* ''WebComic/EightBitTheater'': [[TheMunchkin Red Mage]] uses this as Black Mage is about to burn the Rat's Tail they need for the class change. He puts on a levitation spell so he can do the slo-mo jump (but applies too much and is left hanging for a while), and does the Big No just to be dramatic.
* ''Webcomic/DarthsAndDroids'' has several of these.
* ''Webcomic/ElGoonishShive'' has multiple examples playing with this trope.
** Elliott does this when he [[http://egscomics.com/?date=2002-07-09 learns that his ex-girlfriend Nanase is the cousin of his friend Tedd]].
** Tedd's father uses this [[http://www.egscomics.com/?date=2006-02-13 when he's realizes]] he's said he'll pay for eight teenagers to eat pizza, while at a meeting far away. The person he's talking to thinks her ideas are being criticized. The comic is helpfully titled 'I Graduated from the GeorgeLucas Directing Academy'.
** Justin makes this exclamation when [[http://www.egscomics.com/?date=2012-06-26 he feels a great disturbance in the Force while Nanase's hair is being cut off]].
** Justin [[http://egscomics.com/?date=2012-08-31 does it again]] when he needs [=DVDs=] of the original ''Franchise/StarWars'' movies but Tedd only has [=DVDs=] of the Special Edition.
* Subverted in the third panel of [[http://www.sluggy.com/daily.php?date=041116 this]] strip of ''Webcomic/SluggyFreelance'', where a "{{little no}}" gives the dramatic impact a Big No is supposed to have.
* ''Webcomic/TheOrderOfTheStick''
** Vaarsuvius uses it in [[http://www.giantitp.com/comics/oots0504.html strip #504]], titled "[[LampshadeHanging That's a Surprisingly Common Reaction]]". {{Lampshaded}} in the first panel of the next strip, by GenreSavvy Elan:
--->'''Elan:''' Hey, did someone in here just drop to their knees and scream, "Noooooo"? I got a feeling....
** Durkon gets one of these in ''On the Origine of [=PCs=]'' after tasting what is allegedly the [[ATankardOfMooseUrine "finest beer brewed in all the human lands."]]
** And the intermission comic, where... well just [[http://www.giantitp.com/comics/oots0301.html see for yourself]].
** V does it again after [[spoiler:an as-of-yet unnamed dragon swears to kill V's kids]].
** Xykon, of all people, does it when his [[spoiler:phylactery is nearly lost to the Snarl]].
--->'''Xykon:''' No no no no no no NO!\\
[...]\\
'''Xykon:''' YES!
** Tsukiko gets one [[spoiler:once she realizes that her fate has been sealed.]]
* ''CaptainSNES'' has had its fair share of them:
** Dr. Wily does the traditional NOOOOOOOOOOOOOO! When he realises he's in a catch 22 as the VP of Nexus and doing anything to ruin Nexus would cause Blues to torture him, meaning he would have to go against his nature and save it from the stupidity that was President Haggar.
** Alex Willams, replaces the Big No with the Big FUCK, and purposely extends it to buy time to think.
** He ''did'' a simple Big No before. It lasted something like ''thirteen comics''.
* ''[[{{Walkyverse}} Shortpacked!]]'':
** This is [[http://www.shortpacked.com/d/20080314.html Robin's reaction]] to finding out that [[Series/BattlestarGalacticaReimagined Felix Gaeta]] is portrayed by the same man that voiced [[TheScrappy Nightscream]] from ''{{Transformers}}: Beast Machines''.
** This is also Amber's reaction to [[FridgeHorror finding out]] that [[spoiler: she may be related to Faz.]] It takes her two comics.
* ''Webcomic/GirlGenius''
** Plays... oddly when Gilgamesh Wulfenbach is [[http://www.girlgeniusonline.com/comic.php?date=20050110 led to believe that Agatha was killed]] by a rogue clank. He begins the wind-up to a Big No before cold logic takes over. However, in a flashback to the same scene, Gil is in the classic [[http://www.girlgeniusonline.com/comic.php?date=20050912 "Big No" pose]]. So it's unclear whether he did one or not.
** Many ''Girl Genius'' characters get a Big No, of course. Gil demonstrates correct form [[http://www.girlgeniusonline.com/comic.php?date=20030704 here]].
* ''{{Candi}}'' presents the rare [[http://www.candicomics.com/d/20080528.html quixotically hopeful drunken ex-boyfriend]] version.
* ''SilentHillPromise'': When Vanessa is grabbed into a pit by a...thing.
* ''TodayNothingHappened'': This very wiki is responsible in [[http://www.shazzbaa.com/index.php?c=3 this strip]].
* ''BobTheAngryFlower'' has it at the end of [[http://www.angryflower.com/hamste3.html this strip.]]
* Used pretty straight in ''FinalBlasphemy'' [[http://www.drunkduck.com/Final_Blasphemy/index.php?p=440339 here]] and later in the same issue.
* In ''StarslipCrisis'', [[http://www.starslip.com/archive/20080905.shtml Zillion can't even]] do ''this'' right.
* In ''TheInexplicableAdventuresOfBob'' when Bob learns [[http://bobadventures.comicgenesis.com/d/20061003.html his roof is about to be destroyed]] [[http://bobadventures.comicgenesis.com/d/20061005.html a third time.]] It will happen [[RunningGag many more times.]]
** Riboflavin gets one when he faces a [[http://bobadventures.comicgenesis.com/d/20110201.html fate worse than death.]]
* From ''{{Multiplex}}'', [[http://www.multiplexcomic.com/archive.php?name=249 sarcastically, from Kurt]].
* [[http://www.smbc-comics.com/index.php?db=comics&id=1362 Candice didn't appreciate his creative marriage proposal.]]
* Happens in ''CornerAlley13'' when Noelle notices that [[http://corneralley.comicgenesis.com/d/20050527.html her café doesn't have any customers]].
* Right [[http://elvenlacryment.com/?p=56 here]] in ''ElvenLacryment''.
* ''Webcomic/DragonMango'' [[http://www.dragon-mango.com/comic/chapter05/dm05-10.htm spoofs]] this when Pumpkin's father returns after a four-year journey into faraway lands and gives Mango a rare treat, called a "sweet swirl stick", which Cherry eats in one bite.
* Jason from ''Webcomic/SomethingPositive'' has a fairly [[http://www.somethingpositive.net/sp10152009.shtml unique]] one when realizing he's just turned down a 3-way with his wife and hot friend.
* Rhea of ''SlightlyDamned'''s [[http://www.sdamned.com/2005/10/10042005/ reaction]] upon learning that she cannot be reincarnated.
* ''PowerpuffGirlsDoujinshi'' has the following conversation.
-->'''Professor Utonium:''' What's that you got there? Is that a... Wh--Th--P...P....\\
[[spoiler:'''Buttercup:''' Parent-Teacher Conference...]]\\
'''Professor Utonium:''' [[SkywardScream NNNNNOOOOOOOOOOOOOOOOOOOOOOOO!!!]]
* A [[http://www.jaydenandcrusader.com/2010/02/22/page-149/ Nooooo!]] that bursts out of the panel in ''Jayden and Crusader''.
* Cale whips one out in [[http://www.lfgcomic.com/page/354 a comic]] of ''Webcomic/LookingForGroup''.
* ''Webcomic/DanAndMabsFurryAdventures'' has [[http://www.missmab.com/Comics/Abel_25.php this]] beauty when a young Abel learns that extra lessons include extra homework.
* ''Webcomic/PennyArcade'': Gabriel provides this [[http://www.penny-arcade.com/comic/2008/4/11/ gem]] upon besting Tycho in a card game.
* In ''Webcomic/BrawlInTheFamily'', Kirby unleashes one when [[FelonyMisdemeanor Meta Knight takes his revenge on him]] [[http://www.brawlinthefamily.com/?p=627 here.]]
* Monica from ''Webcomic/WapsiSquare'' had a nice one when [[spoiler:Phyx decided to "tag" Nudge.]]
* The Princess of ''Webcomic/RustyAndCo'' when she [[http://rustyandco.com/comic/level-4-title/ found]] the White Knight crushed.
* ''Webcomic/{{Loserz}}'' parodied the original scene [[http://the-qlc.com/loserz/go/52 here.]]
* ''Webcomic/DorkTower'' [[http://www.dorktower.com/2001/01/18/comics-archive-45/ audible five miles away]]
* [[AuthorAvatar Chris]] in ''Webcomic/{{Sonichu}}'' does this when he learns that a Starbucks barista only went out with him as a joke. [[RealLifeWritesThePlot Apparently, this happened in real life.]]
* [[http://whatsshakincomic.com/2011/07/18/page-44/ Nith]] from ''WhatsShakin'' discovers his home has been destroyed.
* ''Webcomic/MenageA3'' is quite prone to this. For example:
** Kiley [[http://www.menagea3.net/strips-ma3/i_wish_i_were_a_woman here.]]
** Zii's reaction when DiDi tries to cut her hair into a mullet.
** Zii again [[http://www.menagea3.net/strips-ma3/save_me_-_guest here]] and [[http://www.ma3comic.com/strips-ma3/show-accuracy here]].
** Kiley again [[http://www.menagea3.net/strips-ma3/seriously_strained here]] when she thinks Yuki is going to see her father in Japan when she's in a bad condition, and yet again [[http://www.menagea3.net/strips-ma3/english_os_restored here]].
* ''Webcomic/{{Mulberry}}''
** Mulberry says one after [[spoiler:landing in rehab after some activities she performed during an [[IntoxicationEnsues accidental drug ingestion]].]]
** She performs [[http://www.platypuscomix.net/mulberry/index.php?issue=20&page=4 another one]] after Jack shows her a newspaper article detailing the death of Creator/BrittanyMurphy.
* ''Webcomic/PrincessPi''
** AlphaBitch Sam yells this after getting sentenced to [[spoiler:community service]] as punishment for blowing up Piscataway Stadium.
** Princess Pi herself [[http://www.platypuscomix.net/princesspi/index.php?issue=9&page=8 says this]] when the new princess, Gertie, makes her genie, Bottle Blonde, encase her own bottle in concrete, so that Pi could not make Bottle Blonde turn Piscataway back to normal.
* ''Webcomic/TrueBelievers'' has one after [[spoiler:[[Franchise/SpiderMan ]] uses a stolen stamp to give [[Creator/JoeQuesada Joe Quesadilla]] a RetGone]].
* In ''{{Shifters}}'' Ben Harkus gives us one of these [[http://shifters.comicgenesis.com/d/20021202.html here]] after [[spoiler:[[http://shifters.comicgenesis.com/d/20021202.html failing to catch Ferrah and letting her fall from a crashed plane]] that had been [[http://shifters.comicgenesis.com/d/20021120.html brought down by Maverick]].]]
* [[http://hiddendepths.smackjeeves.com/comics/1356584/001-bulbasaur/ Bulbasaur]], after dropping a little girl in a [[DrowningMySorrows failed rescue attempt]] in ''151 Hidden Depths''.
* In ''Webcomic/CucumberQuest'', Cucumber delivers one as Sir Tomato brings his sword down on him.
* {{Sinfest}}
** [[http://www.sinfest.net/archive_page.php?comicID=1395 Big No from Lil' E]].
** Slick [[http://www.sinfest.net/archive_page.php?comicID=3732 REALLY]] should have saved frequently.
* Pitr of ''UserFriendly'' combined this with FakeRussian when geeks shot him from moon into the Google building: [[http://ars.userfriendly.org/cartoons/?id=20040415 NYYYYEEEEEEEEEEEEEEEEEEEET!!]]
* Hazel of ''Webcomic/GirlsWithSlingshots'' does a quiet version of this. [[http://www.girlswithslingshots.com/comic/gws-1495/]]
* In ''Webcomic/GunnerkriggCourt'', the first time Antimony meets the {{Psychopomp}}s, she does this when seeing a dying child's memeory of accidentally burning his house.
* ''Webcomic/{{Homestuck}}'': oh gog.
-->'''[[spoiler:Trickster Mode]] Jane''': I feel better than alright! I feel great! I feel so alive! I feel so...[[spoiler: CAUCASIAN!]]\\
'''Jake''': NOOOOOOOOOOOOOOOOOOOOOOOOOOOO
** Kanaya's response to [[spoiler: Rose asking if she was going to break up with her]] was a frustrated no punctuated by shoutpoles (!).
** [[http://www.mspaintadventures.com/?s=6&p=007918 Terezi]] gives one punctuated by crying when Karkat points out that [[spoiler: Gamzee]] will likely leave her.
** Taking this to increasingly {{exaggerated|Trope}} levels seems to have become a running gag. John's big No after [[spoiler: a Mind-Controlled Jane kills Karkat]] takes so long he has to pause for breath at the end of each page before continuing, [[AngstWhatAngst only for him to immediately perk back up when Jane revives Karkat four pages later.]] Later, Dave's big no is so long that it stretches down to where his apartment building [[ConvectionSchmonvection reaches the lava of LOHAC]] after [[spoiler: Grimbark Jade kicks WV over the edge of the building.]]
* At the conclusion of the ''Frollo Beats Up Evil Residents'' arc in ''TheFrolloShow'', Frollo lets out one after [[spoiler: Jafar launches a very, very slow moving projectile at him.]]
* In Webcomic/AvasDemon, [[http://www.avasdemon.com/pages.php?page=0530 Odin's reaction]] to [[http://www.avasdemon.com/pages.php?page=0529 seeing his ship.]]
* ''Webcomic/PvP'''s Francis Ottoman yelled one when Robbie announced that [[http://www.pvponline.com/comic/2007/07/17/jul-17-2007 the party was over]].
* Ballister gets one in ''Nimona'' when [[spoiler: a guard cuts Nimona's head off.]]
* In ''Webcomic/SandraOnTheRocks'', Tatiana invokes the short, intense version [[http://www.sandraontherocks.com/strips-sotr/sex_the_other_into_submission here]] when she discovers that something ... terrible ... may have happened to her beloved boss Domenico.
* In [[http://questionablecontent.net/view.php?comic=2652 Questionable Content]], this is Faye's reaction to [[spoiler: discovering she's officially a New Englander]].
* ''Webcomic/BrunoTheBandit'':
** Bruno's mother yelled one when her father threatened to turn her granddaughter into a [[http://www.brunothebandit.com/d/20001129.html fame vampire]].
** The spirit of Maledict's brother-in-law yelled one when the ritual intended to transfer it into Bruno's body [[http://www.brunothebandit.com/d/20010602.html failed completely]].
** One of Bran-at-Morn's men yelled one when the Asutonians threatened to burn up their entire [[http://www.brunothebandit.com/d/20011101.html laxative supply]].
* In ''Webcomic/{{Oglaf}}'', believers in [[GodIsEvil Sithrak]] have good reason to [[http://oglaf.com/outreach/ take deaths especially hard]]. (SFW link to a frequently very NSFW comic.)
* In ''Webcomic/FlintlockesGuideToAzeroth'', we get this:
-->'''Flintlocke:''' Yeah, har har! Lowping, we beat down this 'ere shaman an' now [[spoiler:yeh only 'ave one hit point left]]!
-->'''Lowping:''' [[{{Catchphrase}} I lewt the hat.]]
-->'''Flintlocke:''' [[spoiler:Jes' one hit point!]] Har har! That were awesome!
-->[[spoiler:*Bun'kar sneaks up and nibbles away Lowping's last hit point.*]]
-->'''Flintlocke:''' '''''[[SkywardScream NOOOO]][[RoaringRampageOfRevenge OOOO!!]]'''''
* In ''Webcomic/KnightsOfBuenaVista'', Mary has her PlayerCharacter do this when another PC is stabbed (it was only 3 damage, but the character wouldn't know that).
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