* WebVideo/{{Hadriex}} at the end of his ''VideoGame/SimFarm'' review.
* [[http://twitpic.com/4qe1w4 This Photoshop comic]] parodying A&W's commercials shows Ryan throwing Allen a birthday party. Allen invokes this trope when Ryan announces he's giving away free [[MacGuffin Mama Burgers]].
* [[https://www.youtube.com/watch?v=Eal4fep7pK4 Big No now comes in a handy dandy compilation clip on]] YouTube! Order yours TODAY!
** Also in reaction videos.
* Necro Critic did this when he found out there was more to ''{{Ehrgeiz}}'' than just the terrible fighting game. This one lasted past his 30-second long end screen and continued even after stopping for air.
* The end of the season 3 finale of ''AHDotComTheSeries'': after it's revealed that they inadvertently overwrote the location of the Planet of the Nymphomaniacs in the ship's memory banks, the entire crew lets out a Big No so loud it literally echoes off distant galaxies and is heard in other dimensions.
* A [[MemeticMutation popular internet meme]] is RAEG, where a template comic strip is drawn on, with the ending of a grotesque face shouting "FFFFUUUU--" in situations like a no smoking sign, being caught watching porn, etc.
* ''TheLeetWorld'''s Chet usually greets the death of his drinking buddy Player with a cry of "Player, NOOOOO!" However, since the show is set inside a VideoGame, dead characters are usually [[DeathIsCheap back in action]] next round. A notable exception is when Player is injured by Ahmad's grenade, as this took place outside the challenge levels where [[KilledOffForReal death is permanent]]. {{Lampshade|Hanging}}d in the episode "Thunderhead" when Player [[LeeroyJenkins breaks cover runs into the line of fire]]. Westheimer shouts "Player, No!" and Chet comments that it sounds better when he says it.
* ''WebVideo/TheAngryVideoGameNerd'': In the "[[YetAnotherChristmasCarol Christmas Carol]]" episode: "NOOOOOOO! NO! NO! NO!", when the Ghost of Christmas Future shows him his tombstone, in the style of ''VideoGame/CastlevaniaIISimonsQuest''.
* In the ''WebAnimation/ZeroPunctuation'' review of ''Spore'', Yahtzee delivers a deadpan Big No. Twice. Both times in his inimitably snarky manner.
-->"So can his new game Spore possibly live up to that legacy? In short, no. In long, noooooooooooooo..."''[[OverlyLongGag (total length: 17 seconds)]]''"...ooo.\\
So I guess what I'm asking is, 'Can brightly coloured monstrosities really compare to Batman?' Short answer, Nooooooooo..." ''(interrupted by closing music)''
* Julian and Tarquin from ''The Chin Review'' [[http://www.youtube.com/watch?v=vXnmJkfNWhY&feature=related are really, really attached to their cheese and wine.]]
* In the ''WhateleyUniverse'', Phase does this once, upon learning something horrific about herself during powers testing.
* At the end of the [[http://www.gametrailers.com/video/the-best-screwattack/29711 Five Best and Worst Sonic Games]] countdown, Stuttering Craig does one, when he finds he can't turn off the music to ''[[SonicTheHedgehog Sonic R]]''.
* WebVideo/TheNostalgiaChick does this on being exposed to the Christmas Shoes song in her "Top 10 Disturbing and Inescapable Christmas Songs" special. WebVideo/TheNostalgiaCritic also shouts this frequently. A notable one is when he finally finds out that ''Film/TheStarWarsHolidaySpecial'' does, in fact, exist... and that he has to review it.
-->'''Nostalgia Critic:''' No... it's not true... ''it's impossible!!!''\\
'''Darth Vader:''' Search your feelings, you know it to be true!\\
'''Nostalgia Critic:''' NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!!!!!!!!!!
** She also does it when [[spoiler: [[DroppedABridgeOnHim Nella dies by "bridge drop"]]]] during the Kirk VS Picard review.
** WebVideo/TheNostalgiaCritic gives a ''series'' of these in his ''DropDeadFred'' review. Some of them are comparatively [[LittleNo small "no"s]], but he makes up for it with quantity.
** WebVideo/TheNostalgiaCritic has "I HAVE A NEGATIVE RESPONSE TO THIS SCENARIO!!! I HAVE A NEGATIVE RESPONSE TO THIS SCENARIO!!!" This while making fun of ''Film/{{Tombstone}}'''s Big No. However he actually lists this trope as one of his favorite clichés.
** Rob (dressed as Emperor Palpatine) tells [[WebVideo/TheNostalgiaCritic Doug]] (dressed as Darth Vader) that he must review the 1999 animated movie ''TheLegendOfTheTitanic''. Guess the result.
** At the end of ''The Odd Life of Timothy Green'', when the Critic hits his RageBreakingPoint...again:
--->'''Critic''': NO! [pulls down technicolour swirly image over the camera, bearing the word "NO!" in big red letters, then waves a hand in front of it] NOOOOOOOOO!
** Nostalgia Critic also discussed it in the Top 11 Coolest Cliches as "Screaming 'No!'"
* In ''TheBatterysDown'' Courtney has one of these when Jake says he's quitting musical theatre.
* ''LetsPlay/{{Raocow}}'' [[http://www.youtube.com/watch?v=2MpC17vFGkI#t=7m30s did this seemingly unintentionally.]] Made extra hilarious by how loud, deep, and scary it is compared to his regular voice.
* Also from a [[LetsPlay Let's Player]], {{LetsPlay/Chuggaaconroy}}'s occasional Epic No. To be fair, it isn't a long, drawn out "NOOOOOOOO" as much as a deep, harsh "NO!", which in context is a rejection, not the cry of despair seen in movies/other media. Chuggaaconroy parodies himself in [[http://www.youtube.com/watch?v=9olvSnGn9sg this video]] at about 11:46. "And there's Luigi's Epic NOSE!"
** Though he does have "cries of despair" frequently, particularly when he's joined with the rest of TheRunawayGuys.
* [[LetsPlay Let's Plays]] of ''VideoGame/IWannaBeTheGuy'' will often have this. Even the creator of the game himself [[http://www.youtube.com/watch?v=T27w7LhiUkk&t=5m17s has been pushed this far.]] [[http://www.youtube.com/watch?v=ih_ZPaUvGDM&t=6m35s Here's a much more dramatic one]]
** As does [[SonicTheHedgeHog2006LetsPlay Let's Play Sonic 2006]].
--> NO! NOOOOOO!
* In ''ShadowOfIsraphel'', Simon Lane gives one upon discovering his and Lewis Brindley's Yogcave had been [[http://www.youtube.com/watch?v=2Gi9-UZKYo4&t=9m26s annihilated]], complete with an epic zoom out.
* ''FiveSecondFilms'' has a fourth wall-straining example (also counting as a case of SkywardScream) in "[[http://5secondfilms.com/watch/boarding_school Boarding School]]". Lampshaded in the description:
-->"Unfortunately for Junior, he's getting shipped to St. Understated, Thoughtful Conclusions Academy. Life's gonna be rough."
** Also a quieter-than-usual (but ''angry'') example as an opinion on [[http://5secondfilms.com/watch/planking/ planking]].
* ''TheAnnoyingOrange'': Orange at the end of ''[[http://www.youtube.com/watch?v=Xp9f7Gn4AQw More Annoying Orange]]'' and Cheese at the end of ''[[http://www.youtube.com/watch?v=VEH3u2Qz4fc A Cheesy Episode]]''.
* [[WebVideo/TheSpoonyExperiment Spoony]] reacts this way in the "Party Mania" review first to suffer an eternity of a party in Canada and the second time when he will lose his 'wiener'.
** He mocks the use of one in ''Tekken: Blood Vengeance'', arguing that they hadn't really reached the Noooooo! stage of a relationship given their three minutes of screentime.
* [[http://nooooooooooooooo.com/ NOOOOOOOOOOOOOO!!!!!]]
* Two in ''Machinima/WaterHuman'', both very dramatic and ending abruptly. First, the Water-Human cries out over [[spoiler:David Hasselhoff]]'s corpse, before realizing that it's weird to angst so much over a guy he knew for less than a minute. Later on Water-Human and a prison warden cry over the Large Beetle, who claims to have a heart attack, but then reveals he was just kidding.
* WebVideo/ChadVader does this when he finds out that his girlfriend Libby has been deported, [[MoodWhiplash only for the Marshmallow Bandito to harass him]].
-->'''Chad:''' You picked the ''wrong'' day to mess with me, nerd! ''(beats him up)''
* ''LetsPlay/MarikPlaysBloodlines'':
-->'''Marik:''' I'll just work on my tan. Wait. I'm a vampire. I no longer have a tan. NOOOOOOOO!
* The end of the "Sloppy Roethlisberger" episode of ''WebVideo/EpicMealtime'' has Harley accidentally breaking and spilling an entire bottle of Jack Daniels, with this being the appropriate response from someone in the background.
* A couple of times throughout Nakar's Let's Play Ultima series, but most memorably right at the end of [[http://lparchive.org/Ultima-4-5-and-6/Update%2057/ Ultima VI]].
-->'''Beh Lem:''' "To be saying nooooooooooooo in slow motion!"
-->'''Death Cannon:''' [[TakingTheBullet *BOOM*]]
* ''LenoreTheCuteLittleDeadGirl'': After Lenore dies, Ragamuffin shouts one while [[TearJerker he begs to Lenore's corpse to not leave him.]]
** The Ladybug from Last Dance of Ladybug also gives one after she was injured by a kid.
* Twice in ''WebVideo/TwoBestFriendsPlay''. One was Pat's response to the thought of paying for all of ''Vampire Rain'', and the other is when Matt found out they were going to play ''Shaq Fu''.
* LetsPlay/{{Gronkh}} often shows this reaction, for example on various occasions when being killed in ''VideoGame/{{Minecraft}}'', (most often by a [[ActionBomb creeper]], for example [[http://www.youtube.com/watch?v=r5FqRjUpa-g here]]).
* ShinyObjectsVideos: In "Fishsticks", being the WorldOfHam that it is, a BigNo was all but inevitable.
* TomSka has a NO! moment while discussing the post-production of the then-impending video "Holy Shit, Cats!"
-->'''Tom''': ...then I have to send it over to Andrew, who puts the special effects in--...\\
'''Jenny''': ''*produces Tom's "Dubstep Sunglasses" and puts them on. Pop music plays instead*''\\
'''Tom''': NO!! NOOO!! NOOOOO!! ''*video cuts to the end*''\\

* In the ''TeamFourStar'' LetsPlay of the ''VideoGame/Left4Dead2'' custom campaign "Detour Ahead." Just after barely surviving most of the finale, Gan gets two in:
--> '''Lani''': OH MY GOD A TANK!
--> '''Gan''': NOOOOOOOOOOOOOOOO! NOOOOOOOOOOOOO!
** Said tank promptly killed Kaiser and incapped the rest of the team within 7 seconds of Lani's warning.
* Cloud8745 from Website/{{Youtube}} provided a [[http://www.youtube.com/watch?v=-v96TbsKDqU#t=291s pretty epic one]] during a [[SelfImposedChallenge challenge run of]] ''[[{{Kirby}} Kirby's Dream Land]]''
* The Canadian [[Star Trek]] web series parody, Star Track: Idomo has some Big NOs!
** Captain Tremblay said it when the Blorg destroyed his planet, and when Geordi La Farge dies in main engineering.
** Kuval screams it when the Christmas Messiah is beamed away.
** The Orion captain screams it when Tremblay kills him.
** The evil genecist screams it when Yeoman Bland destroys all his clones or (his calones).
** Rhian screams it when Kuval rams his bird of prey to his warbird.
** Captain Fortune screams it when his wife dies.
* One episode of ''WebVideo/GameGrumps'' had Creator/{{Egoraptor}} doing this after JonTron calls a Pokemon by the wrong name.
-->'''Jon''': Oh, it's a Pumbloom![[note]]It's actually named a Whismur.[[/note]]
-->'''Ego''': Do you remember the episode where Ash catches a Pumbloom?
-->'''Jon''': Dude-
-->'''Ego''': (Angrily) '''NO!!!'''
** Ego get another one in ''VideoGame/{{Castlevania|I}}'', after a skeleton enemy accidentally leaps into a bottomless pit.
-->'''Ego''': BOOOONES! ''NOOOOOOOOO!!!''
** Jon does this twice: when he gets hit by a Mini-Necky on his last life in ''VideoGame/DonkeyKongCountry2DiddyKonsQuest'', and when he endures the {{Nintendo Hard}}ness of ''[[VideoGame/NintendoLand Donkey Kong's Crash Course]]''.
* ReverseJurassicPark: Mr. Robustus in the fivequel.
--> '''Mr. Robustus''': NOOOOOOO!!!!!!!
* In ''WebVideo/ImAMarvelAndImADC'', Darkseid gets one after [[spoiler:discovering he is a fictional character and an action figure in Zero Hour.]]
* ''WebVideo/AtopTheFourthWall'': Linkara's reaction to some oddness involving Wolverine in ''ComicBook/{{Marville}}'' is "One, no. Two, ''God'' no. Three, that was a ''terrible'' pun. Four, '''NO NO NO!'''"
* ''WebVideo/ToddInTheShadows'': his response to imagining the Black-Eyed Peas' "The Time (Dirty Bit)" redone with other eighties music is to scream "NO!" with increasing volume after each clip.