in a drift race and is about to have all of his modifications pulled off his body by all of the other cars in Tokyo, who then laugh at him when Kabuto is finally naked.
* ''WesternAnimation/{{Recess}}'' uses this a number of times, a few being "No...no...NOOOOOOO!!!!!":
** "The Break In": Towards the end, by T.J. when he just broke himself out of detention and onto the playground, just as recess ended for the day.
** "The New Kid": By Gus when his name is taken away and must be refered to as "New Kid"
** "The Experiment": By T.J. and Spinelli when they find out they have to kiss each other to see what the effects were on the opposite sex.
** "The Legend of the Big Kid": By Vince when he and the gang are looking for T.J.
** "Mama's Girl": By Spinelli when no one will stop taunting her after she accidentaly called Miss Grotke "mama".
** Mikey, in various episodes.
* In ''WesternAnimation/{{Thundercats 2011}}'' Lion-O performs one that overlaps with a SlowNo and a FutileHandReach upon seeing his father King Claudus stabbed InTheBack.
* Franchise/GreenLantern pulls off a gloriously [[LargeHam hammy]] one when he's trapped inside a yellow smiley face time bomb in ''WebAnimation/DCSuperFriends''.
* ''WesternAnimation/MyLifeAsATeenageRobot'': Vexus at the end of "Escape From Cluster Prime", when Vega and her friends give Cluster Prime back their golden chips. Also, used in "Puppet Bride" by Mrs. Wakeman (along with a flying tackle) during a wedding between Little Acorn and Jenny.
* Happens quite a lot on ''WesternAnimation/AdventureTime''. In "Wizard Battle", Finn has to win a contest in order to win a kiss from his love interest, Princess Bubblegum. When one of the other contestants almost wins, he lets out a Big No that reaches the level of a SonicScream.
** Finn also lets one out in "Mystery Train" when he sees that [[spoiler:Jake has [[DisneyDeath seemingly]] been killed by the Conductor]].
** Most notable example is The Lich in the season 4 finale. Finn is about to destroy the Enchiridion, so he lets out a BigNo. But then, as it turns out, [[BatmanGambit he was counting on Finn destroying the Enchiridion after he did so]], which is what allowed the Lich to access the Time Room and attempt to wipe out all life... [[NiceJobBreakingItHero oops]].
* WesternAnimation/AquaTeenHungerForce: Meatwad gives one when he finds out Shake ate Inside-Out Boy.
* ''WesternAnimation/TransformersPrime'':
** Knock Out does one when Starscream punishes him (by scratching his precious finish) in "Speed Metal".
** Miko does one when Bulkhead almost gets beheaded in "I.M.I.".
** Arcee yells out a "No!" when [[spoiler: a replication of Unicorn was about to kill Optimus.]]
** Megatron gets a magnificent one during "Orion Pax Part 3" when [[spoiler: Jack restores Optimus's memories and the Autobots escape.]]
** In the GrandFinale, Optimus' reaction to [[spoiler: Bumblebee's apparent death]] is half this, half an inarticulate scream of rage.
* ''WesternAnimation/TheRenAndStimpyShow'': In "Sven Hoek" Ren lets out a BigNo after he sees that Sven and Stimpy ruined his collection of rare incurable diseases.
* Used in ''WesternAnimation/TheWeekenders'', during the episode "Cry", when the gang is making fun of Tiro shedding a tear over ''Romeo and Juliet'' while hanging out at the [[LocalHangOut pizza place]]:
-->'''Carver:''' How can anyone get upset over something as dumb as that?\\
'''Lor:''' [[ItMakesSenseInContext Pizza's jammed.]]\\
'''Carver:''' [[HypocriticalHumor NOOOOO!!!]]
* ''WesternAnimation/SamuraiJack'' episode 23 "Jack versus Demongo, the Soul Collector". Demongo uses one after Jack defeats him.
** And in Season 5, Jack himself lets out one when he believed the blue-furred children from the factory are dead.
* ''WesternAnimation/TheAvengersEarthsMightiestHeroes'' has Kang the Conqueror say this as he gets banished from the Avengers' time period.
* ''WesternAnimation/DaveTheBarbarian'' is prone to parodying this. It has often been used over decidedly minor things or for humour's sake. Such as this:
-->'''Dave:''' Is my nose clean now?\\
'''Dave:''' [[LampshadeHanging I was only asking]]...
* In the first episode of The Hub's ''WesternAnimation/{{Pound Puppies|2010}}'', adoptee-of-the-week Yipper lets out a cross between this and a howl of despair when it looks like he's missed his chance to get adopted by his "perfect person".
* Julie does this in the ''{{WesternAnimation/Motorcity}}'' finale when [[spoiler:she thinks Mike was killed in the self-destruction of his car]].
* In an episode of ''WesternAnimation/CampLazlo'', Scoutmaster Lumpus does it when Slinkman says that he can earn his "Handy Helper Badge" if he can...gasp!...'''help someone!'''
** Edward does this when he finds out that his favorite doll Veronica has gone missing, which echoes through the camp. Lazlo, upon hearing it, responds with ''"[[BigYes Yeeeesssssss!!]]"'' in response [[CloudCuckooLander despite not knowing the context.]]
* In the first episode of ''WesternAnimation/{{SheZow}}'', Guy has this reaction once he learns that his aunt's magic ring won't come off his finger and that he would be forced to carry the duty of [=SheZow=] for the rest of his natural life. This inadvertently triggers his [[MakeMeWannaShout sonic scream power]] and he destroys the mirror guarding the entrance to his aunt's secret lair.
* In ''WesternAnimation/TheGrimAdventuresOfBillyAndMandy'' episode "Billy & Mandy Moon the Moon", this is Mandy's reaction to [[MakesJustAsMuchSenseInContext being turned into a milk-themed superhero]].
* One episode of ''WesternAnimation/CelebrityDeathmatch'' has Elizabeth Hurley and Anna Kournikova fighting atop a cage filled with horny teenage boys. Hurley manages to pound Kournikova into the cage, followed by a series of limbs and carnage flying from the top. Hurley assumes that the boys have ripped her opponent to pieces, until a pair of hands grab at her feet from beneath.
--> '''Hurley:''' "Mr. Lane, get these boys off me!"
--> '''Kournikova:''' "Guess again, Hurley!"
--> '''Johnny Gomez:''' "[[NotQuiteDead Anna Kournikova is alive!]]"
--> '''Nick Diamond:''' "[[TheReveal She must have ripped those boys to pieces!]]"
--> '''Hurley:''' "NOOOO!"
** Johnny Gomez pulls one of these after his co-host, Nick Diamond, is thrown from the announcer's booth after it is damaged by an air-born Roger Ebert.
** Nick also does one of these during the Congressional Hearings episode, after Senator Ted Kennedy sentences him (and Johnny) to no more than 10 hours of community service.
* ''WesternAnimation/BeavisAndButthead'' - Beavis often screams out a lengthy "AAAAAAAAAH NOOOOOOO!!!" if he'd distressed, often by a video that sucks.
* The first episode of ''WesternAnimation/StevenUniverse'' opens with the title character letting out one of these when he finds [[FauxHorrific his favorite snack, Cookie Cats, has been discontinued]].
** Amethyst gets in on the act in "Monster Buddies":
--->"Noooooooo! [[GratuitousSpanish Mi]] [[LostFoodGrievance torta]]!"
* ''WesternAnimation/BobsBurgers'' - a very deadpan version, when Tina becomes complicit in insurance fraud, and imagines going to Jail-Hell.
-->'''Tina''': What's for lunch?
-->'''Jail-Hell Lunch Lady''': Your lies!
-->'''Tina''': Nooooo! That's what I had yesterday.
* In ''WesternAnimation/RickAndMorty'', Rick lets out a couple after a group of scamming aliens fully reveal the nature of their scheme to him.
** He lets out another in the season 2 finale when [[spoiler: Birdperson, his best friend, is killed]].
* In the ''[[WesternAnimation/KaBlam Life With Loopy]]'' short "Kung Fu Loopy", Larry lets one out after his mom suggests that he should take Loopy to "Kung-Fu Chickens On Ice" with him. His narration describes this as his "[[UnreliableNarrator convincing argument]]"
* ''WesternAnimation/ThreeTwoOnePenguins''
** In ''Comedy of Errors'', Michelle does this when [[spoiler: Jason sings the song she sings when she's scared at the Comet Lounge.]]
** In ''Wise Guys'', Jason does this twice, and both times coupled with a SkywardScream: the first when he discovers that the batteries are not included with the time machine, and another when he is told [[spoiler: that he and his sister are stuck in the future forever]].
** In ''Oh, Mercy!'', Zidgel, Jason and the Gator King yell this in slo-mo when the emerald falls out of its case and smashes into many pieces.
* ''WesternAnimation/BumpInTheNight''
** In the episode "Night of the Living Bread", the Bread hollers "NO!" right before Mr. Bumpy knocks him down by flinging peanut butter at him.
** The Cute Dolls scream "No!" at the end of "Party Poopers" after Mr. Bumpy promises to go to every party they ever throw.
* Played straight in Season 4 of ''WesternAnimation/VoltronLegendaryDefender'' when [[spoiler: Pidge's search for her brother leads her to a massive memorial on the site of a past battle and a grave with his name on it.]] [[spoiler: He turns out to be alive, but her breakdown and agonized scream at his "grave" still left many a viewer in tears]]
* ''WesternAnimation/ReadyJetGo'': Jet's cousin Zerk screams "NOOOOOOOOO!" at the end of "Whole Lotta Shakin'" after Jet throws his smartwatch away, which means Zerk can no longer bother Jet.
* Yelled by Unikitty in the ''WesternAnimation/{{Unikitty}}'' episode "Sparkle Matter Matters".
-->'''Dr. Fox:''' This kid is eating all alone.\\
'''Unikitty:''' Oh no! That's really sad! Where's all of his friends?\\
'''Dr. Fox:''' They didn't show up to his birthday party.\\
'''Unikitty (turns blue):''' Oh no! It's his birthday and no one's there?\\
'''Dr. Fox:''' And the kid is actually... Puppycorn!\\
'''Unikitty (blue):''' Little bro! NOOOOO!!!!
* Essentially a CatchPhrase for Grup in ''WesternAnimation/MightyMagiswords''.